This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.09 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 228,700 (10.2%) Reads that were too short: 12,760 (0.6%) Reads written (passing filters): 215,940 (9.6%) Total basepairs processed: 673,916,425 bp Total written (filtered): 61,584,431 bp (9.1%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 228700 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2134 546.6 0 2134 7 115129 546.6 0 115129 8 81329 546.6 0 81329 9 98 546.6 0 98 10 32 546.6 0 32 11 24 546.6 0 24 12 27 546.6 0 27 13 22 546.6 0 22 14 18 546.6 0 18 15 25 546.6 0 25 16 24 546.6 0 24 17 28 546.6 0 28 18 114 546.6 0 114 19 29 546.6 0 29 20 88 546.6 0 88 21 32 546.6 0 32 22 36 546.6 0 36 23 18 546.6 0 18 24 21 546.6 0 21 25 33 546.6 0 33 26 39 546.6 0 39 27 45 546.6 0 45 28 23 546.6 0 23 29 22 546.6 0 22 30 33 546.6 0 33 31 24 546.6 0 24 32 25 546.6 0 25 33 31 546.6 0 31 34 36 546.6 0 36 35 30 546.6 0 30 36 28 546.6 0 28 37 25 546.6 0 25 38 37 546.6 0 37 39 29 546.6 0 29 40 23 546.6 0 23 41 36 546.6 0 36 42 18 546.6 0 18 43 28 546.6 0 28 44 35 546.6 0 35 45 34 546.6 0 34 46 30 546.6 0 30 47 28 546.6 0 28 48 30 546.6 0 30 49 24 546.6 0 24 50 27 546.6 0 27 51 22 546.6 0 22 52 35 546.6 0 35 53 31 546.6 0 31 54 46 546.6 0 46 55 37 546.6 0 37 56 29 546.6 0 29 57 34 546.6 0 34 58 34 546.6 0 34 59 49 546.6 0 49 60 26 546.6 0 26 61 46 546.6 0 46 62 24 546.6 0 24 63 28 546.6 0 28 64 28 546.6 0 28 65 39 546.6 0 39 66 52 546.6 0 52 67 61 546.6 0 61 68 60 546.6 0 60 69 118 546.6 0 118 70 190 546.6 0 190 71 2111 546.6 0 2111 72 433 546.6 0 433 73 1075 546.6 0 1075 74 38 546.6 0 38 75 40 546.6 0 40 76 34 546.6 0 34 77 65 546.6 0 65 78 43 546.6 0 43 79 172 546.6 0 172 80 44 546.6 0 44 81 52 546.6 0 52 82 42 546.6 0 42 83 36 546.6 0 36 84 45 546.6 0 45 85 70 546.6 0 70 86 44 546.6 0 44 87 85 546.6 0 85 88 44 546.6 0 44 89 17 546.6 0 17 90 48 546.6 0 48 91 38 546.6 0 38 92 29 546.6 0 29 93 43 546.6 0 43 94 97 546.6 0 97 95 158 546.6 0 158 96 710 546.6 0 710 97 373 546.6 0 373 98 550 546.6 0 550 99 57 546.6 0 57 100 26 546.6 0 26 101 25 546.6 0 25 102 22 546.6 0 22 103 30 546.6 0 30 104 39 546.6 0 39 105 36 546.6 0 36 106 27 546.6 0 27 107 28 546.6 0 28 108 39 546.6 0 39 109 32 546.6 0 32 110 26 546.6 0 26 111 30 546.6 0 30 112 36 546.6 0 36 113 32 546.6 0 32 114 43 546.6 0 43 115 45 546.6 0 45 116 50 546.6 0 50 117 53 546.6 0 53 118 74 546.6 0 74 119 43 546.6 0 43 120 30 546.6 0 30 121 29 546.6 0 29 122 50 546.6 0 50 123 54 546.6 0 54 124 144 546.6 0 144 125 905 546.6 0 905 126 320 546.6 0 320 127 70 546.6 0 70 128 94 546.6 0 94 129 93 546.6 0 93 130 106 546.6 0 106 131 46 546.6 0 46 132 75 546.6 0 75 133 64 546.6 0 64 134 60 546.6 0 60 135 91 546.6 0 91 136 48 546.6 0 48 137 54 546.6 0 54 138 50 546.6 0 50 139 50 546.6 0 50 140 75 546.6 0 75 141 76 546.6 0 76 142 53 546.6 0 53 143 62 546.6 0 62 144 55 546.6 0 55 145 50 546.6 0 50 146 54 546.6 0 54 147 37 546.6 0 37 148 42 546.6 0 42 149 56 546.6 0 56 150 47 546.6 0 47 151 46 546.6 0 46 152 40 546.6 0 40 153 43 546.6 0 43 154 45 546.6 0 45 155 71 546.6 0 71 156 55 546.6 0 55 157 41 546.6 0 41 158 42 546.6 0 42 159 44 546.6 0 44 160 37 546.6 0 37 161 38 546.6 0 38 162 32 546.6 0 32 163 39 546.6 0 39 164 48 546.6 0 48 165 79 546.6 0 79 166 67 546.6 0 67 167 45 546.6 0 45 168 54 546.6 0 54 169 40 546.6 0 40 170 56 546.6 0 56 171 51 546.6 0 51 172 101 546.6 0 101 173 303 546.6 0 303 174 124 546.6 0 124 175 308 546.6 0 308 176 67 546.6 0 67 177 55 546.6 0 55 178 44 546.6 0 44 179 33 546.6 0 33 180 66 546.6 0 66 181 47 546.6 0 47 182 37 546.6 0 37 183 41 546.6 0 41 184 46 546.6 0 46 185 77 546.6 0 77 186 72 546.6 0 72 187 72 546.6 0 72 188 42 546.6 0 42 189 51 546.6 0 51 190 67 546.6 0 67 191 127 546.6 0 127 192 458 546.6 0 458 193 302 546.6 0 302 194 547 546.6 0 547 195 125 546.6 0 125 196 40 546.6 0 40 197 56 546.6 0 56 198 52 546.6 0 52 199 63 546.6 0 63 200 81 546.6 0 81 201 64 546.6 0 64 202 51 546.6 0 51 203 59 546.6 0 59 204 92 546.6 0 92 205 72 546.6 0 72 206 62 546.6 0 62 207 96 546.6 0 96 208 50 546.6 0 50 209 112 546.6 0 112 210 90 546.6 0 90 211 174 546.6 0 174 212 143 546.6 0 143 213 58 546.6 0 58 214 53 546.6 0 53 215 81 546.6 0 81 216 38 546.6 0 38 217 87 546.6 0 87 218 61 546.6 0 61 219 92 546.6 0 92 220 99 546.6 0 99 221 92 546.6 0 92 222 53 546.6 0 53 223 53 546.6 0 53 224 83 546.6 0 83 225 281 546.6 0 281 226 84 546.6 0 84 227 182 546.6 0 182 228 109 546.6 0 109 229 59 546.6 0 59 230 62 546.6 0 62 231 64 546.6 0 64 232 72 546.6 0 72 233 75 546.6 0 75 234 56 546.6 0 56 235 60 546.6 0 60 236 73 546.6 0 73 237 81 546.6 0 81 238 81 546.6 0 81 239 62 546.6 0 62 240 91 546.6 0 91 241 111 546.6 0 111 242 86 546.6 0 86 243 62 546.6 0 62 244 43 546.6 0 43 245 53 546.6 0 53 246 84 546.6 0 84 247 86 546.6 0 86 248 94 546.6 0 94 249 101 546.6 0 101 250 56 546.6 0 56 251 49 546.6 0 49 252 60 546.6 0 60 253 54 546.6 0 54 254 124 546.6 0 124 255 179 546.6 0 179 256 129 546.6 0 129 257 83 546.6 0 83 258 107 546.6 0 107 259 129 546.6 0 129 260 85 546.6 0 85 261 90 546.6 0 90 262 69 546.6 0 69 263 97 546.6 0 97 264 74 546.6 0 74 265 86 546.6 0 86 266 99 546.6 0 99 267 150 546.6 0 150 268 153 546.6 0 153 269 93 546.6 0 93 270 97 546.6 0 97 271 208 546.6 0 208 272 250 546.6 0 250 273 212 546.6 0 212 274 397 546.6 0 397 275 181 546.6 0 181 276 114 546.6 0 114 277 83 546.6 0 83 278 122 546.6 0 122 279 244 546.6 0 244 280 2515 546.6 0 2515 281 151 546.6 0 151 282 105 546.6 0 105 283 76 546.6 0 76 284 48 546.6 0 48 285 109 546.6 0 109 286 108 546.6 0 108 287 92 546.6 0 92 288 146 546.6 0 146 289 71 546.6 0 71 290 125 546.6 0 125 291 95 546.6 0 95 292 90 546.6 0 90 293 101 546.6 0 101 294 178 546.6 0 178 295 151 546.6 0 151 296 247 546.6 0 247 297 96 546.6 0 96 298 92 546.6 0 92 299 85 546.6 0 85 300 91 546.6 0 91 301 51 546.6 0 51 Finished in 10.72 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 215,940 Reads with adapters: 83,992 (38.9%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 83,942 (38.9%) Total basepairs processed: 61,584,431 bp Total written (filtered): 22,899,422 bp (37.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 83992 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 6 0.0 3 0 0 0 3 3 18 55 0.0 3 0 0 34 10 11 19 1373 0.0 3 0 1167 96 81 29 20 81631 0.0 4 76611 4049 725 193 53 21 176 0.0 4 21 141 8 4 2 22 23 0.0 4 1 5 10 2 5 23 485 0.0 4 180 11 1 216 77 24 4 0.0 4 0 0 0 2 2 25 3 0.0 4 3 26 2 0.0 4 0 0 0 1 1 27 2 0.0 4 0 0 0 0 2 28 3 0.0 4 1 0 0 0 2 35 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 48 2 0.0 4 0 0 0 0 2 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 107 1 0.0 4 1 110 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 123 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 138 0.0 4 0 0 0 0 138 128 2 0.0 4 1 0 0 0 1 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 1 139 2 0.0 4 0 0 1 0 1 140 1 0.0 4 0 0 0 0 1 141 2 0.0 4 2 152 1 0.0 4 0 0 0 0 1 153 15 0.0 4 0 0 0 0 15 172 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 188 2 0.0 4 0 0 0 0 2 192 1 0.0 4 1 207 3 0.0 4 1 0 0 0 2 208 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 1 222 1 0.0 4 0 0 0 0 1 225 3 0.0 4 0 0 0 0 3 227 2 0.0 4 0 0 0 1 1 228 6 0.0 4 0 0 0 0 6 229 7 0.0 4 0 0 0 1 6 230 1 0.0 4 0 0 0 0 1 233 3 0.0 4 0 0 0 0 3 234 5 0.0 4 0 0 0 0 5 235 4 0.0 4 0 0 0 0 4 236 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.98 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 83,942 Reads with adapters: 15,403 (18.3%) Reads written (passing filters): 83,942 (100.0%) Total basepairs processed: 22,899,422 bp Total written (filtered): 22,652,316 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 15403 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 9.7% G: 82.6% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 138 1311.6 0 138 4 18 327.9 0 18 5 2 82.0 0 2 6 2 20.5 0 2 7 1 5.1 0 1 8 3 1.3 0 3 9 108 0.3 0 5 103 10 2098 0.1 1 185 1913 11 12428 0.0 1 566 11862 12 110 0.0 1 2 108 13 7 0.0 1 0 7 14 17 0.0 1 0 17 15 1 0.0 1 0 1 16 1 0.0 1 0 1 19 12 0.0 1 8 4 20 2 0.0 1 2 21 1 0.0 1 1 23 21 0.0 1 10 11 24 12 0.0 1 9 3 45 1 0.0 1 0 1 79 2 0.0 1 2 81 1 0.0 1 0 1 108 1 0.0 1 1 124 1 0.0 1 1 148 2 0.0 1 2 150 1 0.0 1 1 151 38 0.0 1 24 14 152 4 0.0 1 3 1 157 1 0.0 1 1 175 1 0.0 1 1 190 3 0.0 1 1 2 192 2 0.0 1 0 2 208 1 0.0 1 1 209 313 0.0 1 245 68 210 10 0.0 1 9 1 211 8 0.0 1 5 3 224 7 0.0 1 6 1 225 1 0.0 1 1 231 21 0.0 1 19 2 243 1 0.0 1 0 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.