This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.00 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 228,717 (10.2%) Reads that were too short: 9,130 (0.4%) Reads written (passing filters): 219,587 (9.8%) Total basepairs processed: 673,916,425 bp Total written (filtered): 62,965,414 bp (9.3%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 228717 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1640 546.6 0 1640 7 92569 546.6 0 92569 8 110242 546.6 0 110242 9 93 546.6 0 93 10 33 546.6 0 33 11 22 546.6 0 22 12 18 546.6 0 18 13 31 546.6 0 31 14 19 546.6 0 19 15 16 546.6 0 16 16 29 546.6 0 29 17 32 546.6 0 32 18 45 546.6 0 45 19 30 546.6 0 30 20 33 546.6 0 33 21 26 546.6 0 26 22 42 546.6 0 42 23 25 546.6 0 25 24 21 546.6 0 21 25 22 546.6 0 22 26 25 546.6 0 25 27 25 546.6 0 25 28 24 546.6 0 24 29 28 546.6 0 28 30 33 546.6 0 33 31 21 546.6 0 21 32 29 546.6 0 29 33 24 546.6 0 24 34 34 546.6 0 34 35 37 546.6 0 37 36 45 546.6 0 45 37 32 546.6 0 32 38 35 546.6 0 35 39 28 546.6 0 28 40 27 546.6 0 27 41 29 546.6 0 29 42 31 546.6 0 31 43 24 546.6 0 24 44 39 546.6 0 39 45 29 546.6 0 29 46 24 546.6 0 24 47 30 546.6 0 30 48 23 546.6 0 23 49 20 546.6 0 20 50 24 546.6 0 24 51 26 546.6 0 26 52 26 546.6 0 26 53 29 546.6 0 29 54 36 546.6 0 36 55 35 546.6 0 35 56 31 546.6 0 31 57 19 546.6 0 19 58 39 546.6 0 39 59 36 546.6 0 36 60 28 546.6 0 28 61 34 546.6 0 34 62 29 546.6 0 29 63 32 546.6 0 32 64 36 546.6 0 36 65 31 546.6 0 31 66 22 546.6 0 22 67 52 546.6 0 52 68 54 546.6 0 54 69 107 546.6 0 107 70 141 546.6 0 141 71 2327 546.6 0 2327 72 414 546.6 0 414 73 1268 546.6 0 1268 74 35 546.6 0 35 75 22 546.6 0 22 76 34 546.6 0 34 77 68 546.6 0 68 78 37 546.6 0 37 79 200 546.6 0 200 80 27 546.6 0 27 81 24 546.6 0 24 82 24 546.6 0 24 83 31 546.6 0 31 84 24 546.6 0 24 85 31 546.6 0 31 86 27 546.6 0 27 87 31 546.6 0 31 88 29 546.6 0 29 89 37 546.6 0 37 90 47 546.6 0 47 91 40 546.6 0 40 92 36 546.6 0 36 93 37 546.6 0 37 94 69 546.6 0 69 95 100 546.6 0 100 96 766 546.6 0 766 97 441 546.6 0 441 98 662 546.6 0 662 99 56 546.6 0 56 100 26 546.6 0 26 101 16 546.6 0 16 102 23 546.6 0 23 103 29 546.6 0 29 104 22 546.6 0 22 105 42 546.6 0 42 106 27 546.6 0 27 107 29 546.6 0 29 108 32 546.6 0 32 109 20 546.6 0 20 110 19 546.6 0 19 111 31 546.6 0 31 112 23 546.6 0 23 113 24 546.6 0 24 114 26 546.6 0 26 115 38 546.6 0 38 116 30 546.6 0 30 117 31 546.6 0 31 118 44 546.6 0 44 119 42 546.6 0 42 120 31 546.6 0 31 121 33 546.6 0 33 122 31 546.6 0 31 123 31 546.6 0 31 124 95 546.6 0 95 125 1012 546.6 0 1012 126 243 546.6 0 243 127 55 546.6 0 55 128 32 546.6 0 32 129 38 546.6 0 38 130 69 546.6 0 69 131 39 546.6 0 39 132 32 546.6 0 32 133 38 546.6 0 38 134 36 546.6 0 36 135 52 546.6 0 52 136 44 546.6 0 44 137 33 546.6 0 33 138 24 546.6 0 24 139 34 546.6 0 34 140 31 546.6 0 31 141 67 546.6 0 67 142 49 546.6 0 49 143 25 546.6 0 25 144 35 546.6 0 35 145 31 546.6 0 31 146 30 546.6 0 30 147 30 546.6 0 30 148 32 546.6 0 32 149 35 546.6 0 35 150 20 546.6 0 20 151 38 546.6 0 38 152 27 546.6 0 27 153 37 546.6 0 37 154 39 546.6 0 39 155 41 546.6 0 41 156 35 546.6 0 35 157 46 546.6 0 46 158 28 546.6 0 28 159 25 546.6 0 25 160 46 546.6 0 46 161 30 546.6 0 30 162 25 546.6 0 25 163 38 546.6 0 38 164 24 546.6 0 24 165 42 546.6 0 42 166 23 546.6 0 23 167 37 546.6 0 37 168 27 546.6 0 27 169 27 546.6 0 27 170 24 546.6 0 24 171 41 546.6 0 41 172 62 546.6 0 62 173 167 546.6 0 167 174 70 546.6 0 70 175 165 546.6 0 165 176 30 546.6 0 30 177 36 546.6 0 36 178 35 546.6 0 35 179 31 546.6 0 31 180 37 546.6 0 37 181 25 546.6 0 25 182 32 546.6 0 32 183 29 546.6 0 29 184 29 546.6 0 29 185 32 546.6 0 32 186 43 546.6 0 43 187 43 546.6 0 43 188 26 546.6 0 26 189 36 546.6 0 36 190 52 546.6 0 52 191 76 546.6 0 76 192 417 546.6 0 417 193 195 546.6 0 195 194 489 546.6 0 489 195 77 546.6 0 77 196 30 546.6 0 30 197 37 546.6 0 37 198 24 546.6 0 24 199 40 546.6 0 40 200 36 546.6 0 36 201 30 546.6 0 30 202 30 546.6 0 30 203 43 546.6 0 43 204 91 546.6 0 91 205 37 546.6 0 37 206 33 546.6 0 33 207 69 546.6 0 69 208 32 546.6 0 32 209 32 546.6 0 32 210 33 546.6 0 33 211 44 546.6 0 44 212 44 546.6 0 44 213 39 546.6 0 39 214 34 546.6 0 34 215 36 546.6 0 36 216 26 546.6 0 26 217 48 546.6 0 48 218 26 546.6 0 26 219 39 546.6 0 39 220 30 546.6 0 30 221 45 546.6 0 45 222 52 546.6 0 52 223 41 546.6 0 41 224 50 546.6 0 50 225 392 546.6 0 392 226 48 546.6 0 48 227 154 546.6 0 154 228 32 546.6 0 32 229 44 546.6 0 44 230 21 546.6 0 21 231 25 546.6 0 25 232 22 546.6 0 22 233 46 546.6 0 46 234 36 546.6 0 36 235 39 546.6 0 39 236 36 546.6 0 36 237 32 546.6 0 32 238 59 546.6 0 59 239 41 546.6 0 41 240 32 546.6 0 32 241 53 546.6 0 53 242 24 546.6 0 24 243 30 546.6 0 30 244 36 546.6 0 36 245 22 546.6 0 22 246 25 546.6 0 25 247 59 546.6 0 59 248 41 546.6 0 41 249 48 546.6 0 48 250 40 546.6 0 40 251 30 546.6 0 30 252 39 546.6 0 39 253 35 546.6 0 35 254 63 546.6 0 63 255 73 546.6 0 73 256 106 546.6 0 106 257 55 546.6 0 55 258 52 546.6 0 52 259 62 546.6 0 62 260 40 546.6 0 40 261 52 546.6 0 52 262 55 546.6 0 55 263 70 546.6 0 70 264 37 546.6 0 37 265 118 546.6 0 118 266 68 546.6 0 68 267 48 546.6 0 48 268 77 546.6 0 77 269 61 546.6 0 61 270 61 546.6 0 61 271 184 546.6 0 184 272 144 546.6 0 144 273 94 546.6 0 94 274 173 546.6 0 173 275 83 546.6 0 83 276 65 546.6 0 65 277 51 546.6 0 51 278 87 546.6 0 87 279 173 546.6 0 173 280 3248 546.6 0 3248 281 77 546.6 0 77 282 67 546.6 0 67 283 34 546.6 0 34 284 46 546.6 0 46 285 70 546.6 0 70 286 57 546.6 0 57 287 67 546.6 0 67 288 72 546.6 0 72 289 54 546.6 0 54 290 56 546.6 0 56 291 49 546.6 0 49 292 54 546.6 0 54 293 61 546.6 0 61 294 75 546.6 0 75 295 64 546.6 0 64 296 90 546.6 0 90 297 48 546.6 0 48 298 36 546.6 0 36 299 39 546.6 0 39 300 60 546.6 0 60 301 59 546.6 0 59 Finished in 9.51 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 219,587 Reads with adapters: 91,339 (41.6%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 91,309 (41.6%) Total basepairs processed: 62,965,414 bp Total written (filtered): 25,288,204 bp (40.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 91339 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 8 0.0 3 0 0 8 16 403 0.0 3 0 395 8 17 90562 0.0 3 88875 1620 63 4 18 136 0.0 3 5 123 8 19 15 0.0 3 0 0 11 4 20 148 0.0 3 1 0 0 147 21 1 0.0 3 1 22 1 0.0 3 1 23 1 0.0 3 1 29 1 0.0 3 1 31 2 0.0 3 1 0 0 1 32 2 0.0 3 1 0 0 1 38 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 1 61 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 4 0.0 3 0 0 0 4 76 1 0.0 3 0 0 0 1 80 1 0.0 3 1 85 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 137 2 0.0 3 2 164 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 1 6 193 1 0.0 3 0 0 1 224 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 4 0.0 3 0 0 0 4 281 8 0.0 3 0 0 0 8 282 3 0.0 3 0 0 0 3 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.21 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 91,309 Reads with adapters: 337 (0.4%) Reads written (passing filters): 91,309 (100.0%) Total basepairs processed: 25,288,204 bp Total written (filtered): 25,287,147 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 337 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.8% C: 19.3% G: 19.3% T: 5.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 302 1426.7 0 302 4 27 356.7 0 27 5 5 89.2 0 5 6 3 22.3 0 3