This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.93 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 283,660 (12.7%) Reads that were too short: 4,773 (0.2%) Reads written (passing filters): 278,887 (12.5%) Total basepairs processed: 673,916,425 bp Total written (filtered): 79,183,578 bp (11.7%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 283660 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2203 546.6 0 2203 7 115509 546.6 0 115509 8 2828 546.6 0 2828 9 131054 546.6 0 131054 10 102 546.6 0 102 11 53 546.6 0 53 12 46 546.6 0 46 13 36 546.6 0 36 14 30 546.6 0 30 15 38 546.6 0 38 16 73 546.6 0 73 17 83 546.6 0 83 18 121 546.6 0 121 19 38 546.6 0 38 20 24 546.6 0 24 21 36 546.6 0 36 22 40 546.6 0 40 23 33 546.6 0 33 24 26 546.6 0 26 25 32 546.6 0 32 26 32 546.6 0 32 27 26 546.6 0 26 28 29 546.6 0 29 29 36 546.6 0 36 30 33 546.6 0 33 31 27 546.6 0 27 32 49 546.6 0 49 33 55 546.6 0 55 34 32 546.6 0 32 35 43 546.6 0 43 36 40 546.6 0 40 37 37 546.6 0 37 38 43 546.6 0 43 39 39 546.6 0 39 40 17 546.6 0 17 41 35 546.6 0 35 42 44 546.6 0 44 43 58 546.6 0 58 44 37 546.6 0 37 45 39 546.6 0 39 46 101 546.6 0 101 47 495 546.6 0 495 48 254 546.6 0 254 49 37 546.6 0 37 50 36 546.6 0 36 51 53 546.6 0 53 52 54 546.6 0 54 53 26 546.6 0 26 54 39 546.6 0 39 55 43 546.6 0 43 56 46 546.6 0 46 57 25 546.6 0 25 58 37 546.6 0 37 59 38 546.6 0 38 60 36 546.6 0 36 61 36 546.6 0 36 62 60 546.6 0 60 63 62 546.6 0 62 64 35 546.6 0 35 65 42 546.6 0 42 66 35 546.6 0 35 67 41 546.6 0 41 68 36 546.6 0 36 69 43 546.6 0 43 70 52 546.6 0 52 71 47 546.6 0 47 72 43 546.6 0 43 73 40 546.6 0 40 74 58 546.6 0 58 75 52 546.6 0 52 76 65 546.6 0 65 77 56 546.6 0 56 78 77 546.6 0 77 79 281 546.6 0 281 80 189 546.6 0 189 81 382 546.6 0 382 82 102 546.6 0 102 83 186 546.6 0 186 84 97 546.6 0 97 85 120 546.6 0 120 86 86 546.6 0 86 87 79 546.6 0 79 88 80 546.6 0 80 89 65 546.6 0 65 90 87 546.6 0 87 91 112 546.6 0 112 92 146 546.6 0 146 93 211 546.6 0 211 94 3597 546.6 0 3597 95 5120 546.6 0 5120 96 4661 546.6 0 4661 97 561 546.6 0 561 98 450 546.6 0 450 99 41 546.6 0 41 100 29 546.6 0 29 101 21 546.6 0 21 102 16 546.6 0 16 103 33 546.6 0 33 104 29 546.6 0 29 105 28 546.6 0 28 106 31 546.6 0 31 107 39 546.6 0 39 108 35 546.6 0 35 109 47 546.6 0 47 110 85 546.6 0 85 111 78 546.6 0 78 112 139 546.6 0 139 113 32 546.6 0 32 114 47 546.6 0 47 115 41 546.6 0 41 116 39 546.6 0 39 117 38 546.6 0 38 118 136 546.6 0 136 119 44 546.6 0 44 120 52 546.6 0 52 121 36 546.6 0 36 122 36 546.6 0 36 123 34 546.6 0 34 124 108 546.6 0 108 125 130 546.6 0 130 126 228 546.6 0 228 127 44 546.6 0 44 128 57 546.6 0 57 129 57 546.6 0 57 130 114 546.6 0 114 131 100 546.6 0 100 132 85 546.6 0 85 133 196 546.6 0 196 134 34 546.6 0 34 135 48 546.6 0 48 136 47 546.6 0 47 137 180 546.6 0 180 138 357 546.6 0 357 139 290 546.6 0 290 140 55 546.6 0 55 141 137 546.6 0 137 142 80 546.6 0 80 143 145 546.6 0 145 144 110 546.6 0 110 145 177 546.6 0 177 146 208 546.6 0 208 147 69 546.6 0 69 148 90 546.6 0 90 149 48 546.6 0 48 150 42 546.6 0 42 151 43 546.6 0 43 152 36 546.6 0 36 153 29 546.6 0 29 154 31 546.6 0 31 155 37 546.6 0 37 156 34 546.6 0 34 157 65 546.6 0 65 158 235 546.6 0 235 159 128 546.6 0 128 160 130 546.6 0 130 161 45 546.6 0 45 162 33 546.6 0 33 163 38 546.6 0 38 164 35 546.6 0 35 165 49 546.6 0 49 166 35 546.6 0 35 167 38 546.6 0 38 168 28 546.6 0 28 169 32 546.6 0 32 170 31 546.6 0 31 171 24 546.6 0 24 172 35 546.6 0 35 173 31 546.6 0 31 174 27 546.6 0 27 175 132 546.6 0 132 176 42 546.6 0 42 177 50 546.6 0 50 178 36 546.6 0 36 179 37 546.6 0 37 180 34 546.6 0 34 181 40 546.6 0 40 182 42 546.6 0 42 183 37 546.6 0 37 184 45 546.6 0 45 185 50 546.6 0 50 186 77 546.6 0 77 187 40 546.6 0 40 188 35 546.6 0 35 189 35 546.6 0 35 190 47 546.6 0 47 191 37 546.6 0 37 192 73 546.6 0 73 193 56 546.6 0 56 194 75 546.6 0 75 195 38 546.6 0 38 196 33 546.6 0 33 197 27 546.6 0 27 198 27 546.6 0 27 199 34 546.6 0 34 200 31 546.6 0 31 201 38 546.6 0 38 202 35 546.6 0 35 203 38 546.6 0 38 204 46 546.6 0 46 205 121 546.6 0 121 206 68 546.6 0 68 207 97 546.6 0 97 208 32 546.6 0 32 209 55 546.6 0 55 210 41 546.6 0 41 211 53 546.6 0 53 212 36 546.6 0 36 213 26 546.6 0 26 214 26 546.6 0 26 215 31 546.6 0 31 216 21 546.6 0 21 217 30 546.6 0 30 218 33 546.6 0 33 219 29 546.6 0 29 220 39 546.6 0 39 221 35 546.6 0 35 222 32 546.6 0 32 223 33 546.6 0 33 224 40 546.6 0 40 225 23 546.6 0 23 226 28 546.6 0 28 227 34 546.6 0 34 228 47 546.6 0 47 229 47 546.6 0 47 230 32 546.6 0 32 231 30 546.6 0 30 232 44 546.6 0 44 233 41 546.6 0 41 234 40 546.6 0 40 235 30 546.6 0 30 236 41 546.6 0 41 237 36 546.6 0 36 238 36 546.6 0 36 239 39 546.6 0 39 240 31 546.6 0 31 241 52 546.6 0 52 242 39 546.6 0 39 243 44 546.6 0 44 244 38 546.6 0 38 245 30 546.6 0 30 246 44 546.6 0 44 247 28 546.6 0 28 248 27 546.6 0 27 249 31 546.6 0 31 250 34 546.6 0 34 251 72 546.6 0 72 252 28 546.6 0 28 253 33 546.6 0 33 254 44 546.6 0 44 255 36 546.6 0 36 256 52 546.6 0 52 257 40 546.6 0 40 258 31 546.6 0 31 259 41 546.6 0 41 260 29 546.6 0 29 261 60 546.6 0 60 262 91 546.6 0 91 263 86 546.6 0 86 264 54 546.6 0 54 265 50 546.6 0 50 266 62 546.6 0 62 267 50 546.6 0 50 268 35 546.6 0 35 269 42 546.6 0 42 270 76 546.6 0 76 271 70 546.6 0 70 272 97 546.6 0 97 273 63 546.6 0 63 274 62 546.6 0 62 275 45 546.6 0 45 276 64 546.6 0 64 277 47 546.6 0 47 278 44 546.6 0 44 279 40 546.6 0 40 280 53 546.6 0 53 281 68 546.6 0 68 282 65 546.6 0 65 283 103 546.6 0 103 284 49 546.6 0 49 285 38 546.6 0 38 286 100 546.6 0 100 287 58 546.6 0 58 288 79 546.6 0 79 289 69 546.6 0 69 290 39 546.6 0 39 291 48 546.6 0 48 292 43 546.6 0 43 293 41 546.6 0 41 294 51 546.6 0 51 295 40 546.6 0 40 296 48 546.6 0 48 297 59 546.6 0 59 298 65 546.6 0 65 299 72 546.6 0 72 300 65 546.6 0 65 301 33 546.6 0 33 Finished in 13.39 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 278,887 Reads with adapters: 115,874 (41.5%) Reads that were too short: 202 (0.1%) Reads written (passing filters): 115,672 (41.5%) Total basepairs processed: 79,183,578 bp Total written (filtered): 31,658,820 bp (40.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 115874 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 7 0.0 3 0 0 0 5 2 18 35 0.0 3 0 0 19 12 4 19 1176 0.0 3 0 997 96 64 19 20 113602 0.0 4 107984 4563 782 209 64 21 287 0.0 4 35 220 20 7 5 22 25 0.0 4 2 0 17 2 4 23 191 0.0 4 1 1 1 145 43 24 7 0.0 4 3 0 0 2 2 25 1 0.0 4 1 27 3 0.0 4 0 1 0 0 2 29 1 0.0 4 1 32 1 0.0 4 1 33 1 0.0 4 1 36 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 63 1 0.0 4 1 67 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 93 1 0.0 4 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 285 0.0 4 0 0 0 0 285 128 1 0.0 4 0 0 0 0 1 131 3 0.0 4 0 0 0 0 3 136 4 0.0 4 0 0 0 0 4 137 6 0.0 4 0 0 0 1 5 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 88 0.0 4 0 0 0 3 85 142 32 0.0 4 0 0 0 4 28 143 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 12 0.0 4 0 0 0 0 12 154 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 189 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 207 3 0.0 4 0 0 0 0 3 208 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 212 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 222 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 12 0.0 4 0 0 0 0 12 229 6 0.0 4 0 0 0 0 6 230 1 0.0 4 0 0 0 0 1 233 4 0.0 4 0 0 1 0 3 234 3 0.0 4 0 0 0 1 2 235 4 0.0 4 0 0 0 0 4 237 4 0.0 4 0 0 0 0 4 238 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 280 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 287 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.95 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 115,672 Reads with adapters: 7,147 (6.2%) Reads written (passing filters): 115,672 (100.0%) Total basepairs processed: 31,658,820 bp Total written (filtered): 31,561,422 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7147 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.5% C: 11.9% G: 76.5% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 567 1807.4 0 567 4 2561 451.8 0 2561 5 6 113.0 0 6 6 5 28.2 0 5 7 226 7.1 0 226 8 5 1.8 0 5 9 14 0.4 0 0 14 10 103 0.1 1 7 96 11 1464 0.0 1 79 1385 12 1864 0.0 1 32 1832 13 84 0.0 1 3 81 14 5 0.0 1 0 5 15 14 0.0 1 0 14 16 2 0.0 1 0 2 24 1 0.0 1 1 60 1 0.0 1 1 70 1 0.0 1 0 1 79 2 0.0 1 1 1 93 2 0.0 1 1 1 97 1 0.0 1 1 113 1 0.0 1 1 124 1 0.0 1 1 141 1 0.0 1 1 149 6 0.0 1 5 1 152 47 0.0 1 34 13 153 2 0.0 1 1 1 157 2 0.0 1 2 158 1 0.0 1 1 160 2 0.0 1 0 2 163 1 0.0 1 0 1 179 2 0.0 1 1 1 191 2 0.0 1 0 2 192 1 0.0 1 1 193 1 0.0 1 0 1 198 11 0.0 1 10 1 199 1 0.0 1 0 1 207 1 0.0 1 0 1 210 125 0.0 1 108 17 211 2 0.0 1 2 225 1 0.0 1 1 230 3 0.0 1 3 234 4 0.0 1 4 235 1 0.0 1 1