This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 58.98 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 287,866 (12.9%) Reads that were too short: 2,500 (0.1%) Reads written (passing filters): 285,366 (12.7%) Total basepairs processed: 673,916,425 bp Total written (filtered): 81,593,953 bp (12.1%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 287866 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1611 546.6 0 1611 7 146501 546.6 0 146501 8 1609 546.6 0 1609 9 112666 546.6 0 112666 10 71 546.6 0 71 11 32 546.6 0 32 12 30 546.6 0 30 13 29 546.6 0 29 14 29 546.6 0 29 15 32 546.6 0 32 16 62 546.6 0 62 17 71 546.6 0 71 18 64 546.6 0 64 19 24 546.6 0 24 20 36 546.6 0 36 21 28 546.6 0 28 22 19 546.6 0 19 23 31 546.6 0 31 24 32 546.6 0 32 25 22 546.6 0 22 26 21 546.6 0 21 27 26 546.6 0 26 28 26 546.6 0 26 29 25 546.6 0 25 30 18 546.6 0 18 31 25 546.6 0 25 32 30 546.6 0 30 33 25 546.6 0 25 34 21 546.6 0 21 35 21 546.6 0 21 36 38 546.6 0 38 37 24 546.6 0 24 38 42 546.6 0 42 39 30 546.6 0 30 40 26 546.6 0 26 41 32 546.6 0 32 42 38 546.6 0 38 43 35 546.6 0 35 44 29 546.6 0 29 45 31 546.6 0 31 46 68 546.6 0 68 47 537 546.6 0 537 48 182 546.6 0 182 49 31 546.6 0 31 50 22 546.6 0 22 51 42 546.6 0 42 52 38 546.6 0 38 53 20 546.6 0 20 54 18 546.6 0 18 55 27 546.6 0 27 56 32 546.6 0 32 57 16 546.6 0 16 58 28 546.6 0 28 59 25 546.6 0 25 60 33 546.6 0 33 61 19 546.6 0 19 62 33 546.6 0 33 63 32 546.6 0 32 64 33 546.6 0 33 65 28 546.6 0 28 66 25 546.6 0 25 67 36 546.6 0 36 68 28 546.6 0 28 69 30 546.6 0 30 70 38 546.6 0 38 71 29 546.6 0 29 72 27 546.6 0 27 73 30 546.6 0 30 74 31 546.6 0 31 75 38 546.6 0 38 76 36 546.6 0 36 77 48 546.6 0 48 78 47 546.6 0 47 79 232 546.6 0 232 80 89 546.6 0 89 81 249 546.6 0 249 82 65 546.6 0 65 83 156 546.6 0 156 84 65 546.6 0 65 85 50 546.6 0 50 86 46 546.6 0 46 87 84 546.6 0 84 88 62 546.6 0 62 89 61 546.6 0 61 90 69 546.6 0 69 91 90 546.6 0 90 92 112 546.6 0 112 93 177 546.6 0 177 94 3034 546.6 0 3034 95 5001 546.6 0 5001 96 4640 546.6 0 4640 97 681 546.6 0 681 98 538 546.6 0 538 99 22 546.6 0 22 100 29 546.6 0 29 101 32 546.6 0 32 102 23 546.6 0 23 103 20 546.6 0 20 104 33 546.6 0 33 105 20 546.6 0 20 106 24 546.6 0 24 107 40 546.6 0 40 108 26 546.6 0 26 109 33 546.6 0 33 110 94 546.6 0 94 111 72 546.6 0 72 112 142 546.6 0 142 113 34 546.6 0 34 114 31 546.6 0 31 115 30 546.6 0 30 116 31 546.6 0 31 117 29 546.6 0 29 118 118 546.6 0 118 119 39 546.6 0 39 120 38 546.6 0 38 121 14 546.6 0 14 122 28 546.6 0 28 123 19 546.6 0 19 124 61 546.6 0 61 125 125 546.6 0 125 126 158 546.6 0 158 127 25 546.6 0 25 128 29 546.6 0 29 129 38 546.6 0 38 130 95 546.6 0 95 131 67 546.6 0 67 132 57 546.6 0 57 133 99 546.6 0 99 134 33 546.6 0 33 135 40 546.6 0 40 136 50 546.6 0 50 137 119 546.6 0 119 138 269 546.6 0 269 139 232 546.6 0 232 140 35 546.6 0 35 141 94 546.6 0 94 142 69 546.6 0 69 143 139 546.6 0 139 144 79 546.6 0 79 145 85 546.6 0 85 146 98 546.6 0 98 147 36 546.6 0 36 148 58 546.6 0 58 149 24 546.6 0 24 150 10 546.6 0 10 151 17 546.6 0 17 152 14 546.6 0 14 153 11 546.6 0 11 154 19 546.6 0 19 155 11 546.6 0 11 156 22 546.6 0 22 157 25 546.6 0 25 158 220 546.6 0 220 159 78 546.6 0 78 160 105 546.6 0 105 161 16 546.6 0 16 162 19 546.6 0 19 163 16 546.6 0 16 164 13 546.6 0 13 165 24 546.6 0 24 166 28 546.6 0 28 167 17 546.6 0 17 168 14 546.6 0 14 169 9 546.6 0 9 170 20 546.6 0 20 171 22 546.6 0 22 172 19 546.6 0 19 173 6 546.6 0 6 174 13 546.6 0 13 175 41 546.6 0 41 176 9 546.6 0 9 177 15 546.6 0 15 178 23 546.6 0 23 179 20 546.6 0 20 180 23 546.6 0 23 181 35 546.6 0 35 182 14 546.6 0 14 183 21 546.6 0 21 184 13 546.6 0 13 185 17 546.6 0 17 186 70 546.6 0 70 187 18 546.6 0 18 188 19 546.6 0 19 189 16 546.6 0 16 190 22 546.6 0 22 191 16 546.6 0 16 192 43 546.6 0 43 193 21 546.6 0 21 194 26 546.6 0 26 195 24 546.6 0 24 196 18 546.6 0 18 197 12 546.6 0 12 198 11 546.6 0 11 199 13 546.6 0 13 200 19 546.6 0 19 201 14 546.6 0 14 202 23 546.6 0 23 203 16 546.6 0 16 204 14 546.6 0 14 205 79 546.6 0 79 206 40 546.6 0 40 207 65 546.6 0 65 208 17 546.6 0 17 209 10 546.6 0 10 210 15 546.6 0 15 211 20 546.6 0 20 212 13 546.6 0 13 213 18 546.6 0 18 214 14 546.6 0 14 215 9 546.6 0 9 216 16 546.6 0 16 217 14 546.6 0 14 218 24 546.6 0 24 219 15 546.6 0 15 220 19 546.6 0 19 221 22 546.6 0 22 222 14 546.6 0 14 223 20 546.6 0 20 224 16 546.6 0 16 225 16 546.6 0 16 226 10 546.6 0 10 227 10 546.6 0 10 228 20 546.6 0 20 229 17 546.6 0 17 230 13 546.6 0 13 231 13 546.6 0 13 232 12 546.6 0 12 233 23 546.6 0 23 234 18 546.6 0 18 235 13 546.6 0 13 236 23 546.6 0 23 237 15 546.6 0 15 238 20 546.6 0 20 239 20 546.6 0 20 240 13 546.6 0 13 241 21 546.6 0 21 242 16 546.6 0 16 243 10 546.6 0 10 244 18 546.6 0 18 245 25 546.6 0 25 246 16 546.6 0 16 247 15 546.6 0 15 248 16 546.6 0 16 249 21 546.6 0 21 250 17 546.6 0 17 251 78 546.6 0 78 252 10 546.6 0 10 253 21 546.6 0 21 254 26 546.6 0 26 255 19 546.6 0 19 256 23 546.6 0 23 257 19 546.6 0 19 258 20 546.6 0 20 259 24 546.6 0 24 260 19 546.6 0 19 261 48 546.6 0 48 262 51 546.6 0 51 263 34 546.6 0 34 264 26 546.6 0 26 265 20 546.6 0 20 266 38 546.6 0 38 267 24 546.6 0 24 268 20 546.6 0 20 269 10 546.6 0 10 270 23 546.6 0 23 271 31 546.6 0 31 272 25 546.6 0 25 273 21 546.6 0 21 274 30 546.6 0 30 275 20 546.6 0 20 276 29 546.6 0 29 277 19 546.6 0 19 278 21 546.6 0 21 279 26 546.6 0 26 280 19 546.6 0 19 281 37 546.6 0 37 282 58 546.6 0 58 283 50 546.6 0 50 284 29 546.6 0 29 285 44 546.6 0 44 286 69 546.6 0 69 287 34 546.6 0 34 288 38 546.6 0 38 289 32 546.6 0 32 290 26 546.6 0 26 291 40 546.6 0 40 292 20 546.6 0 20 293 16 546.6 0 16 294 21 546.6 0 21 295 31 546.6 0 31 296 27 546.6 0 27 297 29 546.6 0 29 298 35 546.6 0 35 299 46 546.6 0 46 300 43 546.6 0 43 301 37 546.6 0 37 Finished in 12.12 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 285,366 Reads with adapters: 115,499 (40.5%) Reads that were too short: 58 (0.0%) Reads written (passing filters): 115,441 (40.5%) Total basepairs processed: 81,593,953 bp Total written (filtered): 31,742,454 bp (38.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 115499 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 22 0.0 3 0 0 21 1 16 630 0.0 3 0 614 14 2 17 114341 0.0 3 112122 2142 68 9 18 190 0.0 3 11 168 9 2 19 44 0.0 3 0 0 35 9 20 160 0.0 3 0 0 0 160 21 1 0.0 3 0 0 0 1 25 1 0.0 3 1 30 7 0.0 3 1 0 1 5 33 2 0.0 3 1 0 0 1 34 1 0.0 3 1 45 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 86 1 0.0 3 1 90 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 95 2 0.0 3 0 0 0 2 113 1 0.0 3 0 1 115 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 5 0.0 3 0 0 0 5 145 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 0 7 194 5 0.0 3 0 0 0 5 195 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 12 0.0 3 0 0 0 12 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 1 232 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 279 5 0.0 3 0 0 0 5 280 4 0.0 3 0 0 0 4 281 7 0.0 3 0 0 0 7 282 2 0.0 3 0 0 0 2 283 4 0.0 3 0 0 0 4 286 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.04 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 115,441 Reads with adapters: 2,524 (2.2%) Reads written (passing filters): 115,441 (100.0%) Total basepairs processed: 31,742,454 bp Total written (filtered): 31,734,830 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2524 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 64.9% C: 9.0% G: 23.6% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2473 1803.8 0 2473 4 50 450.9 0 50 5 1 112.7 0 1