This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 136.70 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 431,572 (14.6%) Reads that were too short: 10,687 (0.4%) Reads written (passing filters): 420,885 (14.3%) Total basepairs processed: 887,907,860 bp Total written (filtered): 77,134,649 bp (8.7%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 431572 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 311 720.2 0 311 7 10467 720.2 0 10467 8 329 720.2 0 329 9 11240 720.2 0 11240 10 34 720.2 0 34 11 18 720.2 0 18 12 28 720.2 0 28 13 27 720.2 0 27 14 25 720.2 0 25 15 36 720.2 0 36 16 25 720.2 0 25 17 24 720.2 0 24 18 21 720.2 0 21 19 31 720.2 0 31 20 38 720.2 0 38 21 38 720.2 0 38 22 24 720.2 0 24 23 26 720.2 0 26 24 36 720.2 0 36 25 57 720.2 0 57 26 29 720.2 0 29 27 36 720.2 0 36 28 30 720.2 0 30 29 22 720.2 0 22 30 28 720.2 0 28 31 44 720.2 0 44 32 40 720.2 0 40 33 39 720.2 0 39 34 31 720.2 0 31 35 33 720.2 0 33 36 46 720.2 0 46 37 51 720.2 0 51 38 44 720.2 0 44 39 63 720.2 0 63 40 101 720.2 0 101 41 74 720.2 0 74 42 38 720.2 0 38 43 38 720.2 0 38 44 35 720.2 0 35 45 45 720.2 0 45 46 33 720.2 0 33 47 37 720.2 0 37 48 44 720.2 0 44 49 39 720.2 0 39 50 39 720.2 0 39 51 55 720.2 0 55 52 83 720.2 0 83 53 42 720.2 0 42 54 54 720.2 0 54 55 50 720.2 0 50 56 56 720.2 0 56 57 100 720.2 0 100 58 88 720.2 0 88 59 193 720.2 0 193 60 73 720.2 0 73 61 694 720.2 0 694 62 66 720.2 0 66 63 35 720.2 0 35 64 61 720.2 0 61 65 50 720.2 0 50 66 35 720.2 0 35 67 84 720.2 0 84 68 41 720.2 0 41 69 48 720.2 0 48 70 66 720.2 0 66 71 51 720.2 0 51 72 43 720.2 0 43 73 60 720.2 0 60 74 47 720.2 0 47 75 48 720.2 0 48 76 67 720.2 0 67 77 404 720.2 0 404 78 67 720.2 0 67 79 46 720.2 0 46 80 84 720.2 0 84 81 70 720.2 0 70 82 48 720.2 0 48 83 89 720.2 0 89 84 182 720.2 0 182 85 66 720.2 0 66 86 93 720.2 0 93 87 633 720.2 0 633 88 76 720.2 0 76 89 61 720.2 0 61 90 61 720.2 0 61 91 91 720.2 0 91 92 93 720.2 0 93 93 135 720.2 0 135 94 316 720.2 0 316 95 110 720.2 0 110 96 245 720.2 0 245 97 850 720.2 0 850 98 185 720.2 0 185 99 302 720.2 0 302 100 251 720.2 0 251 101 349 720.2 0 349 102 543 720.2 0 543 103 191 720.2 0 191 104 205 720.2 0 205 105 128 720.2 0 128 106 349 720.2 0 349 107 619 720.2 0 619 108 582 720.2 0 582 109 786 720.2 0 786 110 1158 720.2 0 1158 111 1573 720.2 0 1573 112 3934 720.2 0 3934 113 4079 720.2 0 4079 114 79971 720.2 0 79971 115 52163 720.2 0 52163 116 129931 720.2 0 129931 117 521 720.2 0 521 118 108 720.2 0 108 119 70 720.2 0 70 120 109 720.2 0 109 121 57 720.2 0 57 122 53 720.2 0 53 123 38 720.2 0 38 124 45 720.2 0 45 125 52 720.2 0 52 126 80 720.2 0 80 127 228 720.2 0 228 128 121 720.2 0 121 129 297 720.2 0 297 130 175 720.2 0 175 131 76 720.2 0 76 132 58 720.2 0 58 133 54 720.2 0 54 134 73 720.2 0 73 135 121 720.2 0 121 136 87 720.2 0 87 137 74 720.2 0 74 138 65 720.2 0 65 139 94 720.2 0 94 140 228 720.2 0 228 141 451 720.2 0 451 142 599 720.2 0 599 143 696 720.2 0 696 144 559 720.2 0 559 145 787 720.2 0 787 146 964 720.2 0 964 147 14794 720.2 0 14794 148 21964 720.2 0 21964 149 50259 720.2 0 50259 150 507 720.2 0 507 151 213 720.2 0 213 152 298 720.2 0 298 153 5250 720.2 0 5250 154 2867 720.2 0 2867 155 6074 720.2 0 6074 156 260 720.2 0 260 157 310 720.2 0 310 158 133 720.2 0 133 159 52 720.2 0 52 160 72 720.2 0 72 161 113 720.2 0 113 162 107 720.2 0 107 163 139 720.2 0 139 164 52 720.2 0 52 165 66 720.2 0 66 166 39 720.2 0 39 167 42 720.2 0 42 168 108 720.2 0 108 169 91 720.2 0 91 170 124 720.2 0 124 171 289 720.2 0 289 172 40 720.2 0 40 173 20 720.2 0 20 174 31 720.2 0 31 175 40 720.2 0 40 176 49 720.2 0 49 177 44 720.2 0 44 178 32 720.2 0 32 179 25 720.2 0 25 180 43 720.2 0 43 181 149 720.2 0 149 182 100 720.2 0 100 183 352 720.2 0 352 184 172 720.2 0 172 185 324 720.2 0 324 186 341 720.2 0 341 187 39 720.2 0 39 188 48 720.2 0 48 189 38 720.2 0 38 190 47 720.2 0 47 191 80 720.2 0 80 192 41 720.2 0 41 193 59 720.2 0 59 194 34 720.2 0 34 195 33 720.2 0 33 196 35 720.2 0 35 197 53 720.2 0 53 198 59 720.2 0 59 199 37 720.2 0 37 200 27 720.2 0 27 201 27 720.2 0 27 202 65 720.2 0 65 203 46 720.2 0 46 204 96 720.2 0 96 205 27 720.2 0 27 206 50 720.2 0 50 207 37 720.2 0 37 208 23 720.2 0 23 209 33 720.2 0 33 210 17 720.2 0 17 211 28 720.2 0 28 212 25 720.2 0 25 213 32 720.2 0 32 214 30 720.2 0 30 215 32 720.2 0 32 216 191 720.2 0 191 217 99 720.2 0 99 218 246 720.2 0 246 219 34 720.2 0 34 220 40 720.2 0 40 221 30 720.2 0 30 222 36 720.2 0 36 223 29 720.2 0 29 224 31 720.2 0 31 225 29 720.2 0 29 226 63 720.2 0 63 227 61 720.2 0 61 228 166 720.2 0 166 229 21 720.2 0 21 230 48 720.2 0 48 231 29 720.2 0 29 232 28 720.2 0 28 233 42 720.2 0 42 234 71 720.2 0 71 235 111 720.2 0 111 236 169 720.2 0 169 237 122 720.2 0 122 238 147 720.2 0 147 239 53 720.2 0 53 240 32 720.2 0 32 241 24 720.2 0 24 242 36 720.2 0 36 243 26 720.2 0 26 244 32 720.2 0 32 245 41 720.2 0 41 246 50 720.2 0 50 247 33 720.2 0 33 248 47 720.2 0 47 249 45 720.2 0 45 250 66 720.2 0 66 251 52 720.2 0 52 252 31 720.2 0 31 253 61 720.2 0 61 254 46 720.2 0 46 255 32 720.2 0 32 256 28 720.2 0 28 257 64 720.2 0 64 258 43 720.2 0 43 259 58 720.2 0 58 260 46 720.2 0 46 261 56 720.2 0 56 262 53 720.2 0 53 263 37 720.2 0 37 264 42 720.2 0 42 265 44 720.2 0 44 266 53 720.2 0 53 267 56 720.2 0 56 268 36 720.2 0 36 269 68 720.2 0 68 270 82 720.2 0 82 271 107 720.2 0 107 272 108 720.2 0 108 273 70 720.2 0 70 274 75 720.2 0 75 275 80 720.2 0 80 276 88 720.2 0 88 277 91 720.2 0 91 278 98 720.2 0 98 279 759 720.2 0 759 280 466 720.2 0 466 281 1123 720.2 0 1123 282 838 720.2 0 838 283 793 720.2 0 793 284 86 720.2 0 86 285 114 720.2 0 114 286 142 720.2 0 142 287 67 720.2 0 67 288 59 720.2 0 59 289 76 720.2 0 76 290 219 720.2 0 219 291 282 720.2 0 282 292 303 720.2 0 303 293 129 720.2 0 129 294 87 720.2 0 87 295 139 720.2 0 139 296 88 720.2 0 88 297 97 720.2 0 97 298 126 720.2 0 126 299 42 720.2 0 42 300 43 720.2 0 43 301 35 720.2 0 35 Finished in 21.63 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 420,885 Reads with adapters: 10,512 (2.5%) Reads that were too short: 59 (0.0%) Reads written (passing filters): 10,453 (2.5%) Total basepairs processed: 77,134,649 bp Total written (filtered): 2,881,891 bp (3.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10512 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 58 0.0 3 0 55 1 2 17 10268 0.0 3 10071 184 13 18 20 0.0 3 2 17 1 19 3 0.0 3 0 0 3 20 14 0.0 3 0 0 0 14 21 1 0.0 3 0 0 0 1 22 3 0.0 3 0 0 0 3 23 1 0.0 3 0 1 24 1 0.0 3 1 27 3 0.0 3 1 0 0 2 31 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 45 4 0.0 3 3 0 1 47 2 0.0 3 0 1 0 1 49 1 0.0 3 0 0 0 1 51 10 0.0 3 0 0 0 10 54 1 0.0 3 0 0 0 1 58 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 1 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 33 0.0 3 0 0 0 33 86 3 0.0 3 0 0 0 3 88 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 1 93 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 1 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 124 1 0.0 3 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 146 1 0.0 3 1 148 3 0.0 3 0 0 0 3 152 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 4 0.0 3 0 0 0 4 173 3 0.0 3 0 0 0 3 174 7 0.0 3 0 0 0 7 175 7 0.0 3 0 0 0 7 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 203 1 0.0 3 1 209 1 0.0 3 1 223 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 249 4 0.0 3 0 0 0 4 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.56 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 10,453 Reads with adapters: 58 (0.6%) Reads written (passing filters): 10,453 (100.0%) Total basepairs processed: 2,881,891 bp Total written (filtered): 2,881,705 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 58 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.1% C: 24.1% G: 22.4% T: 10.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 49 163.3 0 49 4 7 40.8 0 7 5 1 10.2 0 1 6 1 2.6 0 1