This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 132.19 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 289,385 (9.8%) Reads that were too short: 98,988 (3.4%) Reads written (passing filters): 190,397 (6.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 54,152,719 bp (6.1%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 289385 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3031 720.2 0 3031 7 71448 720.2 0 71448 8 2134 720.2 0 2134 9 98326 720.2 0 98326 10 102 720.2 0 102 11 41 720.2 0 41 12 183 720.2 0 183 13 24 720.2 0 24 14 29 720.2 0 29 15 35 720.2 0 35 16 26 720.2 0 26 17 112 720.2 0 112 18 32 720.2 0 32 19 57 720.2 0 57 20 40 720.2 0 40 21 29 720.2 0 29 22 35 720.2 0 35 23 35 720.2 0 35 24 27 720.2 0 27 25 32 720.2 0 32 26 33 720.2 0 33 27 43 720.2 0 43 28 40 720.2 0 40 29 34 720.2 0 34 30 49 720.2 0 49 31 61 720.2 0 61 32 46 720.2 0 46 33 49 720.2 0 49 34 33 720.2 0 33 35 27 720.2 0 27 36 33 720.2 0 33 37 42 720.2 0 42 38 62 720.2 0 62 39 35 720.2 0 35 40 46 720.2 0 46 41 40 720.2 0 40 42 39 720.2 0 39 43 26 720.2 0 26 44 30 720.2 0 30 45 37 720.2 0 37 46 39 720.2 0 39 47 49 720.2 0 49 48 35 720.2 0 35 49 66 720.2 0 66 50 37 720.2 0 37 51 63 720.2 0 63 52 53 720.2 0 53 53 148 720.2 0 148 54 85 720.2 0 85 55 64 720.2 0 64 56 39 720.2 0 39 57 391 720.2 0 391 58 51 720.2 0 51 59 51 720.2 0 51 60 62 720.2 0 62 61 47 720.2 0 47 62 39 720.2 0 39 63 53 720.2 0 53 64 67 720.2 0 67 65 71 720.2 0 71 66 35 720.2 0 35 67 47 720.2 0 47 68 69 720.2 0 69 69 61 720.2 0 61 70 51 720.2 0 51 71 361 720.2 0 361 72 148 720.2 0 148 73 167 720.2 0 167 74 37 720.2 0 37 75 38 720.2 0 38 76 29 720.2 0 29 77 39 720.2 0 39 78 42 720.2 0 42 79 48 720.2 0 48 80 35 720.2 0 35 81 57 720.2 0 57 82 55 720.2 0 55 83 38 720.2 0 38 84 45 720.2 0 45 85 53 720.2 0 53 86 43 720.2 0 43 87 28 720.2 0 28 88 21 720.2 0 21 89 49 720.2 0 49 90 28 720.2 0 28 91 60 720.2 0 60 92 123 720.2 0 123 93 67 720.2 0 67 94 56 720.2 0 56 95 66 720.2 0 66 96 64 720.2 0 64 97 44 720.2 0 44 98 58 720.2 0 58 99 45 720.2 0 45 100 38 720.2 0 38 101 35 720.2 0 35 102 116 720.2 0 116 103 50 720.2 0 50 104 31 720.2 0 31 105 34 720.2 0 34 106 38 720.2 0 38 107 30 720.2 0 30 108 32 720.2 0 32 109 47 720.2 0 47 110 40 720.2 0 40 111 43 720.2 0 43 112 28 720.2 0 28 113 33 720.2 0 33 114 53 720.2 0 53 115 41 720.2 0 41 116 42 720.2 0 42 117 74 720.2 0 74 118 44 720.2 0 44 119 34 720.2 0 34 120 105 720.2 0 105 121 64 720.2 0 64 122 69 720.2 0 69 123 1032 720.2 0 1032 124 82 720.2 0 82 125 39 720.2 0 39 126 59 720.2 0 59 127 44 720.2 0 44 128 72 720.2 0 72 129 97 720.2 0 97 130 1381 720.2 0 1381 131 639 720.2 0 639 132 349 720.2 0 349 133 240 720.2 0 240 134 99 720.2 0 99 135 39 720.2 0 39 136 81 720.2 0 81 137 408 720.2 0 408 138 724 720.2 0 724 139 248 720.2 0 248 140 49 720.2 0 49 141 53 720.2 0 53 142 62 720.2 0 62 143 36 720.2 0 36 144 46 720.2 0 46 145 39 720.2 0 39 146 39 720.2 0 39 147 26 720.2 0 26 148 66 720.2 0 66 149 32 720.2 0 32 150 54 720.2 0 54 151 176 720.2 0 176 152 246 720.2 0 246 153 95 720.2 0 95 154 36 720.2 0 36 155 49 720.2 0 49 156 22 720.2 0 22 157 27 720.2 0 27 158 43 720.2 0 43 159 39 720.2 0 39 160 30 720.2 0 30 161 22 720.2 0 22 162 26 720.2 0 26 163 46 720.2 0 46 164 79 720.2 0 79 165 52 720.2 0 52 166 30 720.2 0 30 167 45 720.2 0 45 168 27 720.2 0 27 169 26 720.2 0 26 170 28 720.2 0 28 171 22 720.2 0 22 172 25 720.2 0 25 173 23 720.2 0 23 174 23 720.2 0 23 175 40 720.2 0 40 176 30 720.2 0 30 177 81 720.2 0 81 178 113 720.2 0 113 179 51 720.2 0 51 180 35 720.2 0 35 181 46 720.2 0 46 182 45 720.2 0 45 183 53 720.2 0 53 184 43 720.2 0 43 185 40 720.2 0 40 186 90 720.2 0 90 187 129 720.2 0 129 188 126 720.2 0 126 189 105 720.2 0 105 190 63 720.2 0 63 191 50 720.2 0 50 192 29 720.2 0 29 193 39 720.2 0 39 194 54 720.2 0 54 195 37 720.2 0 37 196 56 720.2 0 56 197 64 720.2 0 64 198 41 720.2 0 41 199 59 720.2 0 59 200 52 720.2 0 52 201 33 720.2 0 33 202 47 720.2 0 47 203 54 720.2 0 54 204 63 720.2 0 63 205 94 720.2 0 94 206 71 720.2 0 71 207 41 720.2 0 41 208 47 720.2 0 47 209 38 720.2 0 38 210 60 720.2 0 60 211 75 720.2 0 75 212 476 720.2 0 476 213 743 720.2 0 743 214 403 720.2 0 403 215 61 720.2 0 61 216 54 720.2 0 54 217 114 720.2 0 114 218 107 720.2 0 107 219 51 720.2 0 51 220 64 720.2 0 64 221 51 720.2 0 51 222 51 720.2 0 51 223 42 720.2 0 42 224 56 720.2 0 56 225 55 720.2 0 55 226 45 720.2 0 45 227 35 720.2 0 35 228 61 720.2 0 61 229 44 720.2 0 44 230 51 720.2 0 51 231 60 720.2 0 60 232 72 720.2 0 72 233 148 720.2 0 148 234 255 720.2 0 255 235 183 720.2 0 183 236 568 720.2 0 568 237 359 720.2 0 359 238 360 720.2 0 360 239 122 720.2 0 122 240 65 720.2 0 65 241 95 720.2 0 95 242 175 720.2 0 175 243 427 720.2 0 427 244 818 720.2 0 818 245 716 720.2 0 716 246 397 720.2 0 397 247 211 720.2 0 211 248 150 720.2 0 150 249 92 720.2 0 92 250 105 720.2 0 105 251 101 720.2 0 101 252 106 720.2 0 106 253 77 720.2 0 77 254 88 720.2 0 88 255 205 720.2 0 205 256 211 720.2 0 211 257 372 720.2 0 372 258 316 720.2 0 316 259 266 720.2 0 266 260 726 720.2 0 726 261 1373 720.2 0 1373 262 739 720.2 0 739 263 209 720.2 0 209 264 157 720.2 0 157 265 174 720.2 0 174 266 316 720.2 0 316 267 250 720.2 0 250 268 137 720.2 0 137 269 110 720.2 0 110 270 137 720.2 0 137 271 205 720.2 0 205 272 294 720.2 0 294 273 538 720.2 0 538 274 216 720.2 0 216 275 423 720.2 0 423 276 385 720.2 0 385 277 256 720.2 0 256 278 278 720.2 0 278 279 345 720.2 0 345 280 404 720.2 0 404 281 749 720.2 0 749 282 529 720.2 0 529 283 629 720.2 0 629 284 853 720.2 0 853 285 1252 720.2 0 1252 286 19698 720.2 0 19698 287 39266 720.2 0 39266 288 15576 720.2 0 15576 289 119 720.2 0 119 290 83 720.2 0 83 291 121 720.2 0 121 292 143 720.2 0 143 293 93 720.2 0 93 294 92 720.2 0 92 295 234 720.2 0 234 296 163 720.2 0 163 297 385 720.2 0 385 298 464 720.2 0 464 299 436 720.2 0 436 300 107 720.2 0 107 301 50 720.2 0 50 Finished in 14.29 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 190,397 Reads with adapters: 73,861 (38.8%) Reads that were too short: 69 (0.0%) Reads written (passing filters): 73,792 (38.8%) Total basepairs processed: 54,152,719 bp Total written (filtered): 20,096,086 bp (37.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 73861 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 5 4 18 24 0.0 3 0 0 19 4 1 19 791 0.0 3 0 681 54 37 19 20 71485 0.0 4 67095 3543 647 160 40 21 164 0.0 4 22 128 9 3 2 22 21 0.0 4 0 0 8 5 8 23 146 0.0 4 1 0 0 103 42 24 3 0.0 4 0 0 0 1 2 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 55 3 0.0 4 0 0 0 0 3 57 1 0.0 4 0 1 59 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 99 2 0.0 4 0 0 0 0 2 114 2 0.0 4 1 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 1 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 1 122 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 18 0.0 4 0 0 0 0 18 127 1097 0.0 4 0 0 0 0 1097 128 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 6 0.0 4 0 0 0 0 6 158 1 0.0 4 0 0 0 0 1 163 3 0.0 4 0 0 0 0 3 167 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 1 197 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 3 0.0 4 0 0 0 0 3 225 2 0.0 4 1 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 15 0.0 4 0 0 0 0 15 229 7 0.0 4 0 0 0 0 7 232 4 0.0 4 0 0 0 0 4 233 2 0.0 4 0 0 0 0 2 234 4 0.0 4 0 0 0 1 3 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 0 1 269 2 0.0 4 0 0 0 0 2 279 1 0.0 4 0 0 0 0 1 281 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.91 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 73,792 Reads with adapters: 3,188 (4.3%) Reads written (passing filters): 73,792 (100.0%) Total basepairs processed: 20,096,086 bp Total written (filtered): 20,051,669 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3188 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.0% C: 11.2% G: 77.5% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 276 1153.0 0 276 4 54 288.2 0 54 5 4 72.1 0 4 9 18 0.3 0 6 12 10 79 0.1 1 2 77 11 2118 0.0 1 43 2075 12 544 0.0 1 8 536 13 14 0.0 1 1 13 15 9 0.0 1 0 9 16 1 0.0 1 0 1 20 3 0.0 1 2 1 21 1 0.0 1 1 24 2 0.0 1 2 67 1 0.0 1 1 74 1 0.0 1 1 94 1 0.0 1 1 109 1 0.0 1 1 113 1 0.0 1 1 149 1 0.0 1 1 152 11 0.0 1 7 4 155 1 0.0 1 1 159 2 0.0 1 1 1 171 1 0.0 1 1 179 1 0.0 1 1 191 1 0.0 1 0 1 210 41 0.0 1 34 7 211 1 0.0 1 1