This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 129.58 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 303,470 (10.3%) Reads that were too short: 110,272 (3.7%) Reads written (passing filters): 193,198 (6.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 55,306,218 bp (6.2%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 303470 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3144 720.2 0 3144 7 100622 720.2 0 100622 8 1437 720.2 0 1437 9 74324 720.2 0 74324 10 76 720.2 0 76 11 45 720.2 0 45 12 125 720.2 0 125 13 32 720.2 0 32 14 36 720.2 0 36 15 44 720.2 0 44 16 35 720.2 0 35 17 48 720.2 0 48 18 43 720.2 0 43 19 35 720.2 0 35 20 53 720.2 0 53 21 39 720.2 0 39 22 32 720.2 0 32 23 37 720.2 0 37 24 41 720.2 0 41 25 36 720.2 0 36 26 62 720.2 0 62 27 42 720.2 0 42 28 44 720.2 0 44 29 33 720.2 0 33 30 44 720.2 0 44 31 29 720.2 0 29 32 55 720.2 0 55 33 48 720.2 0 48 34 41 720.2 0 41 35 48 720.2 0 48 36 58 720.2 0 58 37 49 720.2 0 49 38 61 720.2 0 61 39 39 720.2 0 39 40 64 720.2 0 64 41 54 720.2 0 54 42 57 720.2 0 57 43 41 720.2 0 41 44 46 720.2 0 46 45 46 720.2 0 46 46 33 720.2 0 33 47 45 720.2 0 45 48 37 720.2 0 37 49 62 720.2 0 62 50 40 720.2 0 40 51 59 720.2 0 59 52 66 720.2 0 66 53 296 720.2 0 296 54 103 720.2 0 103 55 64 720.2 0 64 56 49 720.2 0 49 57 412 720.2 0 412 58 67 720.2 0 67 59 45 720.2 0 45 60 45 720.2 0 45 61 43 720.2 0 43 62 48 720.2 0 48 63 43 720.2 0 43 64 62 720.2 0 62 65 68 720.2 0 68 66 44 720.2 0 44 67 55 720.2 0 55 68 65 720.2 0 65 69 69 720.2 0 69 70 47 720.2 0 47 71 424 720.2 0 424 72 144 720.2 0 144 73 206 720.2 0 206 74 34 720.2 0 34 75 42 720.2 0 42 76 32 720.2 0 32 77 49 720.2 0 49 78 32 720.2 0 32 79 28 720.2 0 28 80 31 720.2 0 31 81 38 720.2 0 38 82 41 720.2 0 41 83 34 720.2 0 34 84 44 720.2 0 44 85 33 720.2 0 33 86 38 720.2 0 38 87 23 720.2 0 23 88 23 720.2 0 23 89 28 720.2 0 28 90 39 720.2 0 39 91 49 720.2 0 49 92 157 720.2 0 157 93 57 720.2 0 57 94 49 720.2 0 49 95 54 720.2 0 54 96 66 720.2 0 66 97 47 720.2 0 47 98 50 720.2 0 50 99 37 720.2 0 37 100 37 720.2 0 37 101 27 720.2 0 27 102 68 720.2 0 68 103 43 720.2 0 43 104 29 720.2 0 29 105 31 720.2 0 31 106 36 720.2 0 36 107 28 720.2 0 28 108 33 720.2 0 33 109 32 720.2 0 32 110 37 720.2 0 37 111 45 720.2 0 45 112 33 720.2 0 33 113 35 720.2 0 35 114 34 720.2 0 34 115 31 720.2 0 31 116 28 720.2 0 28 117 62 720.2 0 62 118 47 720.2 0 47 119 24 720.2 0 24 120 94 720.2 0 94 121 55 720.2 0 55 122 48 720.2 0 48 123 1014 720.2 0 1014 124 70 720.2 0 70 125 28 720.2 0 28 126 59 720.2 0 59 127 36 720.2 0 36 128 61 720.2 0 61 129 78 720.2 0 78 130 1365 720.2 0 1365 131 474 720.2 0 474 132 207 720.2 0 207 133 196 720.2 0 196 134 58 720.2 0 58 135 25 720.2 0 25 136 40 720.2 0 40 137 305 720.2 0 305 138 596 720.2 0 596 139 210 720.2 0 210 140 32 720.2 0 32 141 21 720.2 0 21 142 30 720.2 0 30 143 26 720.2 0 26 144 20 720.2 0 20 145 26 720.2 0 26 146 39 720.2 0 39 147 20 720.2 0 20 148 44 720.2 0 44 149 29 720.2 0 29 150 40 720.2 0 40 151 71 720.2 0 71 152 145 720.2 0 145 153 70 720.2 0 70 154 37 720.2 0 37 155 33 720.2 0 33 156 23 720.2 0 23 157 26 720.2 0 26 158 34 720.2 0 34 159 36 720.2 0 36 160 21 720.2 0 21 161 29 720.2 0 29 162 10 720.2 0 10 163 32 720.2 0 32 164 45 720.2 0 45 165 32 720.2 0 32 166 19 720.2 0 19 167 32 720.2 0 32 168 33 720.2 0 33 169 20 720.2 0 20 170 30 720.2 0 30 171 17 720.2 0 17 172 22 720.2 0 22 173 28 720.2 0 28 174 25 720.2 0 25 175 21 720.2 0 21 176 34 720.2 0 34 177 101 720.2 0 101 178 110 720.2 0 110 179 49 720.2 0 49 180 27 720.2 0 27 181 33 720.2 0 33 182 25 720.2 0 25 183 32 720.2 0 32 184 40 720.2 0 40 185 17 720.2 0 17 186 63 720.2 0 63 187 64 720.2 0 64 188 62 720.2 0 62 189 51 720.2 0 51 190 42 720.2 0 42 191 41 720.2 0 41 192 27 720.2 0 27 193 31 720.2 0 31 194 27 720.2 0 27 195 25 720.2 0 25 196 29 720.2 0 29 197 37 720.2 0 37 198 27 720.2 0 27 199 29 720.2 0 29 200 34 720.2 0 34 201 23 720.2 0 23 202 18 720.2 0 18 203 33 720.2 0 33 204 34 720.2 0 34 205 58 720.2 0 58 206 31 720.2 0 31 207 20 720.2 0 20 208 20 720.2 0 20 209 18 720.2 0 18 210 26 720.2 0 26 211 36 720.2 0 36 212 185 720.2 0 185 213 418 720.2 0 418 214 112 720.2 0 112 215 34 720.2 0 34 216 34 720.2 0 34 217 60 720.2 0 60 218 66 720.2 0 66 219 39 720.2 0 39 220 69 720.2 0 69 221 33 720.2 0 33 222 25 720.2 0 25 223 34 720.2 0 34 224 20 720.2 0 20 225 41 720.2 0 41 226 30 720.2 0 30 227 30 720.2 0 30 228 38 720.2 0 38 229 33 720.2 0 33 230 35 720.2 0 35 231 32 720.2 0 32 232 40 720.2 0 40 233 66 720.2 0 66 234 98 720.2 0 98 235 162 720.2 0 162 236 585 720.2 0 585 237 143 720.2 0 143 238 98 720.2 0 98 239 55 720.2 0 55 240 21 720.2 0 21 241 40 720.2 0 40 242 56 720.2 0 56 243 120 720.2 0 120 244 352 720.2 0 352 245 339 720.2 0 339 246 188 720.2 0 188 247 126 720.2 0 126 248 83 720.2 0 83 249 49 720.2 0 49 250 52 720.2 0 52 251 50 720.2 0 50 252 38 720.2 0 38 253 46 720.2 0 46 254 60 720.2 0 60 255 117 720.2 0 117 256 132 720.2 0 132 257 213 720.2 0 213 258 134 720.2 0 134 259 139 720.2 0 139 260 674 720.2 0 674 261 1384 720.2 0 1384 262 719 720.2 0 719 263 196 720.2 0 196 264 120 720.2 0 120 265 143 720.2 0 143 266 207 720.2 0 207 267 164 720.2 0 164 268 69 720.2 0 69 269 82 720.2 0 82 270 134 720.2 0 134 271 160 720.2 0 160 272 315 720.2 0 315 273 605 720.2 0 605 274 236 720.2 0 236 275 500 720.2 0 500 276 413 720.2 0 413 277 230 720.2 0 230 278 187 720.2 0 187 279 441 720.2 0 441 280 435 720.2 0 435 281 735 720.2 0 735 282 531 720.2 0 531 283 625 720.2 0 625 284 778 720.2 0 778 285 1064 720.2 0 1064 286 23037 720.2 0 23037 287 49829 720.2 0 49829 288 19694 720.2 0 19694 289 92 720.2 0 92 290 40 720.2 0 40 291 52 720.2 0 52 292 73 720.2 0 73 293 40 720.2 0 40 294 38 720.2 0 38 295 192 720.2 0 192 296 75 720.2 0 75 297 176 720.2 0 176 298 225 720.2 0 225 299 221 720.2 0 221 300 47 720.2 0 47 301 30 720.2 0 30 Finished in 13.27 s (69 us/read; 0.87 M reads/minute). === Summary === Total reads processed: 193,198 Reads with adapters: 76,496 (39.6%) Reads that were too short: 153 (0.1%) Reads written (passing filters): 76,343 (39.5%) Total basepairs processed: 55,306,218 bp Total written (filtered): 20,989,010 bp (38.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 76496 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 14 0.0 3 0 0 11 3 16 425 0.0 3 0 415 9 1 17 75548 0.0 3 73597 1878 67 6 18 156 0.0 3 7 143 5 1 19 26 0.0 3 0 0 20 6 20 111 0.0 3 1 0 1 109 23 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 0 5 50 2 0.0 3 0 0 0 2 55 1 0.0 3 0 1 61 8 0.0 3 0 0 0 8 63 4 0.0 3 0 0 0 4 64 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 2 0.0 3 2 98 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 51 0.0 3 0 0 1 50 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 54 0.0 3 0 0 1 53 173 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 192 12 0.0 3 0 0 0 12 194 3 0.0 3 0 0 1 2 200 1 0.0 3 0 0 0 1 207 15 0.0 3 0 0 0 15 210 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.27 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 76,343 Reads with adapters: 3,462 (4.5%) Reads written (passing filters): 76,343 (100.0%) Total basepairs processed: 20,989,010 bp Total written (filtered): 20,978,571 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3462 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.9% C: 5.1% G: 16.2% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3415 1192.9 0 3415 4 46 298.2 0 46 10 1 0.1 1 0 1