This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 130.82 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 185,007 (6.3%) Reads that were too short: 20,818 (0.7%) Reads written (passing filters): 164,189 (5.6%) Total basepairs processed: 887,907,860 bp Total written (filtered): 45,431,794 bp (5.1%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 185007 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 944 720.2 0 944 7 3350 720.2 0 3350 8 84808 720.2 0 84808 9 52981 720.2 0 52981 10 109 720.2 0 109 11 44 720.2 0 44 12 45 720.2 0 45 13 54 720.2 0 54 14 50 720.2 0 50 15 41 720.2 0 41 16 61 720.2 0 61 17 40 720.2 0 40 18 34 720.2 0 34 19 41 720.2 0 41 20 56 720.2 0 56 21 59 720.2 0 59 22 49 720.2 0 49 23 52 720.2 0 52 24 51 720.2 0 51 25 51 720.2 0 51 26 42 720.2 0 42 27 40 720.2 0 40 28 50 720.2 0 50 29 37 720.2 0 37 30 46 720.2 0 46 31 39 720.2 0 39 32 48 720.2 0 48 33 64 720.2 0 64 34 42 720.2 0 42 35 53 720.2 0 53 36 72 720.2 0 72 37 73 720.2 0 73 38 80 720.2 0 80 39 52 720.2 0 52 40 104 720.2 0 104 41 63 720.2 0 63 42 59 720.2 0 59 43 36 720.2 0 36 44 34 720.2 0 34 45 35 720.2 0 35 46 52 720.2 0 52 47 44 720.2 0 44 48 36 720.2 0 36 49 49 720.2 0 49 50 44 720.2 0 44 51 78 720.2 0 78 52 63 720.2 0 63 53 65 720.2 0 65 54 66 720.2 0 66 55 52 720.2 0 52 56 42 720.2 0 42 57 46 720.2 0 46 58 69 720.2 0 69 59 45 720.2 0 45 60 73 720.2 0 73 61 69 720.2 0 69 62 50 720.2 0 50 63 52 720.2 0 52 64 47 720.2 0 47 65 53 720.2 0 53 66 71 720.2 0 71 67 70 720.2 0 70 68 99 720.2 0 99 69 74 720.2 0 74 70 58 720.2 0 58 71 48 720.2 0 48 72 61 720.2 0 61 73 59 720.2 0 59 74 82 720.2 0 82 75 73 720.2 0 73 76 66 720.2 0 66 77 58 720.2 0 58 78 103 720.2 0 103 79 70 720.2 0 70 80 109 720.2 0 109 81 63 720.2 0 63 82 104 720.2 0 104 83 45 720.2 0 45 84 71 720.2 0 71 85 56 720.2 0 56 86 48 720.2 0 48 87 81 720.2 0 81 88 74 720.2 0 74 89 53 720.2 0 53 90 61 720.2 0 61 91 138 720.2 0 138 92 76 720.2 0 76 93 115 720.2 0 115 94 116 720.2 0 116 95 132 720.2 0 132 96 82 720.2 0 82 97 621 720.2 0 621 98 659 720.2 0 659 99 999 720.2 0 999 100 418 720.2 0 418 101 44 720.2 0 44 102 39 720.2 0 39 103 52 720.2 0 52 104 59 720.2 0 59 105 54 720.2 0 54 106 63 720.2 0 63 107 89 720.2 0 89 108 124 720.2 0 124 109 213 720.2 0 213 110 97 720.2 0 97 111 404 720.2 0 404 112 389 720.2 0 389 113 735 720.2 0 735 114 67 720.2 0 67 115 44 720.2 0 44 116 55 720.2 0 55 117 34 720.2 0 34 118 43 720.2 0 43 119 55 720.2 0 55 120 55 720.2 0 55 121 54 720.2 0 54 122 54 720.2 0 54 123 69 720.2 0 69 124 61 720.2 0 61 125 58 720.2 0 58 126 72 720.2 0 72 127 55 720.2 0 55 128 54 720.2 0 54 129 80 720.2 0 80 130 78 720.2 0 78 131 97 720.2 0 97 132 128 720.2 0 128 133 127 720.2 0 127 134 239 720.2 0 239 135 115 720.2 0 115 136 79 720.2 0 79 137 61 720.2 0 61 138 62 720.2 0 62 139 65 720.2 0 65 140 90 720.2 0 90 141 157 720.2 0 157 142 109 720.2 0 109 143 66 720.2 0 66 144 75 720.2 0 75 145 75 720.2 0 75 146 132 720.2 0 132 147 98 720.2 0 98 148 112 720.2 0 112 149 66 720.2 0 66 150 59 720.2 0 59 151 61 720.2 0 61 152 64 720.2 0 64 153 77 720.2 0 77 154 189 720.2 0 189 155 255 720.2 0 255 156 476 720.2 0 476 157 294 720.2 0 294 158 64 720.2 0 64 159 66 720.2 0 66 160 59 720.2 0 59 161 67 720.2 0 67 162 54 720.2 0 54 163 48 720.2 0 48 164 75 720.2 0 75 165 57 720.2 0 57 166 259 720.2 0 259 167 329 720.2 0 329 168 338 720.2 0 338 169 61 720.2 0 61 170 42 720.2 0 42 171 48 720.2 0 48 172 59 720.2 0 59 173 47 720.2 0 47 174 62 720.2 0 62 175 52 720.2 0 52 176 63 720.2 0 63 177 81 720.2 0 81 178 58 720.2 0 58 179 60 720.2 0 60 180 72 720.2 0 72 181 74 720.2 0 74 182 77 720.2 0 77 183 64 720.2 0 64 184 45 720.2 0 45 185 64 720.2 0 64 186 74 720.2 0 74 187 78 720.2 0 78 188 80 720.2 0 80 189 72 720.2 0 72 190 92 720.2 0 92 191 85 720.2 0 85 192 117 720.2 0 117 193 260 720.2 0 260 194 1482 720.2 0 1482 195 659 720.2 0 659 196 1051 720.2 0 1051 197 128 720.2 0 128 198 163 720.2 0 163 199 100 720.2 0 100 200 158 720.2 0 158 201 170 720.2 0 170 202 138 720.2 0 138 203 58 720.2 0 58 204 66 720.2 0 66 205 77 720.2 0 77 206 81 720.2 0 81 207 76 720.2 0 76 208 87 720.2 0 87 209 73 720.2 0 73 210 77 720.2 0 77 211 74 720.2 0 74 212 66 720.2 0 66 213 66 720.2 0 66 214 69 720.2 0 69 215 119 720.2 0 119 216 141 720.2 0 141 217 130 720.2 0 130 218 77 720.2 0 77 219 74 720.2 0 74 220 314 720.2 0 314 221 440 720.2 0 440 222 485 720.2 0 485 223 331 720.2 0 331 224 69 720.2 0 69 225 66 720.2 0 66 226 70 720.2 0 70 227 64 720.2 0 64 228 80 720.2 0 80 229 108 720.2 0 108 230 63 720.2 0 63 231 48 720.2 0 48 232 58 720.2 0 58 233 74 720.2 0 74 234 53 720.2 0 53 235 68 720.2 0 68 236 95 720.2 0 95 237 246 720.2 0 246 238 372 720.2 0 372 239 379 720.2 0 379 240 139 720.2 0 139 241 185 720.2 0 185 242 241 720.2 0 241 243 422 720.2 0 422 244 258 720.2 0 258 245 364 720.2 0 364 246 210 720.2 0 210 247 80 720.2 0 80 248 70 720.2 0 70 249 111 720.2 0 111 250 96 720.2 0 96 251 160 720.2 0 160 252 219 720.2 0 219 253 173 720.2 0 173 254 386 720.2 0 386 255 456 720.2 0 456 256 721 720.2 0 721 257 121 720.2 0 121 258 139 720.2 0 139 259 174 720.2 0 174 260 126 720.2 0 126 261 133 720.2 0 133 262 1308 720.2 0 1308 263 1276 720.2 0 1276 264 1415 720.2 0 1415 265 170 720.2 0 170 266 64 720.2 0 64 267 72 720.2 0 72 268 71 720.2 0 71 269 150 720.2 0 150 270 143 720.2 0 143 271 189 720.2 0 189 272 96 720.2 0 96 273 134 720.2 0 134 274 95 720.2 0 95 275 173 720.2 0 173 276 155 720.2 0 155 277 84 720.2 0 84 278 79 720.2 0 79 279 111 720.2 0 111 280 199 720.2 0 199 281 171 720.2 0 171 282 224 720.2 0 224 283 153 720.2 0 153 284 111 720.2 0 111 285 128 720.2 0 128 286 86 720.2 0 86 287 94 720.2 0 94 288 132 720.2 0 132 289 171 720.2 0 171 290 204 720.2 0 204 291 187 720.2 0 187 292 149 720.2 0 149 293 665 720.2 0 665 294 1158 720.2 0 1158 295 410 720.2 0 410 296 113 720.2 0 113 297 102 720.2 0 102 298 88 720.2 0 88 299 129 720.2 0 129 300 178 720.2 0 178 301 165 720.2 0 165 Finished in 12.01 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 164,189 Reads with adapters: 55,642 (33.9%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 55,606 (33.9%) Total basepairs processed: 45,431,794 bp Total written (filtered): 15,094,197 bp (33.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 55642 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 4 18 32 0.0 3 0 0 20 10 2 19 809 0.0 3 0 736 43 19 11 20 54165 0.0 4 51593 2092 343 107 30 21 132 0.0 4 17 102 7 2 4 22 9 0.0 4 0 1 6 1 1 23 133 0.0 4 0 0 0 98 35 24 1 0.0 4 0 1 27 6 0.0 4 0 0 0 0 6 28 1 0.0 4 0 0 0 1 35 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 87 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 270 0.0 4 0 0 0 0 270 128 1 0.0 4 0 0 0 0 1 129 3 0.0 4 0 0 0 0 3 131 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 16 0.0 4 0 0 0 0 16 160 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 168 1 0.0 4 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 1 198 1 0.0 4 0 0 0 0 1 220 2 0.0 4 0 0 1 0 1 221 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 1 0 1 227 1 0.0 4 0 0 1 228 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 1 273 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.11 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 55,606 Reads with adapters: 11,103 (20.0%) Reads written (passing filters): 55,606 (100.0%) Total basepairs processed: 15,094,197 bp Total written (filtered): 14,903,203 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11103 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.3% C: 6.2% G: 86.4% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 868.8 0 11 4 7 217.2 0 7 5 5 54.3 0 5 7 6 3.4 0 6 9 630 0.2 0 35 595 10 9109 0.1 1 463 8646 11 827 0.0 1 18 809 12 10 0.0 1 1 9 13 22 0.0 1 0 22 18 5 0.0 1 4 1 19 25 0.0 1 16 9 22 32 0.0 1 22 10 23 3 0.0 1 1 2 58 1 0.0 1 1 75 1 0.0 1 1 80 5 0.0 1 4 1 93 4 0.0 1 3 1 114 1 0.0 1 1 123 1 0.0 1 1 150 6 0.0 1 3 3 151 1 0.0 1 0 1 156 11 0.0 1 6 5 189 2 0.0 1 1 1 207 4 0.0 1 3 1 208 337 0.0 1 276 61 209 17 0.0 1 12 5 210 6 0.0 1 4 2 211 1 0.0 1 0 1 223 2 0.0 1 2 226 1 0.0 1 0 1 228 2 0.0 1 2 229 1 0.0 1 1 239 7 0.0 1 7 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.