This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 130.56 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 175,160 (5.9%) Reads that were too short: 13,576 (0.5%) Reads written (passing filters): 161,584 (5.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 45,497,713 bp (5.1%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 175160 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 709 720.2 0 709 7 2061 720.2 0 2061 8 64586 720.2 0 64586 9 79912 720.2 0 79912 10 104 720.2 0 104 11 21 720.2 0 21 12 35 720.2 0 35 13 28 720.2 0 28 14 29 720.2 0 29 15 30 720.2 0 30 16 27 720.2 0 27 17 19 720.2 0 19 18 23 720.2 0 23 19 24 720.2 0 24 20 33 720.2 0 33 21 30 720.2 0 30 22 28 720.2 0 28 23 32 720.2 0 32 24 38 720.2 0 38 25 35 720.2 0 35 26 27 720.2 0 27 27 32 720.2 0 32 28 35 720.2 0 35 29 18 720.2 0 18 30 38 720.2 0 38 31 25 720.2 0 25 32 34 720.2 0 34 33 38 720.2 0 38 34 30 720.2 0 30 35 24 720.2 0 24 36 35 720.2 0 35 37 24 720.2 0 24 38 75 720.2 0 75 39 33 720.2 0 33 40 64 720.2 0 64 41 36 720.2 0 36 42 38 720.2 0 38 43 25 720.2 0 25 44 26 720.2 0 26 45 24 720.2 0 24 46 30 720.2 0 30 47 33 720.2 0 33 48 32 720.2 0 32 49 26 720.2 0 26 50 29 720.2 0 29 51 55 720.2 0 55 52 48 720.2 0 48 53 54 720.2 0 54 54 42 720.2 0 42 55 39 720.2 0 39 56 39 720.2 0 39 57 27 720.2 0 27 58 32 720.2 0 32 59 28 720.2 0 28 60 63 720.2 0 63 61 43 720.2 0 43 62 49 720.2 0 49 63 37 720.2 0 37 64 38 720.2 0 38 65 25 720.2 0 25 66 33 720.2 0 33 67 53 720.2 0 53 68 47 720.2 0 47 69 40 720.2 0 40 70 36 720.2 0 36 71 43 720.2 0 43 72 45 720.2 0 45 73 35 720.2 0 35 74 44 720.2 0 44 75 33 720.2 0 33 76 38 720.2 0 38 77 38 720.2 0 38 78 70 720.2 0 70 79 49 720.2 0 49 80 105 720.2 0 105 81 36 720.2 0 36 82 64 720.2 0 64 83 28 720.2 0 28 84 42 720.2 0 42 85 38 720.2 0 38 86 38 720.2 0 38 87 44 720.2 0 44 88 51 720.2 0 51 89 37 720.2 0 37 90 39 720.2 0 39 91 52 720.2 0 52 92 33 720.2 0 33 93 74 720.2 0 74 94 76 720.2 0 76 95 83 720.2 0 83 96 42 720.2 0 42 97 351 720.2 0 351 98 253 720.2 0 253 99 367 720.2 0 367 100 134 720.2 0 134 101 44 720.2 0 44 102 28 720.2 0 28 103 33 720.2 0 33 104 36 720.2 0 36 105 38 720.2 0 38 106 35 720.2 0 35 107 57 720.2 0 57 108 63 720.2 0 63 109 152 720.2 0 152 110 57 720.2 0 57 111 344 720.2 0 344 112 318 720.2 0 318 113 569 720.2 0 569 114 34 720.2 0 34 115 35 720.2 0 35 116 24 720.2 0 24 117 37 720.2 0 37 118 36 720.2 0 36 119 38 720.2 0 38 120 29 720.2 0 29 121 32 720.2 0 32 122 42 720.2 0 42 123 38 720.2 0 38 124 29 720.2 0 29 125 38 720.2 0 38 126 27 720.2 0 27 127 29 720.2 0 29 128 36 720.2 0 36 129 40 720.2 0 40 130 31 720.2 0 31 131 51 720.2 0 51 132 55 720.2 0 55 133 74 720.2 0 74 134 159 720.2 0 159 135 68 720.2 0 68 136 55 720.2 0 55 137 51 720.2 0 51 138 48 720.2 0 48 139 36 720.2 0 36 140 38 720.2 0 38 141 63 720.2 0 63 142 48 720.2 0 48 143 28 720.2 0 28 144 33 720.2 0 33 145 35 720.2 0 35 146 71 720.2 0 71 147 65 720.2 0 65 148 70 720.2 0 70 149 47 720.2 0 47 150 42 720.2 0 42 151 31 720.2 0 31 152 42 720.2 0 42 153 44 720.2 0 44 154 106 720.2 0 106 155 111 720.2 0 111 156 260 720.2 0 260 157 140 720.2 0 140 158 49 720.2 0 49 159 39 720.2 0 39 160 41 720.2 0 41 161 38 720.2 0 38 162 33 720.2 0 33 163 34 720.2 0 34 164 44 720.2 0 44 165 47 720.2 0 47 166 139 720.2 0 139 167 182 720.2 0 182 168 161 720.2 0 161 169 28 720.2 0 28 170 28 720.2 0 28 171 27 720.2 0 27 172 33 720.2 0 33 173 25 720.2 0 25 174 40 720.2 0 40 175 28 720.2 0 28 176 41 720.2 0 41 177 61 720.2 0 61 178 46 720.2 0 46 179 47 720.2 0 47 180 30 720.2 0 30 181 54 720.2 0 54 182 49 720.2 0 49 183 39 720.2 0 39 184 27 720.2 0 27 185 45 720.2 0 45 186 60 720.2 0 60 187 43 720.2 0 43 188 35 720.2 0 35 189 51 720.2 0 51 190 42 720.2 0 42 191 55 720.2 0 55 192 77 720.2 0 77 193 158 720.2 0 158 194 1573 720.2 0 1573 195 479 720.2 0 479 196 1096 720.2 0 1096 197 62 720.2 0 62 198 121 720.2 0 121 199 62 720.2 0 62 200 82 720.2 0 82 201 68 720.2 0 68 202 63 720.2 0 63 203 35 720.2 0 35 204 50 720.2 0 50 205 39 720.2 0 39 206 40 720.2 0 40 207 44 720.2 0 44 208 38 720.2 0 38 209 37 720.2 0 37 210 40 720.2 0 40 211 39 720.2 0 39 212 27 720.2 0 27 213 38 720.2 0 38 214 33 720.2 0 33 215 54 720.2 0 54 216 88 720.2 0 88 217 79 720.2 0 79 218 54 720.2 0 54 219 45 720.2 0 45 220 145 720.2 0 145 221 266 720.2 0 266 222 200 720.2 0 200 223 207 720.2 0 207 224 40 720.2 0 40 225 40 720.2 0 40 226 42 720.2 0 42 227 28 720.2 0 28 228 53 720.2 0 53 229 41 720.2 0 41 230 46 720.2 0 46 231 34 720.2 0 34 232 39 720.2 0 39 233 36 720.2 0 36 234 32 720.2 0 32 235 42 720.2 0 42 236 64 720.2 0 64 237 234 720.2 0 234 238 321 720.2 0 321 239 341 720.2 0 341 240 86 720.2 0 86 241 94 720.2 0 94 242 126 720.2 0 126 243 213 720.2 0 213 244 110 720.2 0 110 245 165 720.2 0 165 246 122 720.2 0 122 247 50 720.2 0 50 248 45 720.2 0 45 249 76 720.2 0 76 250 72 720.2 0 72 251 120 720.2 0 120 252 154 720.2 0 154 253 141 720.2 0 141 254 151 720.2 0 151 255 153 720.2 0 153 256 212 720.2 0 212 257 82 720.2 0 82 258 82 720.2 0 82 259 108 720.2 0 108 260 78 720.2 0 78 261 86 720.2 0 86 262 1048 720.2 0 1048 263 821 720.2 0 821 264 889 720.2 0 889 265 65 720.2 0 65 266 35 720.2 0 35 267 39 720.2 0 39 268 49 720.2 0 49 269 78 720.2 0 78 270 70 720.2 0 70 271 78 720.2 0 78 272 56 720.2 0 56 273 67 720.2 0 67 274 55 720.2 0 55 275 77 720.2 0 77 276 80 720.2 0 80 277 62 720.2 0 62 278 65 720.2 0 65 279 77 720.2 0 77 280 106 720.2 0 106 281 74 720.2 0 74 282 103 720.2 0 103 283 59 720.2 0 59 284 61 720.2 0 61 285 69 720.2 0 69 286 57 720.2 0 57 287 50 720.2 0 50 288 66 720.2 0 66 289 132 720.2 0 132 290 96 720.2 0 96 291 115 720.2 0 115 292 129 720.2 0 129 293 751 720.2 0 751 294 1467 720.2 0 1467 295 455 720.2 0 455 296 69 720.2 0 69 297 52 720.2 0 52 298 38 720.2 0 38 299 77 720.2 0 77 300 138 720.2 0 138 301 81 720.2 0 81 Finished in 11.21 s (69 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 161,584 Reads with adapters: 64,328 (39.8%) Reads that were too short: 71 (0.0%) Reads written (passing filters): 64,257 (39.8%) Total basepairs processed: 45,497,713 bp Total written (filtered): 17,729,892 bp (39.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 64328 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 8 0.0 3 0 0 6 2 16 333 0.0 3 0 328 5 17 63561 0.0 3 62332 1192 35 2 18 139 0.0 3 7 123 8 1 19 14 0.0 3 2 1 7 4 20 155 0.0 3 0 0 0 155 21 1 0.0 3 0 0 0 1 28 1 0.0 3 1 30 2 0.0 3 1 0 0 1 35 1 0.0 3 0 0 0 1 37 3 0.0 3 1 0 0 2 40 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 55 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 7 0.0 3 0 0 0 7 76 5 0.0 3 0 0 0 5 88 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 96 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 135 1 0.0 3 1 145 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 16 0.0 3 0 0 0 16 165 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 10 0.0 3 0 0 0 10 188 19 0.0 3 0 0 1 18 190 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 199 1 0.0 3 0 1 217 1 0.0 3 0 0 0 1 221 1 0.0 3 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.43 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 64,257 Reads with adapters: 300 (0.5%) Reads written (passing filters): 64,257 (100.0%) Total basepairs processed: 17,729,892 bp Total written (filtered): 17,728,954 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 300 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.3% C: 17.3% G: 35.7% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 265 1004.0 0 265 4 33 251.0 0 33 5 1 62.8 0 1 6 1 15.7 0 1