This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 134.12 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 326,296 (11.1%) Reads that were too short: 18,640 (0.6%) Reads written (passing filters): 307,656 (10.4%) Total basepairs processed: 887,907,860 bp Total written (filtered): 75,465,807 bp (8.5%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 326296 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2019 720.2 0 2019 7 44836 720.2 0 44836 8 27090 720.2 0 27090 9 106 720.2 0 106 10 185 720.2 0 185 11 95 720.2 0 95 12 89 720.2 0 89 13 85 720.2 0 85 14 80 720.2 0 80 15 74 720.2 0 74 16 72 720.2 0 72 17 77 720.2 0 77 18 86 720.2 0 86 19 161 720.2 0 161 20 110 720.2 0 110 21 104 720.2 0 104 22 129 720.2 0 129 23 87 720.2 0 87 24 99 720.2 0 99 25 95 720.2 0 95 26 170 720.2 0 170 27 64 720.2 0 64 28 162 720.2 0 162 29 327 720.2 0 327 30 88 720.2 0 88 31 58 720.2 0 58 32 57 720.2 0 57 33 83 720.2 0 83 34 85 720.2 0 85 35 54 720.2 0 54 36 127 720.2 0 127 37 93 720.2 0 93 38 178 720.2 0 178 39 455 720.2 0 455 40 174 720.2 0 174 41 246 720.2 0 246 42 129 720.2 0 129 43 217 720.2 0 217 44 362 720.2 0 362 45 124 720.2 0 124 46 110 720.2 0 110 47 114 720.2 0 114 48 146 720.2 0 146 49 299 720.2 0 299 50 343 720.2 0 343 51 629 720.2 0 629 52 1084 720.2 0 1084 53 1195 720.2 0 1195 54 2521 720.2 0 2521 55 3756 720.2 0 3756 56 57345 720.2 0 57345 57 28824 720.2 0 28824 58 97862 720.2 0 97862 59 382 720.2 0 382 60 84 720.2 0 84 61 79 720.2 0 79 62 176 720.2 0 176 63 133 720.2 0 133 64 270 720.2 0 270 65 62 720.2 0 62 66 71 720.2 0 71 67 76 720.2 0 76 68 69 720.2 0 69 69 56 720.2 0 56 70 116 720.2 0 116 71 63 720.2 0 63 72 84 720.2 0 84 73 66 720.2 0 66 74 78 720.2 0 78 75 79 720.2 0 79 76 82 720.2 0 82 77 45 720.2 0 45 78 55 720.2 0 55 79 59 720.2 0 59 80 75 720.2 0 75 81 71 720.2 0 71 82 71 720.2 0 71 83 120 720.2 0 120 84 101 720.2 0 101 85 163 720.2 0 163 86 93 720.2 0 93 87 120 720.2 0 120 88 203 720.2 0 203 89 180 720.2 0 180 90 80 720.2 0 80 91 56 720.2 0 56 92 85 720.2 0 85 93 60 720.2 0 60 94 37 720.2 0 37 95 53 720.2 0 53 96 66 720.2 0 66 97 62 720.2 0 62 98 76 720.2 0 76 99 50 720.2 0 50 100 52 720.2 0 52 101 65 720.2 0 65 102 63 720.2 0 63 103 117 720.2 0 117 104 162 720.2 0 162 105 201 720.2 0 201 106 66 720.2 0 66 107 43 720.2 0 43 108 84 720.2 0 84 109 66 720.2 0 66 110 64 720.2 0 64 111 102 720.2 0 102 112 58 720.2 0 58 113 92 720.2 0 92 114 83 720.2 0 83 115 67 720.2 0 67 116 55 720.2 0 55 117 59 720.2 0 59 118 78 720.2 0 78 119 59 720.2 0 59 120 92 720.2 0 92 121 81 720.2 0 81 122 73 720.2 0 73 123 78 720.2 0 78 124 525 720.2 0 525 125 344 720.2 0 344 126 453 720.2 0 453 127 276 720.2 0 276 128 107 720.2 0 107 129 61 720.2 0 61 130 90 720.2 0 90 131 72 720.2 0 72 132 61 720.2 0 61 133 58 720.2 0 58 134 82 720.2 0 82 135 87 720.2 0 87 136 62 720.2 0 62 137 60 720.2 0 60 138 74 720.2 0 74 139 69 720.2 0 69 140 82 720.2 0 82 141 129 720.2 0 129 142 179 720.2 0 179 143 111 720.2 0 111 144 99 720.2 0 99 145 134 720.2 0 134 146 172 720.2 0 172 147 986 720.2 0 986 148 1318 720.2 0 1318 149 2241 720.2 0 2241 150 743 720.2 0 743 151 222 720.2 0 222 152 529 720.2 0 529 153 118 720.2 0 118 154 91 720.2 0 91 155 113 720.2 0 113 156 75 720.2 0 75 157 63 720.2 0 63 158 96 720.2 0 96 159 96 720.2 0 96 160 261 720.2 0 261 161 204 720.2 0 204 162 325 720.2 0 325 163 88 720.2 0 88 164 99 720.2 0 99 165 128 720.2 0 128 166 191 720.2 0 191 167 1942 720.2 0 1942 168 605 720.2 0 605 169 2260 720.2 0 2260 170 132 720.2 0 132 171 149 720.2 0 149 172 224 720.2 0 224 173 286 720.2 0 286 174 165 720.2 0 165 175 304 720.2 0 304 176 302 720.2 0 302 177 469 720.2 0 469 178 133 720.2 0 133 179 75 720.2 0 75 180 122 720.2 0 122 181 77 720.2 0 77 182 88 720.2 0 88 183 86 720.2 0 86 184 86 720.2 0 86 185 87 720.2 0 87 186 161 720.2 0 161 187 99 720.2 0 99 188 134 720.2 0 134 189 134 720.2 0 134 190 104 720.2 0 104 191 145 720.2 0 145 192 175 720.2 0 175 193 207 720.2 0 207 194 225 720.2 0 225 195 564 720.2 0 564 196 969 720.2 0 969 197 760 720.2 0 760 198 371 720.2 0 371 199 368 720.2 0 368 200 2983 720.2 0 2983 201 2164 720.2 0 2164 202 3965 720.2 0 3965 203 355 720.2 0 355 204 61 720.2 0 61 205 63 720.2 0 63 206 88 720.2 0 88 207 94 720.2 0 94 208 68 720.2 0 68 209 55 720.2 0 55 210 53 720.2 0 53 211 57 720.2 0 57 212 57 720.2 0 57 213 64 720.2 0 64 214 75 720.2 0 75 215 64 720.2 0 64 216 79 720.2 0 79 217 81 720.2 0 81 218 67 720.2 0 67 219 164 720.2 0 164 220 168 720.2 0 168 221 220 720.2 0 220 222 103 720.2 0 103 223 44 720.2 0 44 224 85 720.2 0 85 225 85 720.2 0 85 226 71 720.2 0 71 227 69 720.2 0 69 228 101 720.2 0 101 229 275 720.2 0 275 230 262 720.2 0 262 231 445 720.2 0 445 232 510 720.2 0 510 233 232 720.2 0 232 234 478 720.2 0 478 235 88 720.2 0 88 236 41 720.2 0 41 237 46 720.2 0 46 238 65 720.2 0 65 239 88 720.2 0 88 240 88 720.2 0 88 241 138 720.2 0 138 242 97 720.2 0 97 243 121 720.2 0 121 244 75 720.2 0 75 245 60 720.2 0 60 246 63 720.2 0 63 247 114 720.2 0 114 248 114 720.2 0 114 249 94 720.2 0 94 250 100 720.2 0 100 251 84 720.2 0 84 252 88 720.2 0 88 253 41 720.2 0 41 254 52 720.2 0 52 255 59 720.2 0 59 256 66 720.2 0 66 257 76 720.2 0 76 258 96 720.2 0 96 259 82 720.2 0 82 260 62 720.2 0 62 261 101 720.2 0 101 262 97 720.2 0 97 263 85 720.2 0 85 264 83 720.2 0 83 265 97 720.2 0 97 266 102 720.2 0 102 267 79 720.2 0 79 268 198 720.2 0 198 269 243 720.2 0 243 270 221 720.2 0 221 271 178 720.2 0 178 272 99 720.2 0 99 273 134 720.2 0 134 274 127 720.2 0 127 275 172 720.2 0 172 276 234 720.2 0 234 277 291 720.2 0 291 278 350 720.2 0 350 279 167 720.2 0 167 280 256 720.2 0 256 281 425 720.2 0 425 282 647 720.2 0 647 283 459 720.2 0 459 284 103 720.2 0 103 285 82 720.2 0 82 286 57 720.2 0 57 287 85 720.2 0 85 288 340 720.2 0 340 289 346 720.2 0 346 290 210 720.2 0 210 291 164 720.2 0 164 292 131 720.2 0 131 293 204 720.2 0 204 294 291 720.2 0 291 295 176 720.2 0 176 296 105 720.2 0 105 297 93 720.2 0 93 298 61 720.2 0 61 299 80 720.2 0 80 300 156 720.2 0 156 301 355 720.2 0 355 Finished in 19.90 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 307,656 Reads with adapters: 28,295 (9.2%) Reads that were too short: 54 (0.0%) Reads written (passing filters): 28,241 (9.2%) Total basepairs processed: 75,465,807 bp Total written (filtered): 7,699,430 bp (10.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28295 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 22 0.0 3 0 0 13 6 3 19 372 0.0 3 0 312 32 20 8 20 27245 0.0 4 25834 1139 185 73 14 21 79 0.0 4 4 69 5 1 22 13 0.0 4 5 1 5 2 23 247 0.0 4 124 7 0 83 33 24 1 0.0 4 1 25 1 0.0 4 0 0 0 0 1 26 170 0.0 4 0 0 0 0 170 28 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 1 43 1 0.0 4 0 1 45 3 0.0 4 1 0 0 0 2 46 3 0.0 4 0 0 0 1 2 47 1 0.0 4 0 0 0 1 53 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 9 0.0 4 0 0 0 0 9 80 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 122 13 0.0 4 0 0 0 0 13 123 5 0.0 4 0 0 0 0 5 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 127 32 0.0 4 0 0 0 0 32 128 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 1 140 1 0.0 4 1 141 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 7 0.0 4 1 0 0 0 6 155 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 169 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 1 185 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 194 2 0.0 4 0 0 0 0 2 202 15 0.0 4 0 0 0 0 15 207 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 1 254 1 0.0 4 0 0 0 0 1 255 1 0.0 4 1 257 2 0.0 4 1 0 0 0 1 258 1 0.0 4 0 1 263 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.66 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 28,241 Reads with adapters: 7,234 (25.6%) Reads written (passing filters): 28,241 (100.0%) Total basepairs processed: 7,699,430 bp Total written (filtered): 7,318,354 bp (95.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7234 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 44.3% G: 52.0% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 38 441.3 0 38 4 8 110.3 0 8 8 2 0.4 0 2 9 28 0.1 0 1 27 10 143 0.0 1 10 133 11 3617 0.0 1 60 3557 12 27 0.0 1 1 26 13 4 0.0 1 0 4 14 4 0.0 1 0 4 15 1 0.0 1 0 1 19 4 0.0 1 2 2 24 1 0.0 1 1 60 1 0.0 1 1 61 1 0.0 1 1 87 1 0.0 1 0 1 89 6 0.0 1 5 1 90 2829 0.0 1 2408 421 91 103 0.0 1 82 21 92 34 0.0 1 28 6 93 1 0.0 1 1 96 2 0.0 1 2 114 1 0.0 1 1 121 1 0.0 1 1 151 2 0.0 1 0 2 176 1 0.0 1 1 177 248 0.0 1 222 26 178 13 0.0 1 12 1 179 4 0.0 1 4 180 21 0.0 1 19 2 181 1 0.0 1 1 190 1 0.0 1 0 1 207 2 0.0 1 2 208 1 0.0 1 1 209 37 0.0 1 29 8 210 1 0.0 1 1 211 1 0.0 1 1 229 1 0.0 1 1 230 40 0.0 1 37 3 231 1 0.0 1 1 267 2 0.0 1 2