This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 132.93 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 257,743 (8.7%) Reads that were too short: 17,153 (0.6%) Reads written (passing filters): 240,590 (8.2%) Total basepairs processed: 887,907,860 bp Total written (filtered): 63,609,280 bp (7.2%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 257743 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 989 720.2 0 989 7 51394 720.2 0 51394 8 996 720.2 0 996 9 63906 720.2 0 63906 10 98 720.2 0 98 11 42 720.2 0 42 12 67 720.2 0 67 13 54 720.2 0 54 14 44 720.2 0 44 15 55 720.2 0 55 16 68 720.2 0 68 17 63 720.2 0 63 18 66 720.2 0 66 19 44 720.2 0 44 20 59 720.2 0 59 21 72 720.2 0 72 22 50 720.2 0 50 23 63 720.2 0 63 24 63 720.2 0 63 25 69 720.2 0 69 26 63 720.2 0 63 27 82 720.2 0 82 28 121 720.2 0 121 29 191 720.2 0 191 30 118 720.2 0 118 31 166 720.2 0 166 32 1123 720.2 0 1123 33 148 720.2 0 148 34 94 720.2 0 94 35 123 720.2 0 123 36 107 720.2 0 107 37 149 720.2 0 149 38 282 720.2 0 282 39 250 720.2 0 250 40 316 720.2 0 316 41 399 720.2 0 399 42 7130 720.2 0 7130 43 13288 720.2 0 13288 44 6806 720.2 0 6806 45 93 720.2 0 93 46 63 720.2 0 63 47 97 720.2 0 97 48 226 720.2 0 226 49 67 720.2 0 67 50 123 720.2 0 123 51 119 720.2 0 119 52 120 720.2 0 120 53 196 720.2 0 196 54 125 720.2 0 125 55 132 720.2 0 132 56 59 720.2 0 59 57 110 720.2 0 110 58 145 720.2 0 145 59 186 720.2 0 186 60 291 720.2 0 291 61 493 720.2 0 493 62 516 720.2 0 516 63 1060 720.2 0 1060 64 1485 720.2 0 1485 65 28100 720.2 0 28100 66 11512 720.2 0 11512 67 37475 720.2 0 37475 68 191 720.2 0 191 69 58 720.2 0 58 70 43 720.2 0 43 71 79 720.2 0 79 72 46 720.2 0 46 73 51 720.2 0 51 74 41 720.2 0 41 75 42 720.2 0 42 76 75 720.2 0 75 77 100 720.2 0 100 78 56 720.2 0 56 79 31 720.2 0 31 80 46 720.2 0 46 81 55 720.2 0 55 82 41 720.2 0 41 83 55 720.2 0 55 84 48 720.2 0 48 85 50 720.2 0 50 86 45 720.2 0 45 87 517 720.2 0 517 88 70 720.2 0 70 89 89 720.2 0 89 90 62 720.2 0 62 91 47 720.2 0 47 92 42 720.2 0 42 93 62 720.2 0 62 94 63 720.2 0 63 95 64 720.2 0 64 96 34 720.2 0 34 97 48 720.2 0 48 98 84 720.2 0 84 99 61 720.2 0 61 100 64 720.2 0 64 101 90 720.2 0 90 102 83 720.2 0 83 103 41 720.2 0 41 104 52 720.2 0 52 105 33 720.2 0 33 106 46 720.2 0 46 107 54 720.2 0 54 108 81 720.2 0 81 109 39 720.2 0 39 110 63 720.2 0 63 111 70 720.2 0 70 112 80 720.2 0 80 113 43 720.2 0 43 114 51 720.2 0 51 115 38 720.2 0 38 116 38 720.2 0 38 117 42 720.2 0 42 118 74 720.2 0 74 119 57 720.2 0 57 120 50 720.2 0 50 121 52 720.2 0 52 122 35 720.2 0 35 123 47 720.2 0 47 124 69 720.2 0 69 125 42 720.2 0 42 126 55 720.2 0 55 127 50 720.2 0 50 128 86 720.2 0 86 129 77 720.2 0 77 130 70 720.2 0 70 131 73 720.2 0 73 132 49 720.2 0 49 133 72 720.2 0 72 134 213 720.2 0 213 135 267 720.2 0 267 136 241 720.2 0 241 137 110 720.2 0 110 138 150 720.2 0 150 139 111 720.2 0 111 140 247 720.2 0 247 141 47 720.2 0 47 142 32 720.2 0 32 143 55 720.2 0 55 144 45 720.2 0 45 145 38 720.2 0 38 146 43 720.2 0 43 147 46 720.2 0 46 148 85 720.2 0 85 149 40 720.2 0 40 150 37 720.2 0 37 151 36 720.2 0 36 152 33 720.2 0 33 153 44 720.2 0 44 154 51 720.2 0 51 155 33 720.2 0 33 156 34 720.2 0 34 157 49 720.2 0 49 158 66 720.2 0 66 159 61 720.2 0 61 160 48 720.2 0 48 161 36 720.2 0 36 162 28 720.2 0 28 163 38 720.2 0 38 164 33 720.2 0 33 165 53 720.2 0 53 166 24 720.2 0 24 167 30 720.2 0 30 168 59 720.2 0 59 169 66 720.2 0 66 170 75 720.2 0 75 171 27 720.2 0 27 172 45 720.2 0 45 173 36 720.2 0 36 174 46 720.2 0 46 175 40 720.2 0 40 176 26 720.2 0 26 177 36 720.2 0 36 178 65 720.2 0 65 179 85 720.2 0 85 180 78 720.2 0 78 181 55 720.2 0 55 182 36 720.2 0 36 183 52 720.2 0 52 184 52 720.2 0 52 185 59 720.2 0 59 186 79 720.2 0 79 187 35 720.2 0 35 188 32 720.2 0 32 189 46 720.2 0 46 190 34 720.2 0 34 191 35 720.2 0 35 192 34 720.2 0 34 193 30 720.2 0 30 194 30 720.2 0 30 195 29 720.2 0 29 196 49 720.2 0 49 197 68 720.2 0 68 198 77 720.2 0 77 199 67 720.2 0 67 200 61 720.2 0 61 201 69 720.2 0 69 202 120 720.2 0 120 203 147 720.2 0 147 204 75 720.2 0 75 205 56 720.2 0 56 206 43 720.2 0 43 207 48 720.2 0 48 208 34 720.2 0 34 209 60 720.2 0 60 210 49 720.2 0 49 211 47 720.2 0 47 212 48 720.2 0 48 213 60 720.2 0 60 214 36 720.2 0 36 215 62 720.2 0 62 216 57 720.2 0 57 217 77 720.2 0 77 218 70 720.2 0 70 219 92 720.2 0 92 220 110 720.2 0 110 221 72 720.2 0 72 222 327 720.2 0 327 223 402 720.2 0 402 224 510 720.2 0 510 225 408 720.2 0 408 226 52 720.2 0 52 227 48 720.2 0 48 228 59 720.2 0 59 229 48 720.2 0 48 230 44 720.2 0 44 231 46 720.2 0 46 232 34 720.2 0 34 233 41 720.2 0 41 234 42 720.2 0 42 235 54 720.2 0 54 236 61 720.2 0 61 237 62 720.2 0 62 238 58 720.2 0 58 239 64 720.2 0 64 240 83 720.2 0 83 241 82 720.2 0 82 242 260 720.2 0 260 243 331 720.2 0 331 244 254 720.2 0 254 245 165 720.2 0 165 246 47 720.2 0 47 247 85 720.2 0 85 248 50 720.2 0 50 249 48 720.2 0 48 250 83 720.2 0 83 251 117 720.2 0 117 252 170 720.2 0 170 253 200 720.2 0 200 254 489 720.2 0 489 255 1670 720.2 0 1670 256 811 720.2 0 811 257 1583 720.2 0 1583 258 218 720.2 0 218 259 111 720.2 0 111 260 765 720.2 0 765 261 1344 720.2 0 1344 262 739 720.2 0 739 263 119 720.2 0 119 264 83 720.2 0 83 265 121 720.2 0 121 266 154 720.2 0 154 267 88 720.2 0 88 268 51 720.2 0 51 269 63 720.2 0 63 270 314 720.2 0 314 271 427 720.2 0 427 272 351 720.2 0 351 273 68 720.2 0 68 274 73 720.2 0 73 275 39 720.2 0 39 276 69 720.2 0 69 277 138 720.2 0 138 278 151 720.2 0 151 279 118 720.2 0 118 280 55 720.2 0 55 281 41 720.2 0 41 282 43 720.2 0 43 283 47 720.2 0 47 284 52 720.2 0 52 285 33 720.2 0 33 286 37 720.2 0 37 287 49 720.2 0 49 288 42 720.2 0 42 289 38 720.2 0 38 290 57 720.2 0 57 291 83 720.2 0 83 292 93 720.2 0 93 293 81 720.2 0 81 294 89 720.2 0 89 295 39 720.2 0 39 296 51 720.2 0 51 297 62 720.2 0 62 298 51 720.2 0 51 299 77 720.2 0 77 300 96 720.2 0 96 301 82 720.2 0 82 Finished in 16.94 s (70 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 240,590 Reads with adapters: 53,695 (22.3%) Reads that were too short: 134 (0.1%) Reads written (passing filters): 53,561 (22.3%) Total basepairs processed: 63,609,280 bp Total written (filtered): 14,434,488 bp (22.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53695 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 29 0.0 3 0 0 22 6 1 19 642 0.0 3 0 557 54 22 9 20 50473 0.0 4 47840 2191 342 79 21 21 112 0.0 4 18 84 5 4 1 22 12 0.0 4 1 0 8 1 2 23 100 0.0 4 0 0 0 71 29 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 1 45 1 0.0 4 0 0 1 48 1 0.0 4 0 0 0 0 1 57 1 0.0 4 1 68 1 0.0 4 0 0 0 1 79 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 24 0.0 4 0 0 0 0 24 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 1 106 1 0.0 4 0 0 0 0 1 107 37 0.0 4 0 0 0 0 37 108 2 0.0 4 0 0 0 0 2 113 48 0.0 4 0 0 0 0 48 114 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 26 0.0 4 0 0 0 0 26 127 2022 0.0 4 0 0 0 0 2022 128 7 0.0 4 0 0 0 0 7 129 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 167 3 0.0 4 0 0 0 0 3 168 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 59 0.0 4 0 0 0 2 57 194 20 0.0 4 0 0 0 1 19 195 4 0.0 4 1 0 0 0 3 196 1 0.0 4 0 0 0 0 1 200 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 217 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 1 228 6 0.0 4 0 0 0 1 5 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 242 2 0.0 4 0 0 0 0 2 245 1 0.0 4 0 0 0 0 1 261 2 0.0 4 0 0 0 0 2 262 2 0.0 4 0 0 0 0 2 281 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 283 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.90 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 53,561 Reads with adapters: 2,402 (4.5%) Reads written (passing filters): 53,561 (100.0%) Total basepairs processed: 14,434,488 bp Total written (filtered): 14,393,053 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2402 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.9% C: 11.7% G: 74.6% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 242 836.9 0 242 4 23 209.2 0 23 5 2 52.3 0 2 9 11 0.2 0 1 10 10 31 0.1 1 0 31 11 1354 0.0 1 30 1324 12 582 0.0 1 13 569 13 14 0.0 1 0 14 14 2 0.0 1 0 2 15 6 0.0 1 0 6 16 1 0.0 1 0 1 20 9 0.0 1 6 3 21 9 0.0 1 7 2 24 22 0.0 1 19 3 25 4 0.0 1 2 2 40 1 0.0 1 1 75 1 0.0 1 1 79 1 0.0 1 1 109 1 0.0 1 0 1 113 1 0.0 1 1 152 26 0.0 1 17 9 153 1 0.0 1 0 1 171 1 0.0 1 0 1 178 1 0.0 1 1 179 1 0.0 1 0 1 208 1 0.0 1 1 209 1 0.0 1 1 210 36 0.0 1 30 6 225 13 0.0 1 13 261 3 0.0 1 3 273 1 0.0 1 1