This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 133.94 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 280,147 (9.5%) Reads that were too short: 13,010 (0.4%) Reads written (passing filters): 267,137 (9.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 70,039,984 bp (7.9%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 280147 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1022 720.2 0 1022 7 66843 720.2 0 66843 8 659 720.2 0 659 9 53006 720.2 0 53006 10 93 720.2 0 93 11 78 720.2 0 78 12 88 720.2 0 88 13 94 720.2 0 94 14 57 720.2 0 57 15 76 720.2 0 76 16 120 720.2 0 120 17 100 720.2 0 100 18 74 720.2 0 74 19 89 720.2 0 89 20 110 720.2 0 110 21 95 720.2 0 95 22 96 720.2 0 96 23 119 720.2 0 119 24 78 720.2 0 78 25 108 720.2 0 108 26 119 720.2 0 119 27 128 720.2 0 128 28 177 720.2 0 177 29 221 720.2 0 221 30 161 720.2 0 161 31 198 720.2 0 198 32 1233 720.2 0 1233 33 167 720.2 0 167 34 126 720.2 0 126 35 172 720.2 0 172 36 137 720.2 0 137 37 141 720.2 0 141 38 303 720.2 0 303 39 241 720.2 0 241 40 312 720.2 0 312 41 306 720.2 0 306 42 6804 720.2 0 6804 43 12845 720.2 0 12845 44 6558 720.2 0 6558 45 136 720.2 0 136 46 102 720.2 0 102 47 121 720.2 0 121 48 280 720.2 0 280 49 90 720.2 0 90 50 183 720.2 0 183 51 164 720.2 0 164 52 200 720.2 0 200 53 300 720.2 0 300 54 151 720.2 0 151 55 177 720.2 0 177 56 115 720.2 0 115 57 189 720.2 0 189 58 189 720.2 0 189 59 196 720.2 0 196 60 340 720.2 0 340 61 549 720.2 0 549 62 612 720.2 0 612 63 1256 720.2 0 1256 64 1668 720.2 0 1668 65 34928 720.2 0 34928 66 14247 720.2 0 14247 67 47055 720.2 0 47055 68 248 720.2 0 248 69 85 720.2 0 85 70 65 720.2 0 65 71 77 720.2 0 77 72 64 720.2 0 64 73 83 720.2 0 83 74 82 720.2 0 82 75 67 720.2 0 67 76 79 720.2 0 79 77 97 720.2 0 97 78 57 720.2 0 57 79 89 720.2 0 89 80 88 720.2 0 88 81 84 720.2 0 84 82 90 720.2 0 90 83 92 720.2 0 92 84 73 720.2 0 73 85 82 720.2 0 82 86 65 720.2 0 65 87 470 720.2 0 470 88 89 720.2 0 89 89 120 720.2 0 120 90 50 720.2 0 50 91 80 720.2 0 80 92 76 720.2 0 76 93 68 720.2 0 68 94 102 720.2 0 102 95 85 720.2 0 85 96 82 720.2 0 82 97 79 720.2 0 79 98 101 720.2 0 101 99 83 720.2 0 83 100 78 720.2 0 78 101 94 720.2 0 94 102 100 720.2 0 100 103 74 720.2 0 74 104 78 720.2 0 78 105 90 720.2 0 90 106 90 720.2 0 90 107 86 720.2 0 86 108 137 720.2 0 137 109 86 720.2 0 86 110 96 720.2 0 96 111 94 720.2 0 94 112 68 720.2 0 68 113 72 720.2 0 72 114 56 720.2 0 56 115 69 720.2 0 69 116 72 720.2 0 72 117 60 720.2 0 60 118 82 720.2 0 82 119 69 720.2 0 69 120 64 720.2 0 64 121 81 720.2 0 81 122 54 720.2 0 54 123 57 720.2 0 57 124 70 720.2 0 70 125 59 720.2 0 59 126 69 720.2 0 69 127 92 720.2 0 92 128 79 720.2 0 79 129 123 720.2 0 123 130 104 720.2 0 104 131 100 720.2 0 100 132 78 720.2 0 78 133 81 720.2 0 81 134 119 720.2 0 119 135 149 720.2 0 149 136 121 720.2 0 121 137 81 720.2 0 81 138 126 720.2 0 126 139 117 720.2 0 117 140 250 720.2 0 250 141 57 720.2 0 57 142 56 720.2 0 56 143 79 720.2 0 79 144 56 720.2 0 56 145 51 720.2 0 51 146 61 720.2 0 61 147 58 720.2 0 58 148 60 720.2 0 60 149 54 720.2 0 54 150 46 720.2 0 46 151 50 720.2 0 50 152 56 720.2 0 56 153 54 720.2 0 54 154 58 720.2 0 58 155 45 720.2 0 45 156 50 720.2 0 50 157 53 720.2 0 53 158 76 720.2 0 76 159 88 720.2 0 88 160 66 720.2 0 66 161 69 720.2 0 69 162 56 720.2 0 56 163 49 720.2 0 49 164 42 720.2 0 42 165 48 720.2 0 48 166 43 720.2 0 43 167 53 720.2 0 53 168 89 720.2 0 89 169 81 720.2 0 81 170 99 720.2 0 99 171 57 720.2 0 57 172 65 720.2 0 65 173 58 720.2 0 58 174 68 720.2 0 68 175 67 720.2 0 67 176 46 720.2 0 46 177 37 720.2 0 37 178 89 720.2 0 89 179 75 720.2 0 75 180 71 720.2 0 71 181 66 720.2 0 66 182 54 720.2 0 54 183 56 720.2 0 56 184 48 720.2 0 48 185 71 720.2 0 71 186 79 720.2 0 79 187 56 720.2 0 56 188 60 720.2 0 60 189 53 720.2 0 53 190 52 720.2 0 52 191 84 720.2 0 84 192 41 720.2 0 41 193 35 720.2 0 35 194 53 720.2 0 53 195 71 720.2 0 71 196 51 720.2 0 51 197 79 720.2 0 79 198 54 720.2 0 54 199 48 720.2 0 48 200 61 720.2 0 61 201 50 720.2 0 50 202 71 720.2 0 71 203 84 720.2 0 84 204 59 720.2 0 59 205 63 720.2 0 63 206 56 720.2 0 56 207 62 720.2 0 62 208 54 720.2 0 54 209 76 720.2 0 76 210 74 720.2 0 74 211 53 720.2 0 53 212 54 720.2 0 54 213 57 720.2 0 57 214 48 720.2 0 48 215 62 720.2 0 62 216 76 720.2 0 76 217 91 720.2 0 91 218 59 720.2 0 59 219 68 720.2 0 68 220 79 720.2 0 79 221 75 720.2 0 75 222 151 720.2 0 151 223 204 720.2 0 204 224 189 720.2 0 189 225 150 720.2 0 150 226 59 720.2 0 59 227 61 720.2 0 61 228 75 720.2 0 75 229 42 720.2 0 42 230 46 720.2 0 46 231 48 720.2 0 48 232 52 720.2 0 52 233 50 720.2 0 50 234 51 720.2 0 51 235 65 720.2 0 65 236 89 720.2 0 89 237 51 720.2 0 51 238 59 720.2 0 59 239 56 720.2 0 56 240 79 720.2 0 79 241 76 720.2 0 76 242 167 720.2 0 167 243 201 720.2 0 201 244 148 720.2 0 148 245 165 720.2 0 165 246 85 720.2 0 85 247 96 720.2 0 96 248 77 720.2 0 77 249 70 720.2 0 70 250 92 720.2 0 92 251 85 720.2 0 85 252 137 720.2 0 137 253 124 720.2 0 124 254 243 720.2 0 243 255 980 720.2 0 980 256 437 720.2 0 437 257 1010 720.2 0 1010 258 132 720.2 0 132 259 94 720.2 0 94 260 630 720.2 0 630 261 1364 720.2 0 1364 262 658 720.2 0 658 263 111 720.2 0 111 264 81 720.2 0 81 265 122 720.2 0 122 266 122 720.2 0 122 267 72 720.2 0 72 268 53 720.2 0 53 269 42 720.2 0 42 270 196 720.2 0 196 271 185 720.2 0 185 272 159 720.2 0 159 273 61 720.2 0 61 274 58 720.2 0 58 275 52 720.2 0 52 276 69 720.2 0 69 277 75 720.2 0 75 278 80 720.2 0 80 279 90 720.2 0 90 280 72 720.2 0 72 281 67 720.2 0 67 282 56 720.2 0 56 283 56 720.2 0 56 284 52 720.2 0 52 285 46 720.2 0 46 286 48 720.2 0 48 287 44 720.2 0 44 288 54 720.2 0 54 289 46 720.2 0 46 290 54 720.2 0 54 291 86 720.2 0 86 292 93 720.2 0 93 293 72 720.2 0 72 294 86 720.2 0 86 295 41 720.2 0 41 296 52 720.2 0 52 297 55 720.2 0 55 298 73 720.2 0 73 299 88 720.2 0 88 300 89 720.2 0 89 301 83 720.2 0 83 Finished in 17.09 s (64 us/read; 0.94 M reads/minute). === Summary === Total reads processed: 267,137 Reads with adapters: 54,077 (20.2%) Reads that were too short: 77 (0.0%) Reads written (passing filters): 54,000 (20.2%) Total basepairs processed: 70,039,984 bp Total written (filtered): 14,848,593 bp (21.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 54077 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 17 0.0 3 0 0 17 16 393 0.0 3 0 385 8 17 53335 0.0 3 52237 1052 42 4 18 130 0.0 3 2 121 7 19 27 0.0 3 0 0 22 5 20 70 0.0 3 0 0 0 70 26 1 0.0 3 1 27 1 0.0 3 0 1 28 1 0.0 3 0 0 0 1 41 2 0.0 3 2 47 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 1 0.0 3 1 165 1 0.0 3 0 0 0 1 167 4 0.0 3 0 0 0 4 170 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 223 4 0.0 3 0 0 0 4 224 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 261 2 0.0 3 0 0 0 2 262 49 0.0 3 0 0 2 47 263 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.97 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 54,000 Reads with adapters: 1,695 (3.1%) Reads written (passing filters): 54,000 (100.0%) Total basepairs processed: 14,848,593 bp Total written (filtered): 14,843,426 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1695 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.6% C: 5.1% G: 21.4% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1618 843.8 0 1618 4 73 210.9 0 73 5 3 52.7 0 3 6 1 13.2 0 1