This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 135.89 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 174,944 (5.9%) Reads that were too short: 13,186 (0.4%) Reads written (passing filters): 161,758 (5.5%) Total basepairs processed: 887,907,860 bp Total written (filtered): 43,614,424 bp (4.9%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 174944 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2432 720.2 0 2432 7 988 720.2 0 988 8 57962 720.2 0 57962 9 71009 720.2 0 71009 10 96 720.2 0 96 11 34 720.2 0 34 12 37 720.2 0 37 13 34 720.2 0 34 14 19 720.2 0 19 15 30 720.2 0 30 16 27 720.2 0 27 17 32 720.2 0 32 18 31 720.2 0 31 19 43 720.2 0 43 20 35 720.2 0 35 21 39 720.2 0 39 22 34 720.2 0 34 23 30 720.2 0 30 24 38 720.2 0 38 25 35 720.2 0 35 26 42 720.2 0 42 27 35 720.2 0 35 28 41 720.2 0 41 29 50 720.2 0 50 30 37 720.2 0 37 31 26 720.2 0 26 32 13 720.2 0 13 33 35 720.2 0 35 34 40 720.2 0 40 35 36 720.2 0 36 36 47 720.2 0 47 37 44 720.2 0 44 38 30 720.2 0 30 39 36 720.2 0 36 40 35 720.2 0 35 41 21 720.2 0 21 42 41 720.2 0 41 43 40 720.2 0 40 44 26 720.2 0 26 45 43 720.2 0 43 46 42 720.2 0 42 47 28 720.2 0 28 48 26 720.2 0 26 49 36 720.2 0 36 50 29 720.2 0 29 51 39 720.2 0 39 52 33 720.2 0 33 53 50 720.2 0 50 54 38 720.2 0 38 55 41 720.2 0 41 56 42 720.2 0 42 57 56 720.2 0 56 58 42 720.2 0 42 59 24 720.2 0 24 60 29 720.2 0 29 61 35 720.2 0 35 62 41 720.2 0 41 63 41 720.2 0 41 64 48 720.2 0 48 65 53 720.2 0 53 66 44 720.2 0 44 67 65 720.2 0 65 68 44 720.2 0 44 69 133 720.2 0 133 70 94 720.2 0 94 71 105 720.2 0 105 72 53 720.2 0 53 73 44 720.2 0 44 74 37 720.2 0 37 75 60 720.2 0 60 76 62 720.2 0 62 77 87 720.2 0 87 78 110 720.2 0 110 79 88 720.2 0 88 80 553 720.2 0 553 81 466 720.2 0 466 82 1231 720.2 0 1231 83 59 720.2 0 59 84 45 720.2 0 45 85 49 720.2 0 49 86 44 720.2 0 44 87 51 720.2 0 51 88 39 720.2 0 39 89 61 720.2 0 61 90 45 720.2 0 45 91 61 720.2 0 61 92 43 720.2 0 43 93 86 720.2 0 86 94 77 720.2 0 77 95 99 720.2 0 99 96 1145 720.2 0 1145 97 1150 720.2 0 1150 98 1248 720.2 0 1248 99 66 720.2 0 66 100 83 720.2 0 83 101 56 720.2 0 56 102 45 720.2 0 45 103 43 720.2 0 43 104 43 720.2 0 43 105 48 720.2 0 48 106 41 720.2 0 41 107 36 720.2 0 36 108 45 720.2 0 45 109 38 720.2 0 38 110 58 720.2 0 58 111 52 720.2 0 52 112 47 720.2 0 47 113 53 720.2 0 53 114 73 720.2 0 73 115 65 720.2 0 65 116 38 720.2 0 38 117 45 720.2 0 45 118 48 720.2 0 48 119 43 720.2 0 43 120 41 720.2 0 41 121 47 720.2 0 47 122 50 720.2 0 50 123 42 720.2 0 42 124 41 720.2 0 41 125 54 720.2 0 54 126 69 720.2 0 69 127 97 720.2 0 97 128 94 720.2 0 94 129 90 720.2 0 90 130 410 720.2 0 410 131 907 720.2 0 907 132 463 720.2 0 463 133 493 720.2 0 493 134 287 720.2 0 287 135 97 720.2 0 97 136 68 720.2 0 68 137 109 720.2 0 109 138 320 720.2 0 320 139 110 720.2 0 110 140 41 720.2 0 41 141 40 720.2 0 40 142 36 720.2 0 36 143 58 720.2 0 58 144 56 720.2 0 56 145 60 720.2 0 60 146 60 720.2 0 60 147 80 720.2 0 80 148 1053 720.2 0 1053 149 2104 720.2 0 2104 150 745 720.2 0 745 151 35 720.2 0 35 152 58 720.2 0 58 153 48 720.2 0 48 154 55 720.2 0 55 155 41 720.2 0 41 156 32 720.2 0 32 157 37 720.2 0 37 158 50 720.2 0 50 159 64 720.2 0 64 160 39 720.2 0 39 161 56 720.2 0 56 162 39 720.2 0 39 163 43 720.2 0 43 164 56 720.2 0 56 165 34 720.2 0 34 166 49 720.2 0 49 167 46 720.2 0 46 168 31 720.2 0 31 169 35 720.2 0 35 170 52 720.2 0 52 171 42 720.2 0 42 172 40 720.2 0 40 173 68 720.2 0 68 174 64 720.2 0 64 175 55 720.2 0 55 176 47 720.2 0 47 177 67 720.2 0 67 178 52 720.2 0 52 179 46 720.2 0 46 180 62 720.2 0 62 181 49 720.2 0 49 182 52 720.2 0 52 183 83 720.2 0 83 184 85 720.2 0 85 185 112 720.2 0 112 186 143 720.2 0 143 187 971 720.2 0 971 188 4254 720.2 0 4254 189 1784 720.2 0 1784 190 961 720.2 0 961 191 59 720.2 0 59 192 52 720.2 0 52 193 50 720.2 0 50 194 45 720.2 0 45 195 52 720.2 0 52 196 44 720.2 0 44 197 41 720.2 0 41 198 33 720.2 0 33 199 29 720.2 0 29 200 35 720.2 0 35 201 39 720.2 0 39 202 32 720.2 0 32 203 62 720.2 0 62 204 32 720.2 0 32 205 52 720.2 0 52 206 59 720.2 0 59 207 63 720.2 0 63 208 55 720.2 0 55 209 245 720.2 0 245 210 559 720.2 0 559 211 414 720.2 0 414 212 112 720.2 0 112 213 39 720.2 0 39 214 53 720.2 0 53 215 47 720.2 0 47 216 31 720.2 0 31 217 46 720.2 0 46 218 48 720.2 0 48 219 54 720.2 0 54 220 46 720.2 0 46 221 48 720.2 0 48 222 47 720.2 0 47 223 77 720.2 0 77 224 55 720.2 0 55 225 82 720.2 0 82 226 296 720.2 0 296 227 549 720.2 0 549 228 246 720.2 0 246 229 132 720.2 0 132 230 110 720.2 0 110 231 111 720.2 0 111 232 88 720.2 0 88 233 81 720.2 0 81 234 124 720.2 0 124 235 121 720.2 0 121 236 105 720.2 0 105 237 65 720.2 0 65 238 74 720.2 0 74 239 75 720.2 0 75 240 136 720.2 0 136 241 225 720.2 0 225 242 244 720.2 0 244 243 264 720.2 0 264 244 143 720.2 0 143 245 120 720.2 0 120 246 372 720.2 0 372 247 116 720.2 0 116 248 74 720.2 0 74 249 159 720.2 0 159 250 158 720.2 0 158 251 209 720.2 0 209 252 125 720.2 0 125 253 434 720.2 0 434 254 682 720.2 0 682 255 666 720.2 0 666 256 193 720.2 0 193 257 148 720.2 0 148 258 61 720.2 0 61 259 60 720.2 0 60 260 169 720.2 0 169 261 160 720.2 0 160 262 190 720.2 0 190 263 50 720.2 0 50 264 70 720.2 0 70 265 122 720.2 0 122 266 200 720.2 0 200 267 164 720.2 0 164 268 91 720.2 0 91 269 114 720.2 0 114 270 451 720.2 0 451 271 323 720.2 0 323 272 232 720.2 0 232 273 181 720.2 0 181 274 171 720.2 0 171 275 119 720.2 0 119 276 27 720.2 0 27 277 40 720.2 0 40 278 43 720.2 0 43 279 42 720.2 0 42 280 40 720.2 0 40 281 54 720.2 0 54 282 36 720.2 0 36 283 67 720.2 0 67 284 61 720.2 0 61 285 68 720.2 0 68 286 40 720.2 0 40 287 42 720.2 0 42 288 38 720.2 0 38 289 40 720.2 0 40 290 28 720.2 0 28 291 48 720.2 0 48 292 48 720.2 0 48 293 59 720.2 0 59 294 44 720.2 0 44 295 57 720.2 0 57 296 44 720.2 0 44 297 38 720.2 0 38 298 49 720.2 0 49 299 47 720.2 0 47 300 43 720.2 0 43 301 112 720.2 0 112 Finished in 11.42 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 161,758 Reads with adapters: 58,991 (36.5%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 58,965 (36.5%) Total basepairs processed: 43,614,424 bp Total written (filtered): 16,266,487 bp (37.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58991 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 10 1 16 439 0.0 3 0 421 17 1 17 56651 0.0 3 55505 1100 43 3 18 102 0.0 3 5 94 2 1 19 37 0.0 3 12 7 16 2 20 1665 0.0 3 1559 38 3 65 21 9 0.0 3 1 8 23 3 0.0 3 1 0 0 2 34 4 0.0 3 0 0 0 4 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 1 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 70 20 0.0 3 0 0 0 20 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 134 1 0.0 3 1 163 1 0.0 3 0 0 0 1 186 1 0.0 3 1 188 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 195 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.41 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 58,965 Reads with adapters: 708 (1.2%) Reads written (passing filters): 58,965 (100.0%) Total basepairs processed: 16,266,487 bp Total written (filtered): 16,264,213 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 708 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.2% C: 4.5% G: 21.2% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 559 921.3 0 559 4 148 230.3 0 148 5 1 57.6 0 1