This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.51 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 237,430 (8.9%) Reads that were too short: 55,891 (2.1%) Reads written (passing filters): 181,539 (6.8%) Total basepairs processed: 800,111,879 bp Total written (filtered): 45,779,763 bp (5.7%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 237430 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 7780 649.0 0 7780 7 53995 649.0 0 53995 8 963 649.0 0 963 9 63473 649.0 0 63473 10 79 649.0 0 79 11 53 649.0 0 53 12 35 649.0 0 35 13 28 649.0 0 28 14 35 649.0 0 35 15 54 649.0 0 54 16 49 649.0 0 49 17 49 649.0 0 49 18 31 649.0 0 31 19 33 649.0 0 33 20 39 649.0 0 39 21 27 649.0 0 27 22 35 649.0 0 35 23 28 649.0 0 28 24 27 649.0 0 27 25 31 649.0 0 31 26 28 649.0 0 28 27 41 649.0 0 41 28 48 649.0 0 48 29 39 649.0 0 39 30 50 649.0 0 50 31 58 649.0 0 58 32 30 649.0 0 30 33 22 649.0 0 22 34 44 649.0 0 44 35 25 649.0 0 25 36 63 649.0 0 63 37 58 649.0 0 58 38 40 649.0 0 40 39 68 649.0 0 68 40 44 649.0 0 44 41 32 649.0 0 32 42 35 649.0 0 35 43 34 649.0 0 34 44 31 649.0 0 31 45 22 649.0 0 22 46 23 649.0 0 23 47 45 649.0 0 45 48 39 649.0 0 39 49 31 649.0 0 31 50 36 649.0 0 36 51 28 649.0 0 28 52 27 649.0 0 27 53 25 649.0 0 25 54 29 649.0 0 29 55 33 649.0 0 33 56 77 649.0 0 77 57 130 649.0 0 130 58 138 649.0 0 138 59 39 649.0 0 39 60 40 649.0 0 40 61 29 649.0 0 29 62 55 649.0 0 55 63 22 649.0 0 22 64 35 649.0 0 35 65 38 649.0 0 38 66 42 649.0 0 42 67 49 649.0 0 49 68 46 649.0 0 46 69 29 649.0 0 29 70 59 649.0 0 59 71 78 649.0 0 78 72 74 649.0 0 74 73 113 649.0 0 113 74 44 649.0 0 44 75 42 649.0 0 42 76 42 649.0 0 42 77 30 649.0 0 30 78 48 649.0 0 48 79 57 649.0 0 57 80 49 649.0 0 49 81 68 649.0 0 68 82 76 649.0 0 76 83 85 649.0 0 85 84 64 649.0 0 64 85 81 649.0 0 81 86 79 649.0 0 79 87 246 649.0 0 246 88 111 649.0 0 111 89 166 649.0 0 166 90 137 649.0 0 137 91 174 649.0 0 174 92 56 649.0 0 56 93 53 649.0 0 53 94 98 649.0 0 98 95 81 649.0 0 81 96 43 649.0 0 43 97 61 649.0 0 61 98 56 649.0 0 56 99 94 649.0 0 94 100 72 649.0 0 72 101 63 649.0 0 63 102 95 649.0 0 95 103 424 649.0 0 424 104 401 649.0 0 401 105 327 649.0 0 327 106 226 649.0 0 226 107 114 649.0 0 114 108 123 649.0 0 123 109 84 649.0 0 84 110 46 649.0 0 46 111 72 649.0 0 72 112 82 649.0 0 82 113 103 649.0 0 103 114 93 649.0 0 93 115 61 649.0 0 61 116 67 649.0 0 67 117 70 649.0 0 70 118 73 649.0 0 73 119 67 649.0 0 67 120 104 649.0 0 104 121 151 649.0 0 151 122 74 649.0 0 74 123 94 649.0 0 94 124 90 649.0 0 90 125 116 649.0 0 116 126 121 649.0 0 121 127 168 649.0 0 168 128 152 649.0 0 152 129 180 649.0 0 180 130 151 649.0 0 151 131 131 649.0 0 131 132 144 649.0 0 144 133 183 649.0 0 183 134 182 649.0 0 182 135 236 649.0 0 236 136 136 649.0 0 136 137 146 649.0 0 146 138 146 649.0 0 146 139 185 649.0 0 185 140 523 649.0 0 523 141 339 649.0 0 339 142 309 649.0 0 309 143 244 649.0 0 244 144 286 649.0 0 286 145 420 649.0 0 420 146 3597 649.0 0 3597 147 4340 649.0 0 4340 148 4241 649.0 0 4241 149 726 649.0 0 726 150 6645 649.0 0 6645 151 8486 649.0 0 8486 152 5991 649.0 0 5991 153 105 649.0 0 105 154 116 649.0 0 116 155 166 649.0 0 166 156 725 649.0 0 725 157 363 649.0 0 363 158 392 649.0 0 392 159 194 649.0 0 194 160 211 649.0 0 211 161 291 649.0 0 291 162 250 649.0 0 250 163 132 649.0 0 132 164 98 649.0 0 98 165 87 649.0 0 87 166 130 649.0 0 130 167 92 649.0 0 92 168 102 649.0 0 102 169 65 649.0 0 65 170 79 649.0 0 79 171 76 649.0 0 76 172 70 649.0 0 70 173 80 649.0 0 80 174 97 649.0 0 97 175 900 649.0 0 900 176 257 649.0 0 257 177 581 649.0 0 581 178 92 649.0 0 92 179 100 649.0 0 100 180 117 649.0 0 117 181 208 649.0 0 208 182 175 649.0 0 175 183 203 649.0 0 203 184 177 649.0 0 177 185 160 649.0 0 160 186 309 649.0 0 309 187 171 649.0 0 171 188 114 649.0 0 114 189 86 649.0 0 86 190 82 649.0 0 82 191 77 649.0 0 77 192 100 649.0 0 100 193 68 649.0 0 68 194 98 649.0 0 98 195 77 649.0 0 77 196 72 649.0 0 72 197 71 649.0 0 71 198 79 649.0 0 79 199 97 649.0 0 97 200 120 649.0 0 120 201 121 649.0 0 121 202 234 649.0 0 234 203 253 649.0 0 253 204 197 649.0 0 197 205 142 649.0 0 142 206 234 649.0 0 234 207 198 649.0 0 198 208 318 649.0 0 318 209 185 649.0 0 185 210 143 649.0 0 143 211 156 649.0 0 156 212 428 649.0 0 428 213 287 649.0 0 287 214 220 649.0 0 220 215 111 649.0 0 111 216 91 649.0 0 91 217 95 649.0 0 95 218 88 649.0 0 88 219 170 649.0 0 170 220 304 649.0 0 304 221 279 649.0 0 279 222 275 649.0 0 275 223 148 649.0 0 148 224 168 649.0 0 168 225 196 649.0 0 196 226 229 649.0 0 229 227 140 649.0 0 140 228 118 649.0 0 118 229 89 649.0 0 89 230 128 649.0 0 128 231 120 649.0 0 120 232 140 649.0 0 140 233 185 649.0 0 185 234 237 649.0 0 237 235 206 649.0 0 206 236 191 649.0 0 191 237 147 649.0 0 147 238 169 649.0 0 169 239 143 649.0 0 143 240 120 649.0 0 120 241 186 649.0 0 186 242 122 649.0 0 122 243 218 649.0 0 218 244 178 649.0 0 178 245 153 649.0 0 153 246 116 649.0 0 116 247 136 649.0 0 136 248 139 649.0 0 139 249 172 649.0 0 172 250 175 649.0 0 175 251 225 649.0 0 225 252 298 649.0 0 298 253 416 649.0 0 416 254 765 649.0 0 765 255 2975 649.0 0 2975 256 10100 649.0 0 10100 257 11680 649.0 0 11680 258 8489 649.0 0 8489 259 2575 649.0 0 2575 260 269 649.0 0 269 261 226 649.0 0 226 262 397 649.0 0 397 263 455 649.0 0 455 264 275 649.0 0 275 265 148 649.0 0 148 266 204 649.0 0 204 267 263 649.0 0 263 268 291 649.0 0 291 269 233 649.0 0 233 270 117 649.0 0 117 271 100 649.0 0 100 272 107 649.0 0 107 273 166 649.0 0 166 274 199 649.0 0 199 275 208 649.0 0 208 276 146 649.0 0 146 277 126 649.0 0 126 278 140 649.0 0 140 279 266 649.0 0 266 280 628 649.0 0 628 281 625 649.0 0 625 282 414 649.0 0 414 283 330 649.0 0 330 284 317 649.0 0 317 285 286 649.0 0 286 286 133 649.0 0 133 287 144 649.0 0 144 288 202 649.0 0 202 289 165 649.0 0 165 290 150 649.0 0 150 291 88 649.0 0 88 292 135 649.0 0 135 293 138 649.0 0 138 294 238 649.0 0 238 295 150 649.0 0 150 296 183 649.0 0 183 297 175 649.0 0 175 298 166 649.0 0 166 299 123 649.0 0 123 300 117 649.0 0 117 301 248 649.0 0 248 Finished in 8.69 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 181,539 Reads with adapters: 61,221 (33.7%) Reads that were too short: 74 (0.0%) Reads written (passing filters): 61,147 (33.7%) Total basepairs processed: 45,779,763 bp Total written (filtered): 16,704,534 bp (36.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 61221 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 5 0.0 3 0 0 0 3 2 18 42 0.0 3 0 0 33 4 5 19 633 0.0 3 0 556 41 23 13 20 59425 0.0 4 56017 2725 506 137 40 21 154 0.0 4 20 124 5 2 3 22 17 0.0 4 0 1 16 23 362 0.0 4 236 7 0 85 34 24 3 0.0 4 0 0 0 1 2 25 2 0.0 4 0 0 1 1 28 20 0.0 4 0 0 0 0 20 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 11 0.0 4 0 0 0 0 11 61 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 9 0.0 4 0 0 0 0 9 127 455 0.0 4 0 0 0 2 453 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 1 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 1 152 1 0.0 4 0 0 0 1 153 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 221 2 0.0 4 0 0 0 0 2 222 4 0.0 4 0 0 0 1 3 223 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 15 0.0 4 0 0 0 1 14 229 5 0.0 4 0 0 0 2 3 230 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.10 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 61,147 Reads with adapters: 2,139 (3.5%) Reads written (passing filters): 61,147 (100.0%) Total basepairs processed: 16,704,534 bp Total written (filtered): 16,614,220 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2139 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 19.0% C: 15.5% G: 58.3% T: 6.5% none/other: 0.8% Overview of removed sequences length count expect max.err error counts 3 290 955.4 0 290 4 39 238.9 0 39 5 11 59.7 0 11 6 1 14.9 0 1 7 1 3.7 0 1 9 13 0.2 0 2 11 10 87 0.1 1 3 84 11 625 0.0 1 20 605 12 614 0.0 1 21 593 13 69 0.0 1 2 67 14 2 0.0 1 0 2 15 10 0.0 1 0 10 16 2 0.0 1 0 2 20 29 0.0 1 22 7 21 9 0.0 1 5 4 24 22 0.0 1 19 3 95 2 0.0 1 2 108 1 0.0 1 0 1 127 1 0.0 1 0 1 128 1 0.0 1 0 1 144 1 0.0 1 1 152 19 0.0 1 15 4 153 2 0.0 1 2 158 1 0.0 1 0 1 186 9 0.0 1 7 2 187 2 0.0 1 1 1 210 132 0.0 1 93 39 211 22 0.0 1 16 6 212 1 0.0 1 1 225 2 0.0 1 1 1 268 5 0.0 1 5 269 3 0.0 1 1 2 271 6 0.0 1 6 272 2 0.0 1 2 273 77 0.0 1 72 5 274 23 0.0 1 19 4 275 3 0.0 1 3