This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.81 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 151,846 (5.7%) Reads that were too short: 16,227 (0.6%) Reads written (passing filters): 135,619 (5.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 29,702,342 bp (3.7%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 151846 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2457 649.0 0 2457 7 30916 649.0 0 30916 8 1526 649.0 0 1526 9 27934 649.0 0 27934 10 72 649.0 0 72 11 25 649.0 0 25 12 27 649.0 0 27 13 31 649.0 0 31 14 18 649.0 0 18 15 34 649.0 0 34 16 17 649.0 0 17 17 18 649.0 0 18 18 30 649.0 0 30 19 22 649.0 0 22 20 26 649.0 0 26 21 15 649.0 0 15 22 18 649.0 0 18 23 38 649.0 0 38 24 21 649.0 0 21 25 21 649.0 0 21 26 21 649.0 0 21 27 33 649.0 0 33 28 15 649.0 0 15 29 43 649.0 0 43 30 45 649.0 0 45 31 24 649.0 0 24 32 27 649.0 0 27 33 32 649.0 0 32 34 32 649.0 0 32 35 26 649.0 0 26 36 23 649.0 0 23 37 37 649.0 0 37 38 36 649.0 0 36 39 35 649.0 0 35 40 130 649.0 0 130 41 28 649.0 0 28 42 47 649.0 0 47 43 16 649.0 0 16 44 25 649.0 0 25 45 27 649.0 0 27 46 36 649.0 0 36 47 51 649.0 0 51 48 34 649.0 0 34 49 39 649.0 0 39 50 57 649.0 0 57 51 33 649.0 0 33 52 28 649.0 0 28 53 24 649.0 0 24 54 24 649.0 0 24 55 97 649.0 0 97 56 73 649.0 0 73 57 79 649.0 0 79 58 38 649.0 0 38 59 40 649.0 0 40 60 55 649.0 0 55 61 29 649.0 0 29 62 24 649.0 0 24 63 28 649.0 0 28 64 27 649.0 0 27 65 52 649.0 0 52 66 98 649.0 0 98 67 36 649.0 0 36 68 60 649.0 0 60 69 51 649.0 0 51 70 40 649.0 0 40 71 60 649.0 0 60 72 33 649.0 0 33 73 46 649.0 0 46 74 80 649.0 0 80 75 72 649.0 0 72 76 33 649.0 0 33 77 22 649.0 0 22 78 20 649.0 0 20 79 31 649.0 0 31 80 20 649.0 0 20 81 35 649.0 0 35 82 56 649.0 0 56 83 73 649.0 0 73 84 54 649.0 0 54 85 38 649.0 0 38 86 32 649.0 0 32 87 29 649.0 0 29 88 36 649.0 0 36 89 45 649.0 0 45 90 66 649.0 0 66 91 130 649.0 0 130 92 52 649.0 0 52 93 54 649.0 0 54 94 31 649.0 0 31 95 53 649.0 0 53 96 26 649.0 0 26 97 43 649.0 0 43 98 26 649.0 0 26 99 26 649.0 0 26 100 30 649.0 0 30 101 36 649.0 0 36 102 63 649.0 0 63 103 286 649.0 0 286 104 77 649.0 0 77 105 626 649.0 0 626 106 328 649.0 0 328 107 360 649.0 0 360 108 30 649.0 0 30 109 27 649.0 0 27 110 36 649.0 0 36 111 56 649.0 0 56 112 226 649.0 0 226 113 93 649.0 0 93 114 257 649.0 0 257 115 68 649.0 0 68 116 86 649.0 0 86 117 41 649.0 0 41 118 42 649.0 0 42 119 40 649.0 0 40 120 36 649.0 0 36 121 50 649.0 0 50 122 38 649.0 0 38 123 47 649.0 0 47 124 39 649.0 0 39 125 49 649.0 0 49 126 66 649.0 0 66 127 97 649.0 0 97 128 120 649.0 0 120 129 210 649.0 0 210 130 118 649.0 0 118 131 102 649.0 0 102 132 129 649.0 0 129 133 166 649.0 0 166 134 376 649.0 0 376 135 117 649.0 0 117 136 158 649.0 0 158 137 272 649.0 0 272 138 113 649.0 0 113 139 134 649.0 0 134 140 212 649.0 0 212 141 199 649.0 0 199 142 309 649.0 0 309 143 329 649.0 0 329 144 476 649.0 0 476 145 592 649.0 0 592 146 1008 649.0 0 1008 147 20261 649.0 0 20261 148 9237 649.0 0 9237 149 21296 649.0 0 21296 150 408 649.0 0 408 151 98 649.0 0 98 152 409 649.0 0 409 153 110 649.0 0 110 154 416 649.0 0 416 155 214 649.0 0 214 156 166 649.0 0 166 157 49 649.0 0 49 158 32 649.0 0 32 159 56 649.0 0 56 160 47 649.0 0 47 161 38 649.0 0 38 162 38 649.0 0 38 163 48 649.0 0 48 164 44 649.0 0 44 165 49 649.0 0 49 166 36 649.0 0 36 167 51 649.0 0 51 168 148 649.0 0 148 169 130 649.0 0 130 170 160 649.0 0 160 171 174 649.0 0 174 172 271 649.0 0 271 173 132 649.0 0 132 174 158 649.0 0 158 175 81 649.0 0 81 176 27 649.0 0 27 177 115 649.0 0 115 178 64 649.0 0 64 179 71 649.0 0 71 180 38 649.0 0 38 181 33 649.0 0 33 182 67 649.0 0 67 183 70 649.0 0 70 184 74 649.0 0 74 185 59 649.0 0 59 186 133 649.0 0 133 187 128 649.0 0 128 188 1003 649.0 0 1003 189 695 649.0 0 695 190 643 649.0 0 643 191 152 649.0 0 152 192 56 649.0 0 56 193 35 649.0 0 35 194 37 649.0 0 37 195 49 649.0 0 49 196 112 649.0 0 112 197 113 649.0 0 113 198 115 649.0 0 115 199 261 649.0 0 261 200 400 649.0 0 400 201 1899 649.0 0 1899 202 1770 649.0 0 1770 203 1365 649.0 0 1365 204 486 649.0 0 486 205 104 649.0 0 104 206 64 649.0 0 64 207 42 649.0 0 42 208 114 649.0 0 114 209 44 649.0 0 44 210 56 649.0 0 56 211 29 649.0 0 29 212 22 649.0 0 22 213 21 649.0 0 21 214 40 649.0 0 40 215 50 649.0 0 50 216 43 649.0 0 43 217 37 649.0 0 37 218 24 649.0 0 24 219 46 649.0 0 46 220 41 649.0 0 41 221 68 649.0 0 68 222 60 649.0 0 60 223 81 649.0 0 81 224 84 649.0 0 84 225 98 649.0 0 98 226 157 649.0 0 157 227 178 649.0 0 178 228 171 649.0 0 171 229 236 649.0 0 236 230 115 649.0 0 115 231 71 649.0 0 71 232 47 649.0 0 47 233 45 649.0 0 45 234 42 649.0 0 42 235 53 649.0 0 53 236 73 649.0 0 73 237 48 649.0 0 48 238 62 649.0 0 62 239 44 649.0 0 44 240 34 649.0 0 34 241 29 649.0 0 29 242 35 649.0 0 35 243 78 649.0 0 78 244 74 649.0 0 74 245 70 649.0 0 70 246 44 649.0 0 44 247 88 649.0 0 88 248 95 649.0 0 95 249 105 649.0 0 105 250 120 649.0 0 120 251 113 649.0 0 113 252 98 649.0 0 98 253 71 649.0 0 71 254 90 649.0 0 90 255 24 649.0 0 24 256 57 649.0 0 57 257 80 649.0 0 80 258 180 649.0 0 180 259 150 649.0 0 150 260 142 649.0 0 142 261 90 649.0 0 90 262 84 649.0 0 84 263 60 649.0 0 60 264 78 649.0 0 78 265 62 649.0 0 62 266 69 649.0 0 69 267 60 649.0 0 60 268 74 649.0 0 74 269 556 649.0 0 556 270 275 649.0 0 275 271 227 649.0 0 227 272 89 649.0 0 89 273 94 649.0 0 94 274 112 649.0 0 112 275 199 649.0 0 199 276 160 649.0 0 160 277 125 649.0 0 125 278 77 649.0 0 77 279 94 649.0 0 94 280 135 649.0 0 135 281 96 649.0 0 96 282 350 649.0 0 350 283 208 649.0 0 208 284 168 649.0 0 168 285 71 649.0 0 71 286 147 649.0 0 147 287 94 649.0 0 94 288 167 649.0 0 167 289 167 649.0 0 167 290 102 649.0 0 102 291 474 649.0 0 474 292 487 649.0 0 487 293 565 649.0 0 565 294 847 649.0 0 847 295 621 649.0 0 621 296 486 649.0 0 486 297 185 649.0 0 185 298 59 649.0 0 59 299 94 649.0 0 94 300 111 649.0 0 111 301 100 649.0 0 100 Finished in 6.18 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 135,619 Reads with adapters: 30,466 (22.5%) Reads that were too short: 74 (0.1%) Reads written (passing filters): 30,392 (22.4%) Total basepairs processed: 29,702,342 bp Total written (filtered): 8,217,781 bp (27.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30466 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 33 0.0 3 0 0 26 4 3 19 376 0.0 3 0 340 15 14 7 20 29328 0.0 4 27921 1159 195 41 12 21 62 0.0 4 11 49 2 22 19 0.0 4 8 2 7 1 1 23 77 0.0 4 0 0 0 67 10 24 1 0.0 4 1 26 1 0.0 4 0 0 0 1 32 3 0.0 4 0 0 0 0 3 42 1 0.0 4 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 69 6 0.0 4 0 0 0 0 6 70 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 89 16 0.0 4 0 0 0 0 16 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 472 0.0 4 0 0 0 0 472 128 2 0.0 4 0 0 0 0 2 130 3 0.0 4 0 0 0 0 3 137 2 0.0 4 0 0 0 0 2 138 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 1 151 1 0.0 4 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 1 157 1 0.0 4 0 0 1 196 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 1 216 1 0.0 4 0 0 0 0 1 219 1 0.0 4 1 227 2 0.0 4 1 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 1 250 2 0.0 4 0 0 0 0 2 251 4 0.0 4 0 0 2 0 2 252 1 0.0 4 0 0 0 1 253 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 1 1 279 1 0.0 4 0 1 282 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.13 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 30,392 Reads with adapters: 7,018 (23.1%) Reads written (passing filters): 30,392 (100.0%) Total basepairs processed: 8,217,781 bp Total written (filtered): 8,105,141 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7018 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 8.8% G: 86.9% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 32 474.9 0 32 4 4 118.7 0 4 6 1 7.4 0 1 8 4 0.5 0 4 9 733 0.1 0 70 663 10 4605 0.0 1 334 4271 11 1398 0.0 1 47 1351 12 17 0.0 1 1 16 13 17 0.0 1 0 17 14 1 0.0 1 0 1 15 1 0.0 1 0 1 18 7 0.0 1 4 3 19 2 0.0 1 1 1 22 2 0.0 1 1 1 86 1 0.0 1 1 150 10 0.0 1 9 1 152 1 0.0 1 1 207 14 0.0 1 11 3 208 10 0.0 1 9 1 209 1 0.0 1 0 1 232 8 0.0 1 7 1 234 3 0.0 1 3 235 137 0.0 1 125 12 236 8 0.0 1 8 237 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.