This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.89 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 142,235 (5.4%) Reads that were too short: 8,909 (0.3%) Reads written (passing filters): 133,326 (5.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 29,562,383 bp (3.7%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 142235 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2228 649.0 0 2228 7 29224 649.0 0 29224 8 1256 649.0 0 1256 9 32554 649.0 0 32554 10 54 649.0 0 54 11 19 649.0 0 19 12 15 649.0 0 15 13 9 649.0 0 9 14 10 649.0 0 10 15 24 649.0 0 24 16 12 649.0 0 12 17 13 649.0 0 13 18 15 649.0 0 15 19 13 649.0 0 13 20 9 649.0 0 9 21 17 649.0 0 17 22 14 649.0 0 14 23 10 649.0 0 10 24 13 649.0 0 13 25 9 649.0 0 9 26 15 649.0 0 15 27 16 649.0 0 16 28 16 649.0 0 16 29 12 649.0 0 12 30 19 649.0 0 19 31 15 649.0 0 15 32 18 649.0 0 18 33 13 649.0 0 13 34 20 649.0 0 20 35 17 649.0 0 17 36 16 649.0 0 16 37 23 649.0 0 23 38 20 649.0 0 20 39 13 649.0 0 13 40 35 649.0 0 35 41 20 649.0 0 20 42 18 649.0 0 18 43 15 649.0 0 15 44 10 649.0 0 10 45 8 649.0 0 8 46 22 649.0 0 22 47 35 649.0 0 35 48 24 649.0 0 24 49 20 649.0 0 20 50 51 649.0 0 51 51 24 649.0 0 24 52 20 649.0 0 20 53 13 649.0 0 13 54 13 649.0 0 13 55 40 649.0 0 40 56 49 649.0 0 49 57 31 649.0 0 31 58 23 649.0 0 23 59 23 649.0 0 23 60 16 649.0 0 16 61 13 649.0 0 13 62 12 649.0 0 12 63 14 649.0 0 14 64 12 649.0 0 12 65 33 649.0 0 33 66 60 649.0 0 60 67 14 649.0 0 14 68 15 649.0 0 15 69 25 649.0 0 25 70 15 649.0 0 15 71 43 649.0 0 43 72 24 649.0 0 24 73 18 649.0 0 18 74 56 649.0 0 56 75 42 649.0 0 42 76 21 649.0 0 21 77 20 649.0 0 20 78 18 649.0 0 18 79 28 649.0 0 28 80 23 649.0 0 23 81 37 649.0 0 37 82 46 649.0 0 46 83 24 649.0 0 24 84 19 649.0 0 19 85 26 649.0 0 26 86 16 649.0 0 16 87 31 649.0 0 31 88 23 649.0 0 23 89 29 649.0 0 29 90 34 649.0 0 34 91 48 649.0 0 48 92 18 649.0 0 18 93 32 649.0 0 32 94 27 649.0 0 27 95 23 649.0 0 23 96 25 649.0 0 25 97 21 649.0 0 21 98 18 649.0 0 18 99 12 649.0 0 12 100 15 649.0 0 15 101 32 649.0 0 32 102 38 649.0 0 38 103 265 649.0 0 265 104 38 649.0 0 38 105 249 649.0 0 249 106 164 649.0 0 164 107 179 649.0 0 179 108 23 649.0 0 23 109 27 649.0 0 27 110 35 649.0 0 35 111 29 649.0 0 29 112 68 649.0 0 68 113 59 649.0 0 59 114 130 649.0 0 130 115 41 649.0 0 41 116 58 649.0 0 58 117 43 649.0 0 43 118 23 649.0 0 23 119 27 649.0 0 27 120 33 649.0 0 33 121 36 649.0 0 36 122 29 649.0 0 29 123 64 649.0 0 64 124 35 649.0 0 35 125 29 649.0 0 29 126 36 649.0 0 36 127 69 649.0 0 69 128 102 649.0 0 102 129 154 649.0 0 154 130 106 649.0 0 106 131 81 649.0 0 81 132 88 649.0 0 88 133 180 649.0 0 180 134 374 649.0 0 374 135 118 649.0 0 118 136 112 649.0 0 112 137 253 649.0 0 253 138 96 649.0 0 96 139 108 649.0 0 108 140 252 649.0 0 252 141 202 649.0 0 202 142 257 649.0 0 257 143 305 649.0 0 305 144 414 649.0 0 414 145 470 649.0 0 470 146 768 649.0 0 768 147 20534 649.0 0 20534 148 9385 649.0 0 9385 149 22317 649.0 0 22317 150 371 649.0 0 371 151 94 649.0 0 94 152 342 649.0 0 342 153 48 649.0 0 48 154 297 649.0 0 297 155 164 649.0 0 164 156 124 649.0 0 124 157 19 649.0 0 19 158 23 649.0 0 23 159 28 649.0 0 28 160 21 649.0 0 21 161 28 649.0 0 28 162 28 649.0 0 28 163 32 649.0 0 32 164 19 649.0 0 19 165 26 649.0 0 26 166 32 649.0 0 32 167 36 649.0 0 36 168 99 649.0 0 99 169 75 649.0 0 75 170 123 649.0 0 123 171 192 649.0 0 192 172 167 649.0 0 167 173 61 649.0 0 61 174 113 649.0 0 113 175 43 649.0 0 43 176 14 649.0 0 14 177 35 649.0 0 35 178 40 649.0 0 40 179 35 649.0 0 35 180 21 649.0 0 21 181 18 649.0 0 18 182 34 649.0 0 34 183 39 649.0 0 39 184 46 649.0 0 46 185 35 649.0 0 35 186 64 649.0 0 64 187 67 649.0 0 67 188 762 649.0 0 762 189 481 649.0 0 481 190 523 649.0 0 523 191 110 649.0 0 110 192 22 649.0 0 22 193 23 649.0 0 23 194 18 649.0 0 18 195 26 649.0 0 26 196 39 649.0 0 39 197 77 649.0 0 77 198 71 649.0 0 71 199 135 649.0 0 135 200 264 649.0 0 264 201 1896 649.0 0 1896 202 1843 649.0 0 1843 203 1400 649.0 0 1400 204 398 649.0 0 398 205 42 649.0 0 42 206 20 649.0 0 20 207 12 649.0 0 12 208 34 649.0 0 34 209 16 649.0 0 16 210 33 649.0 0 33 211 15 649.0 0 15 212 27 649.0 0 27 213 18 649.0 0 18 214 20 649.0 0 20 215 27 649.0 0 27 216 21 649.0 0 21 217 18 649.0 0 18 218 13 649.0 0 13 219 23 649.0 0 23 220 16 649.0 0 16 221 33 649.0 0 33 222 45 649.0 0 45 223 19 649.0 0 19 224 29 649.0 0 29 225 40 649.0 0 40 226 88 649.0 0 88 227 163 649.0 0 163 228 137 649.0 0 137 229 129 649.0 0 129 230 69 649.0 0 69 231 24 649.0 0 24 232 31 649.0 0 31 233 18 649.0 0 18 234 30 649.0 0 30 235 27 649.0 0 27 236 49 649.0 0 49 237 31 649.0 0 31 238 55 649.0 0 55 239 36 649.0 0 36 240 22 649.0 0 22 241 31 649.0 0 31 242 24 649.0 0 24 243 26 649.0 0 26 244 27 649.0 0 27 245 36 649.0 0 36 246 19 649.0 0 19 247 32 649.0 0 32 248 33 649.0 0 33 249 40 649.0 0 40 250 45 649.0 0 45 251 45 649.0 0 45 252 44 649.0 0 44 253 17 649.0 0 17 254 40 649.0 0 40 255 22 649.0 0 22 256 34 649.0 0 34 257 54 649.0 0 54 258 103 649.0 0 103 259 116 649.0 0 116 260 50 649.0 0 50 261 35 649.0 0 35 262 25 649.0 0 25 263 21 649.0 0 21 264 26 649.0 0 26 265 21 649.0 0 21 266 20 649.0 0 20 267 35 649.0 0 35 268 30 649.0 0 30 269 226 649.0 0 226 270 104 649.0 0 104 271 78 649.0 0 78 272 30 649.0 0 30 273 45 649.0 0 45 274 64 649.0 0 64 275 76 649.0 0 76 276 60 649.0 0 60 277 69 649.0 0 69 278 40 649.0 0 40 279 31 649.0 0 31 280 46 649.0 0 46 281 31 649.0 0 31 282 152 649.0 0 152 283 84 649.0 0 84 284 67 649.0 0 67 285 45 649.0 0 45 286 64 649.0 0 64 287 40 649.0 0 40 288 86 649.0 0 86 289 67 649.0 0 67 290 33 649.0 0 33 291 180 649.0 0 180 292 150 649.0 0 150 293 218 649.0 0 218 294 270 649.0 0 270 295 131 649.0 0 131 296 100 649.0 0 100 297 66 649.0 0 66 298 18 649.0 0 18 299 39 649.0 0 39 300 39 649.0 0 39 301 38 649.0 0 38 Finished in 5.55 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 133,326 Reads with adapters: 31,070 (23.3%) Reads that were too short: 58 (0.0%) Reads written (passing filters): 31,012 (23.3%) Total basepairs processed: 29,562,383 bp Total written (filtered): 8,558,712 bp (29.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31070 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 11 0.0 3 0 0 10 1 16 179 0.0 3 0 176 3 17 30470 0.0 3 29846 605 16 3 18 77 0.0 3 4 67 4 2 19 34 0.0 3 5 0 9 20 20 57 0.0 3 0 1 0 56 22 2 0.0 3 0 0 1 1 28 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 8 0.0 3 0 0 0 8 44 1 0.0 3 0 0 0 1 45 120 0.0 3 0 0 1 119 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 79 31 0.0 3 1 0 0 30 80 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 6 0.0 3 0 0 0 6 92 1 0.0 3 1 100 1 0.0 3 0 0 0 1 107 1 0.0 3 1 118 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 144 1 0.0 3 1 150 1 0.0 3 0 0 0 1 156 12 0.0 3 0 0 0 12 159 6 0.0 3 0 0 0 6 182 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 5 0.0 3 0 0 0 5 271 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 31,012 Reads with adapters: 191 (0.6%) Reads written (passing filters): 31,012 (100.0%) Total basepairs processed: 8,558,712 bp Total written (filtered): 8,558,124 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 191 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.1% C: 18.3% G: 28.8% T: 28.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 181 484.6 0 181 4 5 121.1 0 5 5 5 30.3 0 5