This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.20 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 289,325 (10.9%) Reads that were too short: 64,797 (2.4%) Reads written (passing filters): 224,528 (8.4%) Total basepairs processed: 800,111,879 bp Total written (filtered): 58,228,365 bp (7.3%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 289325 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1856 649.0 0 1856 7 78230 649.0 0 78230 8 1888 649.0 0 1888 9 83003 649.0 0 83003 10 101 649.0 0 101 11 23 649.0 0 23 12 18 649.0 0 18 13 18 649.0 0 18 14 21 649.0 0 21 15 20 649.0 0 20 16 18 649.0 0 18 17 20 649.0 0 20 18 17 649.0 0 17 19 10 649.0 0 10 20 17 649.0 0 17 21 11 649.0 0 11 22 19 649.0 0 19 23 19 649.0 0 19 24 16 649.0 0 16 25 29 649.0 0 29 26 24 649.0 0 24 27 27 649.0 0 27 28 35 649.0 0 35 29 24 649.0 0 24 30 30 649.0 0 30 31 15 649.0 0 15 32 28 649.0 0 28 33 25 649.0 0 25 34 21 649.0 0 21 35 24 649.0 0 24 36 18 649.0 0 18 37 34 649.0 0 34 38 25 649.0 0 25 39 41 649.0 0 41 40 50 649.0 0 50 41 333 649.0 0 333 42 123 649.0 0 123 43 284 649.0 0 284 44 34 649.0 0 34 45 40 649.0 0 40 46 27 649.0 0 27 47 28 649.0 0 28 48 34 649.0 0 34 49 35 649.0 0 35 50 61 649.0 0 61 51 194 649.0 0 194 52 51 649.0 0 51 53 26 649.0 0 26 54 35 649.0 0 35 55 42 649.0 0 42 56 555 649.0 0 555 57 50 649.0 0 50 58 36 649.0 0 36 59 39 649.0 0 39 60 32 649.0 0 32 61 22 649.0 0 22 62 35 649.0 0 35 63 48 649.0 0 48 64 37 649.0 0 37 65 47 649.0 0 47 66 51 649.0 0 51 67 118 649.0 0 118 68 180 649.0 0 180 69 41 649.0 0 41 70 43 649.0 0 43 71 134 649.0 0 134 72 304 649.0 0 304 73 217 649.0 0 217 74 315 649.0 0 315 75 267 649.0 0 267 76 233 649.0 0 233 77 231 649.0 0 231 78 151 649.0 0 151 79 153 649.0 0 153 80 188 649.0 0 188 81 99 649.0 0 99 82 319 649.0 0 319 83 149 649.0 0 149 84 123 649.0 0 123 85 65 649.0 0 65 86 27 649.0 0 27 87 40 649.0 0 40 88 26 649.0 0 26 89 46 649.0 0 46 90 30 649.0 0 30 91 75 649.0 0 75 92 98 649.0 0 98 93 115 649.0 0 115 94 151 649.0 0 151 95 103 649.0 0 103 96 118 649.0 0 118 97 379 649.0 0 379 98 121 649.0 0 121 99 142 649.0 0 142 100 148 649.0 0 148 101 182 649.0 0 182 102 279 649.0 0 279 103 371 649.0 0 371 104 753 649.0 0 753 105 934 649.0 0 934 106 1128 649.0 0 1128 107 977 649.0 0 977 108 705 649.0 0 705 109 594 649.0 0 594 110 501 649.0 0 501 111 645 649.0 0 645 112 603 649.0 0 603 113 739 649.0 0 739 114 636 649.0 0 636 115 632 649.0 0 632 116 559 649.0 0 559 117 445 649.0 0 445 118 541 649.0 0 541 119 571 649.0 0 571 120 657 649.0 0 657 121 470 649.0 0 470 122 477 649.0 0 477 123 429 649.0 0 429 124 623 649.0 0 623 125 662 649.0 0 662 126 590 649.0 0 590 127 387 649.0 0 387 128 376 649.0 0 376 129 373 649.0 0 373 130 382 649.0 0 382 131 381 649.0 0 381 132 575 649.0 0 575 133 2004 649.0 0 2004 134 1953 649.0 0 1953 135 1862 649.0 0 1862 136 454 649.0 0 454 137 396 649.0 0 396 138 450 649.0 0 450 139 329 649.0 0 329 140 438 649.0 0 438 141 332 649.0 0 332 142 288 649.0 0 288 143 345 649.0 0 345 144 365 649.0 0 365 145 373 649.0 0 373 146 401 649.0 0 401 147 419 649.0 0 419 148 373 649.0 0 373 149 339 649.0 0 339 150 317 649.0 0 317 151 271 649.0 0 271 152 280 649.0 0 280 153 322 649.0 0 322 154 367 649.0 0 367 155 372 649.0 0 372 156 340 649.0 0 340 157 349 649.0 0 349 158 416 649.0 0 416 159 558 649.0 0 558 160 530 649.0 0 530 161 613 649.0 0 613 162 1259 649.0 0 1259 163 1199 649.0 0 1199 164 1245 649.0 0 1245 165 479 649.0 0 479 166 436 649.0 0 436 167 394 649.0 0 394 168 341 649.0 0 341 169 371 649.0 0 371 170 370 649.0 0 370 171 418 649.0 0 418 172 403 649.0 0 403 173 455 649.0 0 455 174 288 649.0 0 288 175 336 649.0 0 336 176 264 649.0 0 264 177 297 649.0 0 297 178 352 649.0 0 352 179 389 649.0 0 389 180 403 649.0 0 403 181 383 649.0 0 383 182 391 649.0 0 391 183 582 649.0 0 582 184 448 649.0 0 448 185 457 649.0 0 457 186 343 649.0 0 343 187 372 649.0 0 372 188 338 649.0 0 338 189 407 649.0 0 407 190 401 649.0 0 401 191 444 649.0 0 444 192 394 649.0 0 394 193 393 649.0 0 393 194 276 649.0 0 276 195 279 649.0 0 279 196 287 649.0 0 287 197 277 649.0 0 277 198 329 649.0 0 329 199 304 649.0 0 304 200 338 649.0 0 338 201 330 649.0 0 330 202 292 649.0 0 292 203 317 649.0 0 317 204 285 649.0 0 285 205 333 649.0 0 333 206 368 649.0 0 368 207 432 649.0 0 432 208 525 649.0 0 525 209 453 649.0 0 453 210 427 649.0 0 427 211 837 649.0 0 837 212 1496 649.0 0 1496 213 3495 649.0 0 3495 214 4365 649.0 0 4365 215 6567 649.0 0 6567 216 702 649.0 0 702 217 1254 649.0 0 1254 218 496 649.0 0 496 219 568 649.0 0 568 220 363 649.0 0 363 221 404 649.0 0 404 222 376 649.0 0 376 223 427 649.0 0 427 224 445 649.0 0 445 225 772 649.0 0 772 226 916 649.0 0 916 227 963 649.0 0 963 228 564 649.0 0 564 229 682 649.0 0 682 230 463 649.0 0 463 231 461 649.0 0 461 232 340 649.0 0 340 233 284 649.0 0 284 234 351 649.0 0 351 235 373 649.0 0 373 236 392 649.0 0 392 237 448 649.0 0 448 238 360 649.0 0 360 239 340 649.0 0 340 240 353 649.0 0 353 241 345 649.0 0 345 242 365 649.0 0 365 243 309 649.0 0 309 244 285 649.0 0 285 245 313 649.0 0 313 246 347 649.0 0 347 247 439 649.0 0 439 248 467 649.0 0 467 249 471 649.0 0 471 250 792 649.0 0 792 251 854 649.0 0 854 252 799 649.0 0 799 253 596 649.0 0 596 254 788 649.0 0 788 255 660 649.0 0 660 256 572 649.0 0 572 257 469 649.0 0 469 258 456 649.0 0 456 259 407 649.0 0 407 260 355 649.0 0 355 261 336 649.0 0 336 262 383 649.0 0 383 263 525 649.0 0 525 264 582 649.0 0 582 265 604 649.0 0 604 266 569 649.0 0 569 267 412 649.0 0 412 268 514 649.0 0 514 269 544 649.0 0 544 270 633 649.0 0 633 271 592 649.0 0 592 272 485 649.0 0 485 273 525 649.0 0 525 274 495 649.0 0 495 275 495 649.0 0 495 276 535 649.0 0 535 277 551 649.0 0 551 278 562 649.0 0 562 279 529 649.0 0 529 280 432 649.0 0 432 281 478 649.0 0 478 282 580 649.0 0 580 283 574 649.0 0 574 284 572 649.0 0 572 285 471 649.0 0 471 286 502 649.0 0 502 287 464 649.0 0 464 288 441 649.0 0 441 289 669 649.0 0 669 290 586 649.0 0 586 291 596 649.0 0 596 292 430 649.0 0 430 293 441 649.0 0 441 294 421 649.0 0 421 295 482 649.0 0 482 296 506 649.0 0 506 297 424 649.0 0 424 298 410 649.0 0 410 299 434 649.0 0 434 300 544 649.0 0 544 301 1091 649.0 0 1091 Finished in 10.90 s (49 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 224,528 Reads with adapters: 79,704 (35.5%) Reads that were too short: 113 (0.1%) Reads written (passing filters): 79,591 (35.4%) Total basepairs processed: 58,228,365 bp Total written (filtered): 21,692,579 bp (37.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 79704 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 40 0.0 3 0 0 33 5 2 19 1367 0.0 3 0 1261 67 31 8 20 76863 0.0 4 73143 3089 471 107 53 21 131 0.0 4 16 104 7 1 3 22 11 0.0 4 1 0 8 1 1 23 98 0.0 4 0 0 0 79 19 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 29 3 0.0 4 1 0 0 0 2 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 36 2 0.0 4 1 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 1 45 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 1 68 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 87 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 0 1 1 121 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 126 12 0.0 4 0 0 0 0 12 127 1028 0.0 4 0 0 0 0 1028 128 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 1 138 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 1 1 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 1 176 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 1 200 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 214 3 0.0 4 0 0 0 0 3 215 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 15 0.0 4 0 0 0 3 12 229 3 0.0 4 0 0 0 0 3 230 2 0.0 4 0 0 0 0 2 232 4 0.0 4 0 0 0 1 3 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 1 0 0 1 241 1 0.0 4 0 0 0 0 1 256 2 0.0 4 0 0 0 0 2 257 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 281 4 0.0 4 0 0 0 0 4 282 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.74 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 79,591 Reads with adapters: 3,867 (4.9%) Reads written (passing filters): 79,591 (100.0%) Total basepairs processed: 21,692,579 bp Total written (filtered): 21,632,593 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3867 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 30.9% C: 12.2% G: 54.7% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 363 1243.6 0 363 4 43 310.9 0 43 5 2 77.7 0 2 7 2 4.9 0 2 8 1 1.2 0 1 9 13 0.3 0 0 13 10 54 0.1 1 2 52 11 1241 0.0 1 53 1188 12 1096 0.0 1 25 1071 13 61 0.0 1 2 59 14 2 0.0 1 0 2 15 5 0.0 1 1 4 16 1 0.0 1 0 1 19 1 0.0 1 0 1 20 830 0.0 1 567 263 21 42 0.0 1 32 10 23 6 0.0 1 5 1 24 31 0.0 1 23 8 25 8 0.0 1 4 4 58 2 0.0 1 1 1 86 1 0.0 1 1 95 2 0.0 1 2 104 1 0.0 1 1 125 1 0.0 1 1 144 1 0.0 1 1 152 18 0.0 1 15 3 162 2 0.0 1 0 2 171 1 0.0 1 0 1 209 1 0.0 1 1 210 27 0.0 1 23 4 211 2 0.0 1 2 225 3 0.0 1 2 1 242 1 0.0 1 1 259 1 0.0 1 1 265 1 0.0 1 0 1