This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.87 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 354,056 (13.3%) Reads that were too short: 326,781 (12.3%) Reads written (passing filters): 27,275 (1.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 5,326,427 bp (0.7%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 354056 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 392 649.0 0 392 7 3358 649.0 0 3358 8 2670 649.0 0 2670 9 2180 649.0 0 2180 10 40 649.0 0 40 11 32 649.0 0 32 12 24 649.0 0 24 13 37 649.0 0 37 14 38 649.0 0 38 15 24 649.0 0 24 16 29 649.0 0 29 17 45 649.0 0 45 18 51 649.0 0 51 19 21 649.0 0 21 20 80 649.0 0 80 21 74 649.0 0 74 22 84 649.0 0 84 23 26 649.0 0 26 24 31 649.0 0 31 25 27 649.0 0 27 26 45 649.0 0 45 27 20 649.0 0 20 28 21 649.0 0 21 29 37 649.0 0 37 30 23 649.0 0 23 31 29 649.0 0 29 32 19 649.0 0 19 33 71 649.0 0 71 34 47 649.0 0 47 35 38 649.0 0 38 36 32 649.0 0 32 37 26 649.0 0 26 38 34 649.0 0 34 39 32 649.0 0 32 40 25 649.0 0 25 41 28 649.0 0 28 42 34 649.0 0 34 43 28 649.0 0 28 44 27 649.0 0 27 45 76 649.0 0 76 46 25 649.0 0 25 47 39 649.0 0 39 48 36 649.0 0 36 49 28 649.0 0 28 50 26 649.0 0 26 51 34 649.0 0 34 52 26 649.0 0 26 53 32 649.0 0 32 54 17 649.0 0 17 55 23 649.0 0 23 56 26 649.0 0 26 57 29 649.0 0 29 58 31 649.0 0 31 59 15 649.0 0 15 60 29 649.0 0 29 61 24 649.0 0 24 62 23 649.0 0 23 63 51 649.0 0 51 64 59 649.0 0 59 65 42 649.0 0 42 66 51 649.0 0 51 67 60 649.0 0 60 68 38 649.0 0 38 69 25 649.0 0 25 70 22 649.0 0 22 71 33 649.0 0 33 72 29 649.0 0 29 73 42 649.0 0 42 74 37 649.0 0 37 75 37 649.0 0 37 76 33 649.0 0 33 77 48 649.0 0 48 78 38 649.0 0 38 79 44 649.0 0 44 80 31 649.0 0 31 81 47 649.0 0 47 82 37 649.0 0 37 83 30 649.0 0 30 84 39 649.0 0 39 85 43 649.0 0 43 86 59 649.0 0 59 87 120 649.0 0 120 88 73 649.0 0 73 89 48 649.0 0 48 90 61 649.0 0 61 91 73 649.0 0 73 92 27 649.0 0 27 93 80 649.0 0 80 94 105 649.0 0 105 95 107 649.0 0 107 96 50 649.0 0 50 97 54 649.0 0 54 98 50 649.0 0 50 99 65 649.0 0 65 100 23 649.0 0 23 101 35 649.0 0 35 102 36 649.0 0 36 103 29 649.0 0 29 104 26 649.0 0 26 105 30 649.0 0 30 106 39 649.0 0 39 107 33 649.0 0 33 108 34 649.0 0 34 109 26 649.0 0 26 110 24 649.0 0 24 111 34 649.0 0 34 112 51 649.0 0 51 113 38 649.0 0 38 114 34 649.0 0 34 115 18 649.0 0 18 116 36 649.0 0 36 117 29 649.0 0 29 118 44 649.0 0 44 119 36 649.0 0 36 120 42 649.0 0 42 121 38 649.0 0 38 122 41 649.0 0 41 123 35 649.0 0 35 124 89 649.0 0 89 125 212 649.0 0 212 126 269 649.0 0 269 127 210 649.0 0 210 128 41 649.0 0 41 129 46 649.0 0 46 130 99 649.0 0 99 131 306 649.0 0 306 132 297 649.0 0 297 133 182 649.0 0 182 134 126 649.0 0 126 135 48 649.0 0 48 136 55 649.0 0 55 137 41 649.0 0 41 138 40 649.0 0 40 139 36 649.0 0 36 140 35 649.0 0 35 141 139 649.0 0 139 142 47 649.0 0 47 143 74 649.0 0 74 144 49 649.0 0 49 145 96 649.0 0 96 146 65 649.0 0 65 147 49 649.0 0 49 148 51 649.0 0 51 149 44 649.0 0 44 150 49 649.0 0 49 151 35 649.0 0 35 152 37 649.0 0 37 153 56 649.0 0 56 154 80 649.0 0 80 155 68 649.0 0 68 156 54 649.0 0 54 157 32 649.0 0 32 158 23 649.0 0 23 159 23 649.0 0 23 160 32 649.0 0 32 161 24 649.0 0 24 162 27 649.0 0 27 163 28 649.0 0 28 164 31 649.0 0 31 165 58 649.0 0 58 166 47 649.0 0 47 167 75 649.0 0 75 168 55 649.0 0 55 169 48 649.0 0 48 170 41 649.0 0 41 171 43 649.0 0 43 172 88 649.0 0 88 173 171 649.0 0 171 174 666 649.0 0 666 175 264 649.0 0 264 176 311 649.0 0 311 177 67 649.0 0 67 178 53 649.0 0 53 179 215 649.0 0 215 180 288 649.0 0 288 181 326 649.0 0 326 182 124 649.0 0 124 183 99 649.0 0 99 184 53 649.0 0 53 185 216 649.0 0 216 186 192 649.0 0 192 187 195 649.0 0 195 188 71 649.0 0 71 189 80 649.0 0 80 190 111 649.0 0 111 191 146 649.0 0 146 192 534 649.0 0 534 193 1731 649.0 0 1731 194 1039 649.0 0 1039 195 914 649.0 0 914 196 234 649.0 0 234 197 78 649.0 0 78 198 108 649.0 0 108 199 479 649.0 0 479 200 900 649.0 0 900 201 693 649.0 0 693 202 387 649.0 0 387 203 79 649.0 0 79 204 49 649.0 0 49 205 77 649.0 0 77 206 143 649.0 0 143 207 157 649.0 0 157 208 106 649.0 0 106 209 62 649.0 0 62 210 96 649.0 0 96 211 72 649.0 0 72 212 100 649.0 0 100 213 134 649.0 0 134 214 99 649.0 0 99 215 137 649.0 0 137 216 105 649.0 0 105 217 115 649.0 0 115 218 145 649.0 0 145 219 358 649.0 0 358 220 262 649.0 0 262 221 329 649.0 0 329 222 397 649.0 0 397 223 336 649.0 0 336 224 479 649.0 0 479 225 316 649.0 0 316 226 316 649.0 0 316 227 561 649.0 0 561 228 417 649.0 0 417 229 599 649.0 0 599 230 811 649.0 0 811 231 1551 649.0 0 1551 232 16523 649.0 0 16523 233 7417 649.0 0 7417 234 15586 649.0 0 15586 235 1165 649.0 0 1165 236 1519 649.0 0 1519 237 23015 649.0 0 23015 238 14645 649.0 0 14645 239 23088 649.0 0 23088 240 1571 649.0 0 1571 241 4258 649.0 0 4258 242 741 649.0 0 741 243 1911 649.0 0 1911 244 1133 649.0 0 1133 245 1544 649.0 0 1544 246 460 649.0 0 460 247 764 649.0 0 764 248 669 649.0 0 669 249 1118 649.0 0 1118 250 1544 649.0 0 1544 251 2121 649.0 0 2121 252 10413 649.0 0 10413 253 3451 649.0 0 3451 254 4498 649.0 0 4498 255 1742 649.0 0 1742 256 2920 649.0 0 2920 257 49577 649.0 0 49577 258 35900 649.0 0 35900 259 65431 649.0 0 65431 260 18523 649.0 0 18523 261 170 649.0 0 170 262 108 649.0 0 108 263 60 649.0 0 60 264 84 649.0 0 84 265 53 649.0 0 53 266 95 649.0 0 95 267 64 649.0 0 64 268 167 649.0 0 167 269 68 649.0 0 68 270 135 649.0 0 135 271 68 649.0 0 68 272 46 649.0 0 46 273 63 649.0 0 63 274 45 649.0 0 45 275 58 649.0 0 58 276 77 649.0 0 77 277 37 649.0 0 37 278 39 649.0 0 39 279 60 649.0 0 60 280 71 649.0 0 71 281 108 649.0 0 108 282 114 649.0 0 114 283 72 649.0 0 72 284 61 649.0 0 61 285 84 649.0 0 84 286 85 649.0 0 85 287 129 649.0 0 129 288 124 649.0 0 124 289 161 649.0 0 161 290 252 649.0 0 252 291 454 649.0 0 454 292 383 649.0 0 383 293 270 649.0 0 270 294 186 649.0 0 186 295 155 649.0 0 155 296 106 649.0 0 106 297 46 649.0 0 46 298 49 649.0 0 49 299 76 649.0 0 76 300 88 649.0 0 88 301 168 649.0 0 168 Finished in 1.18 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 27,275 Reads with adapters: 2,403 (8.8%) Reads that were too short: 20 (0.1%) Reads written (passing filters): 2,383 (8.7%) Total basepairs processed: 5,326,427 bp Total written (filtered): 645,241 bp (12.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2403 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 0 2 19 57 0.0 3 0 54 2 1 20 2274 0.0 4 2170 74 22 6 2 21 9 0.0 4 3 5 1 22 5 0.0 4 4 0 1 23 8 0.0 4 0 0 0 5 3 41 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 129 25 0.0 4 0 0 0 0 25 147 1 0.0 4 0 0 1 160 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 2,383 Reads with adapters: 525 (22.0%) Reads written (passing filters): 2,383 (100.0%) Total basepairs processed: 645,241 bp Total written (filtered): 638,933 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 525 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 11.0% G: 83.6% T: 3.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 9.3 0 1 8 4 0.0 0 4 9 76 0.0 0 4 72 10 386 0.0 1 32 354 11 50 0.0 1 3 47 12 1 0.0 1 0 1 13 1 0.0 1 0 1 122 1 0.0 1 0 1 169 1 0.0 1 1 208 2 0.0 1 2 223 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.