This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.74 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 380,120 (14.3%) Reads that were too short: 352,634 (13.3%) Reads written (passing filters): 27,486 (1.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 5,508,177 bp (0.7%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 380120 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 364 649.0 0 364 7 3119 649.0 0 3119 8 2634 649.0 0 2634 9 2530 649.0 0 2530 10 53 649.0 0 53 11 49 649.0 0 49 12 37 649.0 0 37 13 50 649.0 0 50 14 52 649.0 0 52 15 33 649.0 0 33 16 54 649.0 0 54 17 59 649.0 0 59 18 30 649.0 0 30 19 53 649.0 0 53 20 74 649.0 0 74 21 51 649.0 0 51 22 53 649.0 0 53 23 41 649.0 0 41 24 47 649.0 0 47 25 42 649.0 0 42 26 40 649.0 0 40 27 37 649.0 0 37 28 37 649.0 0 37 29 54 649.0 0 54 30 39 649.0 0 39 31 33 649.0 0 33 32 17 649.0 0 17 33 42 649.0 0 42 34 36 649.0 0 36 35 44 649.0 0 44 36 54 649.0 0 54 37 37 649.0 0 37 38 42 649.0 0 42 39 36 649.0 0 36 40 35 649.0 0 35 41 35 649.0 0 35 42 41 649.0 0 41 43 30 649.0 0 30 44 33 649.0 0 33 45 33 649.0 0 33 46 48 649.0 0 48 47 59 649.0 0 59 48 53 649.0 0 53 49 33 649.0 0 33 50 45 649.0 0 45 51 49 649.0 0 49 52 35 649.0 0 35 53 61 649.0 0 61 54 37 649.0 0 37 55 45 649.0 0 45 56 46 649.0 0 46 57 55 649.0 0 55 58 38 649.0 0 38 59 35 649.0 0 35 60 42 649.0 0 42 61 52 649.0 0 52 62 41 649.0 0 41 63 66 649.0 0 66 64 58 649.0 0 58 65 35 649.0 0 35 66 63 649.0 0 63 67 64 649.0 0 64 68 39 649.0 0 39 69 53 649.0 0 53 70 30 649.0 0 30 71 54 649.0 0 54 72 27 649.0 0 27 73 52 649.0 0 52 74 47 649.0 0 47 75 58 649.0 0 58 76 44 649.0 0 44 77 54 649.0 0 54 78 64 649.0 0 64 79 61 649.0 0 61 80 37 649.0 0 37 81 71 649.0 0 71 82 60 649.0 0 60 83 44 649.0 0 44 84 37 649.0 0 37 85 52 649.0 0 52 86 52 649.0 0 52 87 49 649.0 0 49 88 63 649.0 0 63 89 71 649.0 0 71 90 68 649.0 0 68 91 62 649.0 0 62 92 56 649.0 0 56 93 67 649.0 0 67 94 75 649.0 0 75 95 39 649.0 0 39 96 42 649.0 0 42 97 56 649.0 0 56 98 52 649.0 0 52 99 62 649.0 0 62 100 32 649.0 0 32 101 44 649.0 0 44 102 81 649.0 0 81 103 45 649.0 0 45 104 54 649.0 0 54 105 49 649.0 0 49 106 59 649.0 0 59 107 44 649.0 0 44 108 49 649.0 0 49 109 38 649.0 0 38 110 46 649.0 0 46 111 48 649.0 0 48 112 41 649.0 0 41 113 41 649.0 0 41 114 43 649.0 0 43 115 53 649.0 0 53 116 46 649.0 0 46 117 60 649.0 0 60 118 50 649.0 0 50 119 42 649.0 0 42 120 45 649.0 0 45 121 69 649.0 0 69 122 67 649.0 0 67 123 53 649.0 0 53 124 83 649.0 0 83 125 184 649.0 0 184 126 211 649.0 0 211 127 116 649.0 0 116 128 62 649.0 0 62 129 58 649.0 0 58 130 84 649.0 0 84 131 318 649.0 0 318 132 265 649.0 0 265 133 202 649.0 0 202 134 104 649.0 0 104 135 62 649.0 0 62 136 70 649.0 0 70 137 69 649.0 0 69 138 66 649.0 0 66 139 56 649.0 0 56 140 59 649.0 0 59 141 136 649.0 0 136 142 59 649.0 0 59 143 105 649.0 0 105 144 67 649.0 0 67 145 95 649.0 0 95 146 56 649.0 0 56 147 75 649.0 0 75 148 72 649.0 0 72 149 68 649.0 0 68 150 73 649.0 0 73 151 87 649.0 0 87 152 76 649.0 0 76 153 97 649.0 0 97 154 90 649.0 0 90 155 106 649.0 0 106 156 84 649.0 0 84 157 89 649.0 0 89 158 64 649.0 0 64 159 68 649.0 0 68 160 75 649.0 0 75 161 72 649.0 0 72 162 72 649.0 0 72 163 83 649.0 0 83 164 80 649.0 0 80 165 81 649.0 0 81 166 95 649.0 0 95 167 128 649.0 0 128 168 125 649.0 0 125 169 95 649.0 0 95 170 86 649.0 0 86 171 77 649.0 0 77 172 128 649.0 0 128 173 211 649.0 0 211 174 424 649.0 0 424 175 259 649.0 0 259 176 144 649.0 0 144 177 86 649.0 0 86 178 84 649.0 0 84 179 161 649.0 0 161 180 169 649.0 0 169 181 204 649.0 0 204 182 140 649.0 0 140 183 139 649.0 0 139 184 102 649.0 0 102 185 261 649.0 0 261 186 246 649.0 0 246 187 255 649.0 0 255 188 120 649.0 0 120 189 128 649.0 0 128 190 132 649.0 0 132 191 141 649.0 0 141 192 421 649.0 0 421 193 1617 649.0 0 1617 194 765 649.0 0 765 195 750 649.0 0 750 196 286 649.0 0 286 197 117 649.0 0 117 198 120 649.0 0 120 199 320 649.0 0 320 200 370 649.0 0 370 201 344 649.0 0 344 202 255 649.0 0 255 203 120 649.0 0 120 204 98 649.0 0 98 205 140 649.0 0 140 206 160 649.0 0 160 207 174 649.0 0 174 208 144 649.0 0 144 209 125 649.0 0 125 210 126 649.0 0 126 211 134 649.0 0 134 212 147 649.0 0 147 213 206 649.0 0 206 214 161 649.0 0 161 215 208 649.0 0 208 216 195 649.0 0 195 217 175 649.0 0 175 218 245 649.0 0 245 219 442 649.0 0 442 220 284 649.0 0 284 221 273 649.0 0 273 222 443 649.0 0 443 223 390 649.0 0 390 224 586 649.0 0 586 225 424 649.0 0 424 226 425 649.0 0 425 227 642 649.0 0 642 228 541 649.0 0 541 229 697 649.0 0 697 230 813 649.0 0 813 231 1597 649.0 0 1597 232 19734 649.0 0 19734 233 8935 649.0 0 8935 234 18990 649.0 0 18990 235 1174 649.0 0 1174 236 1573 649.0 0 1573 237 25743 649.0 0 25743 238 17136 649.0 0 17136 239 24996 649.0 0 24996 240 1442 649.0 0 1442 241 5043 649.0 0 5043 242 803 649.0 0 803 243 2437 649.0 0 2437 244 1319 649.0 0 1319 245 2105 649.0 0 2105 246 580 649.0 0 580 247 836 649.0 0 836 248 802 649.0 0 802 249 1407 649.0 0 1407 250 1714 649.0 0 1714 251 2658 649.0 0 2658 252 12656 649.0 0 12656 253 4183 649.0 0 4183 254 5355 649.0 0 5355 255 1543 649.0 0 1543 256 2560 649.0 0 2560 257 44897 649.0 0 44897 258 36577 649.0 0 36577 259 61743 649.0 0 61743 260 26469 649.0 0 26469 261 180 649.0 0 180 262 110 649.0 0 110 263 84 649.0 0 84 264 104 649.0 0 104 265 89 649.0 0 89 266 118 649.0 0 118 267 126 649.0 0 126 268 178 649.0 0 178 269 100 649.0 0 100 270 147 649.0 0 147 271 119 649.0 0 119 272 107 649.0 0 107 273 88 649.0 0 88 274 98 649.0 0 98 275 87 649.0 0 87 276 87 649.0 0 87 277 106 649.0 0 106 278 95 649.0 0 95 279 104 649.0 0 104 280 109 649.0 0 109 281 125 649.0 0 125 282 155 649.0 0 155 283 135 649.0 0 135 284 108 649.0 0 108 285 118 649.0 0 118 286 113 649.0 0 113 287 94 649.0 0 94 288 104 649.0 0 104 289 194 649.0 0 194 290 283 649.0 0 283 291 634 649.0 0 634 292 783 649.0 0 783 293 493 649.0 0 493 294 306 649.0 0 306 295 167 649.0 0 167 296 141 649.0 0 141 297 120 649.0 0 120 298 114 649.0 0 114 299 107 649.0 0 107 300 127 649.0 0 127 301 197 649.0 0 197 Finished in 1.11 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 27,486 Reads with adapters: 5,858 (21.3%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 5,846 (21.3%) Total basepairs processed: 5,508,177 bp Total written (filtered): 1,604,853 bp (29.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5858 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 14 0.0 3 0 14 17 2492 0.0 3 2427 58 6 1 18 61 0.0 3 4 34 1 22 19 3249 0.0 3 3194 50 3 2 20 13 0.0 3 1 10 0 2 21 3 0.0 3 0 0 3 22 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 60 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 5,846 Reads with adapters: 211 (3.6%) Reads written (passing filters): 5,846 (100.0%) Total basepairs processed: 1,604,853 bp Total written (filtered): 1,604,214 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 211 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.7% C: 11.4% G: 8.5% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 205 91.3 0 205 4 6 22.8 0 6