This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.31 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 69,135 (2.6%) Reads that were too short: 16,293 (0.6%) Reads written (passing filters): 52,842 (2.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 13,128,667 bp (1.6%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 69135 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 571 649.0 0 571 7 14490 649.0 0 14490 8 423 649.0 0 423 9 17079 649.0 0 17079 10 29 649.0 0 29 11 7 649.0 0 7 12 17 649.0 0 17 13 17 649.0 0 17 14 17 649.0 0 17 15 17 649.0 0 17 16 18 649.0 0 18 17 96 649.0 0 96 18 45 649.0 0 45 19 8 649.0 0 8 20 20 649.0 0 20 21 18 649.0 0 18 22 35 649.0 0 35 23 47 649.0 0 47 24 14 649.0 0 14 25 39 649.0 0 39 26 255 649.0 0 255 27 116 649.0 0 116 28 329 649.0 0 329 29 150 649.0 0 150 30 278 649.0 0 278 31 203 649.0 0 203 32 16 649.0 0 16 33 16 649.0 0 16 34 14 649.0 0 14 35 23 649.0 0 23 36 21 649.0 0 21 37 12 649.0 0 12 38 21 649.0 0 21 39 25 649.0 0 25 40 18 649.0 0 18 41 10 649.0 0 10 42 17 649.0 0 17 43 16 649.0 0 16 44 19 649.0 0 19 45 28 649.0 0 28 46 20 649.0 0 20 47 15 649.0 0 15 48 12 649.0 0 12 49 13 649.0 0 13 50 16 649.0 0 16 51 38 649.0 0 38 52 15 649.0 0 15 53 19 649.0 0 19 54 18 649.0 0 18 55 19 649.0 0 19 56 33 649.0 0 33 57 23 649.0 0 23 58 21 649.0 0 21 59 29 649.0 0 29 60 19 649.0 0 19 61 14 649.0 0 14 62 24 649.0 0 24 63 37 649.0 0 37 64 129 649.0 0 129 65 44 649.0 0 44 66 23 649.0 0 23 67 29 649.0 0 29 68 59 649.0 0 59 69 542 649.0 0 542 70 166 649.0 0 166 71 450 649.0 0 450 72 37 649.0 0 37 73 41 649.0 0 41 74 14 649.0 0 14 75 56 649.0 0 56 76 34 649.0 0 34 77 157 649.0 0 157 78 49 649.0 0 49 79 86 649.0 0 86 80 42 649.0 0 42 81 37 649.0 0 37 82 37 649.0 0 37 83 66 649.0 0 66 84 66 649.0 0 66 85 41 649.0 0 41 86 75 649.0 0 75 87 222 649.0 0 222 88 58 649.0 0 58 89 58 649.0 0 58 90 151 649.0 0 151 91 71 649.0 0 71 92 38 649.0 0 38 93 118 649.0 0 118 94 38 649.0 0 38 95 32 649.0 0 32 96 96 649.0 0 96 97 107 649.0 0 107 98 51 649.0 0 51 99 75 649.0 0 75 100 29 649.0 0 29 101 42 649.0 0 42 102 18 649.0 0 18 103 10 649.0 0 10 104 32 649.0 0 32 105 28 649.0 0 28 106 21 649.0 0 21 107 22 649.0 0 22 108 22 649.0 0 22 109 17 649.0 0 17 110 75 649.0 0 75 111 51 649.0 0 51 112 101 649.0 0 101 113 42 649.0 0 42 114 148 649.0 0 148 115 226 649.0 0 226 116 250 649.0 0 250 117 32 649.0 0 32 118 18 649.0 0 18 119 25 649.0 0 25 120 58 649.0 0 58 121 28 649.0 0 28 122 36 649.0 0 36 123 38 649.0 0 38 124 32 649.0 0 32 125 32 649.0 0 32 126 33 649.0 0 33 127 45 649.0 0 45 128 80 649.0 0 80 129 63 649.0 0 63 130 56 649.0 0 56 131 79 649.0 0 79 132 81 649.0 0 81 133 141 649.0 0 141 134 148 649.0 0 148 135 115 649.0 0 115 136 103 649.0 0 103 137 230 649.0 0 230 138 326 649.0 0 326 139 1023 649.0 0 1023 140 449 649.0 0 449 141 140 649.0 0 140 142 219 649.0 0 219 143 339 649.0 0 339 144 160 649.0 0 160 145 100 649.0 0 100 146 116 649.0 0 116 147 120 649.0 0 120 148 1502 649.0 0 1502 149 1526 649.0 0 1526 150 1230 649.0 0 1230 151 990 649.0 0 990 152 220 649.0 0 220 153 53 649.0 0 53 154 98 649.0 0 98 155 75 649.0 0 75 156 65 649.0 0 65 157 48 649.0 0 48 158 71 649.0 0 71 159 49 649.0 0 49 160 117 649.0 0 117 161 35 649.0 0 35 162 39 649.0 0 39 163 32 649.0 0 32 164 30 649.0 0 30 165 24 649.0 0 24 166 36 649.0 0 36 167 37 649.0 0 37 168 42 649.0 0 42 169 39 649.0 0 39 170 38 649.0 0 38 171 41 649.0 0 41 172 39 649.0 0 39 173 84 649.0 0 84 174 50 649.0 0 50 175 50 649.0 0 50 176 48 649.0 0 48 177 46 649.0 0 46 178 43 649.0 0 43 179 55 649.0 0 55 180 67 649.0 0 67 181 37 649.0 0 37 182 46 649.0 0 46 183 57 649.0 0 57 184 274 649.0 0 274 185 91 649.0 0 91 186 201 649.0 0 201 187 56 649.0 0 56 188 49 649.0 0 49 189 128 649.0 0 128 190 119 649.0 0 119 191 191 649.0 0 191 192 103 649.0 0 103 193 106 649.0 0 106 194 73 649.0 0 73 195 58 649.0 0 58 196 59 649.0 0 59 197 77 649.0 0 77 198 39 649.0 0 39 199 81 649.0 0 81 200 48 649.0 0 48 201 57 649.0 0 57 202 73 649.0 0 73 203 52 649.0 0 52 204 77 649.0 0 77 205 58 649.0 0 58 206 57 649.0 0 57 207 65 649.0 0 65 208 96 649.0 0 96 209 261 649.0 0 261 210 276 649.0 0 276 211 194 649.0 0 194 212 111 649.0 0 111 213 110 649.0 0 110 214 70 649.0 0 70 215 154 649.0 0 154 216 71 649.0 0 71 217 70 649.0 0 70 218 130 649.0 0 130 219 66 649.0 0 66 220 66 649.0 0 66 221 71 649.0 0 71 222 68 649.0 0 68 223 103 649.0 0 103 224 53 649.0 0 53 225 118 649.0 0 118 226 57 649.0 0 57 227 60 649.0 0 60 228 80 649.0 0 80 229 76 649.0 0 76 230 106 649.0 0 106 231 69 649.0 0 69 232 104 649.0 0 104 233 279 649.0 0 279 234 311 649.0 0 311 235 376 649.0 0 376 236 249 649.0 0 249 237 98 649.0 0 98 238 106 649.0 0 106 239 91 649.0 0 91 240 87 649.0 0 87 241 101 649.0 0 101 242 118 649.0 0 118 243 181 649.0 0 181 244 151 649.0 0 151 245 128 649.0 0 128 246 113 649.0 0 113 247 137 649.0 0 137 248 208 649.0 0 208 249 154 649.0 0 154 250 147 649.0 0 147 251 100 649.0 0 100 252 115 649.0 0 115 253 578 649.0 0 578 254 436 649.0 0 436 255 666 649.0 0 666 256 155 649.0 0 155 257 94 649.0 0 94 258 78 649.0 0 78 259 97 649.0 0 97 260 97 649.0 0 97 261 104 649.0 0 104 262 98 649.0 0 98 263 91 649.0 0 91 264 93 649.0 0 93 265 94 649.0 0 94 266 119 649.0 0 119 267 131 649.0 0 131 268 307 649.0 0 307 269 142 649.0 0 142 270 197 649.0 0 197 271 120 649.0 0 120 272 156 649.0 0 156 273 482 649.0 0 482 274 356 649.0 0 356 275 298 649.0 0 298 276 141 649.0 0 141 277 77 649.0 0 77 278 170 649.0 0 170 279 116 649.0 0 116 280 229 649.0 0 229 281 156 649.0 0 156 282 96 649.0 0 96 283 147 649.0 0 147 284 118 649.0 0 118 285 134 649.0 0 134 286 132 649.0 0 132 287 128 649.0 0 128 288 150 649.0 0 150 289 236 649.0 0 236 290 111 649.0 0 111 291 132 649.0 0 132 292 231 649.0 0 231 293 159 649.0 0 159 294 193 649.0 0 193 295 213 649.0 0 213 296 181 649.0 0 181 297 246 649.0 0 246 298 218 649.0 0 218 299 320 649.0 0 320 300 414 649.0 0 414 301 584 649.0 0 584 Finished in 2.72 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 52,842 Reads with adapters: 15,049 (28.5%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 15,025 (28.4%) Total basepairs processed: 13,128,667 bp Total written (filtered): 4,084,599 bp (31.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15049 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 4 1 19 194 0.0 3 0 178 7 8 1 20 14406 0.0 4 13615 659 95 29 8 21 84 0.0 4 47 31 4 1 1 22 6 0.0 4 3 1 0 1 1 23 31 0.0 4 1 0 0 21 9 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 108 6 0.0 4 0 0 0 0 6 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 280 0.0 4 0 0 0 0 280 128 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 177 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 2 3 235 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 15,025 Reads with adapters: 923 (6.1%) Reads written (passing filters): 15,025 (100.0%) Total basepairs processed: 4,084,599 bp Total written (filtered): 4,071,076 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 923 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.6% C: 13.5% G: 76.6% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 63 234.8 0 63 4 4 58.7 0 4 5 1 14.7 0 1 9 3 0.1 0 1 2 10 6 0.0 1 1 5 11 593 0.0 1 20 573 12 226 0.0 1 4 222 13 4 0.0 1 0 4 14 1 0.0 1 0 1 15 2 0.0 1 0 2 60 1 0.0 1 0 1 152 2 0.0 1 2 171 1 0.0 1 1 210 16 0.0 1 11 5