This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.96 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 153,402 (5.8%) Reads that were too short: 21,149 (0.8%) Reads written (passing filters): 132,253 (5.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 36,845,410 bp (4.6%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 153402 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5125 649.0 0 5125 7 1631 649.0 0 1631 8 58647 649.0 0 58647 9 48458 649.0 0 48458 10 104 649.0 0 104 11 11 649.0 0 11 12 6 649.0 0 6 13 7 649.0 0 7 14 4 649.0 0 4 15 8 649.0 0 8 16 5 649.0 0 5 17 8 649.0 0 8 18 13 649.0 0 13 19 5 649.0 0 5 20 7 649.0 0 7 21 8 649.0 0 8 22 4 649.0 0 4 23 1 649.0 0 1 24 6 649.0 0 6 25 14 649.0 0 14 26 28 649.0 0 28 27 39 649.0 0 39 28 31 649.0 0 31 29 8 649.0 0 8 30 8 649.0 0 8 31 19 649.0 0 19 32 37 649.0 0 37 33 22 649.0 0 22 34 22 649.0 0 22 35 15 649.0 0 15 36 11 649.0 0 11 37 9 649.0 0 9 38 22 649.0 0 22 39 32 649.0 0 32 40 49 649.0 0 49 41 36 649.0 0 36 42 44 649.0 0 44 43 49 649.0 0 49 44 21 649.0 0 21 45 13 649.0 0 13 46 24 649.0 0 24 47 17 649.0 0 17 48 26 649.0 0 26 49 19 649.0 0 19 50 17 649.0 0 17 51 16 649.0 0 16 52 56 649.0 0 56 53 37 649.0 0 37 54 23 649.0 0 23 55 19 649.0 0 19 56 25 649.0 0 25 57 38 649.0 0 38 58 191 649.0 0 191 59 224 649.0 0 224 60 1453 649.0 0 1453 61 1178 649.0 0 1178 62 945 649.0 0 945 63 24 649.0 0 24 64 28 649.0 0 28 65 30 649.0 0 30 66 267 649.0 0 267 67 163 649.0 0 163 68 88 649.0 0 88 69 17 649.0 0 17 70 24 649.0 0 24 71 26 649.0 0 26 72 27 649.0 0 27 73 25 649.0 0 25 74 23 649.0 0 23 75 27 649.0 0 27 76 56 649.0 0 56 77 287 649.0 0 287 78 121 649.0 0 121 79 155 649.0 0 155 80 21 649.0 0 21 81 75 649.0 0 75 82 101 649.0 0 101 83 61 649.0 0 61 84 238 649.0 0 238 85 226 649.0 0 226 86 27 649.0 0 27 87 38 649.0 0 38 88 39 649.0 0 39 89 44 649.0 0 44 90 28 649.0 0 28 91 105 649.0 0 105 92 43 649.0 0 43 93 113 649.0 0 113 94 89 649.0 0 89 95 53 649.0 0 53 96 32 649.0 0 32 97 64 649.0 0 64 98 53 649.0 0 53 99 42 649.0 0 42 100 28 649.0 0 28 101 19 649.0 0 19 102 19 649.0 0 19 103 22 649.0 0 22 104 40 649.0 0 40 105 25 649.0 0 25 106 25 649.0 0 25 107 39 649.0 0 39 108 54 649.0 0 54 109 33 649.0 0 33 110 27 649.0 0 27 111 225 649.0 0 225 112 114 649.0 0 114 113 182 649.0 0 182 114 1055 649.0 0 1055 115 430 649.0 0 430 116 1681 649.0 0 1681 117 179 649.0 0 179 118 115 649.0 0 115 119 48 649.0 0 48 120 30 649.0 0 30 121 26 649.0 0 26 122 74 649.0 0 74 123 68 649.0 0 68 124 64 649.0 0 64 125 55 649.0 0 55 126 112 649.0 0 112 127 50 649.0 0 50 128 35 649.0 0 35 129 45 649.0 0 45 130 53 649.0 0 53 131 70 649.0 0 70 132 43 649.0 0 43 133 67 649.0 0 67 134 29 649.0 0 29 135 51 649.0 0 51 136 61 649.0 0 61 137 83 649.0 0 83 138 46 649.0 0 46 139 91 649.0 0 91 140 68 649.0 0 68 141 49 649.0 0 49 142 34 649.0 0 34 143 104 649.0 0 104 144 89 649.0 0 89 145 182 649.0 0 182 146 39 649.0 0 39 147 69 649.0 0 69 148 186 649.0 0 186 149 65 649.0 0 65 150 60 649.0 0 60 151 36 649.0 0 36 152 41 649.0 0 41 153 37 649.0 0 37 154 44 649.0 0 44 155 36 649.0 0 36 156 63 649.0 0 63 157 48 649.0 0 48 158 36 649.0 0 36 159 52 649.0 0 52 160 57 649.0 0 57 161 82 649.0 0 82 162 50 649.0 0 50 163 65 649.0 0 65 164 72 649.0 0 72 165 168 649.0 0 168 166 110 649.0 0 110 167 152 649.0 0 152 168 41 649.0 0 41 169 64 649.0 0 64 170 55 649.0 0 55 171 65 649.0 0 65 172 46 649.0 0 46 173 46 649.0 0 46 174 42 649.0 0 42 175 37 649.0 0 37 176 62 649.0 0 62 177 59 649.0 0 59 178 61 649.0 0 61 179 172 649.0 0 172 180 127 649.0 0 127 181 121 649.0 0 121 182 54 649.0 0 54 183 50 649.0 0 50 184 101 649.0 0 101 185 121 649.0 0 121 186 92 649.0 0 92 187 64 649.0 0 64 188 74 649.0 0 74 189 168 649.0 0 168 190 139 649.0 0 139 191 287 649.0 0 287 192 166 649.0 0 166 193 92 649.0 0 92 194 55 649.0 0 55 195 60 649.0 0 60 196 63 649.0 0 63 197 61 649.0 0 61 198 75 649.0 0 75 199 48 649.0 0 48 200 54 649.0 0 54 201 49 649.0 0 49 202 48 649.0 0 48 203 170 649.0 0 170 204 155 649.0 0 155 205 116 649.0 0 116 206 56 649.0 0 56 207 86 649.0 0 86 208 175 649.0 0 175 209 98 649.0 0 98 210 148 649.0 0 148 211 53 649.0 0 53 212 143 649.0 0 143 213 91 649.0 0 91 214 112 649.0 0 112 215 69 649.0 0 69 216 117 649.0 0 117 217 147 649.0 0 147 218 142 649.0 0 142 219 81 649.0 0 81 220 61 649.0 0 61 221 63 649.0 0 63 222 67 649.0 0 67 223 69 649.0 0 69 224 122 649.0 0 122 225 176 649.0 0 176 226 136 649.0 0 136 227 87 649.0 0 87 228 74 649.0 0 74 229 81 649.0 0 81 230 51 649.0 0 51 231 96 649.0 0 96 232 115 649.0 0 115 233 109 649.0 0 109 234 107 649.0 0 107 235 145 649.0 0 145 236 340 649.0 0 340 237 470 649.0 0 470 238 357 649.0 0 357 239 280 649.0 0 280 240 79 649.0 0 79 241 83 649.0 0 83 242 125 649.0 0 125 243 117 649.0 0 117 244 240 649.0 0 240 245 3099 649.0 0 3099 246 1502 649.0 0 1502 247 3407 649.0 0 3407 248 115 649.0 0 115 249 72 649.0 0 72 250 64 649.0 0 64 251 69 649.0 0 69 252 65 649.0 0 65 253 83 649.0 0 83 254 124 649.0 0 124 255 93 649.0 0 93 256 89 649.0 0 89 257 68 649.0 0 68 258 83 649.0 0 83 259 105 649.0 0 105 260 195 649.0 0 195 261 306 649.0 0 306 262 167 649.0 0 167 263 135 649.0 0 135 264 149 649.0 0 149 265 512 649.0 0 512 266 334 649.0 0 334 267 291 649.0 0 291 268 98 649.0 0 98 269 68 649.0 0 68 270 251 649.0 0 251 271 220 649.0 0 220 272 356 649.0 0 356 273 143 649.0 0 143 274 66 649.0 0 66 275 123 649.0 0 123 276 147 649.0 0 147 277 127 649.0 0 127 278 121 649.0 0 121 279 178 649.0 0 178 280 142 649.0 0 142 281 129 649.0 0 129 282 104 649.0 0 104 283 71 649.0 0 71 284 97 649.0 0 97 285 122 649.0 0 122 286 151 649.0 0 151 287 109 649.0 0 109 288 87 649.0 0 87 289 71 649.0 0 71 290 73 649.0 0 73 291 82 649.0 0 82 292 101 649.0 0 101 293 135 649.0 0 135 294 140 649.0 0 140 295 97 649.0 0 97 296 111 649.0 0 111 297 134 649.0 0 134 298 147 649.0 0 147 299 122 649.0 0 122 300 153 649.0 0 153 301 89 649.0 0 89 Finished in 6.85 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 132,253 Reads with adapters: 57,217 (43.3%) Reads that were too short: 44 (0.0%) Reads written (passing filters): 57,173 (43.2%) Total basepairs processed: 36,845,410 bp Total written (filtered): 15,331,893 bp (41.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 57217 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 7 0.0 3 0 0 0 2 5 18 36 0.0 3 0 0 22 10 4 19 727 0.0 3 0 655 42 18 12 20 49622 0.0 4 47085 2099 328 87 23 21 193 0.0 4 35 138 11 7 2 22 4223 0.0 4 4034 149 31 7 2 23 200 0.0 4 6 10 0 147 37 24 7 0.0 4 3 0 1 1 2 25 17 0.0 4 0 0 0 11 6 27 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 43 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 1 63 1 0.0 4 0 1 67 1 0.0 4 0 0 0 1 70 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 1 0 0 1 99 2 0.0 4 0 0 0 1 1 103 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 125 5 0.0 4 0 0 0 0 5 126 28 0.0 4 1 0 0 0 27 127 2080 0.0 4 0 0 0 1 2079 128 5 0.0 4 0 0 0 0 5 130 3 0.0 4 0 0 0 0 3 135 1 0.0 4 0 0 0 1 136 1 0.0 4 0 0 1 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 189 1 0.0 4 0 0 0 1 191 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 1 202 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 2 0.0 4 0 0 0 0 2 250 1 0.0 4 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.06 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 57,173 Reads with adapters: 13,228 (23.1%) Reads written (passing filters): 57,173 (100.0%) Total basepairs processed: 15,331,893 bp Total written (filtered): 15,182,481 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13228 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 7.5% G: 87.7% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 32 893.3 0 32 4 9 223.3 0 9 7 1 3.5 0 1 8 5 0.9 0 5 9 3837 0.2 0 408 3429 10 7728 0.1 1 554 7174 11 1430 0.0 1 57 1373 12 59 0.0 1 2 57 13 24 0.0 1 0 24 14 1 0.0 1 0 1 15 1 0.0 1 0 1 139 1 0.0 1 1 150 3 0.0 1 2 1 151 1 0.0 1 1 152 1 0.0 1 1 169 6 0.0 1 6 177 1 0.0 1 1 189 2 0.0 1 1 1 203 1 0.0 1 0 1 208 63 0.0 1 50 13 209 4 0.0 1 4 210 2 0.0 1 2 223 15 0.0 1 14 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.