This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 56.58 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 105,230 (4.7%) Reads that were too short: 40,668 (1.8%) Reads written (passing filters): 64,562 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 17,090,693 bp (2.5%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 105230 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 347 546.6 0 347 7 20730 546.6 0 20730 8 27532 546.6 0 27532 9 50 546.6 0 50 10 49 546.6 0 49 11 86 546.6 0 86 12 33 546.6 0 33 13 48 546.6 0 48 14 34 546.6 0 34 15 56 546.6 0 56 16 41 546.6 0 41 17 45 546.6 0 45 18 40 546.6 0 40 19 33 546.6 0 33 20 30 546.6 0 30 21 35 546.6 0 35 22 28 546.6 0 28 23 32 546.6 0 32 24 40 546.6 0 40 25 55 546.6 0 55 26 28 546.6 0 28 27 32 546.6 0 32 28 39 546.6 0 39 29 42 546.6 0 42 30 27 546.6 0 27 31 54 546.6 0 54 32 48 546.6 0 48 33 34 546.6 0 34 34 36 546.6 0 36 35 44 546.6 0 44 36 30 546.6 0 30 37 42 546.6 0 42 38 45 546.6 0 45 39 37 546.6 0 37 40 38 546.6 0 38 41 40 546.6 0 40 42 58 546.6 0 58 43 33 546.6 0 33 44 29 546.6 0 29 45 47 546.6 0 47 46 34 546.6 0 34 47 35 546.6 0 35 48 42 546.6 0 42 49 50 546.6 0 50 50 37 546.6 0 37 51 35 546.6 0 35 52 46 546.6 0 46 53 56 546.6 0 56 54 68 546.6 0 68 55 33 546.6 0 33 56 40 546.6 0 40 57 40 546.6 0 40 58 26 546.6 0 26 59 161 546.6 0 161 60 65 546.6 0 65 61 48 546.6 0 48 62 37 546.6 0 37 63 48 546.6 0 48 64 60 546.6 0 60 65 55 546.6 0 55 66 50 546.6 0 50 67 37 546.6 0 37 68 39 546.6 0 39 69 44 546.6 0 44 70 46 546.6 0 46 71 70 546.6 0 70 72 116 546.6 0 116 73 84 546.6 0 84 74 102 546.6 0 102 75 61 546.6 0 61 76 56 546.6 0 56 77 61 546.6 0 61 78 85 546.6 0 85 79 90 546.6 0 90 80 93 546.6 0 93 81 126 546.6 0 126 82 110 546.6 0 110 83 99 546.6 0 99 84 109 546.6 0 109 85 89 546.6 0 89 86 114 546.6 0 114 87 103 546.6 0 103 88 89 546.6 0 89 89 57 546.6 0 57 90 56 546.6 0 56 91 58 546.6 0 58 92 62 546.6 0 62 93 64 546.6 0 64 94 56 546.6 0 56 95 70 546.6 0 70 96 78 546.6 0 78 97 57 546.6 0 57 98 65 546.6 0 65 99 96 546.6 0 96 100 55 546.6 0 55 101 50 546.6 0 50 102 88 546.6 0 88 103 92 546.6 0 92 104 107 546.6 0 107 105 72 546.6 0 72 106 80 546.6 0 80 107 84 546.6 0 84 108 58 546.6 0 58 109 74 546.6 0 74 110 72 546.6 0 72 111 81 546.6 0 81 112 64 546.6 0 64 113 49 546.6 0 49 114 72 546.6 0 72 115 64 546.6 0 64 116 83 546.6 0 83 117 80 546.6 0 80 118 82 546.6 0 82 119 68 546.6 0 68 120 53 546.6 0 53 121 87 546.6 0 87 122 66 546.6 0 66 123 88 546.6 0 88 124 211 546.6 0 211 125 206 546.6 0 206 126 337 546.6 0 337 127 61 546.6 0 61 128 80 546.6 0 80 129 65 546.6 0 65 130 89 546.6 0 89 131 98 546.6 0 98 132 102 546.6 0 102 133 144 546.6 0 144 134 103 546.6 0 103 135 102 546.6 0 102 136 128 546.6 0 128 137 90 546.6 0 90 138 70 546.6 0 70 139 69 546.6 0 69 140 79 546.6 0 79 141 72 546.6 0 72 142 79 546.6 0 79 143 85 546.6 0 85 144 72 546.6 0 72 145 83 546.6 0 83 146 115 546.6 0 115 147 260 546.6 0 260 148 104 546.6 0 104 149 194 546.6 0 194 150 91 546.6 0 91 151 70 546.6 0 70 152 68 546.6 0 68 153 76 546.6 0 76 154 81 546.6 0 81 155 93 546.6 0 93 156 198 546.6 0 198 157 82 546.6 0 82 158 147 546.6 0 147 159 116 546.6 0 116 160 116 546.6 0 116 161 86 546.6 0 86 162 107 546.6 0 107 163 132 546.6 0 132 164 87 546.6 0 87 165 76 546.6 0 76 166 90 546.6 0 90 167 105 546.6 0 105 168 79 546.6 0 79 169 255 546.6 0 255 170 319 546.6 0 319 171 371 546.6 0 371 172 141 546.6 0 141 173 101 546.6 0 101 174 99 546.6 0 99 175 81 546.6 0 81 176 61 546.6 0 61 177 66 546.6 0 66 178 91 546.6 0 91 179 93 546.6 0 93 180 91 546.6 0 91 181 87 546.6 0 87 182 140 546.6 0 140 183 109 546.6 0 109 184 88 546.6 0 88 185 117 546.6 0 117 186 98 546.6 0 98 187 144 546.6 0 144 188 150 546.6 0 150 189 105 546.6 0 105 190 103 546.6 0 103 191 79 546.6 0 79 192 122 546.6 0 122 193 109 546.6 0 109 194 115 546.6 0 115 195 143 546.6 0 143 196 96 546.6 0 96 197 76 546.6 0 76 198 112 546.6 0 112 199 106 546.6 0 106 200 95 546.6 0 95 201 97 546.6 0 97 202 94 546.6 0 94 203 185 546.6 0 185 204 124 546.6 0 124 205 200 546.6 0 200 206 77 546.6 0 77 207 67 546.6 0 67 208 89 546.6 0 89 209 86 546.6 0 86 210 83 546.6 0 83 211 95 546.6 0 95 212 109 546.6 0 109 213 153 546.6 0 153 214 597 546.6 0 597 215 1509 546.6 0 1509 216 845 546.6 0 845 217 287 546.6 0 287 218 128 546.6 0 128 219 156 546.6 0 156 220 97 546.6 0 97 221 74 546.6 0 74 222 117 546.6 0 117 223 147 546.6 0 147 224 178 546.6 0 178 225 394 546.6 0 394 226 1888 546.6 0 1888 227 6915 546.6 0 6915 228 2441 546.6 0 2441 229 2676 546.6 0 2676 230 894 546.6 0 894 231 2306 546.6 0 2306 232 1041 546.6 0 1041 233 423 546.6 0 423 234 76 546.6 0 76 235 75 546.6 0 75 236 73 546.6 0 73 237 163 546.6 0 163 238 137 546.6 0 137 239 161 546.6 0 161 240 99 546.6 0 99 241 114 546.6 0 114 242 82 546.6 0 82 243 65 546.6 0 65 244 112 546.6 0 112 245 89 546.6 0 89 246 87 546.6 0 87 247 105 546.6 0 105 248 178 546.6 0 178 249 166 546.6 0 166 250 129 546.6 0 129 251 82 546.6 0 82 252 88 546.6 0 88 253 89 546.6 0 89 254 99 546.6 0 99 255 111 546.6 0 111 256 108 546.6 0 108 257 142 546.6 0 142 258 93 546.6 0 93 259 136 546.6 0 136 260 141 546.6 0 141 261 212 546.6 0 212 262 134 546.6 0 134 263 191 546.6 0 191 264 819 546.6 0 819 265 1140 546.6 0 1140 266 1586 546.6 0 1586 267 821 546.6 0 821 268 653 546.6 0 653 269 292 546.6 0 292 270 465 546.6 0 465 271 134 546.6 0 134 272 101 546.6 0 101 273 204 546.6 0 204 274 331 546.6 0 331 275 270 546.6 0 270 276 244 546.6 0 244 277 132 546.6 0 132 278 168 546.6 0 168 279 177 546.6 0 177 280 183 546.6 0 183 281 224 546.6 0 224 282 141 546.6 0 141 283 110 546.6 0 110 284 130 546.6 0 130 285 134 546.6 0 134 286 165 546.6 0 165 287 171 546.6 0 171 288 156 546.6 0 156 289 124 546.6 0 124 290 143 546.6 0 143 291 655 546.6 0 655 292 615 546.6 0 615 293 1302 546.6 0 1302 294 235 546.6 0 235 295 68 546.6 0 68 296 73 546.6 0 73 297 91 546.6 0 91 298 83 546.6 0 83 299 97 546.6 0 97 300 96 546.6 0 96 301 123 546.6 0 123 Finished in 2.95 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 64,562 Reads with adapters: 20,471 (31.7%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 20,454 (31.7%) Total basepairs processed: 17,090,693 bp Total written (filtered): 5,662,770 bp (33.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20471 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 157 0.0 3 0 153 4 17 20171 0.0 3 19797 362 11 1 18 42 0.0 3 5 35 2 19 8 0.0 3 0 0 5 3 20 56 0.0 3 1 0 0 55 22 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 1 40 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 1 1 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 1 118 2 0.0 3 1 0 1 123 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 1 213 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.71 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 20,454 Reads with adapters: 103 (0.5%) Reads written (passing filters): 20,454 (100.0%) Total basepairs processed: 5,662,770 bp Total written (filtered): 5,662,457 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 103 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.3% C: 21.4% G: 15.5% T: 7.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 99 319.6 0 99 4 4 79.9 0 4