This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.13 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 118,193 (5.3%) Reads that were too short: 7,634 (0.3%) Reads written (passing filters): 110,559 (4.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 30,363,801 bp (4.5%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 118193 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1933 546.6 0 1933 7 40582 546.6 0 40582 8 1155 546.6 0 1155 9 49279 546.6 0 49279 10 47 546.6 0 47 11 7 546.6 0 7 12 1 546.6 0 1 13 3 546.6 0 3 14 1 546.6 0 1 15 1 546.6 0 1 17 4 546.6 0 4 18 2 546.6 0 2 19 1 546.6 0 1 20 3 546.6 0 3 21 5 546.6 0 5 22 9 546.6 0 9 23 19 546.6 0 19 24 5 546.6 0 5 25 36 546.6 0 36 26 6 546.6 0 6 27 11 546.6 0 11 28 5 546.6 0 5 29 2 546.6 0 2 30 9 546.6 0 9 31 2 546.6 0 2 32 8 546.6 0 8 33 8 546.6 0 8 34 171 546.6 0 171 35 185 546.6 0 185 36 7 546.6 0 7 37 4 546.6 0 4 38 2 546.6 0 2 39 2 546.6 0 2 40 2 546.6 0 2 41 4 546.6 0 4 42 7 546.6 0 7 43 1 546.6 0 1 44 3 546.6 0 3 45 2 546.6 0 2 46 1 546.6 0 1 47 5 546.6 0 5 48 4 546.6 0 4 49 7 546.6 0 7 50 5 546.6 0 5 51 3 546.6 0 3 52 4 546.6 0 4 53 3 546.6 0 3 54 2 546.6 0 2 55 2 546.6 0 2 56 5 546.6 0 5 57 3 546.6 0 3 58 7 546.6 0 7 59 9 546.6 0 9 60 6 546.6 0 6 61 2 546.6 0 2 62 4 546.6 0 4 63 2 546.6 0 2 64 3 546.6 0 3 66 4 546.6 0 4 68 4 546.6 0 4 69 3 546.6 0 3 70 3 546.6 0 3 71 5 546.6 0 5 72 3 546.6 0 3 73 5 546.6 0 5 74 24 546.6 0 24 75 18 546.6 0 18 76 17 546.6 0 17 77 21 546.6 0 21 78 19 546.6 0 19 79 20 546.6 0 20 80 12 546.6 0 12 81 23 546.6 0 23 82 26 546.6 0 26 83 48 546.6 0 48 84 11 546.6 0 11 85 9 546.6 0 9 86 21 546.6 0 21 87 29 546.6 0 29 88 28 546.6 0 28 89 35 546.6 0 35 90 25 546.6 0 25 91 26 546.6 0 26 92 14 546.6 0 14 93 39 546.6 0 39 94 33 546.6 0 33 95 36 546.6 0 36 96 50 546.6 0 50 97 82 546.6 0 82 98 84 546.6 0 84 99 445 546.6 0 445 100 539 546.6 0 539 101 588 546.6 0 588 102 48 546.6 0 48 103 52 546.6 0 52 104 21 546.6 0 21 105 27 546.6 0 27 106 38 546.6 0 38 107 24 546.6 0 24 108 46 546.6 0 46 109 79 546.6 0 79 110 80 546.6 0 80 111 740 546.6 0 740 112 1190 546.6 0 1190 113 1957 546.6 0 1957 114 1173 546.6 0 1173 115 950 546.6 0 950 116 16 546.6 0 16 117 19 546.6 0 19 118 46 546.6 0 46 119 24 546.6 0 24 120 17 546.6 0 17 121 12 546.6 0 12 122 15 546.6 0 15 123 15 546.6 0 15 124 18 546.6 0 18 125 13 546.6 0 13 126 28 546.6 0 28 127 32 546.6 0 32 128 50 546.6 0 50 129 181 546.6 0 181 130 211 546.6 0 211 131 499 546.6 0 499 132 46 546.6 0 46 133 57 546.6 0 57 134 47 546.6 0 47 135 82 546.6 0 82 136 329 546.6 0 329 137 205 546.6 0 205 138 55 546.6 0 55 139 63 546.6 0 63 140 50 546.6 0 50 141 62 546.6 0 62 142 629 546.6 0 629 143 941 546.6 0 941 144 1540 546.6 0 1540 145 1239 546.6 0 1239 146 128 546.6 0 128 147 88 546.6 0 88 148 28 546.6 0 28 149 30 546.6 0 30 150 20 546.6 0 20 151 15 546.6 0 15 152 32 546.6 0 32 153 18 546.6 0 18 154 20 546.6 0 20 155 18 546.6 0 18 156 37 546.6 0 37 157 31 546.6 0 31 158 49 546.6 0 49 159 37 546.6 0 37 160 36 546.6 0 36 161 39 546.6 0 39 162 39 546.6 0 39 163 19 546.6 0 19 164 15 546.6 0 15 165 19 546.6 0 19 166 19 546.6 0 19 167 24 546.6 0 24 168 30 546.6 0 30 169 13 546.6 0 13 170 13 546.6 0 13 171 9 546.6 0 9 172 15 546.6 0 15 173 13 546.6 0 13 174 11 546.6 0 11 175 23 546.6 0 23 176 32 546.6 0 32 177 28 546.6 0 28 178 31 546.6 0 31 179 22 546.6 0 22 180 13 546.6 0 13 181 12 546.6 0 12 182 15 546.6 0 15 183 21 546.6 0 21 184 24 546.6 0 24 185 17 546.6 0 17 186 33 546.6 0 33 187 10 546.6 0 10 188 51 546.6 0 51 189 51 546.6 0 51 190 165 546.6 0 165 191 20 546.6 0 20 192 20 546.6 0 20 193 25 546.6 0 25 194 12 546.6 0 12 195 25 546.6 0 25 196 17 546.6 0 17 197 20 546.6 0 20 198 17 546.6 0 17 199 16 546.6 0 16 200 22 546.6 0 22 201 26 546.6 0 26 202 48 546.6 0 48 203 38 546.6 0 38 204 37 546.6 0 37 205 17 546.6 0 17 206 35 546.6 0 35 207 21 546.6 0 21 208 28 546.6 0 28 209 29 546.6 0 29 210 33 546.6 0 33 211 26 546.6 0 26 212 30 546.6 0 30 213 17 546.6 0 17 214 33 546.6 0 33 215 18 546.6 0 18 216 26 546.6 0 26 217 28 546.6 0 28 218 27 546.6 0 27 219 36 546.6 0 36 220 20 546.6 0 20 221 39 546.6 0 39 222 47 546.6 0 47 223 61 546.6 0 61 224 164 546.6 0 164 225 63 546.6 0 63 226 203 546.6 0 203 227 24 546.6 0 24 228 19 546.6 0 19 229 24 546.6 0 24 230 30 546.6 0 30 231 32 546.6 0 32 232 35 546.6 0 35 233 41 546.6 0 41 234 62 546.6 0 62 235 60 546.6 0 60 236 39 546.6 0 39 237 53 546.6 0 53 238 39 546.6 0 39 239 44 546.6 0 44 240 73 546.6 0 73 241 93 546.6 0 93 242 47 546.6 0 47 243 98 546.6 0 98 244 56 546.6 0 56 245 42 546.6 0 42 246 22 546.6 0 22 247 40 546.6 0 40 248 33 546.6 0 33 249 32 546.6 0 32 250 38 546.6 0 38 251 35 546.6 0 35 252 54 546.6 0 54 253 37 546.6 0 37 254 38 546.6 0 38 255 71 546.6 0 71 256 64 546.6 0 64 257 146 546.6 0 146 258 97 546.6 0 97 259 133 546.6 0 133 260 72 546.6 0 72 261 72 546.6 0 72 262 165 546.6 0 165 263 149 546.6 0 149 264 302 546.6 0 302 265 105 546.6 0 105 266 26 546.6 0 26 267 28 546.6 0 28 268 42 546.6 0 42 269 38 546.6 0 38 270 70 546.6 0 70 271 63 546.6 0 63 272 60 546.6 0 60 273 62 546.6 0 62 274 32 546.6 0 32 275 121 546.6 0 121 276 296 546.6 0 296 277 221 546.6 0 221 278 121 546.6 0 121 279 66 546.6 0 66 280 45 546.6 0 45 281 50 546.6 0 50 282 192 546.6 0 192 283 189 546.6 0 189 284 325 546.6 0 325 285 89 546.6 0 89 286 36 546.6 0 36 287 40 546.6 0 40 288 41 546.6 0 41 289 53 546.6 0 53 290 67 546.6 0 67 291 119 546.6 0 119 292 193 546.6 0 193 293 85 546.6 0 85 294 167 546.6 0 167 295 170 546.6 0 170 296 425 546.6 0 425 297 74 546.6 0 74 298 65 546.6 0 65 299 79 546.6 0 79 300 59 546.6 0 59 301 85 546.6 0 85 Finished in 5.48 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 110,559 Reads with adapters: 41,893 (37.9%) Reads that were too short: 44 (0.0%) Reads written (passing filters): 41,849 (37.9%) Total basepairs processed: 30,363,801 bp Total written (filtered): 11,435,477 bp (37.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41893 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 2 0.0 3 0 0 0 1 1 18 21 0.0 3 0 0 15 5 1 19 409 0.0 3 0 324 54 22 9 20 40931 0.0 4 38590 1877 343 86 35 21 94 0.0 4 15 75 3 0 1 22 15 0.0 4 0 0 7 2 6 23 76 0.0 4 0 0 0 55 21 24 2 0.0 4 0 0 0 1 1 28 3 0.0 4 1 0 0 1 1 35 10 0.0 4 0 0 0 0 10 38 1 0.0 4 0 0 0 1 51 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 101 3 0.0 4 0 0 0 0 3 114 1 0.0 4 0 0 0 0 1 122 6 0.0 4 0 0 0 1 5 123 2 0.0 4 0 0 0 0 2 125 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 0 5 127 262 0.0 4 0 0 0 0 262 128 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 10 0.0 4 0 0 0 0 10 158 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 249 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 268 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.44 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 41,849 Reads with adapters: 2,391 (5.7%) Reads written (passing filters): 41,849 (100.0%) Total basepairs processed: 11,435,477 bp Total written (filtered): 11,381,427 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2391 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.0% C: 12.4% G: 71.2% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 204 653.9 0 204 4 23 163.5 0 23 5 3 40.9 0 3 9 15 0.2 0 4 11 10 24 0.0 1 0 24 11 1460 0.0 1 79 1381 12 490 0.0 1 16 474 13 13 0.0 1 1 12 15 9 0.0 1 0 9 16 3 0.0 1 0 3 152 4 0.0 1 3 1 169 1 0.0 1 1 191 3 0.0 1 0 3 193 1 0.0 1 0 1 210 123 0.0 1 92 31 211 6 0.0 1 6 225 9 0.0 1 9