This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.02 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 98,427 (4.4%) Reads that were too short: 11,747 (0.5%) Reads written (passing filters): 86,680 (3.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 23,321,974 bp (3.5%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 98427 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2385 546.6 0 2385 7 35840 546.6 0 35840 8 937 546.6 0 937 9 29484 546.6 0 29484 10 75 546.6 0 75 11 13 546.6 0 13 12 35 546.6 0 35 13 37 546.6 0 37 14 19 546.6 0 19 15 28 546.6 0 28 16 27 546.6 0 27 17 31 546.6 0 31 18 29 546.6 0 29 19 30 546.6 0 30 20 19 546.6 0 19 21 18 546.6 0 18 22 24 546.6 0 24 23 19 546.6 0 19 24 19 546.6 0 19 25 20 546.6 0 20 26 21 546.6 0 21 27 34 546.6 0 34 28 32 546.6 0 32 29 42 546.6 0 42 30 46 546.6 0 46 31 28 546.6 0 28 32 24 546.6 0 24 33 42 546.6 0 42 34 26 546.6 0 26 35 30 546.6 0 30 36 36 546.6 0 36 37 26 546.6 0 26 38 43 546.6 0 43 39 43 546.6 0 43 40 39 546.6 0 39 41 34 546.6 0 34 42 33 546.6 0 33 43 43 546.6 0 43 44 118 546.6 0 118 45 62 546.6 0 62 46 101 546.6 0 101 47 20 546.6 0 20 48 28 546.6 0 28 49 30 546.6 0 30 50 20 546.6 0 20 51 45 546.6 0 45 52 49 546.6 0 49 53 49 546.6 0 49 54 21 546.6 0 21 55 23 546.6 0 23 56 46 546.6 0 46 57 67 546.6 0 67 58 218 546.6 0 218 59 185 546.6 0 185 60 310 546.6 0 310 61 32 546.6 0 32 62 20 546.6 0 20 63 16 546.6 0 16 64 27 546.6 0 27 65 23 546.6 0 23 66 35 546.6 0 35 67 37 546.6 0 37 68 36 546.6 0 36 69 20 546.6 0 20 70 30 546.6 0 30 71 27 546.6 0 27 72 25 546.6 0 25 73 52 546.6 0 52 74 40 546.6 0 40 75 41 546.6 0 41 76 23 546.6 0 23 77 33 546.6 0 33 78 29 546.6 0 29 79 15 546.6 0 15 80 26 546.6 0 26 81 39 546.6 0 39 82 25 546.6 0 25 83 51 546.6 0 51 84 31 546.6 0 31 85 40 546.6 0 40 86 35 546.6 0 35 87 28 546.6 0 28 88 24 546.6 0 24 89 41 546.6 0 41 90 38 546.6 0 38 91 48 546.6 0 48 92 56 546.6 0 56 93 107 546.6 0 107 94 54 546.6 0 54 95 39 546.6 0 39 96 36 546.6 0 36 97 60 546.6 0 60 98 55 546.6 0 55 99 49 546.6 0 49 100 23 546.6 0 23 101 48 546.6 0 48 102 28 546.6 0 28 103 43 546.6 0 43 104 45 546.6 0 45 105 65 546.6 0 65 106 609 546.6 0 609 107 217 546.6 0 217 108 387 546.6 0 387 109 60 546.6 0 60 110 52 546.6 0 52 111 44 546.6 0 44 112 41 546.6 0 41 113 43 546.6 0 43 114 242 546.6 0 242 115 536 546.6 0 536 116 635 546.6 0 635 117 29 546.6 0 29 118 31 546.6 0 31 119 58 546.6 0 58 120 26 546.6 0 26 121 44 546.6 0 44 122 30 546.6 0 30 123 51 546.6 0 51 124 49 546.6 0 49 125 73 546.6 0 73 126 42 546.6 0 42 127 57 546.6 0 57 128 141 546.6 0 141 129 327 546.6 0 327 130 299 546.6 0 299 131 64 546.6 0 64 132 67 546.6 0 67 133 60 546.6 0 60 134 191 546.6 0 191 135 133 546.6 0 133 136 164 546.6 0 164 137 340 546.6 0 340 138 358 546.6 0 358 139 298 546.6 0 298 140 269 546.6 0 269 141 365 546.6 0 365 142 360 546.6 0 360 143 180 546.6 0 180 144 62 546.6 0 62 145 52 546.6 0 52 146 49 546.6 0 49 147 62 546.6 0 62 148 546 546.6 0 546 149 649 546.6 0 649 150 1778 546.6 0 1778 151 40 546.6 0 40 152 34 546.6 0 34 153 45 546.6 0 45 154 62 546.6 0 62 155 328 546.6 0 328 156 105 546.6 0 105 157 244 546.6 0 244 158 59 546.6 0 59 159 32 546.6 0 32 160 28 546.6 0 28 161 70 546.6 0 70 162 106 546.6 0 106 163 571 546.6 0 571 164 164 546.6 0 164 165 267 546.6 0 267 166 49 546.6 0 49 167 34 546.6 0 34 168 40 546.6 0 40 169 31 546.6 0 31 170 25 546.6 0 25 171 22 546.6 0 22 172 32 546.6 0 32 173 25 546.6 0 25 174 22 546.6 0 22 175 31 546.6 0 31 176 25 546.6 0 25 177 24 546.6 0 24 178 29 546.6 0 29 179 50 546.6 0 50 180 134 546.6 0 134 181 102 546.6 0 102 182 63 546.6 0 63 183 56 546.6 0 56 184 57 546.6 0 57 185 51 546.6 0 51 186 48 546.6 0 48 187 28 546.6 0 28 188 24 546.6 0 24 189 49 546.6 0 49 190 43 546.6 0 43 191 53 546.6 0 53 192 56 546.6 0 56 193 48 546.6 0 48 194 31 546.6 0 31 195 30 546.6 0 30 196 37 546.6 0 37 197 32 546.6 0 32 198 61 546.6 0 61 199 44 546.6 0 44 200 29 546.6 0 29 201 42 546.6 0 42 202 113 546.6 0 113 203 96 546.6 0 96 204 102 546.6 0 102 205 33 546.6 0 33 206 32 546.6 0 32 207 39 546.6 0 39 208 38 546.6 0 38 209 48 546.6 0 48 210 50 546.6 0 50 211 34 546.6 0 34 212 35 546.6 0 35 213 54 546.6 0 54 214 78 546.6 0 78 215 85 546.6 0 85 216 76 546.6 0 76 217 67 546.6 0 67 218 46 546.6 0 46 219 27 546.6 0 27 220 31 546.6 0 31 221 34 546.6 0 34 222 48 546.6 0 48 223 34 546.6 0 34 224 48 546.6 0 48 225 31 546.6 0 31 226 41 546.6 0 41 227 49 546.6 0 49 228 46 546.6 0 46 229 43 546.6 0 43 230 38 546.6 0 38 231 56 546.6 0 56 232 76 546.6 0 76 233 110 546.6 0 110 234 146 546.6 0 146 235 105 546.6 0 105 236 58 546.6 0 58 237 36 546.6 0 36 238 42 546.6 0 42 239 33 546.6 0 33 240 51 546.6 0 51 241 38 546.6 0 38 242 57 546.6 0 57 243 61 546.6 0 61 244 64 546.6 0 64 245 48 546.6 0 48 246 34 546.6 0 34 247 58 546.6 0 58 248 84 546.6 0 84 249 40 546.6 0 40 250 34 546.6 0 34 251 68 546.6 0 68 252 69 546.6 0 69 253 94 546.6 0 94 254 81 546.6 0 81 255 134 546.6 0 134 256 60 546.6 0 60 257 79 546.6 0 79 258 56 546.6 0 56 259 50 546.6 0 50 260 70 546.6 0 70 261 92 546.6 0 92 262 112 546.6 0 112 263 233 546.6 0 233 264 66 546.6 0 66 265 59 546.6 0 59 266 79 546.6 0 79 267 96 546.6 0 96 268 45 546.6 0 45 269 107 546.6 0 107 270 124 546.6 0 124 271 632 546.6 0 632 272 300 546.6 0 300 273 469 546.6 0 469 274 65 546.6 0 65 275 58 546.6 0 58 276 59 546.6 0 59 277 56 546.6 0 56 278 118 546.6 0 118 279 169 546.6 0 169 280 144 546.6 0 144 281 64 546.6 0 64 282 61 546.6 0 61 283 93 546.6 0 93 284 100 546.6 0 100 285 85 546.6 0 85 286 55 546.6 0 55 287 58 546.6 0 58 288 59 546.6 0 59 289 68 546.6 0 68 290 104 546.6 0 104 291 100 546.6 0 100 292 191 546.6 0 191 293 151 546.6 0 151 294 401 546.6 0 401 295 1358 546.6 0 1358 296 1069 546.6 0 1069 297 874 546.6 0 874 298 149 546.6 0 149 299 44 546.6 0 44 300 59 546.6 0 59 301 33 546.6 0 33 Finished in 4.41 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 86,680 Reads with adapters: 31,242 (36.0%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 31,211 (36.0%) Total basepairs processed: 23,321,974 bp Total written (filtered): 8,442,204 bp (36.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31242 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 29 0.0 3 0 0 18 7 4 19 352 0.0 3 0 305 19 17 11 20 30109 0.0 4 28169 1483 346 77 34 21 75 0.0 4 11 61 2 1 22 11 0.0 4 0 0 7 1 3 23 166 0.0 4 0 0 0 126 40 27 1 0.0 4 0 0 0 1 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 48 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 1 70 1 0.0 4 1 80 1 0.0 4 1 91 2 0.0 4 2 93 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 443 0.0 4 0 0 0 0 443 128 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 141 2 0.0 4 0 0 0 0 2 153 5 0.0 4 0 0 0 0 5 158 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 217 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.13 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 31,211 Reads with adapters: 8,230 (26.4%) Reads written (passing filters): 31,211 (100.0%) Total basepairs processed: 8,442,204 bp Total written (filtered): 8,304,922 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8230 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.8% C: 7.9% G: 85.4% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 487.7 0 17 4 3 121.9 0 3 6 4 7.6 0 4 7 2 1.9 0 2 8 5 0.5 0 5 9 2351 0.1 0 266 2085 10 5181 0.0 1 402 4779 11 379 0.0 1 15 364 12 25 0.0 1 0 25 13 20 0.0 1 1 19 150 6 0.0 1 2 4 208 42 0.0 1 36 6 209 2 0.0 1 1 1 210 3 0.0 1 2 1 211 1 0.0 1 1 215 1 0.0 1 1 256 1 0.0 1 1 257 173 0.0 1 161 12 258 11 0.0 1 11 259 2 0.0 1 2 260 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.