This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 59.68 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 89,485 (4.0%) Reads that were too short: 5,464 (0.2%) Reads written (passing filters): 84,021 (3.8%) Total basepairs processed: 673,916,425 bp Total written (filtered): 22,988,448 bp (3.4%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 89485 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1783 546.6 0 1783 7 31508 546.6 0 31508 8 946 546.6 0 946 9 36048 546.6 0 36048 10 46 546.6 0 46 11 15 546.6 0 15 12 24 546.6 0 24 13 12 546.6 0 12 14 8 546.6 0 8 15 15 546.6 0 15 16 18 546.6 0 18 17 20 546.6 0 20 18 22 546.6 0 22 19 13 546.6 0 13 20 10 546.6 0 10 21 15 546.6 0 15 22 18 546.6 0 18 23 10 546.6 0 10 24 18 546.6 0 18 25 8 546.6 0 8 26 12 546.6 0 12 27 18 546.6 0 18 28 18 546.6 0 18 29 13 546.6 0 13 30 20 546.6 0 20 31 26 546.6 0 26 32 17 546.6 0 17 33 26 546.6 0 26 34 14 546.6 0 14 35 14 546.6 0 14 36 24 546.6 0 24 37 10 546.6 0 10 38 25 546.6 0 25 39 14 546.6 0 14 40 27 546.6 0 27 41 16 546.6 0 16 42 17 546.6 0 17 43 34 546.6 0 34 44 131 546.6 0 131 45 53 546.6 0 53 46 64 546.6 0 64 47 17 546.6 0 17 48 23 546.6 0 23 49 19 546.6 0 19 50 17 546.6 0 17 51 21 546.6 0 21 52 24 546.6 0 24 53 16 546.6 0 16 54 27 546.6 0 27 55 16 546.6 0 16 56 27 546.6 0 27 57 20 546.6 0 20 58 50 546.6 0 50 59 43 546.6 0 43 60 33 546.6 0 33 61 19 546.6 0 19 62 12 546.6 0 12 63 19 546.6 0 19 64 19 546.6 0 19 65 28 546.6 0 28 66 17 546.6 0 17 67 17 546.6 0 17 68 16 546.6 0 16 69 21 546.6 0 21 70 14 546.6 0 14 71 13 546.6 0 13 72 18 546.6 0 18 73 24 546.6 0 24 74 19 546.6 0 19 75 22 546.6 0 22 76 21 546.6 0 21 77 20 546.6 0 20 78 14 546.6 0 14 79 8 546.6 0 8 80 28 546.6 0 28 81 23 546.6 0 23 82 22 546.6 0 22 83 31 546.6 0 31 84 19 546.6 0 19 85 21 546.6 0 21 86 26 546.6 0 26 87 19 546.6 0 19 88 24 546.6 0 24 89 32 546.6 0 32 90 27 546.6 0 27 91 39 546.6 0 39 92 35 546.6 0 35 93 47 546.6 0 47 94 39 546.6 0 39 95 27 546.6 0 27 96 26 546.6 0 26 97 35 546.6 0 35 98 36 546.6 0 36 99 24 546.6 0 24 100 26 546.6 0 26 101 26 546.6 0 26 102 29 546.6 0 29 103 32 546.6 0 32 104 40 546.6 0 40 105 64 546.6 0 64 106 612 546.6 0 612 107 188 546.6 0 188 108 385 546.6 0 385 109 48 546.6 0 48 110 25 546.6 0 25 111 41 546.6 0 41 112 33 546.6 0 33 113 37 546.6 0 37 114 252 546.6 0 252 115 601 546.6 0 601 116 753 546.6 0 753 117 21 546.6 0 21 118 15 546.6 0 15 119 29 546.6 0 29 120 32 546.6 0 32 121 39 546.6 0 39 122 28 546.6 0 28 123 28 546.6 0 28 124 25 546.6 0 25 125 28 546.6 0 28 126 25 546.6 0 25 127 46 546.6 0 46 128 68 546.6 0 68 129 134 546.6 0 134 130 115 546.6 0 115 131 35 546.6 0 35 132 61 546.6 0 61 133 49 546.6 0 49 134 162 546.6 0 162 135 139 546.6 0 139 136 133 546.6 0 133 137 316 546.6 0 316 138 363 546.6 0 363 139 216 546.6 0 216 140 229 546.6 0 229 141 278 546.6 0 278 142 221 546.6 0 221 143 99 546.6 0 99 144 41 546.6 0 41 145 38 546.6 0 38 146 43 546.6 0 43 147 37 546.6 0 37 148 589 546.6 0 589 149 612 546.6 0 612 150 1940 546.6 0 1940 151 36 546.6 0 36 152 18 546.6 0 18 153 31 546.6 0 31 154 25 546.6 0 25 155 149 546.6 0 149 156 52 546.6 0 52 157 175 546.6 0 175 158 55 546.6 0 55 159 16 546.6 0 16 160 22 546.6 0 22 161 35 546.6 0 35 162 67 546.6 0 67 163 261 546.6 0 261 164 82 546.6 0 82 165 163 546.6 0 163 166 26 546.6 0 26 167 16 546.6 0 16 168 20 546.6 0 20 169 23 546.6 0 23 170 20 546.6 0 20 171 11 546.6 0 11 172 11 546.6 0 11 173 20 546.6 0 20 174 20 546.6 0 20 175 21 546.6 0 21 176 23 546.6 0 23 177 27 546.6 0 27 178 22 546.6 0 22 179 29 546.6 0 29 180 64 546.6 0 64 181 80 546.6 0 80 182 39 546.6 0 39 183 31 546.6 0 31 184 27 546.6 0 27 185 25 546.6 0 25 186 27 546.6 0 27 187 17 546.6 0 17 188 17 546.6 0 17 189 45 546.6 0 45 190 23 546.6 0 23 191 24 546.6 0 24 192 33 546.6 0 33 193 31 546.6 0 31 194 14 546.6 0 14 195 15 546.6 0 15 196 19 546.6 0 19 197 23 546.6 0 23 198 63 546.6 0 63 199 26 546.6 0 26 200 23 546.6 0 23 201 29 546.6 0 29 202 70 546.6 0 70 203 71 546.6 0 71 204 60 546.6 0 60 205 23 546.6 0 23 206 9 546.6 0 9 207 18 546.6 0 18 208 21 546.6 0 21 209 49 546.6 0 49 210 25 546.6 0 25 211 21 546.6 0 21 212 20 546.6 0 20 213 23 546.6 0 23 214 43 546.6 0 43 215 37 546.6 0 37 216 37 546.6 0 37 217 33 546.6 0 33 218 32 546.6 0 32 219 20 546.6 0 20 220 27 546.6 0 27 221 32 546.6 0 32 222 32 546.6 0 32 223 27 546.6 0 27 224 39 546.6 0 39 225 25 546.6 0 25 226 17 546.6 0 17 227 18 546.6 0 18 228 26 546.6 0 26 229 28 546.6 0 28 230 20 546.6 0 20 231 28 546.6 0 28 232 37 546.6 0 37 233 39 546.6 0 39 234 57 546.6 0 57 235 58 546.6 0 58 236 31 546.6 0 31 237 40 546.6 0 40 238 39 546.6 0 39 239 25 546.6 0 25 240 39 546.6 0 39 241 29 546.6 0 29 242 29 546.6 0 29 243 29 546.6 0 29 244 43 546.6 0 43 245 32 546.6 0 32 246 34 546.6 0 34 247 34 546.6 0 34 248 98 546.6 0 98 249 40 546.6 0 40 250 32 546.6 0 32 251 39 546.6 0 39 252 35 546.6 0 35 253 64 546.6 0 64 254 49 546.6 0 49 255 48 546.6 0 48 256 36 546.6 0 36 257 32 546.6 0 32 258 28 546.6 0 28 259 33 546.6 0 33 260 41 546.6 0 41 261 41 546.6 0 41 262 54 546.6 0 54 263 101 546.6 0 101 264 39 546.6 0 39 265 27 546.6 0 27 266 33 546.6 0 33 267 59 546.6 0 59 268 39 546.6 0 39 269 48 546.6 0 48 270 56 546.6 0 56 271 174 546.6 0 174 272 119 546.6 0 119 273 182 546.6 0 182 274 51 546.6 0 51 275 74 546.6 0 74 276 53 546.6 0 53 277 43 546.6 0 43 278 59 546.6 0 59 279 71 546.6 0 71 280 68 546.6 0 68 281 38 546.6 0 38 282 32 546.6 0 32 283 55 546.6 0 55 284 39 546.6 0 39 285 57 546.6 0 57 286 29 546.6 0 29 287 37 546.6 0 37 288 43 546.6 0 43 289 46 546.6 0 46 290 57 546.6 0 57 291 58 546.6 0 58 292 89 546.6 0 89 293 71 546.6 0 71 294 138 546.6 0 138 295 415 546.6 0 415 296 340 546.6 0 340 297 249 546.6 0 249 298 62 546.6 0 62 299 42 546.6 0 42 300 45 546.6 0 45 301 30 546.6 0 30 Finished in 3.82 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 84,021 Reads with adapters: 32,761 (39.0%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 32,737 (39.0%) Total basepairs processed: 22,988,448 bp Total written (filtered): 9,055,546 bp (39.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32761 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 215 0.0 3 0 208 7 17 31071 0.0 3 30480 580 11 18 84 0.0 3 5 76 1 2 19 25 0.0 3 2 14 9 20 1274 0.0 3 1206 19 1 48 21 2 0.0 3 0 2 23 1 0.0 3 0 0 0 1 30 7 0.0 3 0 0 0 7 43 30 0.0 3 0 0 0 30 46 5 0.0 3 0 0 0 5 63 1 0.0 3 0 0 0 1 69 9 0.0 3 0 0 0 9 70 7 0.0 3 0 0 0 7 113 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 217 10 0.0 3 0 0 0 10 225 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.15 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 32,737 Reads with adapters: 909 (2.8%) Reads written (passing filters): 32,737 (100.0%) Total basepairs processed: 9,055,546 bp Total written (filtered): 9,052,794 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 909 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 80.0% C: 7.9% G: 5.0% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 885 511.5 0 885 4 23 127.9 0 23 5 1 32.0 0 1