This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 59.90 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 320,485 (14.3%) Reads that were too short: 31,861 (1.4%) Reads written (passing filters): 288,624 (12.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 82,821,181 bp (12.3%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 320485 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6698 546.6 0 6698 7 116726 546.6 0 116726 8 7132 546.6 0 7132 9 145011 546.6 0 145011 10 118 546.6 0 118 11 33 546.6 0 33 12 26 546.6 0 26 13 18 546.6 0 18 14 29 546.6 0 29 15 16 546.6 0 16 16 124 546.6 0 124 17 65 546.6 0 65 18 65 546.6 0 65 19 13 546.6 0 13 20 16 546.6 0 16 21 17 546.6 0 17 22 13 546.6 0 13 23 4 546.6 0 4 24 25 546.6 0 25 25 27 546.6 0 27 26 17 546.6 0 17 27 16 546.6 0 16 28 18 546.6 0 18 29 10 546.6 0 10 30 18 546.6 0 18 31 21 546.6 0 21 32 17 546.6 0 17 33 17 546.6 0 17 34 21 546.6 0 21 35 23 546.6 0 23 36 18 546.6 0 18 37 17 546.6 0 17 38 20 546.6 0 20 39 15 546.6 0 15 40 17 546.6 0 17 41 10 546.6 0 10 42 9 546.6 0 9 43 16 546.6 0 16 44 21 546.6 0 21 45 14 546.6 0 14 46 13 546.6 0 13 47 22 546.6 0 22 48 29 546.6 0 29 49 23 546.6 0 23 50 96 546.6 0 96 51 85 546.6 0 85 52 12 546.6 0 12 53 27 546.6 0 27 54 20 546.6 0 20 55 21 546.6 0 21 56 21 546.6 0 21 57 26 546.6 0 26 58 10 546.6 0 10 59 37 546.6 0 37 60 27 546.6 0 27 61 97 546.6 0 97 62 25 546.6 0 25 63 24 546.6 0 24 64 19 546.6 0 19 65 20 546.6 0 20 66 24 546.6 0 24 67 281 546.6 0 281 68 160 546.6 0 160 69 323 546.6 0 323 70 13 546.6 0 13 71 12 546.6 0 12 72 40 546.6 0 40 73 38 546.6 0 38 74 33 546.6 0 33 75 23 546.6 0 23 76 18 546.6 0 18 77 21 546.6 0 21 78 16 546.6 0 16 79 20 546.6 0 20 80 22 546.6 0 22 81 28 546.6 0 28 82 12 546.6 0 12 83 19 546.6 0 19 84 21 546.6 0 21 85 56 546.6 0 56 86 107 546.6 0 107 87 53 546.6 0 53 88 21 546.6 0 21 89 21 546.6 0 21 90 40 546.6 0 40 91 18 546.6 0 18 92 67 546.6 0 67 93 25 546.6 0 25 94 92 546.6 0 92 95 23 546.6 0 23 96 27 546.6 0 27 97 20 546.6 0 20 98 18 546.6 0 18 99 10 546.6 0 10 100 25 546.6 0 25 101 9 546.6 0 9 102 31 546.6 0 31 103 10 546.6 0 10 104 17 546.6 0 17 105 14 546.6 0 14 106 11 546.6 0 11 107 25 546.6 0 25 108 26 546.6 0 26 109 17 546.6 0 17 110 12 546.6 0 12 111 25 546.6 0 25 112 17 546.6 0 17 113 23 546.6 0 23 114 17 546.6 0 17 115 15 546.6 0 15 116 24 546.6 0 24 117 14 546.6 0 14 118 49 546.6 0 49 119 56 546.6 0 56 120 77 546.6 0 77 121 40 546.6 0 40 122 36 546.6 0 36 123 44 546.6 0 44 124 20 546.6 0 20 125 24 546.6 0 24 126 37 546.6 0 37 127 44 546.6 0 44 128 50 546.6 0 50 129 75 546.6 0 75 130 105 546.6 0 105 131 128 546.6 0 128 132 90 546.6 0 90 133 118 546.6 0 118 134 114 546.6 0 114 135 26 546.6 0 26 136 32 546.6 0 32 137 12 546.6 0 12 138 24 546.6 0 24 139 21 546.6 0 21 140 34 546.6 0 34 141 26 546.6 0 26 142 21 546.6 0 21 143 23 546.6 0 23 144 23 546.6 0 23 145 44 546.6 0 44 146 39 546.6 0 39 147 67 546.6 0 67 148 47 546.6 0 47 149 55 546.6 0 55 150 46 546.6 0 46 151 36 546.6 0 36 152 148 546.6 0 148 153 279 546.6 0 279 154 326 546.6 0 326 155 118 546.6 0 118 156 25 546.6 0 25 157 34 546.6 0 34 158 147 546.6 0 147 159 88 546.6 0 88 160 139 546.6 0 139 161 52 546.6 0 52 162 42 546.6 0 42 163 139 546.6 0 139 164 160 546.6 0 160 165 340 546.6 0 340 166 157 546.6 0 157 167 110 546.6 0 110 168 60 546.6 0 60 169 57 546.6 0 57 170 28 546.6 0 28 171 26 546.6 0 26 172 45 546.6 0 45 173 65 546.6 0 65 174 78 546.6 0 78 175 90 546.6 0 90 176 67 546.6 0 67 177 86 546.6 0 86 178 88 546.6 0 88 179 94 546.6 0 94 180 148 546.6 0 148 181 108 546.6 0 108 182 94 546.6 0 94 183 358 546.6 0 358 184 338 546.6 0 338 185 727 546.6 0 727 186 408 546.6 0 408 187 94 546.6 0 94 188 54 546.6 0 54 189 50 546.6 0 50 190 49 546.6 0 49 191 61 546.6 0 61 192 245 546.6 0 245 193 338 546.6 0 338 194 443 546.6 0 443 195 178 546.6 0 178 196 51 546.6 0 51 197 39 546.6 0 39 198 158 546.6 0 158 199 200 546.6 0 200 200 342 546.6 0 342 201 151 546.6 0 151 202 144 546.6 0 144 203 94 546.6 0 94 204 119 546.6 0 119 205 91 546.6 0 91 206 113 546.6 0 113 207 147 546.6 0 147 208 106 546.6 0 106 209 91 546.6 0 91 210 84 546.6 0 84 211 78 546.6 0 78 212 66 546.6 0 66 213 65 546.6 0 65 214 100 546.6 0 100 215 132 546.6 0 132 216 170 546.6 0 170 217 374 546.6 0 374 218 718 546.6 0 718 219 8546 546.6 0 8546 220 2477 546.6 0 2477 221 6808 546.6 0 6808 222 174 546.6 0 174 223 71 546.6 0 71 224 85 546.6 0 85 225 46 546.6 0 46 226 36 546.6 0 36 227 31 546.6 0 31 228 38 546.6 0 38 229 47 546.6 0 47 230 78 546.6 0 78 231 59 546.6 0 59 232 61 546.6 0 61 233 61 546.6 0 61 234 75 546.6 0 75 235 95 546.6 0 95 236 69 546.6 0 69 237 93 546.6 0 93 238 105 546.6 0 105 239 100 546.6 0 100 240 137 546.6 0 137 241 67 546.6 0 67 242 51 546.6 0 51 243 43 546.6 0 43 244 54 546.6 0 54 245 46 546.6 0 46 246 203 546.6 0 203 247 79 546.6 0 79 248 245 546.6 0 245 249 323 546.6 0 323 250 616 546.6 0 616 251 704 546.6 0 704 252 193 546.6 0 193 253 210 546.6 0 210 254 210 546.6 0 210 255 98 546.6 0 98 256 103 546.6 0 103 257 81 546.6 0 81 258 76 546.6 0 76 259 44 546.6 0 44 260 60 546.6 0 60 261 48 546.6 0 48 262 62 546.6 0 62 263 71 546.6 0 71 264 44 546.6 0 44 265 55 546.6 0 55 266 73 546.6 0 73 267 153 546.6 0 153 268 105 546.6 0 105 269 78 546.6 0 78 270 71 546.6 0 71 271 72 546.6 0 72 272 155 546.6 0 155 273 152 546.6 0 152 274 245 546.6 0 245 275 92 546.6 0 92 276 49 546.6 0 49 277 86 546.6 0 86 278 184 546.6 0 184 279 709 546.6 0 709 280 830 546.6 0 830 281 584 546.6 0 584 282 188 546.6 0 188 283 108 546.6 0 108 284 128 546.6 0 128 285 82 546.6 0 82 286 89 546.6 0 89 287 102 546.6 0 102 288 139 546.6 0 139 289 106 546.6 0 106 290 79 546.6 0 79 291 100 546.6 0 100 292 172 546.6 0 172 293 167 546.6 0 167 294 191 546.6 0 191 295 120 546.6 0 120 296 104 546.6 0 104 297 60 546.6 0 60 298 87 546.6 0 87 299 119 546.6 0 119 300 152 546.6 0 152 301 160 546.6 0 160 Finished in 13.86 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 288,624 Reads with adapters: 122,901 (42.6%) Reads that were too short: 108 (0.0%) Reads written (passing filters): 122,793 (42.5%) Total basepairs processed: 82,821,181 bp Total written (filtered): 33,518,724 bp (40.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 122901 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 5 0.0 3 0 0 0 1 4 18 46 0.0 3 0 0 35 8 3 19 1657 0.0 3 0 1439 141 64 13 20 119425 0.0 4 113519 4878 803 181 44 21 229 0.0 4 33 175 14 4 3 22 23 0.0 4 1 2 11 1 8 23 189 0.0 4 0 0 1 144 44 24 3 0.0 4 0 0 0 0 3 27 1 0.0 4 1 28 1 0.0 4 0 0 0 1 29 1 0.0 4 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 42 1 0.0 4 0 0 0 1 57 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 1 103 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 5 0.0 4 0 0 0 0 5 126 9 0.0 4 0 0 0 0 9 127 1158 0.0 4 0 0 0 0 1158 128 5 0.0 4 0 0 0 0 5 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 18 0.0 4 0 0 0 0 18 157 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 0 2 209 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 7 0.0 4 0 0 0 0 7 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 23 0.0 4 0 0 0 0 23 229 6 0.0 4 0 0 0 0 6 230 2 0.0 4 0 0 0 0 2 231 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 6 0.0 4 0 0 0 0 6 237 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 3 0.0 4 0 0 0 0 3 260 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 1 0 2 265 2 0.0 4 0 0 0 0 2 266 1 0.0 4 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 285 2 0.0 4 0 0 0 0 2 286 2 0.0 4 0 0 0 0 2 288 2 0.0 4 0 0 0 0 2 291 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.28 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 122,793 Reads with adapters: 14,118 (11.5%) Reads written (passing filters): 122,793 (100.0%) Total basepairs processed: 33,518,724 bp Total written (filtered): 33,304,014 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14118 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.6% C: 10.3% G: 81.8% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 409 1918.6 0 409 4 30 479.7 0 30 5 4 119.9 0 4 8 2 1.9 0 2 9 40 0.5 0 4 36 10 133 0.1 1 2 131 11 11630 0.0 1 532 11098 12 1496 0.0 1 35 1461 13 40 0.0 1 0 40 14 11 0.0 1 0 11 15 11 0.0 1 0 11 16 1 0.0 1 0 1 62 1 0.0 1 0 1 94 1 0.0 1 1 120 1 0.0 1 1 123 1 0.0 1 0 1 124 1 0.0 1 0 1 149 2 0.0 1 2 151 1 0.0 1 1 152 24 0.0 1 20 4 153 1 0.0 1 0 1 162 2 0.0 1 2 179 2 0.0 1 0 2 191 2 0.0 1 0 2 208 1 0.0 1 1 210 206 0.0 1 171 35 211 22 0.0 1 19 3 225 5 0.0 1 5 228 2 0.0 1 2 231 1 0.0 1 1 259 3 0.0 1 3 261 31 0.0 1 28 3 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.