This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.55 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 482,851 (18.2%) Reads that were too short: 30,247 (1.1%) Reads written (passing filters): 452,604 (17.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 104,199,123 bp (13.0%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 482851 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 840 649.0 0 840 7 2103 649.0 0 2103 8 37165 649.0 0 37165 9 37951 649.0 0 37951 10 52898 649.0 0 52898 11 67 649.0 0 67 12 28 649.0 0 28 13 17 649.0 0 17 14 11 649.0 0 11 15 27 649.0 0 27 16 34 649.0 0 34 17 40 649.0 0 40 18 19 649.0 0 19 19 32 649.0 0 32 20 21 649.0 0 21 21 23 649.0 0 23 22 29 649.0 0 29 23 61 649.0 0 61 24 71 649.0 0 71 25 41 649.0 0 41 26 71 649.0 0 71 27 57 649.0 0 57 28 30 649.0 0 30 29 30 649.0 0 30 30 33 649.0 0 33 31 46 649.0 0 46 32 60 649.0 0 60 33 27 649.0 0 27 34 20 649.0 0 20 35 23 649.0 0 23 36 24 649.0 0 24 37 12 649.0 0 12 38 37 649.0 0 37 39 86 649.0 0 86 40 104 649.0 0 104 41 170 649.0 0 170 42 58 649.0 0 58 43 23 649.0 0 23 44 28 649.0 0 28 45 22 649.0 0 22 46 17 649.0 0 17 47 34 649.0 0 34 48 63 649.0 0 63 49 62 649.0 0 62 50 49 649.0 0 49 51 87 649.0 0 87 52 162 649.0 0 162 53 154 649.0 0 154 54 112 649.0 0 112 55 40 649.0 0 40 56 38 649.0 0 38 57 94 649.0 0 94 58 637 649.0 0 637 59 52 649.0 0 52 60 63 649.0 0 63 61 39 649.0 0 39 62 60 649.0 0 60 63 76 649.0 0 76 64 42 649.0 0 42 65 52 649.0 0 52 66 93 649.0 0 93 67 67 649.0 0 67 68 153 649.0 0 153 69 64 649.0 0 64 70 73 649.0 0 73 71 110 649.0 0 110 72 167 649.0 0 167 73 96 649.0 0 96 74 121 649.0 0 121 75 208 649.0 0 208 76 290 649.0 0 290 77 356 649.0 0 356 78 759 649.0 0 759 79 1635 649.0 0 1635 80 432 649.0 0 432 81 588 649.0 0 588 82 1235 649.0 0 1235 83 582 649.0 0 582 84 570 649.0 0 570 85 1084 649.0 0 1084 86 1503 649.0 0 1503 87 1762 649.0 0 1762 88 1189 649.0 0 1189 89 2236 649.0 0 2236 90 2988 649.0 0 2988 91 5368 649.0 0 5368 92 99703 649.0 0 99703 93 52236 649.0 0 52236 94 127710 649.0 0 127710 95 910 649.0 0 910 96 52 649.0 0 52 97 53 649.0 0 53 98 50 649.0 0 50 99 37 649.0 0 37 100 24 649.0 0 24 101 13 649.0 0 13 102 26 649.0 0 26 103 19 649.0 0 19 104 19 649.0 0 19 105 22 649.0 0 22 106 18 649.0 0 18 107 22 649.0 0 22 108 32 649.0 0 32 109 23 649.0 0 23 110 48 649.0 0 48 111 36 649.0 0 36 112 91 649.0 0 91 113 29 649.0 0 29 114 59 649.0 0 59 115 57 649.0 0 57 116 66 649.0 0 66 117 98 649.0 0 98 118 35 649.0 0 35 119 17 649.0 0 17 120 14 649.0 0 14 121 10 649.0 0 10 122 9 649.0 0 9 123 17 649.0 0 17 124 12 649.0 0 12 125 25 649.0 0 25 126 21 649.0 0 21 127 50 649.0 0 50 128 33 649.0 0 33 129 57 649.0 0 57 130 47 649.0 0 47 131 44 649.0 0 44 132 27 649.0 0 27 133 29 649.0 0 29 134 29 649.0 0 29 135 22 649.0 0 22 136 25 649.0 0 25 137 38 649.0 0 38 138 30 649.0 0 30 139 31 649.0 0 31 140 19 649.0 0 19 141 18 649.0 0 18 142 29 649.0 0 29 143 48 649.0 0 48 144 42 649.0 0 42 145 66 649.0 0 66 146 17 649.0 0 17 147 43 649.0 0 43 148 26 649.0 0 26 149 19 649.0 0 19 150 14 649.0 0 14 151 29 649.0 0 29 152 12 649.0 0 12 153 17 649.0 0 17 154 22 649.0 0 22 155 17 649.0 0 17 156 25 649.0 0 25 157 33 649.0 0 33 158 73 649.0 0 73 159 28 649.0 0 28 160 30 649.0 0 30 161 24 649.0 0 24 162 15 649.0 0 15 163 19 649.0 0 19 164 23 649.0 0 23 165 26 649.0 0 26 166 32 649.0 0 32 167 15 649.0 0 15 168 19 649.0 0 19 169 17 649.0 0 17 170 21 649.0 0 21 171 26 649.0 0 26 172 36 649.0 0 36 173 31 649.0 0 31 174 46 649.0 0 46 175 39 649.0 0 39 176 71 649.0 0 71 177 228 649.0 0 228 178 1035 649.0 0 1035 179 1567 649.0 0 1567 180 591 649.0 0 591 181 33 649.0 0 33 182 23 649.0 0 23 183 36 649.0 0 36 184 35 649.0 0 35 185 35 649.0 0 35 186 52 649.0 0 52 187 72 649.0 0 72 188 165 649.0 0 165 189 68 649.0 0 68 190 117 649.0 0 117 191 133 649.0 0 133 192 90 649.0 0 90 193 46 649.0 0 46 194 96 649.0 0 96 195 119 649.0 0 119 196 150 649.0 0 150 197 161 649.0 0 161 198 183 649.0 0 183 199 234 649.0 0 234 200 399 649.0 0 399 201 5673 649.0 0 5673 202 4173 649.0 0 4173 203 7890 649.0 0 7890 204 223 649.0 0 223 205 495 649.0 0 495 206 777 649.0 0 777 207 891 649.0 0 891 208 303 649.0 0 303 209 226 649.0 0 226 210 134 649.0 0 134 211 61 649.0 0 61 212 72 649.0 0 72 213 55 649.0 0 55 214 51 649.0 0 51 215 107 649.0 0 107 216 106 649.0 0 106 217 107 649.0 0 107 218 79 649.0 0 79 219 43 649.0 0 43 220 52 649.0 0 52 221 207 649.0 0 207 222 361 649.0 0 361 223 551 649.0 0 551 224 253 649.0 0 253 225 485 649.0 0 485 226 689 649.0 0 689 227 867 649.0 0 867 228 657 649.0 0 657 229 513 649.0 0 513 230 19 649.0 0 19 231 28 649.0 0 28 232 33 649.0 0 33 233 27 649.0 0 27 234 17 649.0 0 17 235 30 649.0 0 30 236 82 649.0 0 82 237 69 649.0 0 69 238 70 649.0 0 70 239 23 649.0 0 23 240 15 649.0 0 15 241 16 649.0 0 16 242 17 649.0 0 17 243 37 649.0 0 37 244 36 649.0 0 36 245 36 649.0 0 36 246 84 649.0 0 84 247 54 649.0 0 54 248 24 649.0 0 24 249 32 649.0 0 32 250 26 649.0 0 26 251 32 649.0 0 32 252 17 649.0 0 17 253 28 649.0 0 28 254 24 649.0 0 24 255 29 649.0 0 29 256 33 649.0 0 33 257 56 649.0 0 56 258 143 649.0 0 143 259 614 649.0 0 614 260 820 649.0 0 820 261 608 649.0 0 608 262 252 649.0 0 252 263 242 649.0 0 242 264 151 649.0 0 151 265 64 649.0 0 64 266 36 649.0 0 36 267 55 649.0 0 55 268 35 649.0 0 35 269 170 649.0 0 170 270 252 649.0 0 252 271 229 649.0 0 229 272 251 649.0 0 251 273 69 649.0 0 69 274 75 649.0 0 75 275 36 649.0 0 36 276 63 649.0 0 63 277 97 649.0 0 97 278 84 649.0 0 84 279 80 649.0 0 80 280 147 649.0 0 147 281 148 649.0 0 148 282 141 649.0 0 141 283 100 649.0 0 100 284 127 649.0 0 127 285 588 649.0 0 588 286 402 649.0 0 402 287 308 649.0 0 308 288 224 649.0 0 224 289 36 649.0 0 36 290 41 649.0 0 41 291 34 649.0 0 34 292 28 649.0 0 28 293 55 649.0 0 55 294 50 649.0 0 50 295 58 649.0 0 58 296 127 649.0 0 127 297 922 649.0 0 922 298 411 649.0 0 411 299 380 649.0 0 380 300 38 649.0 0 38 301 34 649.0 0 34 Finished in 19.60 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 452,604 Reads with adapters: 34,159 (7.5%) Reads that were too short: 135 (0.0%) Reads written (passing filters): 34,024 (7.5%) Total basepairs processed: 104,199,123 bp Total written (filtered): 9,126,298 bp (8.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 34159 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 32 0.0 3 0 0 21 11 19 626 0.0 3 0 573 38 8 7 20 31964 0.0 4 30459 1256 187 47 15 21 71 0.0 4 15 50 3 3 22 11 0.0 4 1 0 7 1 2 23 88 0.0 4 0 0 0 75 13 24 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 1 32 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 1 43 2 0.0 4 0 0 0 0 2 45 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 1 63 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 3 0.0 4 0 0 0 1 2 68 42 0.0 4 0 0 0 0 42 94 4 0.0 4 0 0 0 0 4 96 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 1 122 6 0.0 4 0 0 0 0 6 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 14 0.0 4 0 0 0 0 14 127 1136 0.0 4 0 0 0 0 1136 128 5 0.0 4 0 0 0 0 5 129 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 136 6 0.0 4 0 0 0 0 6 137 8 0.0 4 0 0 0 1 7 138 3 0.0 4 0 0 0 0 3 139 2 0.0 4 1 0 0 0 1 140 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 148 8 0.0 4 0 0 0 0 8 149 16 0.0 4 0 0 0 1 15 150 8 0.0 4 0 0 0 0 8 151 5 0.0 4 0 0 0 0 5 153 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 1 166 3 0.0 4 0 0 0 0 3 168 4 0.0 4 0 0 0 0 4 169 4 0.0 4 0 0 0 0 4 171 2 0.0 4 0 0 0 0 2 172 4 0.0 4 0 0 0 0 4 173 3 0.0 4 0 0 0 0 3 174 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 1 0 0 1 197 1 0.0 4 0 0 0 0 1 198 3 0.0 4 0 0 0 0 3 199 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 5 0.0 4 0 0 0 0 5 229 6 0.0 4 0 0 0 0 6 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 1 1 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.24 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 34,024 Reads with adapters: 7,269 (21.4%) Reads written (passing filters): 34,024 (100.0%) Total basepairs processed: 9,126,298 bp Total written (filtered): 9,050,133 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7269 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 8.0% G: 88.1% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 531.6 0 14 4 5 132.9 0 5 7 1 2.1 0 1 8 4 0.5 0 4 9 990 0.1 0 91 899 10 4037 0.0 1 266 3771 11 2169 0.0 1 83 2086 12 21 0.0 1 2 19 13 11 0.0 1 0 11 14 1 0.0 1 0 1 150 14 0.0 1 10 4 208 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.