This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.69 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 438,093 (16.5%) Reads that were too short: 25,924 (1.0%) Reads written (passing filters): 412,169 (15.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 96,747,722 bp (12.1%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 438093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 590 649.0 0 590 7 2119 649.0 0 2119 8 36295 649.0 0 36295 9 42516 649.0 0 42516 10 61179 649.0 0 61179 11 50 649.0 0 50 12 13 649.0 0 13 13 16 649.0 0 16 14 14 649.0 0 14 15 15 649.0 0 15 16 21 649.0 0 21 17 26 649.0 0 26 18 13 649.0 0 13 19 35 649.0 0 35 20 22 649.0 0 22 21 30 649.0 0 30 22 31 649.0 0 31 23 44 649.0 0 44 24 59 649.0 0 59 25 33 649.0 0 33 26 56 649.0 0 56 27 66 649.0 0 66 28 30 649.0 0 30 29 34 649.0 0 34 30 31 649.0 0 31 31 37 649.0 0 37 32 54 649.0 0 54 33 32 649.0 0 32 34 19 649.0 0 19 35 35 649.0 0 35 36 35 649.0 0 35 37 16 649.0 0 16 38 30 649.0 0 30 39 59 649.0 0 59 40 50 649.0 0 50 41 157 649.0 0 157 42 34 649.0 0 34 43 27 649.0 0 27 44 12 649.0 0 12 45 21 649.0 0 21 46 24 649.0 0 24 47 32 649.0 0 32 48 103 649.0 0 103 49 61 649.0 0 61 50 58 649.0 0 58 51 32 649.0 0 32 52 63 649.0 0 63 53 105 649.0 0 105 54 109 649.0 0 109 55 41 649.0 0 41 56 36 649.0 0 36 57 75 649.0 0 75 58 610 649.0 0 610 59 34 649.0 0 34 60 60 649.0 0 60 61 64 649.0 0 64 62 73 649.0 0 73 63 68 649.0 0 68 64 25 649.0 0 25 65 48 649.0 0 48 66 75 649.0 0 75 67 52 649.0 0 52 68 152 649.0 0 152 69 83 649.0 0 83 70 59 649.0 0 59 71 108 649.0 0 108 72 151 649.0 0 151 73 97 649.0 0 97 74 87 649.0 0 87 75 199 649.0 0 199 76 270 649.0 0 270 77 266 649.0 0 266 78 683 649.0 0 683 79 1516 649.0 0 1516 80 345 649.0 0 345 81 488 649.0 0 488 82 1040 649.0 0 1040 83 463 649.0 0 463 84 436 649.0 0 436 85 1024 649.0 0 1024 86 1307 649.0 0 1307 87 1378 649.0 0 1378 88 926 649.0 0 926 89 1676 649.0 0 1676 90 2043 649.0 0 2043 91 3750 649.0 0 3750 92 79128 649.0 0 79128 93 42783 649.0 0 42783 94 111753 649.0 0 111753 95 775 649.0 0 775 96 28 649.0 0 28 97 42 649.0 0 42 98 28 649.0 0 28 99 28 649.0 0 28 100 14 649.0 0 14 101 24 649.0 0 24 102 28 649.0 0 28 103 16 649.0 0 16 104 16 649.0 0 16 105 13 649.0 0 13 106 15 649.0 0 15 107 19 649.0 0 19 108 22 649.0 0 22 109 37 649.0 0 37 110 29 649.0 0 29 111 31 649.0 0 31 112 64 649.0 0 64 113 19 649.0 0 19 114 24 649.0 0 24 115 28 649.0 0 28 116 45 649.0 0 45 117 80 649.0 0 80 118 39 649.0 0 39 119 19 649.0 0 19 120 13 649.0 0 13 121 13 649.0 0 13 122 10 649.0 0 10 123 13 649.0 0 13 124 20 649.0 0 20 125 25 649.0 0 25 126 16 649.0 0 16 127 35 649.0 0 35 128 21 649.0 0 21 129 23 649.0 0 23 130 26 649.0 0 26 131 18 649.0 0 18 132 24 649.0 0 24 133 16 649.0 0 16 134 17 649.0 0 17 135 19 649.0 0 19 136 22 649.0 0 22 137 41 649.0 0 41 138 30 649.0 0 30 139 12 649.0 0 12 140 14 649.0 0 14 141 19 649.0 0 19 142 20 649.0 0 20 143 23 649.0 0 23 144 18 649.0 0 18 145 29 649.0 0 29 146 18 649.0 0 18 147 31 649.0 0 31 148 29 649.0 0 29 149 17 649.0 0 17 150 21 649.0 0 21 151 26 649.0 0 26 152 16 649.0 0 16 153 20 649.0 0 20 154 25 649.0 0 25 155 11 649.0 0 11 156 22 649.0 0 22 157 28 649.0 0 28 158 31 649.0 0 31 159 23 649.0 0 23 160 17 649.0 0 17 161 27 649.0 0 27 162 16 649.0 0 16 163 24 649.0 0 24 164 18 649.0 0 18 165 19 649.0 0 19 166 19 649.0 0 19 167 25 649.0 0 25 168 24 649.0 0 24 169 19 649.0 0 19 170 15 649.0 0 15 171 37 649.0 0 37 172 28 649.0 0 28 173 30 649.0 0 30 174 51 649.0 0 51 175 42 649.0 0 42 176 78 649.0 0 78 177 241 649.0 0 241 178 1316 649.0 0 1316 179 1834 649.0 0 1834 180 812 649.0 0 812 181 24 649.0 0 24 182 22 649.0 0 22 183 34 649.0 0 34 184 33 649.0 0 33 185 35 649.0 0 35 186 47 649.0 0 47 187 80 649.0 0 80 188 137 649.0 0 137 189 63 649.0 0 63 190 127 649.0 0 127 191 160 649.0 0 160 192 80 649.0 0 80 193 54 649.0 0 54 194 112 649.0 0 112 195 145 649.0 0 145 196 123 649.0 0 123 197 118 649.0 0 118 198 145 649.0 0 145 199 202 649.0 0 202 200 283 649.0 0 283 201 5100 649.0 0 5100 202 3895 649.0 0 3895 203 7702 649.0 0 7702 204 208 649.0 0 208 205 492 649.0 0 492 206 740 649.0 0 740 207 852 649.0 0 852 208 227 649.0 0 227 209 174 649.0 0 174 210 128 649.0 0 128 211 75 649.0 0 75 212 71 649.0 0 71 213 35 649.0 0 35 214 41 649.0 0 41 215 64 649.0 0 64 216 64 649.0 0 64 217 78 649.0 0 78 218 79 649.0 0 79 219 26 649.0 0 26 220 48 649.0 0 48 221 224 649.0 0 224 222 224 649.0 0 224 223 364 649.0 0 364 224 148 649.0 0 148 225 266 649.0 0 266 226 448 649.0 0 448 227 662 649.0 0 662 228 539 649.0 0 539 229 569 649.0 0 569 230 20 649.0 0 20 231 10 649.0 0 10 232 21 649.0 0 21 233 27 649.0 0 27 234 17 649.0 0 17 235 34 649.0 0 34 236 58 649.0 0 58 237 55 649.0 0 55 238 45 649.0 0 45 239 22 649.0 0 22 240 10 649.0 0 10 241 27 649.0 0 27 242 18 649.0 0 18 243 31 649.0 0 31 244 24 649.0 0 24 245 33 649.0 0 33 246 35 649.0 0 35 247 37 649.0 0 37 248 31 649.0 0 31 249 42 649.0 0 42 250 23 649.0 0 23 251 29 649.0 0 29 252 19 649.0 0 19 253 26 649.0 0 26 254 18 649.0 0 18 255 23 649.0 0 23 256 29 649.0 0 29 257 48 649.0 0 48 258 98 649.0 0 98 259 605 649.0 0 605 260 727 649.0 0 727 261 719 649.0 0 719 262 334 649.0 0 334 263 321 649.0 0 321 264 164 649.0 0 164 265 26 649.0 0 26 266 28 649.0 0 28 267 27 649.0 0 27 268 19 649.0 0 19 269 61 649.0 0 61 270 95 649.0 0 95 271 122 649.0 0 122 272 91 649.0 0 91 273 44 649.0 0 44 274 39 649.0 0 39 275 18 649.0 0 18 276 74 649.0 0 74 277 91 649.0 0 91 278 63 649.0 0 63 279 56 649.0 0 56 280 105 649.0 0 105 281 96 649.0 0 96 282 82 649.0 0 82 283 65 649.0 0 65 284 100 649.0 0 100 285 420 649.0 0 420 286 256 649.0 0 256 287 206 649.0 0 206 288 156 649.0 0 156 289 24 649.0 0 24 290 23 649.0 0 23 291 39 649.0 0 39 292 25 649.0 0 25 293 46 649.0 0 46 294 32 649.0 0 32 295 33 649.0 0 33 296 92 649.0 0 92 297 580 649.0 0 580 298 202 649.0 0 202 299 216 649.0 0 216 300 35 649.0 0 35 301 14 649.0 0 14 Finished in 16.17 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 412,169 Reads with adapters: 36,113 (8.8%) Reads that were too short: 155 (0.0%) Reads written (passing filters): 35,958 (8.7%) Total basepairs processed: 96,747,722 bp Total written (filtered): 9,608,896 bp (9.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36113 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 163 0.0 3 0 161 2 17 33734 0.0 3 33017 694 22 1 18 88 0.0 3 7 76 4 1 19 22 0.0 3 1 0 18 3 20 22 0.0 3 2 0 0 20 28 1 0.0 3 0 0 0 1 30 3 0.0 3 0 0 0 3 32 15 0.0 3 0 0 0 15 34 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 51 2 0.0 3 0 0 0 2 52 5 0.0 3 0 0 0 5 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 74 50 0.0 3 0 0 0 50 75 3 0.0 3 0 0 0 3 76 5 0.0 3 0 0 0 5 77 19 0.0 3 0 0 0 19 78 1 0.0 3 0 0 0 1 79 13 0.0 3 0 0 0 13 80 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 98 11 0.0 3 0 0 0 11 99 1216 0.0 3 0 0 5 1211 100 356 0.0 3 0 0 2 354 102 128 0.0 3 0 0 2 126 103 60 0.0 3 0 1 1 58 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 144 5 0.0 3 4 0 0 1 147 1 0.0 3 0 0 0 1 150 4 0.0 3 0 0 0 4 151 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 158 3 0.0 3 1 0 0 2 159 7 0.0 3 0 0 0 7 160 9 0.0 3 0 0 0 9 161 1 0.0 3 0 0 0 1 162 3 0.0 3 0 0 0 3 164 3 0.0 3 0 0 0 3 172 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 189 7 0.0 3 0 0 1 6 191 8 0.0 3 0 0 1 7 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 200 42 0.0 3 0 0 0 42 201 19 0.0 3 0 0 0 19 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 10 0.0 3 0 0 1 9 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 239 3 0.0 3 0 0 1 2 275 1 0.0 3 0 0 0 1 276 3 0.0 3 0 0 0 3 278 4 0.0 3 0 0 0 4 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.24 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 35,958 Reads with adapters: 230 (0.6%) Reads written (passing filters): 35,958 (100.0%) Total basepairs processed: 9,608,896 bp Total written (filtered): 9,608,154 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 230 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.3% C: 4.8% G: 28.3% T: 5.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 181 561.8 0 181 4 46 140.5 0 46 5 3 35.1 0 3