This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.57 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 367,328 (13.8%) Reads that were too short: 20,298 (0.8%) Reads written (passing filters): 347,030 (13.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 88,855,553 bp (11.1%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 367328 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2440 649.0 0 2440 7 96153 649.0 0 96153 8 85014 649.0 0 85014 9 86 649.0 0 86 10 268 649.0 0 268 11 44 649.0 0 44 12 26 649.0 0 26 13 40 649.0 0 40 14 30 649.0 0 30 15 3 649.0 0 3 16 5 649.0 0 5 17 7 649.0 0 7 18 6 649.0 0 6 19 15 649.0 0 15 20 15 649.0 0 15 21 25 649.0 0 25 22 24 649.0 0 24 23 22 649.0 0 22 24 16 649.0 0 16 25 21 649.0 0 21 26 15 649.0 0 15 27 15 649.0 0 15 28 49 649.0 0 49 29 102 649.0 0 102 30 28 649.0 0 28 31 16 649.0 0 16 32 16 649.0 0 16 33 64 649.0 0 64 34 64 649.0 0 64 35 48 649.0 0 48 36 57 649.0 0 57 37 28 649.0 0 28 38 15 649.0 0 15 39 16 649.0 0 16 40 18 649.0 0 18 41 15 649.0 0 15 42 50 649.0 0 50 43 44 649.0 0 44 44 52 649.0 0 52 45 37 649.0 0 37 46 62 649.0 0 62 47 43 649.0 0 43 48 35 649.0 0 35 49 58 649.0 0 58 50 48 649.0 0 48 51 69 649.0 0 69 52 224 649.0 0 224 53 81 649.0 0 81 54 73 649.0 0 73 55 103 649.0 0 103 56 32 649.0 0 32 57 15 649.0 0 15 58 12 649.0 0 12 59 49 649.0 0 49 60 178 649.0 0 178 61 127 649.0 0 127 62 355 649.0 0 355 63 173 649.0 0 173 64 232 649.0 0 232 65 887 649.0 0 887 66 676 649.0 0 676 67 1556 649.0 0 1556 68 142 649.0 0 142 69 113 649.0 0 113 70 76 649.0 0 76 71 125 649.0 0 125 72 449 649.0 0 449 73 350 649.0 0 350 74 671 649.0 0 671 75 987 649.0 0 987 76 1034 649.0 0 1034 77 2395 649.0 0 2395 78 3993 649.0 0 3993 79 53528 649.0 0 53528 80 26893 649.0 0 26893 81 44669 649.0 0 44669 82 514 649.0 0 514 83 373 649.0 0 373 84 103 649.0 0 103 85 90 649.0 0 90 86 117 649.0 0 117 87 141 649.0 0 141 88 79 649.0 0 79 89 64 649.0 0 64 90 128 649.0 0 128 91 32 649.0 0 32 92 87 649.0 0 87 93 72 649.0 0 72 94 50 649.0 0 50 95 94 649.0 0 94 96 65 649.0 0 65 97 30 649.0 0 30 98 31 649.0 0 31 99 47 649.0 0 47 100 33 649.0 0 33 101 50 649.0 0 50 102 57 649.0 0 57 103 51 649.0 0 51 104 40 649.0 0 40 105 33 649.0 0 33 106 33 649.0 0 33 107 22 649.0 0 22 108 35 649.0 0 35 109 34 649.0 0 34 110 45 649.0 0 45 111 68 649.0 0 68 112 67 649.0 0 67 113 88 649.0 0 88 114 95 649.0 0 95 115 121 649.0 0 121 116 409 649.0 0 409 117 315 649.0 0 315 118 779 649.0 0 779 119 223 649.0 0 223 120 200 649.0 0 200 121 180 649.0 0 180 122 146 649.0 0 146 123 227 649.0 0 227 124 168 649.0 0 168 125 243 649.0 0 243 126 264 649.0 0 264 127 243 649.0 0 243 128 117 649.0 0 117 129 156 649.0 0 156 130 260 649.0 0 260 131 283 649.0 0 283 132 372 649.0 0 372 133 160 649.0 0 160 134 132 649.0 0 132 135 344 649.0 0 344 136 107 649.0 0 107 137 261 649.0 0 261 138 128 649.0 0 128 139 159 649.0 0 159 140 386 649.0 0 386 141 240 649.0 0 240 142 330 649.0 0 330 143 152 649.0 0 152 144 272 649.0 0 272 145 223 649.0 0 223 146 198 649.0 0 198 147 231 649.0 0 231 148 183 649.0 0 183 149 212 649.0 0 212 150 852 649.0 0 852 151 579 649.0 0 579 152 1972 649.0 0 1972 153 219 649.0 0 219 154 122 649.0 0 122 155 107 649.0 0 107 156 99 649.0 0 99 157 115 649.0 0 115 158 129 649.0 0 129 159 146 649.0 0 146 160 102 649.0 0 102 161 95 649.0 0 95 162 99 649.0 0 99 163 111 649.0 0 111 164 75 649.0 0 75 165 114 649.0 0 114 166 115 649.0 0 115 167 154 649.0 0 154 168 135 649.0 0 135 169 150 649.0 0 150 170 184 649.0 0 184 171 166 649.0 0 166 172 234 649.0 0 234 173 378 649.0 0 378 174 244 649.0 0 244 175 102 649.0 0 102 176 91 649.0 0 91 177 109 649.0 0 109 178 108 649.0 0 108 179 120 649.0 0 120 180 110 649.0 0 110 181 111 649.0 0 111 182 129 649.0 0 129 183 106 649.0 0 106 184 120 649.0 0 120 185 115 649.0 0 115 186 217 649.0 0 217 187 181 649.0 0 181 188 138 649.0 0 138 189 116 649.0 0 116 190 102 649.0 0 102 191 127 649.0 0 127 192 122 649.0 0 122 193 142 649.0 0 142 194 123 649.0 0 123 195 132 649.0 0 132 196 103 649.0 0 103 197 148 649.0 0 148 198 156 649.0 0 156 199 257 649.0 0 257 200 131 649.0 0 131 201 104 649.0 0 104 202 119 649.0 0 119 203 98 649.0 0 98 204 119 649.0 0 119 205 209 649.0 0 209 206 129 649.0 0 129 207 90 649.0 0 90 208 109 649.0 0 109 209 104 649.0 0 104 210 158 649.0 0 158 211 105 649.0 0 105 212 135 649.0 0 135 213 125 649.0 0 125 214 125 649.0 0 125 215 145 649.0 0 145 216 171 649.0 0 171 217 669 649.0 0 669 218 542 649.0 0 542 219 453 649.0 0 453 220 147 649.0 0 147 221 166 649.0 0 166 222 203 649.0 0 203 223 227 649.0 0 227 224 207 649.0 0 207 225 180 649.0 0 180 226 705 649.0 0 705 227 624 649.0 0 624 228 1847 649.0 0 1847 229 201 649.0 0 201 230 140 649.0 0 140 231 99 649.0 0 99 232 117 649.0 0 117 233 96 649.0 0 96 234 82 649.0 0 82 235 95 649.0 0 95 236 104 649.0 0 104 237 94 649.0 0 94 238 125 649.0 0 125 239 133 649.0 0 133 240 111 649.0 0 111 241 145 649.0 0 145 242 122 649.0 0 122 243 144 649.0 0 144 244 164 649.0 0 164 245 137 649.0 0 137 246 225 649.0 0 225 247 203 649.0 0 203 248 172 649.0 0 172 249 182 649.0 0 182 250 178 649.0 0 178 251 164 649.0 0 164 252 174 649.0 0 174 253 229 649.0 0 229 254 125 649.0 0 125 255 138 649.0 0 138 256 142 649.0 0 142 257 141 649.0 0 141 258 101 649.0 0 101 259 102 649.0 0 102 260 112 649.0 0 112 261 120 649.0 0 120 262 157 649.0 0 157 263 169 649.0 0 169 264 162 649.0 0 162 265 149 649.0 0 149 266 214 649.0 0 214 267 142 649.0 0 142 268 160 649.0 0 160 269 121 649.0 0 121 270 132 649.0 0 132 271 186 649.0 0 186 272 183 649.0 0 183 273 168 649.0 0 168 274 241 649.0 0 241 275 239 649.0 0 239 276 216 649.0 0 216 277 185 649.0 0 185 278 180 649.0 0 180 279 286 649.0 0 286 280 224 649.0 0 224 281 270 649.0 0 270 282 187 649.0 0 187 283 152 649.0 0 152 284 129 649.0 0 129 285 127 649.0 0 127 286 184 649.0 0 184 287 191 649.0 0 191 288 186 649.0 0 186 289 192 649.0 0 192 290 242 649.0 0 242 291 240 649.0 0 240 292 261 649.0 0 261 293 209 649.0 0 209 294 203 649.0 0 203 295 159 649.0 0 159 296 166 649.0 0 166 297 181 649.0 0 181 298 246 649.0 0 246 299 219 649.0 0 219 300 333 649.0 0 333 301 179 649.0 0 179 Finished in 15.99 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 347,030 Reads with adapters: 90,541 (26.1%) Reads that were too short: 136 (0.0%) Reads written (passing filters): 90,405 (26.1%) Total basepairs processed: 88,855,553 bp Total written (filtered): 24,593,689 bp (27.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 90541 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 13 0.0 3 0 0 0 7 6 18 44 0.0 3 0 0 29 9 6 19 914 0.0 3 0 747 80 59 28 20 85420 0.0 4 81151 3526 580 127 36 21 239 0.0 4 28 188 9 10 4 22 2636 0.0 4 2473 116 34 7 6 23 309 0.0 4 4 4 1 221 79 24 3 0.0 4 1 0 1 0 1 25 8 0.0 4 0 0 0 5 3 27 1 0.0 4 0 1 28 2 0.0 4 0 0 0 0 2 30 1 0.0 4 1 31 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 46 1 0.0 4 1 50 1 0.0 4 1 51 1 0.0 4 0 1 57 1 0.0 4 0 1 58 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 1 72 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 4 0.0 4 0 0 0 0 4 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 125 9 0.0 4 0 0 0 0 9 126 16 0.0 4 0 0 0 0 16 127 772 0.0 4 0 0 0 0 772 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 134 2 0.0 4 1 0 0 0 1 141 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 159 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 208 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 34 0.0 4 0 0 0 2 32 229 22 0.0 4 0 0 0 1 21 230 3 0.0 4 0 0 0 0 3 231 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 4 0.0 4 0 0 0 0 4 238 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 4 0.0 4 0 0 0 0 4 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 286 2 0.0 4 0 0 0 0 2 287 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.21 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 90,405 Reads with adapters: 12,737 (14.1%) Reads written (passing filters): 90,405 (100.0%) Total basepairs processed: 24,593,689 bp Total written (filtered): 24,449,884 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 12737 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 8.5% G: 86.2% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 196 1412.6 0 196 4 17 353.1 0 17 6 12 22.1 0 12 8 1 1.4 0 1 9 185 0.3 0 11 174 10 2464 0.1 1 190 2274 11 9493 0.0 1 304 9189 12 275 0.0 1 9 266 13 7 0.0 1 0 7 14 7 0.0 1 0 7 15 8 0.0 1 0 8 18 14 0.0 1 7 7 19 2 0.0 1 2 21 1 0.0 1 0 1 22 6 0.0 1 4 2 35 2 0.0 1 2 36 1 0.0 1 1 41 1 0.0 1 1 52 1 0.0 1 0 1 128 1 0.0 1 1 151 3 0.0 1 3 152 1 0.0 1 1 153 1 0.0 1 1 156 15 0.0 1 10 5 178 1 0.0 1 0 1 208 2 0.0 1 2 209 14 0.0 1 9 5 210 1 0.0 1 0 1 223 3 0.0 1 3 230 1 0.0 1 1 235 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.