This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.32 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 385,615 (14.5%) Reads that were too short: 15,019 (0.6%) Reads written (passing filters): 370,596 (13.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 94,691,049 bp (11.8%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 385615 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1288 649.0 0 1288 7 92193 649.0 0 92193 8 94815 649.0 0 94815 9 78 649.0 0 78 10 95 649.0 0 95 11 46 649.0 0 46 12 59 649.0 0 59 13 32 649.0 0 32 14 6 649.0 0 6 15 2 649.0 0 2 16 6 649.0 0 6 17 9 649.0 0 9 18 7 649.0 0 7 19 11 649.0 0 11 20 17 649.0 0 17 21 35 649.0 0 35 22 26 649.0 0 26 23 21 649.0 0 21 24 6 649.0 0 6 25 14 649.0 0 14 26 6 649.0 0 6 27 16 649.0 0 16 28 61 649.0 0 61 29 100 649.0 0 100 30 24 649.0 0 24 31 5 649.0 0 5 32 15 649.0 0 15 33 61 649.0 0 61 34 63 649.0 0 63 35 38 649.0 0 38 36 43 649.0 0 43 37 26 649.0 0 26 38 12 649.0 0 12 39 29 649.0 0 29 40 28 649.0 0 28 41 11 649.0 0 11 42 59 649.0 0 59 43 53 649.0 0 53 44 46 649.0 0 46 45 62 649.0 0 62 46 73 649.0 0 73 47 67 649.0 0 67 48 29 649.0 0 29 49 66 649.0 0 66 50 53 649.0 0 53 51 81 649.0 0 81 52 299 649.0 0 299 53 47 649.0 0 47 54 52 649.0 0 52 55 85 649.0 0 85 56 36 649.0 0 36 57 18 649.0 0 18 58 26 649.0 0 26 59 61 649.0 0 61 60 195 649.0 0 195 61 113 649.0 0 113 62 335 649.0 0 335 63 87 649.0 0 87 64 158 649.0 0 158 65 510 649.0 0 510 66 371 649.0 0 371 67 821 649.0 0 821 68 142 649.0 0 142 69 123 649.0 0 123 70 72 649.0 0 72 71 156 649.0 0 156 72 310 649.0 0 310 73 333 649.0 0 333 74 466 649.0 0 466 75 935 649.0 0 935 76 1089 649.0 0 1089 77 2290 649.0 0 2290 78 4022 649.0 0 4022 79 63896 649.0 0 63896 80 33914 649.0 0 33914 81 53484 649.0 0 53484 82 425 649.0 0 425 83 214 649.0 0 214 84 110 649.0 0 110 85 62 649.0 0 62 86 76 649.0 0 76 87 88 649.0 0 88 88 67 649.0 0 67 89 38 649.0 0 38 90 42 649.0 0 42 91 48 649.0 0 48 92 44 649.0 0 44 93 59 649.0 0 59 94 62 649.0 0 62 95 44 649.0 0 44 96 39 649.0 0 39 97 44 649.0 0 44 98 39 649.0 0 39 99 55 649.0 0 55 100 58 649.0 0 58 101 53 649.0 0 53 102 58 649.0 0 58 103 26 649.0 0 26 104 41 649.0 0 41 105 46 649.0 0 46 106 38 649.0 0 38 107 29 649.0 0 29 108 34 649.0 0 34 109 49 649.0 0 49 110 53 649.0 0 53 111 62 649.0 0 62 112 52 649.0 0 52 113 67 649.0 0 67 114 62 649.0 0 62 115 89 649.0 0 89 116 211 649.0 0 211 117 171 649.0 0 171 118 710 649.0 0 710 119 214 649.0 0 214 120 207 649.0 0 207 121 108 649.0 0 108 122 113 649.0 0 113 123 163 649.0 0 163 124 81 649.0 0 81 125 139 649.0 0 139 126 111 649.0 0 111 127 140 649.0 0 140 128 71 649.0 0 71 129 106 649.0 0 106 130 204 649.0 0 204 131 146 649.0 0 146 132 273 649.0 0 273 133 101 649.0 0 101 134 140 649.0 0 140 135 297 649.0 0 297 136 87 649.0 0 87 137 212 649.0 0 212 138 81 649.0 0 81 139 114 649.0 0 114 140 342 649.0 0 342 141 215 649.0 0 215 142 278 649.0 0 278 143 117 649.0 0 117 144 186 649.0 0 186 145 136 649.0 0 136 146 118 649.0 0 118 147 146 649.0 0 146 148 129 649.0 0 129 149 167 649.0 0 167 150 622 649.0 0 622 151 440 649.0 0 440 152 1652 649.0 0 1652 153 120 649.0 0 120 154 89 649.0 0 89 155 71 649.0 0 71 156 91 649.0 0 91 157 87 649.0 0 87 158 103 649.0 0 103 159 111 649.0 0 111 160 77 649.0 0 77 161 63 649.0 0 63 162 59 649.0 0 59 163 66 649.0 0 66 164 65 649.0 0 65 165 82 649.0 0 82 166 89 649.0 0 89 167 120 649.0 0 120 168 83 649.0 0 83 169 114 649.0 0 114 170 140 649.0 0 140 171 119 649.0 0 119 172 169 649.0 0 169 173 323 649.0 0 323 174 175 649.0 0 175 175 69 649.0 0 69 176 68 649.0 0 68 177 74 649.0 0 74 178 97 649.0 0 97 179 86 649.0 0 86 180 70 649.0 0 70 181 78 649.0 0 78 182 75 649.0 0 75 183 82 649.0 0 82 184 94 649.0 0 94 185 90 649.0 0 90 186 167 649.0 0 167 187 116 649.0 0 116 188 134 649.0 0 134 189 84 649.0 0 84 190 63 649.0 0 63 191 95 649.0 0 95 192 69 649.0 0 69 193 89 649.0 0 89 194 97 649.0 0 97 195 106 649.0 0 106 196 85 649.0 0 85 197 118 649.0 0 118 198 126 649.0 0 126 199 296 649.0 0 296 200 100 649.0 0 100 201 92 649.0 0 92 202 67 649.0 0 67 203 75 649.0 0 75 204 95 649.0 0 95 205 176 649.0 0 176 206 150 649.0 0 150 207 63 649.0 0 63 208 70 649.0 0 70 209 95 649.0 0 95 210 125 649.0 0 125 211 98 649.0 0 98 212 108 649.0 0 108 213 105 649.0 0 105 214 110 649.0 0 110 215 81 649.0 0 81 216 140 649.0 0 140 217 782 649.0 0 782 218 613 649.0 0 613 219 475 649.0 0 475 220 146 649.0 0 146 221 107 649.0 0 107 222 156 649.0 0 156 223 149 649.0 0 149 224 123 649.0 0 123 225 131 649.0 0 131 226 574 649.0 0 574 227 432 649.0 0 432 228 2168 649.0 0 2168 229 92 649.0 0 92 230 85 649.0 0 85 231 86 649.0 0 86 232 112 649.0 0 112 233 77 649.0 0 77 234 63 649.0 0 63 235 57 649.0 0 57 236 74 649.0 0 74 237 80 649.0 0 80 238 85 649.0 0 85 239 112 649.0 0 112 240 105 649.0 0 105 241 98 649.0 0 98 242 81 649.0 0 81 243 81 649.0 0 81 244 89 649.0 0 89 245 98 649.0 0 98 246 108 649.0 0 108 247 90 649.0 0 90 248 97 649.0 0 97 249 92 649.0 0 92 250 93 649.0 0 93 251 110 649.0 0 110 252 114 649.0 0 114 253 180 649.0 0 180 254 82 649.0 0 82 255 74 649.0 0 74 256 80 649.0 0 80 257 122 649.0 0 122 258 84 649.0 0 84 259 69 649.0 0 69 260 69 649.0 0 69 261 85 649.0 0 85 262 86 649.0 0 86 263 91 649.0 0 91 264 71 649.0 0 71 265 93 649.0 0 93 266 113 649.0 0 113 267 63 649.0 0 63 268 101 649.0 0 101 269 61 649.0 0 61 270 127 649.0 0 127 271 179 649.0 0 179 272 96 649.0 0 96 273 129 649.0 0 129 274 163 649.0 0 163 275 223 649.0 0 223 276 116 649.0 0 116 277 92 649.0 0 92 278 86 649.0 0 86 279 135 649.0 0 135 280 108 649.0 0 108 281 150 649.0 0 150 282 108 649.0 0 108 283 78 649.0 0 78 284 84 649.0 0 84 285 71 649.0 0 71 286 117 649.0 0 117 287 112 649.0 0 112 288 83 649.0 0 83 289 101 649.0 0 101 290 144 649.0 0 144 291 139 649.0 0 139 292 141 649.0 0 141 293 110 649.0 0 110 294 97 649.0 0 97 295 102 649.0 0 102 296 95 649.0 0 95 297 136 649.0 0 136 298 151 649.0 0 151 299 136 649.0 0 136 300 177 649.0 0 177 301 116 649.0 0 116 Finished in 15.44 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 370,596 Reads with adapters: 88,658 (23.9%) Reads that were too short: 110 (0.0%) Reads written (passing filters): 88,548 (23.9%) Total basepairs processed: 94,691,049 bp Total written (filtered): 24,502,263 bp (25.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 88658 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 18 0.0 3 0 0 18 16 477 0.0 3 0 463 12 2 17 87617 0.0 3 85847 1721 45 4 18 172 0.0 3 6 160 6 19 48 0.0 3 3 2 31 12 20 40 0.0 3 0 0 1 39 21 2 0.0 3 0 0 1 1 22 1 0.0 3 1 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 3 0.0 3 0 0 0 3 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 1 31 6 0.0 3 0 0 0 6 36 1 0.0 3 0 0 0 1 41 30 0.0 3 0 0 0 30 43 1 0.0 3 0 0 0 1 44 10 0.0 3 0 0 0 10 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 6 0.0 3 0 0 0 6 58 1 0.0 3 0 0 0 1 67 9 0.0 3 0 0 0 9 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 1 75 5 0.0 3 0 0 0 5 76 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 11 0.0 3 0 0 0 11 107 1 0.0 3 0 0 0 1 108 4 0.0 3 0 0 0 4 109 4 0.0 3 0 0 0 4 110 3 0.0 3 0 0 1 2 111 4 0.0 3 0 0 2 2 114 2 0.0 3 0 0 0 2 118 2 0.0 3 1 0 0 1 120 2 0.0 3 0 0 0 2 124 1 0.0 3 0 0 0 1 125 17 0.0 3 0 0 1 16 126 1 0.0 3 0 0 0 1 127 7 0.0 3 0 0 0 7 131 2 0.0 3 0 0 0 2 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 144 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 146 7 0.0 3 0 0 0 7 149 5 0.0 3 0 0 0 5 150 12 0.0 3 0 0 0 12 153 1 0.0 3 0 0 0 1 163 2 0.0 3 1 0 0 1 164 9 0.0 3 0 0 0 9 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 1 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 1 199 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 207 7 0.0 3 0 0 0 7 208 4 0.0 3 0 0 0 4 209 10 0.0 3 0 0 1 9 210 11 0.0 3 0 0 0 11 211 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 1 237 2 0.0 3 0 0 1 1 242 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 7 0.0 3 0 0 0 7 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.13 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 88,548 Reads with adapters: 504 (0.6%) Reads written (passing filters): 88,548 (100.0%) Total basepairs processed: 24,502,263 bp Total written (filtered): 24,500,716 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 504 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.2% C: 9.5% G: 49.8% T: 19.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 470 1383.6 0 470 4 33 345.9 0 33 5 1 86.5 0 1