This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.88 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 61,619 (2.3%) Reads that were too short: 7,735 (0.3%) Reads written (passing filters): 53,884 (2.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 13,271,775 bp (1.7%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 61619 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1421 649.0 0 1421 7 11862 649.0 0 11862 8 361 649.0 0 361 9 9064 649.0 0 9064 10 17 649.0 0 17 11 10 649.0 0 10 12 9 649.0 0 9 13 16 649.0 0 16 14 14 649.0 0 14 15 16 649.0 0 16 16 11 649.0 0 11 17 12 649.0 0 12 18 14 649.0 0 14 19 17 649.0 0 17 20 18 649.0 0 18 21 17 649.0 0 17 22 18 649.0 0 18 23 30 649.0 0 30 24 20 649.0 0 20 25 19 649.0 0 19 26 17 649.0 0 17 27 49 649.0 0 49 28 105 649.0 0 105 29 102 649.0 0 102 30 198 649.0 0 198 31 13 649.0 0 13 32 14 649.0 0 14 33 172 649.0 0 172 34 183 649.0 0 183 35 194 649.0 0 194 36 22 649.0 0 22 37 27 649.0 0 27 38 17 649.0 0 17 39 19 649.0 0 19 40 18 649.0 0 18 41 16 649.0 0 16 42 40 649.0 0 40 43 39 649.0 0 39 44 49 649.0 0 49 45 60 649.0 0 60 46 37 649.0 0 37 47 29 649.0 0 29 48 33 649.0 0 33 49 43 649.0 0 43 50 81 649.0 0 81 51 94 649.0 0 94 52 154 649.0 0 154 53 636 649.0 0 636 54 3386 649.0 0 3386 55 4037 649.0 0 4037 56 3760 649.0 0 3760 57 26 649.0 0 26 58 20 649.0 0 20 59 14 649.0 0 14 60 18 649.0 0 18 61 10 649.0 0 10 62 11 649.0 0 11 63 10 649.0 0 10 64 12 649.0 0 12 65 7 649.0 0 7 66 10 649.0 0 10 67 28 649.0 0 28 68 18 649.0 0 18 69 18 649.0 0 18 70 21 649.0 0 21 71 10 649.0 0 10 72 15 649.0 0 15 73 10 649.0 0 10 74 27 649.0 0 27 75 12 649.0 0 12 76 11 649.0 0 11 77 26 649.0 0 26 78 19 649.0 0 19 79 32 649.0 0 32 80 38 649.0 0 38 81 82 649.0 0 82 82 25 649.0 0 25 83 31 649.0 0 31 84 42 649.0 0 42 85 27 649.0 0 27 86 28 649.0 0 28 87 43 649.0 0 43 88 43 649.0 0 43 89 51 649.0 0 51 90 46 649.0 0 46 91 74 649.0 0 74 92 102 649.0 0 102 93 215 649.0 0 215 94 4544 649.0 0 4544 95 1277 649.0 0 1277 96 2380 649.0 0 2380 97 20 649.0 0 20 98 31 649.0 0 31 99 20 649.0 0 20 100 17 649.0 0 17 101 14 649.0 0 14 102 19 649.0 0 19 103 16 649.0 0 16 104 15 649.0 0 15 105 20 649.0 0 20 106 9 649.0 0 9 107 24 649.0 0 24 108 18 649.0 0 18 109 198 649.0 0 198 110 92 649.0 0 92 111 194 649.0 0 194 112 16 649.0 0 16 113 17 649.0 0 17 114 24 649.0 0 24 115 32 649.0 0 32 116 22 649.0 0 22 117 48 649.0 0 48 118 57 649.0 0 57 119 65 649.0 0 65 120 133 649.0 0 133 121 430 649.0 0 430 122 264 649.0 0 264 123 227 649.0 0 227 124 27 649.0 0 27 125 36 649.0 0 36 126 40 649.0 0 40 127 52 649.0 0 52 128 29 649.0 0 29 129 28 649.0 0 28 130 47 649.0 0 47 131 26 649.0 0 26 132 27 649.0 0 27 133 54 649.0 0 54 134 40 649.0 0 40 135 33 649.0 0 33 136 110 649.0 0 110 137 181 649.0 0 181 138 93 649.0 0 93 139 92 649.0 0 92 140 90 649.0 0 90 141 165 649.0 0 165 142 59 649.0 0 59 143 35 649.0 0 35 144 44 649.0 0 44 145 137 649.0 0 137 146 38 649.0 0 38 147 112 649.0 0 112 148 75 649.0 0 75 149 203 649.0 0 203 150 59 649.0 0 59 151 100 649.0 0 100 152 59 649.0 0 59 153 91 649.0 0 91 154 56 649.0 0 56 155 38 649.0 0 38 156 56 649.0 0 56 157 60 649.0 0 60 158 49 649.0 0 49 159 63 649.0 0 63 160 352 649.0 0 352 161 289 649.0 0 289 162 196 649.0 0 196 163 115 649.0 0 115 164 38 649.0 0 38 165 87 649.0 0 87 166 123 649.0 0 123 167 112 649.0 0 112 168 73 649.0 0 73 169 37 649.0 0 37 170 43 649.0 0 43 171 32 649.0 0 32 172 53 649.0 0 53 173 58 649.0 0 58 174 44 649.0 0 44 175 109 649.0 0 109 176 143 649.0 0 143 177 107 649.0 0 107 178 85 649.0 0 85 179 39 649.0 0 39 180 96 649.0 0 96 181 193 649.0 0 193 182 130 649.0 0 130 183 49 649.0 0 49 184 35 649.0 0 35 185 39 649.0 0 39 186 58 649.0 0 58 187 26 649.0 0 26 188 45 649.0 0 45 189 36 649.0 0 36 190 27 649.0 0 27 191 37 649.0 0 37 192 46 649.0 0 46 193 54 649.0 0 54 194 37 649.0 0 37 195 41 649.0 0 41 196 29 649.0 0 29 197 35 649.0 0 35 198 25 649.0 0 25 199 38 649.0 0 38 200 47 649.0 0 47 201 37 649.0 0 37 202 43 649.0 0 43 203 64 649.0 0 64 204 214 649.0 0 214 205 86 649.0 0 86 206 64 649.0 0 64 207 44 649.0 0 44 208 50 649.0 0 50 209 51 649.0 0 51 210 41 649.0 0 41 211 50 649.0 0 50 212 35 649.0 0 35 213 62 649.0 0 62 214 49 649.0 0 49 215 42 649.0 0 42 216 37 649.0 0 37 217 35 649.0 0 35 218 36 649.0 0 36 219 63 649.0 0 63 220 67 649.0 0 67 221 55 649.0 0 55 222 51 649.0 0 51 223 73 649.0 0 73 224 78 649.0 0 78 225 59 649.0 0 59 226 76 649.0 0 76 227 53 649.0 0 53 228 48 649.0 0 48 229 92 649.0 0 92 230 61 649.0 0 61 231 73 649.0 0 73 232 67 649.0 0 67 233 40 649.0 0 40 234 34 649.0 0 34 235 39 649.0 0 39 236 42 649.0 0 42 237 74 649.0 0 74 238 65 649.0 0 65 239 80 649.0 0 80 240 49 649.0 0 49 241 46 649.0 0 46 242 47 649.0 0 47 243 55 649.0 0 55 244 43 649.0 0 43 245 34 649.0 0 34 246 64 649.0 0 64 247 46 649.0 0 46 248 58 649.0 0 58 249 41 649.0 0 41 250 40 649.0 0 40 251 57 649.0 0 57 252 62 649.0 0 62 253 76 649.0 0 76 254 149 649.0 0 149 255 147 649.0 0 147 256 138 649.0 0 138 257 93 649.0 0 93 258 71 649.0 0 71 259 77 649.0 0 77 260 61 649.0 0 61 261 94 649.0 0 94 262 197 649.0 0 197 263 202 649.0 0 202 264 166 649.0 0 166 265 41 649.0 0 41 266 58 649.0 0 58 267 74 649.0 0 74 268 69 649.0 0 69 269 56 649.0 0 56 270 48 649.0 0 48 271 66 649.0 0 66 272 80 649.0 0 80 273 110 649.0 0 110 274 96 649.0 0 96 275 87 649.0 0 87 276 50 649.0 0 50 277 56 649.0 0 56 278 117 649.0 0 117 279 141 649.0 0 141 280 144 649.0 0 144 281 131 649.0 0 131 282 123 649.0 0 123 283 180 649.0 0 180 284 177 649.0 0 177 285 87 649.0 0 87 286 66 649.0 0 66 287 61 649.0 0 61 288 59 649.0 0 59 289 82 649.0 0 82 290 89 649.0 0 89 291 118 649.0 0 118 292 125 649.0 0 125 293 122 649.0 0 122 294 69 649.0 0 69 295 55 649.0 0 55 296 52 649.0 0 52 297 74 649.0 0 74 298 44 649.0 0 44 299 88 649.0 0 88 300 122 649.0 0 122 301 112 649.0 0 112 Finished in 2.70 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 53,884 Reads with adapters: 9,369 (17.4%) Reads that were too short: 28 (0.1%) Reads written (passing filters): 9,341 (17.3%) Total basepairs processed: 13,271,775 bp Total written (filtered): 2,543,485 bp (19.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9369 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 5 1 1 19 99 0.0 3 0 88 6 4 1 20 9042 0.0 4 8638 330 60 10 4 21 20 0.0 4 1 18 1 22 8 0.0 4 4 0 2 2 23 10 0.0 4 0 1 0 7 2 24 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 35 3 0.0 4 0 0 0 0 3 39 1 0.0 4 1 53 2 0.0 4 0 0 0 0 2 59 1 0.0 4 1 75 2 0.0 4 0 0 0 0 2 76 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 3 0.0 4 0 0 0 0 3 126 3 0.0 4 0 0 0 0 3 127 60 0.0 4 0 0 0 0 60 129 25 0.0 4 0 0 0 0 25 134 2 0.0 4 0 0 0 0 2 135 52 0.0 4 0 0 0 0 52 142 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 3 0.0 4 0 0 0 1 2 230 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 9,341 Reads with adapters: 574 (6.1%) Reads written (passing filters): 9,341 (100.0%) Total basepairs processed: 2,543,485 bp Total written (filtered): 2,536,413 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 574 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.6% C: 13.4% G: 77.7% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 41 146.0 0 41 4 5 36.5 0 5 9 4 0.0 0 0 4 10 15 0.0 1 1 14 11 379 0.0 1 8 371 12 112 0.0 1 3 109 13 4 0.0 1 0 4 20 7 0.0 1 3 4 113 3 0.0 1 3 127 1 0.0 1 1 152 1 0.0 1 1 210 2 0.0 1 1 1