This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.57 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 57,075 (2.1%) Reads that were too short: 5,281 (0.2%) Reads written (passing filters): 51,794 (1.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 13,007,460 bp (1.6%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 57075 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 994 649.0 0 994 7 13100 649.0 0 13100 8 302 649.0 0 302 9 8838 649.0 0 8838 10 22 649.0 0 22 11 17 649.0 0 17 12 14 649.0 0 14 13 18 649.0 0 18 14 14 649.0 0 14 15 17 649.0 0 17 16 15 649.0 0 15 17 26 649.0 0 26 18 19 649.0 0 19 19 19 649.0 0 19 20 18 649.0 0 18 21 11 649.0 0 11 22 16 649.0 0 16 23 28 649.0 0 28 24 30 649.0 0 30 25 20 649.0 0 20 26 13 649.0 0 13 27 36 649.0 0 36 28 49 649.0 0 49 29 31 649.0 0 31 30 38 649.0 0 38 31 17 649.0 0 17 32 18 649.0 0 18 33 57 649.0 0 57 34 84 649.0 0 84 35 124 649.0 0 124 36 25 649.0 0 25 37 42 649.0 0 42 38 26 649.0 0 26 39 25 649.0 0 25 40 36 649.0 0 36 41 26 649.0 0 26 42 31 649.0 0 31 43 46 649.0 0 46 44 80 649.0 0 80 45 62 649.0 0 62 46 38 649.0 0 38 47 32 649.0 0 32 48 50 649.0 0 50 49 45 649.0 0 45 50 103 649.0 0 103 51 96 649.0 0 96 52 161 649.0 0 161 53 237 649.0 0 237 54 3429 649.0 0 3429 55 4726 649.0 0 4726 56 4203 649.0 0 4203 57 31 649.0 0 31 58 17 649.0 0 17 59 16 649.0 0 16 60 19 649.0 0 19 61 19 649.0 0 19 62 16 649.0 0 16 63 19 649.0 0 19 64 17 649.0 0 17 65 19 649.0 0 19 66 17 649.0 0 17 67 19 649.0 0 19 68 21 649.0 0 21 69 31 649.0 0 31 70 23 649.0 0 23 71 19 649.0 0 19 72 18 649.0 0 18 73 19 649.0 0 19 74 22 649.0 0 22 75 26 649.0 0 26 76 12 649.0 0 12 77 26 649.0 0 26 78 12 649.0 0 12 79 22 649.0 0 22 80 38 649.0 0 38 81 67 649.0 0 67 82 39 649.0 0 39 83 37 649.0 0 37 84 47 649.0 0 47 85 23 649.0 0 23 86 55 649.0 0 55 87 54 649.0 0 54 88 67 649.0 0 67 89 48 649.0 0 48 90 44 649.0 0 44 91 79 649.0 0 79 92 70 649.0 0 70 93 142 649.0 0 142 94 3916 649.0 0 3916 95 998 649.0 0 998 96 2241 649.0 0 2241 97 30 649.0 0 30 98 24 649.0 0 24 99 26 649.0 0 26 100 26 649.0 0 26 101 22 649.0 0 22 102 24 649.0 0 24 103 31 649.0 0 31 104 24 649.0 0 24 105 36 649.0 0 36 106 19 649.0 0 19 107 28 649.0 0 28 108 23 649.0 0 23 109 102 649.0 0 102 110 86 649.0 0 86 111 151 649.0 0 151 112 16 649.0 0 16 113 29 649.0 0 29 114 35 649.0 0 35 115 32 649.0 0 32 116 30 649.0 0 30 117 49 649.0 0 49 118 45 649.0 0 45 119 59 649.0 0 59 120 68 649.0 0 68 121 286 649.0 0 286 122 215 649.0 0 215 123 233 649.0 0 233 124 20 649.0 0 20 125 40 649.0 0 40 126 37 649.0 0 37 127 32 649.0 0 32 128 30 649.0 0 30 129 37 649.0 0 37 130 38 649.0 0 38 131 35 649.0 0 35 132 23 649.0 0 23 133 51 649.0 0 51 134 34 649.0 0 34 135 43 649.0 0 43 136 60 649.0 0 60 137 68 649.0 0 68 138 44 649.0 0 44 139 63 649.0 0 63 140 57 649.0 0 57 141 107 649.0 0 107 142 38 649.0 0 38 143 39 649.0 0 39 144 42 649.0 0 42 145 84 649.0 0 84 146 35 649.0 0 35 147 81 649.0 0 81 148 39 649.0 0 39 149 117 649.0 0 117 150 47 649.0 0 47 151 45 649.0 0 45 152 40 649.0 0 40 153 68 649.0 0 68 154 62 649.0 0 62 155 30 649.0 0 30 156 37 649.0 0 37 157 43 649.0 0 43 158 41 649.0 0 41 159 56 649.0 0 56 160 193 649.0 0 193 161 167 649.0 0 167 162 90 649.0 0 90 163 41 649.0 0 41 164 29 649.0 0 29 165 48 649.0 0 48 166 66 649.0 0 66 167 51 649.0 0 51 168 43 649.0 0 43 169 31 649.0 0 31 170 26 649.0 0 26 171 23 649.0 0 23 172 32 649.0 0 32 173 46 649.0 0 46 174 41 649.0 0 41 175 93 649.0 0 93 176 122 649.0 0 122 177 99 649.0 0 99 178 74 649.0 0 74 179 36 649.0 0 36 180 54 649.0 0 54 181 112 649.0 0 112 182 67 649.0 0 67 183 31 649.0 0 31 184 37 649.0 0 37 185 39 649.0 0 39 186 54 649.0 0 54 187 32 649.0 0 32 188 56 649.0 0 56 189 39 649.0 0 39 190 30 649.0 0 30 191 36 649.0 0 36 192 36 649.0 0 36 193 58 649.0 0 58 194 59 649.0 0 59 195 35 649.0 0 35 196 43 649.0 0 43 197 36 649.0 0 36 198 30 649.0 0 30 199 32 649.0 0 32 200 42 649.0 0 42 201 24 649.0 0 24 202 35 649.0 0 35 203 46 649.0 0 46 204 174 649.0 0 174 205 42 649.0 0 42 206 31 649.0 0 31 207 32 649.0 0 32 208 37 649.0 0 37 209 36 649.0 0 36 210 36 649.0 0 36 211 36 649.0 0 36 212 32 649.0 0 32 213 48 649.0 0 48 214 49 649.0 0 49 215 38 649.0 0 38 216 37 649.0 0 37 217 32 649.0 0 32 218 33 649.0 0 33 219 48 649.0 0 48 220 49 649.0 0 49 221 52 649.0 0 52 222 40 649.0 0 40 223 39 649.0 0 39 224 40 649.0 0 40 225 35 649.0 0 35 226 48 649.0 0 48 227 51 649.0 0 51 228 53 649.0 0 53 229 53 649.0 0 53 230 42 649.0 0 42 231 47 649.0 0 47 232 46 649.0 0 46 233 35 649.0 0 35 234 41 649.0 0 41 235 31 649.0 0 31 236 48 649.0 0 48 237 70 649.0 0 70 238 56 649.0 0 56 239 68 649.0 0 68 240 40 649.0 0 40 241 35 649.0 0 35 242 48 649.0 0 48 243 40 649.0 0 40 244 36 649.0 0 36 245 30 649.0 0 30 246 34 649.0 0 34 247 44 649.0 0 44 248 50 649.0 0 50 249 48 649.0 0 48 250 45 649.0 0 45 251 33 649.0 0 33 252 51 649.0 0 51 253 41 649.0 0 41 254 56 649.0 0 56 255 69 649.0 0 69 256 58 649.0 0 58 257 66 649.0 0 66 258 42 649.0 0 42 259 39 649.0 0 39 260 44 649.0 0 44 261 60 649.0 0 60 262 125 649.0 0 125 263 106 649.0 0 106 264 104 649.0 0 104 265 44 649.0 0 44 266 36 649.0 0 36 267 49 649.0 0 49 268 43 649.0 0 43 269 35 649.0 0 35 270 54 649.0 0 54 271 39 649.0 0 39 272 32 649.0 0 32 273 82 649.0 0 82 274 79 649.0 0 79 275 75 649.0 0 75 276 51 649.0 0 51 277 33 649.0 0 33 278 67 649.0 0 67 279 71 649.0 0 71 280 104 649.0 0 104 281 55 649.0 0 55 282 65 649.0 0 65 283 95 649.0 0 95 284 73 649.0 0 73 285 43 649.0 0 43 286 27 649.0 0 27 287 45 649.0 0 45 288 54 649.0 0 54 289 48 649.0 0 48 290 51 649.0 0 51 291 96 649.0 0 96 292 92 649.0 0 92 293 65 649.0 0 65 294 59 649.0 0 59 295 45 649.0 0 45 296 59 649.0 0 59 297 49 649.0 0 49 298 42 649.0 0 42 299 61 649.0 0 61 300 90 649.0 0 90 301 63 649.0 0 63 Finished in 2.39 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 51,794 Reads with adapters: 13,235 (25.6%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 13,221 (25.5%) Total basepairs processed: 13,007,460 bp Total written (filtered): 3,589,974 bp (27.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13235 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 62 0.0 3 0 59 3 17 9054 0.0 3 8859 186 9 18 44 0.0 3 4 39 1 19 3736 0.0 3 3637 86 12 1 20 23 0.0 3 0 8 0 15 21 6 0.0 3 0 0 6 22 3 0.0 3 0 0 0 3 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 7 0.0 3 0 0 0 7 29 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 8 0.0 3 0 0 0 8 51 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 72 31 0.0 3 0 0 0 31 74 1 0.0 3 0 0 1 75 6 0.0 3 0 0 0 6 97 2 0.0 3 0 0 0 2 98 227 0.0 3 0 0 1 226 122 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 2 0.0 3 0 1 0 1 200 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 13,221 Reads with adapters: 699 (5.3%) Reads written (passing filters): 13,221 (100.0%) Total basepairs processed: 3,589,974 bp Total written (filtered): 3,587,852 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 699 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.8% C: 6.3% G: 14.9% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 682 206.6 0 682 4 14 51.6 0 14 5 2 12.9 0 2 10 1 0.0 1 0 1