This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.49 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 138,625 (5.2%) Reads that were too short: 19,550 (0.7%) Reads written (passing filters): 119,075 (4.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 30,322,754 bp (3.8%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 138625 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 679 649.0 0 679 7 1179 649.0 0 1179 8 41028 649.0 0 41028 9 35164 649.0 0 35164 10 75 649.0 0 75 11 49 649.0 0 49 12 32 649.0 0 32 13 61 649.0 0 61 14 29 649.0 0 29 15 29 649.0 0 29 16 27 649.0 0 27 17 49 649.0 0 49 18 38 649.0 0 38 19 24 649.0 0 24 20 35 649.0 0 35 21 36 649.0 0 36 22 43 649.0 0 43 23 40 649.0 0 40 24 37 649.0 0 37 25 38 649.0 0 38 26 34 649.0 0 34 27 39 649.0 0 39 28 57 649.0 0 57 29 35 649.0 0 35 30 30 649.0 0 30 31 63 649.0 0 63 32 80 649.0 0 80 33 39 649.0 0 39 34 32 649.0 0 32 35 47 649.0 0 47 36 120 649.0 0 120 37 26 649.0 0 26 38 44 649.0 0 44 39 50 649.0 0 50 40 50 649.0 0 50 41 97 649.0 0 97 42 158 649.0 0 158 43 72 649.0 0 72 44 107 649.0 0 107 45 139 649.0 0 139 46 63 649.0 0 63 47 130 649.0 0 130 48 37 649.0 0 37 49 48 649.0 0 48 50 83 649.0 0 83 51 54 649.0 0 54 52 38 649.0 0 38 53 34 649.0 0 34 54 24 649.0 0 24 55 34 649.0 0 34 56 35 649.0 0 35 57 61 649.0 0 61 58 53 649.0 0 53 59 55 649.0 0 55 60 49 649.0 0 49 61 38 649.0 0 38 62 60 649.0 0 60 63 43 649.0 0 43 64 43 649.0 0 43 65 154 649.0 0 154 66 105 649.0 0 105 67 52 649.0 0 52 68 91 649.0 0 91 69 72 649.0 0 72 70 113 649.0 0 113 71 124 649.0 0 124 72 192 649.0 0 192 73 190 649.0 0 190 74 979 649.0 0 979 75 438 649.0 0 438 76 431 649.0 0 431 77 455 649.0 0 455 78 279 649.0 0 279 79 2166 649.0 0 2166 80 1745 649.0 0 1745 81 2407 649.0 0 2407 82 320 649.0 0 320 83 337 649.0 0 337 84 439 649.0 0 439 85 82 649.0 0 82 86 128 649.0 0 128 87 105 649.0 0 105 88 88 649.0 0 88 89 63 649.0 0 63 90 82 649.0 0 82 91 51 649.0 0 51 92 68 649.0 0 68 93 88 649.0 0 88 94 91 649.0 0 91 95 85 649.0 0 85 96 91 649.0 0 91 97 214 649.0 0 214 98 131 649.0 0 131 99 148 649.0 0 148 100 374 649.0 0 374 101 306 649.0 0 306 102 97 649.0 0 97 103 141 649.0 0 141 104 190 649.0 0 190 105 165 649.0 0 165 106 151 649.0 0 151 107 193 649.0 0 193 108 192 649.0 0 192 109 156 649.0 0 156 110 139 649.0 0 139 111 188 649.0 0 188 112 256 649.0 0 256 113 219 649.0 0 219 114 236 649.0 0 236 115 284 649.0 0 284 116 275 649.0 0 275 117 245 649.0 0 245 118 236 649.0 0 236 119 224 649.0 0 224 120 243 649.0 0 243 121 255 649.0 0 255 122 215 649.0 0 215 123 271 649.0 0 271 124 415 649.0 0 415 125 1115 649.0 0 1115 126 560 649.0 0 560 127 945 649.0 0 945 128 257 649.0 0 257 129 384 649.0 0 384 130 295 649.0 0 295 131 187 649.0 0 187 132 186 649.0 0 186 133 142 649.0 0 142 134 188 649.0 0 188 135 185 649.0 0 185 136 264 649.0 0 264 137 442 649.0 0 442 138 474 649.0 0 474 139 469 649.0 0 469 140 275 649.0 0 275 141 697 649.0 0 697 142 402 649.0 0 402 143 835 649.0 0 835 144 151 649.0 0 151 145 169 649.0 0 169 146 158 649.0 0 158 147 178 649.0 0 178 148 144 649.0 0 144 149 158 649.0 0 158 150 210 649.0 0 210 151 152 649.0 0 152 152 193 649.0 0 193 153 111 649.0 0 111 154 128 649.0 0 128 155 120 649.0 0 120 156 144 649.0 0 144 157 162 649.0 0 162 158 475 649.0 0 475 159 323 649.0 0 323 160 346 649.0 0 346 161 157 649.0 0 157 162 114 649.0 0 114 163 157 649.0 0 157 164 186 649.0 0 186 165 159 649.0 0 159 166 155 649.0 0 155 167 118 649.0 0 118 168 120 649.0 0 120 169 144 649.0 0 144 170 131 649.0 0 131 171 126 649.0 0 126 172 133 649.0 0 133 173 112 649.0 0 112 174 117 649.0 0 117 175 101 649.0 0 101 176 108 649.0 0 108 177 144 649.0 0 144 178 164 649.0 0 164 179 140 649.0 0 140 180 131 649.0 0 131 181 210 649.0 0 210 182 1301 649.0 0 1301 183 1137 649.0 0 1137 184 846 649.0 0 846 185 157 649.0 0 157 186 100 649.0 0 100 187 121 649.0 0 121 188 121 649.0 0 121 189 131 649.0 0 131 190 102 649.0 0 102 191 104 649.0 0 104 192 97 649.0 0 97 193 114 649.0 0 114 194 107 649.0 0 107 195 113 649.0 0 113 196 119 649.0 0 119 197 112 649.0 0 112 198 131 649.0 0 131 199 101 649.0 0 101 200 133 649.0 0 133 201 114 649.0 0 114 202 120 649.0 0 120 203 133 649.0 0 133 204 136 649.0 0 136 205 142 649.0 0 142 206 182 649.0 0 182 207 145 649.0 0 145 208 192 649.0 0 192 209 152 649.0 0 152 210 150 649.0 0 150 211 178 649.0 0 178 212 157 649.0 0 157 213 101 649.0 0 101 214 126 649.0 0 126 215 114 649.0 0 114 216 119 649.0 0 119 217 101 649.0 0 101 218 130 649.0 0 130 219 119 649.0 0 119 220 133 649.0 0 133 221 132 649.0 0 132 222 113 649.0 0 113 223 124 649.0 0 124 224 142 649.0 0 142 225 173 649.0 0 173 226 191 649.0 0 191 227 195 649.0 0 195 228 120 649.0 0 120 229 105 649.0 0 105 230 102 649.0 0 102 231 171 649.0 0 171 232 149 649.0 0 149 233 129 649.0 0 129 234 116 649.0 0 116 235 127 649.0 0 127 236 151 649.0 0 151 237 404 649.0 0 404 238 480 649.0 0 480 239 325 649.0 0 325 240 288 649.0 0 288 241 143 649.0 0 143 242 140 649.0 0 140 243 101 649.0 0 101 244 97 649.0 0 97 245 112 649.0 0 112 246 126 649.0 0 126 247 106 649.0 0 106 248 126 649.0 0 126 249 153 649.0 0 153 250 158 649.0 0 158 251 131 649.0 0 131 252 189 649.0 0 189 253 1082 649.0 0 1082 254 1387 649.0 0 1387 255 849 649.0 0 849 256 585 649.0 0 585 257 156 649.0 0 156 258 122 649.0 0 122 259 149 649.0 0 149 260 135 649.0 0 135 261 146 649.0 0 146 262 131 649.0 0 131 263 151 649.0 0 151 264 385 649.0 0 385 265 251 649.0 0 251 266 371 649.0 0 371 267 83 649.0 0 83 268 94 649.0 0 94 269 133 649.0 0 133 270 114 649.0 0 114 271 120 649.0 0 120 272 137 649.0 0 137 273 145 649.0 0 145 274 146 649.0 0 146 275 215 649.0 0 215 276 175 649.0 0 175 277 224 649.0 0 224 278 199 649.0 0 199 279 139 649.0 0 139 280 138 649.0 0 138 281 153 649.0 0 153 282 205 649.0 0 205 283 150 649.0 0 150 284 146 649.0 0 146 285 125 649.0 0 125 286 147 649.0 0 147 287 113 649.0 0 113 288 236 649.0 0 236 289 284 649.0 0 284 290 332 649.0 0 332 291 203 649.0 0 203 292 98 649.0 0 98 293 133 649.0 0 133 294 107 649.0 0 107 295 128 649.0 0 128 296 163 649.0 0 163 297 186 649.0 0 186 298 163 649.0 0 163 299 171 649.0 0 171 300 137 649.0 0 137 301 259 649.0 0 259 Finished in 5.99 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 119,075 Reads with adapters: 37,320 (31.3%) Reads that were too short: 72 (0.1%) Reads written (passing filters): 37,248 (31.3%) Total basepairs processed: 30,322,754 bp Total written (filtered): 10,106,427 bp (33.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37320 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 1 5 18 25 0.0 3 0 0 15 7 3 19 460 0.0 3 0 423 17 14 6 20 36272 0.0 4 34555 1400 235 67 15 21 74 0.0 4 12 58 2 1 1 22 6 0.0 4 1 1 2 1 1 23 147 0.0 4 1 0 0 122 24 24 1 0.0 4 1 27 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 1 51 2 0.0 4 0 0 0 1 1 53 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 8 0.0 4 0 0 0 0 8 78 4 0.0 4 0 0 0 0 4 80 1 0.0 4 0 0 0 1 81 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 227 0.0 4 0 0 0 0 227 128 3 0.0 4 0 0 0 0 3 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 135 4 0.0 4 0 0 0 0 4 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 162 4 0.0 4 0 0 0 0 4 164 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 237 2 0.0 4 0 0 0 0 2 255 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.38 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 37,248 Reads with adapters: 9,193 (24.7%) Reads written (passing filters): 37,248 (100.0%) Total basepairs processed: 10,106,427 bp Total written (filtered): 10,001,601 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9193 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 8.8% G: 87.4% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 582.0 0 17 4 7 145.5 0 7 5 1 36.4 0 1 7 1 2.3 0 1 8 5 0.6 0 5 9 3310 0.1 0 335 2975 10 4264 0.0 1 276 3988 11 1468 0.0 1 44 1424 12 29 0.0 1 0 29 13 15 0.0 1 1 14 14 1 0.0 1 0 1 15 1 0.0 1 0 1 25 1 0.0 1 1 80 1 0.0 1 0 1 150 6 0.0 1 4 2 208 11 0.0 1 5 6 210 1 0.0 1 1 223 54 0.0 1 48 6 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.