This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.56 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 149,277 (5.6%) Reads that were too short: 23,706 (0.9%) Reads written (passing filters): 125,571 (4.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 31,673,063 bp (4.0%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 149277 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 473 649.0 0 473 7 767 649.0 0 767 8 38111 649.0 0 38111 9 40772 649.0 0 40772 10 84 649.0 0 84 11 64 649.0 0 64 12 51 649.0 0 51 13 57 649.0 0 57 14 45 649.0 0 45 15 47 649.0 0 47 16 47 649.0 0 47 17 54 649.0 0 54 18 46 649.0 0 46 19 57 649.0 0 57 20 45 649.0 0 45 21 56 649.0 0 56 22 59 649.0 0 59 23 74 649.0 0 74 24 47 649.0 0 47 25 66 649.0 0 66 26 78 649.0 0 78 27 62 649.0 0 62 28 70 649.0 0 70 29 64 649.0 0 64 30 55 649.0 0 55 31 64 649.0 0 64 32 71 649.0 0 71 33 35 649.0 0 35 34 48 649.0 0 48 35 71 649.0 0 71 36 177 649.0 0 177 37 59 649.0 0 59 38 194 649.0 0 194 39 65 649.0 0 65 40 69 649.0 0 69 41 109 649.0 0 109 42 160 649.0 0 160 43 98 649.0 0 98 44 160 649.0 0 160 45 133 649.0 0 133 46 84 649.0 0 84 47 100 649.0 0 100 48 66 649.0 0 66 49 65 649.0 0 65 50 102 649.0 0 102 51 76 649.0 0 76 52 50 649.0 0 50 53 40 649.0 0 40 54 51 649.0 0 51 55 44 649.0 0 44 56 58 649.0 0 58 57 112 649.0 0 112 58 83 649.0 0 83 59 62 649.0 0 62 60 69 649.0 0 69 61 48 649.0 0 48 62 65 649.0 0 65 63 93 649.0 0 93 64 67 649.0 0 67 65 200 649.0 0 200 66 157 649.0 0 157 67 87 649.0 0 87 68 114 649.0 0 114 69 92 649.0 0 92 70 126 649.0 0 126 71 112 649.0 0 112 72 105 649.0 0 105 73 89 649.0 0 89 74 112 649.0 0 112 75 86 649.0 0 86 76 109 649.0 0 109 77 152 649.0 0 152 78 194 649.0 0 194 79 2352 649.0 0 2352 80 1951 649.0 0 1951 81 2567 649.0 0 2567 82 391 649.0 0 391 83 347 649.0 0 347 84 638 649.0 0 638 85 209 649.0 0 209 86 198 649.0 0 198 87 171 649.0 0 171 88 331 649.0 0 331 89 166 649.0 0 166 90 206 649.0 0 206 91 243 649.0 0 243 92 213 649.0 0 213 93 174 649.0 0 174 94 170 649.0 0 170 95 213 649.0 0 213 96 191 649.0 0 191 97 233 649.0 0 233 98 275 649.0 0 275 99 240 649.0 0 240 100 191 649.0 0 191 101 206 649.0 0 206 102 253 649.0 0 253 103 219 649.0 0 219 104 203 649.0 0 203 105 169 649.0 0 169 106 176 649.0 0 176 107 223 649.0 0 223 108 283 649.0 0 283 109 222 649.0 0 222 110 227 649.0 0 227 111 197 649.0 0 197 112 212 649.0 0 212 113 190 649.0 0 190 114 251 649.0 0 251 115 286 649.0 0 286 116 285 649.0 0 285 117 315 649.0 0 315 118 269 649.0 0 269 119 236 649.0 0 236 120 274 649.0 0 274 121 261 649.0 0 261 122 223 649.0 0 223 123 265 649.0 0 265 124 375 649.0 0 375 125 731 649.0 0 731 126 414 649.0 0 414 127 709 649.0 0 709 128 287 649.0 0 287 129 376 649.0 0 376 130 316 649.0 0 316 131 252 649.0 0 252 132 216 649.0 0 216 133 179 649.0 0 179 134 218 649.0 0 218 135 224 649.0 0 224 136 331 649.0 0 331 137 464 649.0 0 464 138 542 649.0 0 542 139 443 649.0 0 443 140 283 649.0 0 283 141 694 649.0 0 694 142 515 649.0 0 515 143 912 649.0 0 912 144 216 649.0 0 216 145 176 649.0 0 176 146 171 649.0 0 171 147 181 649.0 0 181 148 183 649.0 0 183 149 183 649.0 0 183 150 245 649.0 0 245 151 227 649.0 0 227 152 212 649.0 0 212 153 179 649.0 0 179 154 169 649.0 0 169 155 176 649.0 0 176 156 207 649.0 0 207 157 179 649.0 0 179 158 566 649.0 0 566 159 406 649.0 0 406 160 424 649.0 0 424 161 175 649.0 0 175 162 182 649.0 0 182 163 155 649.0 0 155 164 277 649.0 0 277 165 200 649.0 0 200 166 179 649.0 0 179 167 165 649.0 0 165 168 183 649.0 0 183 169 158 649.0 0 158 170 203 649.0 0 203 171 204 649.0 0 204 172 186 649.0 0 186 173 151 649.0 0 151 174 135 649.0 0 135 175 184 649.0 0 184 176 150 649.0 0 150 177 198 649.0 0 198 178 197 649.0 0 197 179 190 649.0 0 190 180 173 649.0 0 173 181 255 649.0 0 255 182 1057 649.0 0 1057 183 1173 649.0 0 1173 184 870 649.0 0 870 185 187 649.0 0 187 186 132 649.0 0 132 187 166 649.0 0 166 188 181 649.0 0 181 189 171 649.0 0 171 190 152 649.0 0 152 191 191 649.0 0 191 192 136 649.0 0 136 193 145 649.0 0 145 194 156 649.0 0 156 195 188 649.0 0 188 196 169 649.0 0 169 197 200 649.0 0 200 198 188 649.0 0 188 199 184 649.0 0 184 200 204 649.0 0 204 201 191 649.0 0 191 202 173 649.0 0 173 203 198 649.0 0 198 204 176 649.0 0 176 205 197 649.0 0 197 206 203 649.0 0 203 207 213 649.0 0 213 208 213 649.0 0 213 209 202 649.0 0 202 210 191 649.0 0 191 211 201 649.0 0 201 212 211 649.0 0 211 213 153 649.0 0 153 214 153 649.0 0 153 215 186 649.0 0 186 216 151 649.0 0 151 217 178 649.0 0 178 218 151 649.0 0 151 219 168 649.0 0 168 220 140 649.0 0 140 221 189 649.0 0 189 222 167 649.0 0 167 223 174 649.0 0 174 224 205 649.0 0 205 225 204 649.0 0 204 226 241 649.0 0 241 227 221 649.0 0 221 228 166 649.0 0 166 229 152 649.0 0 152 230 141 649.0 0 141 231 204 649.0 0 204 232 146 649.0 0 146 233 154 649.0 0 154 234 121 649.0 0 121 235 173 649.0 0 173 236 199 649.0 0 199 237 333 649.0 0 333 238 369 649.0 0 369 239 256 649.0 0 256 240 228 649.0 0 228 241 175 649.0 0 175 242 170 649.0 0 170 243 165 649.0 0 165 244 125 649.0 0 125 245 157 649.0 0 157 246 163 649.0 0 163 247 162 649.0 0 162 248 169 649.0 0 169 249 177 649.0 0 177 250 211 649.0 0 211 251 207 649.0 0 207 252 253 649.0 0 253 253 1631 649.0 0 1631 254 2547 649.0 0 2547 255 1325 649.0 0 1325 256 1158 649.0 0 1158 257 199 649.0 0 199 258 117 649.0 0 117 259 168 649.0 0 168 260 146 649.0 0 146 261 166 649.0 0 166 262 160 649.0 0 160 263 162 649.0 0 162 264 231 649.0 0 231 265 199 649.0 0 199 266 239 649.0 0 239 267 99 649.0 0 99 268 142 649.0 0 142 269 159 649.0 0 159 270 122 649.0 0 122 271 137 649.0 0 137 272 141 649.0 0 141 273 149 649.0 0 149 274 188 649.0 0 188 275 191 649.0 0 191 276 172 649.0 0 172 277 215 649.0 0 215 278 157 649.0 0 157 279 155 649.0 0 155 280 151 649.0 0 151 281 151 649.0 0 151 282 189 649.0 0 189 283 125 649.0 0 125 284 174 649.0 0 174 285 134 649.0 0 134 286 138 649.0 0 138 287 163 649.0 0 163 288 162 649.0 0 162 289 216 649.0 0 216 290 253 649.0 0 253 291 180 649.0 0 180 292 129 649.0 0 129 293 117 649.0 0 117 294 151 649.0 0 151 295 123 649.0 0 123 296 158 649.0 0 158 297 169 649.0 0 169 298 152 649.0 0 152 299 170 649.0 0 170 300 150 649.0 0 150 301 241 649.0 0 241 Finished in 5.54 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 125,571 Reads with adapters: 37,790 (30.1%) Reads that were too short: 101 (0.1%) Reads written (passing filters): 37,689 (30.0%) Total basepairs processed: 31,673,063 bp Total written (filtered): 10,379,573 bp (32.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 37790 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 5 0.0 3 0 0 4 1 16 175 0.0 3 0 168 7 17 37224 0.0 3 36467 729 24 4 18 73 0.0 3 6 63 3 1 19 25 0.0 3 1 0 24 20 38 0.0 3 0 0 0 38 21 1 0.0 3 1 27 1 0.0 3 0 0 0 1 28 4 0.0 3 1 0 0 3 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 1 1 40 1 0.0 3 0 0 0 1 41 4 0.0 3 0 0 0 4 42 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 1 2 45 1 0.0 3 0 0 0 1 51 13 0.0 3 0 0 1 12 52 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 58 0.0 3 0 0 2 56 67 8 0.0 3 0 0 0 8 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 112 3 0.0 3 0 0 0 3 123 6 0.0 3 0 0 0 6 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 7 0.0 3 0 0 1 6 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 1 1 141 1 0.0 3 0 0 0 1 145 7 0.0 3 0 0 0 7 149 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 47 0.0 3 0 0 0 47 218 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 238 1 0.0 3 0 1 244 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.33 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 37,689 Reads with adapters: 713 (1.9%) Reads written (passing filters): 37,689 (100.0%) Total basepairs processed: 10,379,573 bp Total written (filtered): 10,377,209 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 713 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.3% C: 6.3% G: 25.0% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 504 588.9 0 504 4 202 147.2 0 202 5 3 36.8 0 3 6 3 9.2 0 3 11 1 0.0 1 0 1