This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.05 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 60,472 (2.3%) Reads that were too short: 16,162 (0.6%) Reads written (passing filters): 44,310 (1.7%) Total basepairs processed: 800,111,879 bp Total written (filtered): 10,749,350 bp (1.3%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 60472 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 457 649.0 0 457 7 13090 649.0 0 13090 8 11462 649.0 0 11462 9 45 649.0 0 45 10 38 649.0 0 38 11 36 649.0 0 36 12 22 649.0 0 22 13 37 649.0 0 37 14 45 649.0 0 45 15 47 649.0 0 47 16 53 649.0 0 53 17 39 649.0 0 39 18 37 649.0 0 37 19 44 649.0 0 44 20 28 649.0 0 28 21 25 649.0 0 25 22 26 649.0 0 26 23 35 649.0 0 35 24 36 649.0 0 36 25 42 649.0 0 42 26 115 649.0 0 115 27 95 649.0 0 95 28 86 649.0 0 86 29 30 649.0 0 30 30 28 649.0 0 28 31 32 649.0 0 32 32 42 649.0 0 42 33 43 649.0 0 43 34 55 649.0 0 55 35 13 649.0 0 13 36 57 649.0 0 57 37 101 649.0 0 101 38 159 649.0 0 159 39 38 649.0 0 38 40 32 649.0 0 32 41 30 649.0 0 30 42 45 649.0 0 45 43 26 649.0 0 26 44 27 649.0 0 27 45 32 649.0 0 32 46 28 649.0 0 28 47 28 649.0 0 28 48 17 649.0 0 17 49 38 649.0 0 38 50 31 649.0 0 31 51 34 649.0 0 34 52 25 649.0 0 25 53 25 649.0 0 25 54 43 649.0 0 43 55 23 649.0 0 23 56 32 649.0 0 32 57 18 649.0 0 18 58 40 649.0 0 40 59 54 649.0 0 54 60 28 649.0 0 28 61 122 649.0 0 122 62 24 649.0 0 24 63 29 649.0 0 29 64 26 649.0 0 26 65 27 649.0 0 27 66 27 649.0 0 27 67 29 649.0 0 29 68 18 649.0 0 18 69 39 649.0 0 39 70 27 649.0 0 27 71 34 649.0 0 34 72 26 649.0 0 26 73 19 649.0 0 19 74 32 649.0 0 32 75 160 649.0 0 160 76 81 649.0 0 81 77 145 649.0 0 145 78 57 649.0 0 57 79 76 649.0 0 76 80 50 649.0 0 50 81 76 649.0 0 76 82 45 649.0 0 45 83 55 649.0 0 55 84 57 649.0 0 57 85 50 649.0 0 50 86 42 649.0 0 42 87 40 649.0 0 40 88 42 649.0 0 42 89 31 649.0 0 31 90 35 649.0 0 35 91 43 649.0 0 43 92 45 649.0 0 45 93 53 649.0 0 53 94 87 649.0 0 87 95 52 649.0 0 52 96 167 649.0 0 167 97 111 649.0 0 111 98 95 649.0 0 95 99 200 649.0 0 200 100 344 649.0 0 344 101 169 649.0 0 169 102 152 649.0 0 152 103 125 649.0 0 125 104 108 649.0 0 108 105 150 649.0 0 150 106 185 649.0 0 185 107 153 649.0 0 153 108 586 649.0 0 586 109 300 649.0 0 300 110 457 649.0 0 457 111 178 649.0 0 178 112 180 649.0 0 180 113 138 649.0 0 138 114 219 649.0 0 219 115 198 649.0 0 198 116 229 649.0 0 229 117 128 649.0 0 128 118 89 649.0 0 89 119 86 649.0 0 86 120 67 649.0 0 67 121 91 649.0 0 91 122 155 649.0 0 155 123 147 649.0 0 147 124 81 649.0 0 81 125 91 649.0 0 91 126 109 649.0 0 109 127 111 649.0 0 111 128 134 649.0 0 134 129 176 649.0 0 176 130 243 649.0 0 243 131 220 649.0 0 220 132 202 649.0 0 202 133 109 649.0 0 109 134 126 649.0 0 126 135 149 649.0 0 149 136 192 649.0 0 192 137 241 649.0 0 241 138 118 649.0 0 118 139 129 649.0 0 129 140 107 649.0 0 107 141 130 649.0 0 130 142 158 649.0 0 158 143 120 649.0 0 120 144 151 649.0 0 151 145 127 649.0 0 127 146 110 649.0 0 110 147 124 649.0 0 124 148 128 649.0 0 128 149 121 649.0 0 121 150 108 649.0 0 108 151 96 649.0 0 96 152 97 649.0 0 97 153 104 649.0 0 104 154 113 649.0 0 113 155 156 649.0 0 156 156 167 649.0 0 167 157 148 649.0 0 148 158 156 649.0 0 156 159 123 649.0 0 123 160 101 649.0 0 101 161 123 649.0 0 123 162 260 649.0 0 260 163 141 649.0 0 141 164 328 649.0 0 328 165 144 649.0 0 144 166 117 649.0 0 117 167 148 649.0 0 148 168 116 649.0 0 116 169 142 649.0 0 142 170 129 649.0 0 129 171 127 649.0 0 127 172 102 649.0 0 102 173 93 649.0 0 93 174 80 649.0 0 80 175 94 649.0 0 94 176 102 649.0 0 102 177 96 649.0 0 96 178 127 649.0 0 127 179 119 649.0 0 119 180 111 649.0 0 111 181 97 649.0 0 97 182 89 649.0 0 89 183 114 649.0 0 114 184 112 649.0 0 112 185 103 649.0 0 103 186 104 649.0 0 104 187 107 649.0 0 107 188 115 649.0 0 115 189 107 649.0 0 107 190 154 649.0 0 154 191 109 649.0 0 109 192 114 649.0 0 114 193 100 649.0 0 100 194 95 649.0 0 95 195 100 649.0 0 100 196 105 649.0 0 105 197 97 649.0 0 97 198 86 649.0 0 86 199 111 649.0 0 111 200 149 649.0 0 149 201 165 649.0 0 165 202 193 649.0 0 193 203 166 649.0 0 166 204 157 649.0 0 157 205 115 649.0 0 115 206 114 649.0 0 114 207 134 649.0 0 134 208 206 649.0 0 206 209 207 649.0 0 207 210 208 649.0 0 208 211 127 649.0 0 127 212 85 649.0 0 85 213 104 649.0 0 104 214 81 649.0 0 81 215 105 649.0 0 105 216 123 649.0 0 123 217 101 649.0 0 101 218 88 649.0 0 88 219 83 649.0 0 83 220 113 649.0 0 113 221 136 649.0 0 136 222 189 649.0 0 189 223 196 649.0 0 196 224 154 649.0 0 154 225 117 649.0 0 117 226 129 649.0 0 129 227 108 649.0 0 108 228 96 649.0 0 96 229 103 649.0 0 103 230 90 649.0 0 90 231 90 649.0 0 90 232 113 649.0 0 113 233 77 649.0 0 77 234 81 649.0 0 81 235 116 649.0 0 116 236 110 649.0 0 110 237 119 649.0 0 119 238 114 649.0 0 114 239 109 649.0 0 109 240 176 649.0 0 176 241 134 649.0 0 134 242 199 649.0 0 199 243 156 649.0 0 156 244 278 649.0 0 278 245 244 649.0 0 244 246 212 649.0 0 212 247 113 649.0 0 113 248 89 649.0 0 89 249 107 649.0 0 107 250 95 649.0 0 95 251 85 649.0 0 85 252 157 649.0 0 157 253 426 649.0 0 426 254 353 649.0 0 353 255 424 649.0 0 424 256 121 649.0 0 121 257 155 649.0 0 155 258 520 649.0 0 520 259 369 649.0 0 369 260 293 649.0 0 293 261 133 649.0 0 133 262 131 649.0 0 131 263 278 649.0 0 278 264 357 649.0 0 357 265 225 649.0 0 225 266 129 649.0 0 129 267 74 649.0 0 74 268 75 649.0 0 75 269 169 649.0 0 169 270 161 649.0 0 161 271 396 649.0 0 396 272 178 649.0 0 178 273 298 649.0 0 298 274 149 649.0 0 149 275 192 649.0 0 192 276 171 649.0 0 171 277 120 649.0 0 120 278 123 649.0 0 123 279 118 649.0 0 118 280 91 649.0 0 91 281 137 649.0 0 137 282 138 649.0 0 138 283 173 649.0 0 173 284 218 649.0 0 218 285 175 649.0 0 175 286 157 649.0 0 157 287 120 649.0 0 120 288 101 649.0 0 101 289 118 649.0 0 118 290 167 649.0 0 167 291 179 649.0 0 179 292 200 649.0 0 200 293 122 649.0 0 122 294 94 649.0 0 94 295 110 649.0 0 110 296 134 649.0 0 134 297 154 649.0 0 154 298 153 649.0 0 153 299 130 649.0 0 130 300 153 649.0 0 153 301 198 649.0 0 198 Finished in 2.20 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 44,310 Reads with adapters: 11,952 (27.0%) Reads that were too short: 30 (0.1%) Reads written (passing filters): 11,922 (26.9%) Total basepairs processed: 10,749,350 bp Total written (filtered): 3,232,297 bp (30.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11952 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 4 1 19 122 0.0 3 0 96 12 10 4 20 11502 0.0 4 10905 489 86 18 4 21 41 0.0 4 8 28 1 4 22 4 0.0 4 1 0 3 23 30 0.0 4 1 1 0 18 10 24 1 0.0 4 0 0 0 0 1 25 2 0.0 4 1 0 0 1 26 23 0.0 4 0 0 0 0 23 29 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 1 80 1 0.0 4 0 0 0 1 93 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 179 0.0 4 0 0 0 0 179 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 265 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 11,922 Reads with adapters: 2,250 (18.9%) Reads written (passing filters): 11,922 (100.0%) Total basepairs processed: 3,232,297 bp Total written (filtered): 3,205,443 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2250 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 7.1% G: 88.5% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 186.3 0 11 4 1 46.6 0 1 8 1 0.2 0 1 9 6 0.0 0 1 5 10 332 0.0 1 26 306 11 1862 0.0 1 67 1795 12 17 0.0 1 0 17 13 4 0.0 1 0 4 14 1 0.0 1 0 1 19 1 0.0 1 1 126 1 0.0 1 1 151 3 0.0 1 3 178 1 0.0 1 1 190 1 0.0 1 0 1 209 5 0.0 1 4 1 224 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.