This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.64 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 58,659 (2.2%) Reads that were too short: 15,411 (0.6%) Reads written (passing filters): 43,248 (1.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 10,560,647 bp (1.3%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 58659 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 257 649.0 0 257 7 12672 649.0 0 12672 8 12817 649.0 0 12817 9 15 649.0 0 15 10 29 649.0 0 29 11 14 649.0 0 14 12 17 649.0 0 17 13 28 649.0 0 28 14 15 649.0 0 15 15 20 649.0 0 20 16 30 649.0 0 30 17 22 649.0 0 22 18 20 649.0 0 20 19 23 649.0 0 23 20 13 649.0 0 13 21 15 649.0 0 15 22 15 649.0 0 15 23 20 649.0 0 20 24 19 649.0 0 19 25 15 649.0 0 15 26 21 649.0 0 21 27 16 649.0 0 16 28 25 649.0 0 25 29 7 649.0 0 7 30 14 649.0 0 14 31 18 649.0 0 18 32 13 649.0 0 13 33 24 649.0 0 24 34 32 649.0 0 32 35 17 649.0 0 17 36 39 649.0 0 39 37 84 649.0 0 84 38 133 649.0 0 133 39 28 649.0 0 28 40 23 649.0 0 23 41 14 649.0 0 14 42 266 649.0 0 266 43 22 649.0 0 22 44 21 649.0 0 21 45 24 649.0 0 24 46 28 649.0 0 28 47 18 649.0 0 18 48 17 649.0 0 17 49 20 649.0 0 20 50 21 649.0 0 21 51 21 649.0 0 21 52 17 649.0 0 17 53 13 649.0 0 13 54 31 649.0 0 31 55 13 649.0 0 13 56 20 649.0 0 20 57 19 649.0 0 19 58 21 649.0 0 21 59 15 649.0 0 15 60 23 649.0 0 23 61 93 649.0 0 93 62 14 649.0 0 14 63 11 649.0 0 11 64 19 649.0 0 19 65 18 649.0 0 18 66 18 649.0 0 18 67 28 649.0 0 28 68 12 649.0 0 12 69 26 649.0 0 26 70 16 649.0 0 16 71 19 649.0 0 19 72 27 649.0 0 27 73 13 649.0 0 13 74 8 649.0 0 8 75 18 649.0 0 18 76 16 649.0 0 16 77 26 649.0 0 26 78 20 649.0 0 20 79 12 649.0 0 12 80 47 649.0 0 47 81 69 649.0 0 69 82 60 649.0 0 60 83 58 649.0 0 58 84 77 649.0 0 77 85 84 649.0 0 84 86 54 649.0 0 54 87 65 649.0 0 65 88 57 649.0 0 57 89 75 649.0 0 75 90 66 649.0 0 66 91 75 649.0 0 75 92 96 649.0 0 96 93 86 649.0 0 86 94 110 649.0 0 110 95 73 649.0 0 73 96 111 649.0 0 111 97 141 649.0 0 141 98 131 649.0 0 131 99 159 649.0 0 159 100 141 649.0 0 141 101 116 649.0 0 116 102 104 649.0 0 104 103 91 649.0 0 91 104 84 649.0 0 84 105 120 649.0 0 120 106 194 649.0 0 194 107 176 649.0 0 176 108 385 649.0 0 385 109 269 649.0 0 269 110 456 649.0 0 456 111 226 649.0 0 226 112 223 649.0 0 223 113 163 649.0 0 163 114 266 649.0 0 266 115 217 649.0 0 217 116 268 649.0 0 268 117 110 649.0 0 110 118 113 649.0 0 113 119 64 649.0 0 64 120 65 649.0 0 65 121 88 649.0 0 88 122 153 649.0 0 153 123 143 649.0 0 143 124 100 649.0 0 100 125 81 649.0 0 81 126 89 649.0 0 89 127 89 649.0 0 89 128 120 649.0 0 120 129 183 649.0 0 183 130 190 649.0 0 190 131 221 649.0 0 221 132 180 649.0 0 180 133 102 649.0 0 102 134 100 649.0 0 100 135 117 649.0 0 117 136 178 649.0 0 178 137 251 649.0 0 251 138 141 649.0 0 141 139 124 649.0 0 124 140 74 649.0 0 74 141 113 649.0 0 113 142 146 649.0 0 146 143 114 649.0 0 114 144 112 649.0 0 112 145 112 649.0 0 112 146 91 649.0 0 91 147 89 649.0 0 89 148 124 649.0 0 124 149 129 649.0 0 129 150 120 649.0 0 120 151 98 649.0 0 98 152 124 649.0 0 124 153 113 649.0 0 113 154 108 649.0 0 108 155 153 649.0 0 153 156 140 649.0 0 140 157 165 649.0 0 165 158 171 649.0 0 171 159 108 649.0 0 108 160 126 649.0 0 126 161 113 649.0 0 113 162 251 649.0 0 251 163 184 649.0 0 184 164 329 649.0 0 329 165 168 649.0 0 168 166 125 649.0 0 125 167 125 649.0 0 125 168 106 649.0 0 106 169 126 649.0 0 126 170 129 649.0 0 129 171 97 649.0 0 97 172 87 649.0 0 87 173 67 649.0 0 67 174 91 649.0 0 91 175 91 649.0 0 91 176 133 649.0 0 133 177 102 649.0 0 102 178 122 649.0 0 122 179 92 649.0 0 92 180 123 649.0 0 123 181 108 649.0 0 108 182 112 649.0 0 112 183 98 649.0 0 98 184 97 649.0 0 97 185 82 649.0 0 82 186 116 649.0 0 116 187 81 649.0 0 81 188 119 649.0 0 119 189 116 649.0 0 116 190 134 649.0 0 134 191 126 649.0 0 126 192 115 649.0 0 115 193 89 649.0 0 89 194 118 649.0 0 118 195 99 649.0 0 99 196 97 649.0 0 97 197 85 649.0 0 85 198 105 649.0 0 105 199 77 649.0 0 77 200 120 649.0 0 120 201 140 649.0 0 140 202 221 649.0 0 221 203 159 649.0 0 159 204 137 649.0 0 137 205 116 649.0 0 116 206 108 649.0 0 108 207 123 649.0 0 123 208 166 649.0 0 166 209 199 649.0 0 199 210 194 649.0 0 194 211 135 649.0 0 135 212 74 649.0 0 74 213 95 649.0 0 95 214 90 649.0 0 90 215 97 649.0 0 97 216 113 649.0 0 113 217 106 649.0 0 106 218 111 649.0 0 111 219 97 649.0 0 97 220 99 649.0 0 99 221 101 649.0 0 101 222 152 649.0 0 152 223 197 649.0 0 197 224 155 649.0 0 155 225 93 649.0 0 93 226 108 649.0 0 108 227 109 649.0 0 109 228 89 649.0 0 89 229 97 649.0 0 97 230 103 649.0 0 103 231 68 649.0 0 68 232 113 649.0 0 113 233 87 649.0 0 87 234 93 649.0 0 93 235 104 649.0 0 104 236 107 649.0 0 107 237 130 649.0 0 130 238 111 649.0 0 111 239 104 649.0 0 104 240 142 649.0 0 142 241 150 649.0 0 150 242 240 649.0 0 240 243 170 649.0 0 170 244 243 649.0 0 243 245 239 649.0 0 239 246 207 649.0 0 207 247 108 649.0 0 108 248 96 649.0 0 96 249 97 649.0 0 97 250 108 649.0 0 108 251 106 649.0 0 106 252 169 649.0 0 169 253 374 649.0 0 374 254 307 649.0 0 307 255 341 649.0 0 341 256 115 649.0 0 115 257 147 649.0 0 147 258 572 649.0 0 572 259 368 649.0 0 368 260 331 649.0 0 331 261 130 649.0 0 130 262 135 649.0 0 135 263 235 649.0 0 235 264 477 649.0 0 477 265 225 649.0 0 225 266 199 649.0 0 199 267 73 649.0 0 73 268 76 649.0 0 76 269 131 649.0 0 131 270 132 649.0 0 132 271 326 649.0 0 326 272 168 649.0 0 168 273 287 649.0 0 287 274 132 649.0 0 132 275 211 649.0 0 211 276 116 649.0 0 116 277 128 649.0 0 128 278 95 649.0 0 95 279 79 649.0 0 79 280 87 649.0 0 87 281 133 649.0 0 133 282 127 649.0 0 127 283 165 649.0 0 165 284 142 649.0 0 142 285 139 649.0 0 139 286 122 649.0 0 122 287 76 649.0 0 76 288 102 649.0 0 102 289 129 649.0 0 129 290 162 649.0 0 162 291 160 649.0 0 160 292 172 649.0 0 172 293 109 649.0 0 109 294 87 649.0 0 87 295 97 649.0 0 97 296 128 649.0 0 128 297 152 649.0 0 152 298 178 649.0 0 178 299 132 649.0 0 132 300 116 649.0 0 116 301 150 649.0 0 150 Finished in 1.97 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 43,248 Reads with adapters: 12,776 (29.5%) Reads that were too short: 65 (0.2%) Reads written (passing filters): 12,711 (29.4%) Total basepairs processed: 10,560,647 bp Total written (filtered): 3,503,047 bp (33.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12776 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 64 0.0 3 0 62 2 17 12478 0.0 3 12237 233 6 2 18 37 0.0 3 2 34 1 19 5 0.0 3 0 0 2 3 20 9 0.0 3 0 0 0 9 21 1 0.0 3 1 23 2 0.0 3 0 0 0 2 24 5 0.0 3 0 0 0 5 26 1 0.0 3 0 0 0 1 29 4 0.0 3 0 0 0 4 31 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 60 82 0.0 3 0 0 1 81 61 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 1 1 70 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 165 1 0.0 3 0 1 173 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 37 0.0 3 0 0 2 35 221 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.45 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 12,711 Reads with adapters: 48 (0.4%) Reads written (passing filters): 12,711 (100.0%) Total basepairs processed: 3,503,047 bp Total written (filtered): 3,502,885 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 48 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.7% C: 31.2% G: 25.0% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 34 198.6 0 34 4 11 49.7 0 11 5 2 12.4 0 2 6 1 3.1 0 1