This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.35 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 103,543 (3.9%) Reads that were too short: 38,061 (1.4%) Reads written (passing filters): 65,482 (2.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 16,137,304 bp (2.0%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 103543 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2071 649.0 0 2071 7 15462 649.0 0 15462 8 603 649.0 0 603 9 19000 649.0 0 19000 10 63 649.0 0 63 11 49 649.0 0 49 12 54 649.0 0 54 13 45 649.0 0 45 14 54 649.0 0 54 15 45 649.0 0 45 16 51 649.0 0 51 17 109 649.0 0 109 18 122 649.0 0 122 19 101 649.0 0 101 20 41 649.0 0 41 21 68 649.0 0 68 22 30 649.0 0 30 23 52 649.0 0 52 24 42 649.0 0 42 25 44 649.0 0 44 26 39 649.0 0 39 27 43 649.0 0 43 28 53 649.0 0 53 29 38 649.0 0 38 30 56 649.0 0 56 31 42 649.0 0 42 32 40 649.0 0 40 33 58 649.0 0 58 34 44 649.0 0 44 35 41 649.0 0 41 36 47 649.0 0 47 37 57 649.0 0 57 38 52 649.0 0 52 39 65 649.0 0 65 40 71 649.0 0 71 41 86 649.0 0 86 42 73 649.0 0 73 43 93 649.0 0 93 44 76 649.0 0 76 45 55 649.0 0 55 46 68 649.0 0 68 47 945 649.0 0 945 48 461 649.0 0 461 49 883 649.0 0 883 50 123 649.0 0 123 51 68 649.0 0 68 52 56 649.0 0 56 53 66 649.0 0 66 54 54 649.0 0 54 55 60 649.0 0 60 56 63 649.0 0 63 57 62 649.0 0 62 58 64 649.0 0 64 59 239 649.0 0 239 60 118 649.0 0 118 61 296 649.0 0 296 62 183 649.0 0 183 63 77 649.0 0 77 64 68 649.0 0 68 65 59 649.0 0 59 66 55 649.0 0 55 67 41 649.0 0 41 68 44 649.0 0 44 69 55 649.0 0 55 70 55 649.0 0 55 71 57 649.0 0 57 72 67 649.0 0 67 73 67 649.0 0 67 74 58 649.0 0 58 75 55 649.0 0 55 76 65 649.0 0 65 77 182 649.0 0 182 78 110 649.0 0 110 79 147 649.0 0 147 80 52 649.0 0 52 81 72 649.0 0 72 82 85 649.0 0 85 83 154 649.0 0 154 84 355 649.0 0 355 85 324 649.0 0 324 86 445 649.0 0 445 87 51 649.0 0 51 88 68 649.0 0 68 89 80 649.0 0 80 90 67 649.0 0 67 91 140 649.0 0 140 92 111 649.0 0 111 93 88 649.0 0 88 94 66 649.0 0 66 95 59 649.0 0 59 96 43 649.0 0 43 97 43 649.0 0 43 98 43 649.0 0 43 99 47 649.0 0 47 100 40 649.0 0 40 101 33 649.0 0 33 102 55 649.0 0 55 103 36 649.0 0 36 104 32 649.0 0 32 105 28 649.0 0 28 106 44 649.0 0 44 107 53 649.0 0 53 108 39 649.0 0 39 109 46 649.0 0 46 110 43 649.0 0 43 111 55 649.0 0 55 112 216 649.0 0 216 113 303 649.0 0 303 114 208 649.0 0 208 115 64 649.0 0 64 116 60 649.0 0 60 117 86 649.0 0 86 118 79 649.0 0 79 119 59 649.0 0 59 120 50 649.0 0 50 121 46 649.0 0 46 122 41 649.0 0 41 123 44 649.0 0 44 124 49 649.0 0 49 125 41 649.0 0 41 126 53 649.0 0 53 127 178 649.0 0 178 128 121 649.0 0 121 129 210 649.0 0 210 130 183 649.0 0 183 131 154 649.0 0 154 132 235 649.0 0 235 133 275 649.0 0 275 134 285 649.0 0 285 135 188 649.0 0 188 136 238 649.0 0 238 137 108 649.0 0 108 138 146 649.0 0 146 139 174 649.0 0 174 140 463 649.0 0 463 141 5293 649.0 0 5293 142 980 649.0 0 980 143 2837 649.0 0 2837 144 82 649.0 0 82 145 55 649.0 0 55 146 71 649.0 0 71 147 72 649.0 0 72 148 82 649.0 0 82 149 153 649.0 0 153 150 60 649.0 0 60 151 86 649.0 0 86 152 349 649.0 0 349 153 347 649.0 0 347 154 269 649.0 0 269 155 162 649.0 0 162 156 49 649.0 0 49 157 55 649.0 0 55 158 63 649.0 0 63 159 47 649.0 0 47 160 52 649.0 0 52 161 38 649.0 0 38 162 34 649.0 0 34 163 68 649.0 0 68 164 64 649.0 0 64 165 56 649.0 0 56 166 47 649.0 0 47 167 45 649.0 0 45 168 46 649.0 0 46 169 56 649.0 0 56 170 50 649.0 0 50 171 66 649.0 0 66 172 52 649.0 0 52 173 54 649.0 0 54 174 83 649.0 0 83 175 87 649.0 0 87 176 47 649.0 0 47 177 24 649.0 0 24 178 58 649.0 0 58 179 57 649.0 0 57 180 49 649.0 0 49 181 55 649.0 0 55 182 61 649.0 0 61 183 47 649.0 0 47 184 62 649.0 0 62 185 126 649.0 0 126 186 93 649.0 0 93 187 175 649.0 0 175 188 55 649.0 0 55 189 78 649.0 0 78 190 80 649.0 0 80 191 100 649.0 0 100 192 70 649.0 0 70 193 94 649.0 0 94 194 51 649.0 0 51 195 68 649.0 0 68 196 58 649.0 0 58 197 66 649.0 0 66 198 82 649.0 0 82 199 105 649.0 0 105 200 58 649.0 0 58 201 93 649.0 0 93 202 51 649.0 0 51 203 102 649.0 0 102 204 40 649.0 0 40 205 60 649.0 0 60 206 67 649.0 0 67 207 59 649.0 0 59 208 59 649.0 0 59 209 73 649.0 0 73 210 76 649.0 0 76 211 230 649.0 0 230 212 208 649.0 0 208 213 268 649.0 0 268 214 197 649.0 0 197 215 157 649.0 0 157 216 156 649.0 0 156 217 143 649.0 0 143 218 145 649.0 0 145 219 160 649.0 0 160 220 156 649.0 0 156 221 99 649.0 0 99 222 78 649.0 0 78 223 47 649.0 0 47 224 76 649.0 0 76 225 131 649.0 0 131 226 131 649.0 0 131 227 164 649.0 0 164 228 796 649.0 0 796 229 1447 649.0 0 1447 230 571 649.0 0 571 231 193 649.0 0 193 232 273 649.0 0 273 233 109 649.0 0 109 234 110 649.0 0 110 235 587 649.0 0 587 236 427 649.0 0 427 237 698 649.0 0 698 238 501 649.0 0 501 239 459 649.0 0 459 240 293 649.0 0 293 241 117 649.0 0 117 242 67 649.0 0 67 243 135 649.0 0 135 244 146 649.0 0 146 245 267 649.0 0 267 246 327 649.0 0 327 247 396 649.0 0 396 248 333 649.0 0 333 249 223 649.0 0 223 250 282 649.0 0 282 251 166 649.0 0 166 252 354 649.0 0 354 253 218 649.0 0 218 254 270 649.0 0 270 255 599 649.0 0 599 256 882 649.0 0 882 257 1061 649.0 0 1061 258 509 649.0 0 509 259 106 649.0 0 106 260 115 649.0 0 115 261 107 649.0 0 107 262 78 649.0 0 78 263 84 649.0 0 84 264 106 649.0 0 106 265 75 649.0 0 75 266 96 649.0 0 96 267 85 649.0 0 85 268 82 649.0 0 82 269 68 649.0 0 68 270 80 649.0 0 80 271 109 649.0 0 109 272 130 649.0 0 130 273 312 649.0 0 312 274 352 649.0 0 352 275 269 649.0 0 269 276 76 649.0 0 76 277 101 649.0 0 101 278 100 649.0 0 100 279 157 649.0 0 157 280 201 649.0 0 201 281 164 649.0 0 164 282 216 649.0 0 216 283 353 649.0 0 353 284 463 649.0 0 463 285 730 649.0 0 730 286 351 649.0 0 351 287 380 649.0 0 380 288 295 649.0 0 295 289 218 649.0 0 218 290 163 649.0 0 163 291 156 649.0 0 156 292 217 649.0 0 217 293 366 649.0 0 366 294 199 649.0 0 199 295 284 649.0 0 284 296 476 649.0 0 476 297 477 649.0 0 477 298 4456 649.0 0 4456 299 2548 649.0 0 2548 300 6077 649.0 0 6077 301 634 649.0 0 634 Finished in 3.33 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 65,482 Reads with adapters: 16,784 (25.6%) Reads that were too short: 33 (0.1%) Reads written (passing filters): 16,751 (25.6%) Total basepairs processed: 16,137,304 bp Total written (filtered): 4,576,038 bp (28.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16784 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 7 0.0 3 0 0 0 3 4 18 15 0.0 3 0 0 10 3 2 19 259 0.0 3 0 187 26 33 13 20 15946 0.0 4 13960 1424 364 144 54 21 79 0.0 4 17 43 11 3 5 22 220 0.0 4 203 10 4 3 23 87 0.0 4 2 1 0 49 35 25 2 0.0 4 0 0 0 1 1 28 2 0.0 4 0 1 0 0 1 51 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 68 1 0.0 4 1 73 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 117 4 0.0 4 0 0 0 0 4 126 1 0.0 4 0 0 0 0 1 127 114 0.0 4 0 0 0 0 114 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 169 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 218 2 0.0 4 0 0 0 0 2 220 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 1 227 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 3 0.0 4 0 0 0 0 3 236 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.59 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 16,751 Reads with adapters: 1,026 (6.1%) Reads written (passing filters): 16,751 (100.0%) Total basepairs processed: 4,576,038 bp Total written (filtered): 4,563,966 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1026 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.3% C: 16.2% G: 73.4% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 108 261.7 0 108 4 15 65.4 0 15 9 11 0.1 0 0 11 10 63 0.0 1 4 59 11 569 0.0 1 37 532 12 223 0.0 1 11 212 13 25 0.0 1 0 25 16 2 0.0 1 0 2 126 1 0.0 1 1 152 5 0.0 1 4 1 153 1 0.0 1 1 208 1 0.0 1 1 210 2 0.0 1 2