This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.76 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 83,650 (3.1%) Reads that were too short: 20,137 (0.8%) Reads written (passing filters): 63,513 (2.4%) Total basepairs processed: 800,111,879 bp Total written (filtered): 15,919,353 bp (2.0%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 83650 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1955 649.0 0 1955 7 18654 649.0 0 18654 8 467 649.0 0 467 9 17812 649.0 0 17812 10 71 649.0 0 71 11 63 649.0 0 63 12 65 649.0 0 65 13 66 649.0 0 66 14 44 649.0 0 44 15 53 649.0 0 53 16 38 649.0 0 38 17 69 649.0 0 69 18 87 649.0 0 87 19 82 649.0 0 82 20 51 649.0 0 51 21 54 649.0 0 54 22 39 649.0 0 39 23 52 649.0 0 52 24 36 649.0 0 36 25 43 649.0 0 43 26 54 649.0 0 54 27 58 649.0 0 58 28 42 649.0 0 42 29 48 649.0 0 48 30 43 649.0 0 43 31 60 649.0 0 60 32 50 649.0 0 50 33 56 649.0 0 56 34 50 649.0 0 50 35 53 649.0 0 53 36 50 649.0 0 50 37 73 649.0 0 73 38 59 649.0 0 59 39 52 649.0 0 52 40 57 649.0 0 57 41 68 649.0 0 68 42 69 649.0 0 69 43 66 649.0 0 66 44 48 649.0 0 48 45 74 649.0 0 74 46 87 649.0 0 87 47 516 649.0 0 516 48 208 649.0 0 208 49 532 649.0 0 532 50 62 649.0 0 62 51 46 649.0 0 46 52 50 649.0 0 50 53 93 649.0 0 93 54 113 649.0 0 113 55 77 649.0 0 77 56 81 649.0 0 81 57 68 649.0 0 68 58 74 649.0 0 74 59 206 649.0 0 206 60 121 649.0 0 121 61 233 649.0 0 233 62 211 649.0 0 211 63 84 649.0 0 84 64 46 649.0 0 46 65 58 649.0 0 58 66 54 649.0 0 54 67 49 649.0 0 49 68 51 649.0 0 51 69 55 649.0 0 55 70 69 649.0 0 69 71 80 649.0 0 80 72 82 649.0 0 82 73 56 649.0 0 56 74 44 649.0 0 44 75 70 649.0 0 70 76 78 649.0 0 78 77 115 649.0 0 115 78 67 649.0 0 67 79 80 649.0 0 80 80 60 649.0 0 60 81 69 649.0 0 69 82 55 649.0 0 55 83 119 649.0 0 119 84 232 649.0 0 232 85 136 649.0 0 136 86 260 649.0 0 260 87 46 649.0 0 46 88 48 649.0 0 48 89 60 649.0 0 60 90 53 649.0 0 53 91 57 649.0 0 57 92 65 649.0 0 65 93 58 649.0 0 58 94 66 649.0 0 66 95 62 649.0 0 62 96 65 649.0 0 65 97 55 649.0 0 55 98 43 649.0 0 43 99 51 649.0 0 51 100 58 649.0 0 58 101 43 649.0 0 43 102 55 649.0 0 55 103 49 649.0 0 49 104 44 649.0 0 44 105 47 649.0 0 47 106 66 649.0 0 66 107 56 649.0 0 56 108 62 649.0 0 62 109 77 649.0 0 77 110 58 649.0 0 58 111 49 649.0 0 49 112 205 649.0 0 205 113 351 649.0 0 351 114 264 649.0 0 264 115 70 649.0 0 70 116 48 649.0 0 48 117 66 649.0 0 66 118 76 649.0 0 76 119 66 649.0 0 66 120 53 649.0 0 53 121 85 649.0 0 85 122 52 649.0 0 52 123 59 649.0 0 59 124 49 649.0 0 49 125 55 649.0 0 55 126 75 649.0 0 75 127 119 649.0 0 119 128 106 649.0 0 106 129 175 649.0 0 175 130 198 649.0 0 198 131 140 649.0 0 140 132 185 649.0 0 185 133 208 649.0 0 208 134 175 649.0 0 175 135 130 649.0 0 130 136 219 649.0 0 219 137 105 649.0 0 105 138 122 649.0 0 122 139 99 649.0 0 99 140 395 649.0 0 395 141 4395 649.0 0 4395 142 827 649.0 0 827 143 2572 649.0 0 2572 144 68 649.0 0 68 145 40 649.0 0 40 146 77 649.0 0 77 147 104 649.0 0 104 148 93 649.0 0 93 149 117 649.0 0 117 150 52 649.0 0 52 151 76 649.0 0 76 152 211 649.0 0 211 153 233 649.0 0 233 154 210 649.0 0 210 155 139 649.0 0 139 156 56 649.0 0 56 157 50 649.0 0 50 158 72 649.0 0 72 159 66 649.0 0 66 160 54 649.0 0 54 161 60 649.0 0 60 162 54 649.0 0 54 163 64 649.0 0 64 164 72 649.0 0 72 165 63 649.0 0 63 166 65 649.0 0 65 167 57 649.0 0 57 168 58 649.0 0 58 169 62 649.0 0 62 170 57 649.0 0 57 171 78 649.0 0 78 172 72 649.0 0 72 173 74 649.0 0 74 174 82 649.0 0 82 175 107 649.0 0 107 176 62 649.0 0 62 177 43 649.0 0 43 178 46 649.0 0 46 179 45 649.0 0 45 180 50 649.0 0 50 181 57 649.0 0 57 182 48 649.0 0 48 183 62 649.0 0 62 184 75 649.0 0 75 185 153 649.0 0 153 186 125 649.0 0 125 187 232 649.0 0 232 188 68 649.0 0 68 189 67 649.0 0 67 190 43 649.0 0 43 191 62 649.0 0 62 192 56 649.0 0 56 193 72 649.0 0 72 194 46 649.0 0 46 195 62 649.0 0 62 196 57 649.0 0 57 197 49 649.0 0 49 198 61 649.0 0 61 199 58 649.0 0 58 200 51 649.0 0 51 201 105 649.0 0 105 202 55 649.0 0 55 203 149 649.0 0 149 204 37 649.0 0 37 205 53 649.0 0 53 206 36 649.0 0 36 207 61 649.0 0 61 208 61 649.0 0 61 209 81 649.0 0 81 210 73 649.0 0 73 211 180 649.0 0 180 212 127 649.0 0 127 213 145 649.0 0 145 214 77 649.0 0 77 215 73 649.0 0 73 216 131 649.0 0 131 217 73 649.0 0 73 218 80 649.0 0 80 219 55 649.0 0 55 220 70 649.0 0 70 221 49 649.0 0 49 222 38 649.0 0 38 223 36 649.0 0 36 224 73 649.0 0 73 225 110 649.0 0 110 226 100 649.0 0 100 227 105 649.0 0 105 228 727 649.0 0 727 229 1516 649.0 0 1516 230 432 649.0 0 432 231 102 649.0 0 102 232 109 649.0 0 109 233 44 649.0 0 44 234 59 649.0 0 59 235 215 649.0 0 215 236 151 649.0 0 151 237 268 649.0 0 268 238 130 649.0 0 130 239 166 649.0 0 166 240 84 649.0 0 84 241 58 649.0 0 58 242 49 649.0 0 49 243 69 649.0 0 69 244 42 649.0 0 42 245 81 649.0 0 81 246 100 649.0 0 100 247 128 649.0 0 128 248 128 649.0 0 128 249 79 649.0 0 79 250 116 649.0 0 116 251 75 649.0 0 75 252 125 649.0 0 125 253 85 649.0 0 85 254 78 649.0 0 78 255 217 649.0 0 217 256 399 649.0 0 399 257 515 649.0 0 515 258 269 649.0 0 269 259 90 649.0 0 90 260 69 649.0 0 69 261 87 649.0 0 87 262 57 649.0 0 57 263 48 649.0 0 48 264 58 649.0 0 58 265 45 649.0 0 45 266 64 649.0 0 64 267 44 649.0 0 44 268 46 649.0 0 46 269 40 649.0 0 40 270 73 649.0 0 73 271 52 649.0 0 52 272 64 649.0 0 64 273 309 649.0 0 309 274 389 649.0 0 389 275 321 649.0 0 321 276 60 649.0 0 60 277 47 649.0 0 47 278 40 649.0 0 40 279 59 649.0 0 59 280 81 649.0 0 81 281 93 649.0 0 93 282 81 649.0 0 81 283 118 649.0 0 118 284 133 649.0 0 133 285 206 649.0 0 206 286 114 649.0 0 114 287 168 649.0 0 168 288 129 649.0 0 129 289 85 649.0 0 85 290 94 649.0 0 94 291 85 649.0 0 85 292 153 649.0 0 153 293 189 649.0 0 189 294 124 649.0 0 124 295 128 649.0 0 128 296 188 649.0 0 188 297 211 649.0 0 211 298 2008 649.0 0 2008 299 1292 649.0 0 1292 300 3381 649.0 0 3381 301 270 649.0 0 270 Finished in 2.92 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 63,513 Reads with adapters: 18,974 (29.9%) Reads that were too short: 59 (0.1%) Reads written (passing filters): 18,915 (29.8%) Total basepairs processed: 15,919,353 bp Total written (filtered): 5,148,535 bp (32.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18974 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 125 0.0 3 0 120 4 1 17 18189 0.0 3 17773 397 16 3 18 43 0.0 3 6 33 1 3 19 184 0.0 3 171 5 7 1 20 33 0.0 3 1 0 0 32 21 2 0.0 3 0 1 0 1 22 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 39 13 0.0 3 0 0 0 13 42 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 65 0.0 3 0 0 1 64 53 6 0.0 3 0 0 0 6 58 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 82 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 87 5 0.0 3 0 0 0 5 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 101 21 0.0 3 0 0 0 21 102 6 0.0 3 0 0 0 6 119 9 0.0 3 0 0 0 9 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 1 132 3 0.0 3 0 0 0 3 142 2 0.0 3 0 0 0 2 143 197 0.0 3 0 0 4 193 144 1 0.0 3 0 0 0 1 145 6 0.0 3 0 0 0 6 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 1 169 1 0.0 3 0 0 0 1 170 6 0.0 3 0 0 0 6 179 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 217 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 1 278 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.67 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 18,915 Reads with adapters: 1,058 (5.6%) Reads written (passing filters): 18,915 (100.0%) Total basepairs processed: 5,148,535 bp Total written (filtered): 5,145,311 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1058 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.0% C: 7.4% G: 18.4% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1031 295.5 0 1031 4 23 73.9 0 23 9 3 0.1 0 0 3 12 1 0.0 1 0 1