This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.83 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 147,001 (5.5%) Reads that were too short: 43,548 (1.6%) Reads written (passing filters): 103,453 (3.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 28,860,469 bp (3.6%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 147001 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 640 649.0 0 640 7 50826 649.0 0 50826 8 489 649.0 0 489 9 40549 649.0 0 40549 10 70 649.0 0 70 11 10 649.0 0 10 12 13 649.0 0 13 13 7 649.0 0 7 14 15 649.0 0 15 15 9 649.0 0 9 16 12 649.0 0 12 17 10 649.0 0 10 18 7 649.0 0 7 19 5 649.0 0 5 20 12 649.0 0 12 21 9 649.0 0 9 22 6 649.0 0 6 23 9 649.0 0 9 24 7 649.0 0 7 25 6 649.0 0 6 26 15 649.0 0 15 27 9 649.0 0 9 28 7 649.0 0 7 29 27 649.0 0 27 30 15 649.0 0 15 31 8 649.0 0 8 32 13 649.0 0 13 33 16 649.0 0 16 34 13 649.0 0 13 35 13 649.0 0 13 36 24 649.0 0 24 37 31 649.0 0 31 38 9 649.0 0 9 39 10 649.0 0 10 40 10 649.0 0 10 41 8 649.0 0 8 42 13 649.0 0 13 43 9 649.0 0 9 44 5 649.0 0 5 45 14 649.0 0 14 46 20 649.0 0 20 47 6 649.0 0 6 48 8 649.0 0 8 49 12 649.0 0 12 50 11 649.0 0 11 51 11 649.0 0 11 52 15 649.0 0 15 53 22 649.0 0 22 54 31 649.0 0 31 55 23 649.0 0 23 56 21 649.0 0 21 57 13 649.0 0 13 58 11 649.0 0 11 59 16 649.0 0 16 60 12 649.0 0 12 61 18 649.0 0 18 62 17 649.0 0 17 63 14 649.0 0 14 64 17 649.0 0 17 65 14 649.0 0 14 66 14 649.0 0 14 67 17 649.0 0 17 68 9 649.0 0 9 69 16 649.0 0 16 70 22 649.0 0 22 71 81 649.0 0 81 72 25 649.0 0 25 73 28 649.0 0 28 74 154 649.0 0 154 75 141 649.0 0 141 76 80 649.0 0 80 77 12 649.0 0 12 78 26 649.0 0 26 79 44 649.0 0 44 80 81 649.0 0 81 81 66 649.0 0 66 82 174 649.0 0 174 83 162 649.0 0 162 84 154 649.0 0 154 85 26 649.0 0 26 86 32 649.0 0 32 87 19 649.0 0 19 88 28 649.0 0 28 89 42 649.0 0 42 90 18 649.0 0 18 91 12 649.0 0 12 92 24 649.0 0 24 93 26 649.0 0 26 94 27 649.0 0 27 95 30 649.0 0 30 96 29 649.0 0 29 97 31 649.0 0 31 98 18 649.0 0 18 99 28 649.0 0 28 100 27 649.0 0 27 101 22 649.0 0 22 102 28 649.0 0 28 103 25 649.0 0 25 104 31 649.0 0 31 105 26 649.0 0 26 106 28 649.0 0 28 107 33 649.0 0 33 108 19 649.0 0 19 109 18 649.0 0 18 110 36 649.0 0 36 111 150 649.0 0 150 112 29 649.0 0 29 113 23 649.0 0 23 114 24 649.0 0 24 115 25 649.0 0 25 116 20 649.0 0 20 117 29 649.0 0 29 118 34 649.0 0 34 119 35 649.0 0 35 120 32 649.0 0 32 121 49 649.0 0 49 122 64 649.0 0 64 123 246 649.0 0 246 124 52 649.0 0 52 125 90 649.0 0 90 126 80 649.0 0 80 127 49 649.0 0 49 128 60 649.0 0 60 129 28 649.0 0 28 130 41 649.0 0 41 131 32 649.0 0 32 132 35 649.0 0 35 133 29 649.0 0 29 134 27 649.0 0 27 135 37 649.0 0 37 136 36 649.0 0 36 137 50 649.0 0 50 138 46 649.0 0 46 139 39 649.0 0 39 140 49 649.0 0 49 141 30 649.0 0 30 142 119 649.0 0 119 143 35 649.0 0 35 144 39 649.0 0 39 145 45 649.0 0 45 146 57 649.0 0 57 147 45 649.0 0 45 148 45 649.0 0 45 149 43 649.0 0 43 150 40 649.0 0 40 151 46 649.0 0 46 152 48 649.0 0 48 153 62 649.0 0 62 154 50 649.0 0 50 155 47 649.0 0 47 156 52 649.0 0 52 157 57 649.0 0 57 158 48 649.0 0 48 159 48 649.0 0 48 160 44 649.0 0 44 161 43 649.0 0 43 162 54 649.0 0 54 163 59 649.0 0 59 164 47 649.0 0 47 165 84 649.0 0 84 166 103 649.0 0 103 167 1139 649.0 0 1139 168 336 649.0 0 336 169 376 649.0 0 376 170 79 649.0 0 79 171 196 649.0 0 196 172 195 649.0 0 195 173 149 649.0 0 149 174 43 649.0 0 43 175 83 649.0 0 83 176 60 649.0 0 60 177 63 649.0 0 63 178 41 649.0 0 41 179 67 649.0 0 67 180 74 649.0 0 74 181 60 649.0 0 60 182 102 649.0 0 102 183 87 649.0 0 87 184 88 649.0 0 88 185 76 649.0 0 76 186 67 649.0 0 67 187 65 649.0 0 65 188 80 649.0 0 80 189 98 649.0 0 98 190 82 649.0 0 82 191 85 649.0 0 85 192 78 649.0 0 78 193 60 649.0 0 60 194 88 649.0 0 88 195 207 649.0 0 207 196 69 649.0 0 69 197 72 649.0 0 72 198 86 649.0 0 86 199 105 649.0 0 105 200 280 649.0 0 280 201 406 649.0 0 406 202 228 649.0 0 228 203 91 649.0 0 91 204 70 649.0 0 70 205 80 649.0 0 80 206 86 649.0 0 86 207 72 649.0 0 72 208 68 649.0 0 68 209 66 649.0 0 66 210 94 649.0 0 94 211 106 649.0 0 106 212 85 649.0 0 85 213 90 649.0 0 90 214 124 649.0 0 124 215 117 649.0 0 117 216 162 649.0 0 162 217 103 649.0 0 103 218 111 649.0 0 111 219 112 649.0 0 112 220 101 649.0 0 101 221 153 649.0 0 153 222 129 649.0 0 129 223 197 649.0 0 197 224 257 649.0 0 257 225 313 649.0 0 313 226 403 649.0 0 403 227 1262 649.0 0 1262 228 5522 649.0 0 5522 229 14866 649.0 0 14866 230 6366 649.0 0 6366 231 157 649.0 0 157 232 147 649.0 0 147 233 90 649.0 0 90 234 82 649.0 0 82 235 83 649.0 0 83 236 98 649.0 0 98 237 90 649.0 0 90 238 93 649.0 0 93 239 101 649.0 0 101 240 90 649.0 0 90 241 78 649.0 0 78 242 90 649.0 0 90 243 145 649.0 0 145 244 112 649.0 0 112 245 189 649.0 0 189 246 124 649.0 0 124 247 202 649.0 0 202 248 140 649.0 0 140 249 120 649.0 0 120 250 103 649.0 0 103 251 93 649.0 0 93 252 116 649.0 0 116 253 66 649.0 0 66 254 121 649.0 0 121 255 117 649.0 0 117 256 407 649.0 0 407 257 348 649.0 0 348 258 491 649.0 0 491 259 133 649.0 0 133 260 122 649.0 0 122 261 231 649.0 0 231 262 277 649.0 0 277 263 635 649.0 0 635 264 288 649.0 0 288 265 219 649.0 0 219 266 170 649.0 0 170 267 144 649.0 0 144 268 426 649.0 0 426 269 613 649.0 0 613 270 595 649.0 0 595 271 205 649.0 0 205 272 118 649.0 0 118 273 109 649.0 0 109 274 108 649.0 0 108 275 117 649.0 0 117 276 99 649.0 0 99 277 116 649.0 0 116 278 111 649.0 0 111 279 96 649.0 0 96 280 124 649.0 0 124 281 178 649.0 0 178 282 136 649.0 0 136 283 202 649.0 0 202 284 124 649.0 0 124 285 112 649.0 0 112 286 102 649.0 0 102 287 128 649.0 0 128 288 151 649.0 0 151 289 175 649.0 0 175 290 88 649.0 0 88 291 134 649.0 0 134 292 152 649.0 0 152 293 134 649.0 0 134 294 204 649.0 0 204 295 119 649.0 0 119 296 93 649.0 0 93 297 119 649.0 0 119 298 100 649.0 0 100 299 129 649.0 0 129 300 198 649.0 0 198 301 187 649.0 0 187 Finished in 5.00 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 103,453 Reads with adapters: 50,976 (49.3%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 50,944 (49.2%) Total basepairs processed: 28,860,469 bp Total written (filtered): 14,091,608 bp (48.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50976 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 398 0.0 3 0 383 13 2 17 50172 0.0 3 49206 935 28 3 18 105 0.0 3 8 91 5 1 19 33 0.0 3 0 0 30 3 20 62 0.0 3 1 0 0 61 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 139 0.0 3 0 0 0 139 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 1 71 4 0.0 3 0 0 0 4 84 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 2 106 1 0.0 3 0 0 1 113 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 0 5 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 193 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 214 2 0.0 3 0 0 0 2 217 9 0.0 3 0 0 0 9 224 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.80 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 50,944 Reads with adapters: 539 (1.1%) Reads written (passing filters): 50,944 (100.0%) Total basepairs processed: 14,091,608 bp Total written (filtered): 14,089,900 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 539 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.8% C: 19.5% G: 19.9% T: 27.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 468 796.0 0 468 4 61 199.0 0 61 5 4 49.8 0 4 6 3 12.4 0 3 7 2 3.1 0 2 8 1 0.8 0 1