This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.99 s (28 us/read; 2.16 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 1,286,388 (48.4%) Reads that were too short: 49,282 (1.9%) Reads written (passing filters): 1,237,106 (46.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 336,552,650 bp (42.1%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1286388 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1125 649.0 0 1125 7 73355 649.0 0 73355 8 2665 649.0 0 2665 9 63782 649.0 0 63782 10 287 649.0 0 287 11 228 649.0 0 228 12 711 649.0 0 711 13 2048 649.0 0 2048 14 476 649.0 0 476 15 1070 649.0 0 1070 16 504 649.0 0 504 17 618 649.0 0 618 18 1309 649.0 0 1309 19 715 649.0 0 715 20 779 649.0 0 779 21 296 649.0 0 296 22 629 649.0 0 629 23 1543 649.0 0 1543 24 1916 649.0 0 1916 25 3136 649.0 0 3136 26 5940 649.0 0 5940 27 7114 649.0 0 7114 28 15315 649.0 0 15315 29 27236 649.0 0 27236 30 361060 649.0 0 361060 31 379327 649.0 0 379327 32 269900 649.0 0 269900 33 944 649.0 0 944 34 125 649.0 0 125 35 216 649.0 0 216 36 134 649.0 0 134 37 214 649.0 0 214 38 23 649.0 0 23 39 16 649.0 0 16 40 21 649.0 0 21 41 19 649.0 0 19 42 29 649.0 0 29 43 39 649.0 0 39 44 89 649.0 0 89 45 19 649.0 0 19 46 21 649.0 0 21 47 20 649.0 0 20 48 14 649.0 0 14 49 13 649.0 0 13 50 22 649.0 0 22 51 21 649.0 0 21 52 21 649.0 0 21 53 24 649.0 0 24 54 112 649.0 0 112 55 131 649.0 0 131 56 183 649.0 0 183 57 27 649.0 0 27 58 29 649.0 0 29 59 20 649.0 0 20 60 30 649.0 0 30 61 53 649.0 0 53 62 32 649.0 0 32 63 20 649.0 0 20 64 15 649.0 0 15 65 23 649.0 0 23 66 20 649.0 0 20 67 55 649.0 0 55 68 27 649.0 0 27 69 32 649.0 0 32 70 30 649.0 0 30 71 24 649.0 0 24 72 25 649.0 0 25 73 26 649.0 0 26 74 37 649.0 0 37 75 22 649.0 0 22 76 52 649.0 0 52 77 200 649.0 0 200 78 26 649.0 0 26 79 55 649.0 0 55 80 56 649.0 0 56 81 81 649.0 0 81 82 66 649.0 0 66 83 88 649.0 0 88 84 49 649.0 0 49 85 113 649.0 0 113 86 69 649.0 0 69 87 119 649.0 0 119 88 152 649.0 0 152 89 139 649.0 0 139 90 54 649.0 0 54 91 71 649.0 0 71 92 45 649.0 0 45 93 48 649.0 0 48 94 54 649.0 0 54 95 53 649.0 0 53 96 55 649.0 0 55 97 52 649.0 0 52 98 49 649.0 0 49 99 41 649.0 0 41 100 31 649.0 0 31 101 47 649.0 0 47 102 237 649.0 0 237 103 183 649.0 0 183 104 590 649.0 0 590 105 460 649.0 0 460 106 492 649.0 0 492 107 199 649.0 0 199 108 36 649.0 0 36 109 26 649.0 0 26 110 40 649.0 0 40 111 56 649.0 0 56 112 55 649.0 0 55 113 61 649.0 0 61 114 52 649.0 0 52 115 33 649.0 0 33 116 101 649.0 0 101 117 46 649.0 0 46 118 69 649.0 0 69 119 53 649.0 0 53 120 52 649.0 0 52 121 47 649.0 0 47 122 53 649.0 0 53 123 60 649.0 0 60 124 155 649.0 0 155 125 251 649.0 0 251 126 197 649.0 0 197 127 76 649.0 0 76 128 67 649.0 0 67 129 152 649.0 0 152 130 45 649.0 0 45 131 49 649.0 0 49 132 65 649.0 0 65 133 42 649.0 0 42 134 57 649.0 0 57 135 84 649.0 0 84 136 51 649.0 0 51 137 154 649.0 0 154 138 122 649.0 0 122 139 157 649.0 0 157 140 78 649.0 0 78 141 93 649.0 0 93 142 127 649.0 0 127 143 95 649.0 0 95 144 52 649.0 0 52 145 36 649.0 0 36 146 49 649.0 0 49 147 61 649.0 0 61 148 52 649.0 0 52 149 109 649.0 0 109 150 70 649.0 0 70 151 82 649.0 0 82 152 46 649.0 0 46 153 44 649.0 0 44 154 36 649.0 0 36 155 38 649.0 0 38 156 41 649.0 0 41 157 59 649.0 0 59 158 53 649.0 0 53 159 229 649.0 0 229 160 115 649.0 0 115 161 211 649.0 0 211 162 58 649.0 0 58 163 69 649.0 0 69 164 67 649.0 0 67 165 85 649.0 0 85 166 59 649.0 0 59 167 93 649.0 0 93 168 105 649.0 0 105 169 49 649.0 0 49 170 35 649.0 0 35 171 83 649.0 0 83 172 132 649.0 0 132 173 52 649.0 0 52 174 31 649.0 0 31 175 46 649.0 0 46 176 44 649.0 0 44 177 64 649.0 0 64 178 60 649.0 0 60 179 50 649.0 0 50 180 41 649.0 0 41 181 47 649.0 0 47 182 83 649.0 0 83 183 76 649.0 0 76 184 102 649.0 0 102 185 76 649.0 0 76 186 54 649.0 0 54 187 52 649.0 0 52 188 77 649.0 0 77 189 54 649.0 0 54 190 50 649.0 0 50 191 40 649.0 0 40 192 43 649.0 0 43 193 63 649.0 0 63 194 92 649.0 0 92 195 73 649.0 0 73 196 74 649.0 0 74 197 45 649.0 0 45 198 50 649.0 0 50 199 100 649.0 0 100 200 79 649.0 0 79 201 86 649.0 0 86 202 50 649.0 0 50 203 58 649.0 0 58 204 78 649.0 0 78 205 243 649.0 0 243 206 179 649.0 0 179 207 151 649.0 0 151 208 38 649.0 0 38 209 41 649.0 0 41 210 56 649.0 0 56 211 40 649.0 0 40 212 55 649.0 0 55 213 63 649.0 0 63 214 62 649.0 0 62 215 65 649.0 0 65 216 57 649.0 0 57 217 57 649.0 0 57 218 86 649.0 0 86 219 72 649.0 0 72 220 82 649.0 0 82 221 70 649.0 0 70 222 59 649.0 0 59 223 56 649.0 0 56 224 46 649.0 0 46 225 72 649.0 0 72 226 181 649.0 0 181 227 122 649.0 0 122 228 145 649.0 0 145 229 74 649.0 0 74 230 82 649.0 0 82 231 101 649.0 0 101 232 79 649.0 0 79 233 108 649.0 0 108 234 71 649.0 0 71 235 29 649.0 0 29 236 99 649.0 0 99 237 61 649.0 0 61 238 40 649.0 0 40 239 66 649.0 0 66 240 76 649.0 0 76 241 132 649.0 0 132 242 69 649.0 0 69 243 95 649.0 0 95 244 107 649.0 0 107 245 123 649.0 0 123 246 164 649.0 0 164 247 228 649.0 0 228 248 149 649.0 0 149 249 165 649.0 0 165 250 204 649.0 0 204 251 165 649.0 0 165 252 215 649.0 0 215 253 372 649.0 0 372 254 4892 649.0 0 4892 255 1799 649.0 0 1799 256 4187 649.0 0 4187 257 564 649.0 0 564 258 456 649.0 0 456 259 683 649.0 0 683 260 10689 649.0 0 10689 261 7706 649.0 0 7706 262 4440 649.0 0 4440 263 176 649.0 0 176 264 177 649.0 0 177 265 92 649.0 0 92 266 81 649.0 0 81 267 128 649.0 0 128 268 174 649.0 0 174 269 140 649.0 0 140 270 192 649.0 0 192 271 92 649.0 0 92 272 279 649.0 0 279 273 422 649.0 0 422 274 484 649.0 0 484 275 1027 649.0 0 1027 276 987 649.0 0 987 277 1044 649.0 0 1044 278 395 649.0 0 395 279 465 649.0 0 465 280 417 649.0 0 417 281 325 649.0 0 325 282 285 649.0 0 285 283 184 649.0 0 184 284 58 649.0 0 58 285 44 649.0 0 44 286 48 649.0 0 48 287 62 649.0 0 62 288 55 649.0 0 55 289 46 649.0 0 46 290 59 649.0 0 59 291 49 649.0 0 49 292 50 649.0 0 50 293 51 649.0 0 51 294 62 649.0 0 62 295 87 649.0 0 87 296 53 649.0 0 53 297 33 649.0 0 33 298 40 649.0 0 40 299 42 649.0 0 42 300 41 649.0 0 41 301 62 649.0 0 62 Finished in 51.03 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 1,237,106 Reads with adapters: 67,363 (5.4%) Reads that were too short: 252 (0.0%) Reads written (passing filters): 67,111 (5.4%) Total basepairs processed: 336,552,650 bp Total written (filtered): 18,426,822 bp (5.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 67363 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 15 0.0 3 0 0 14 1 16 446 0.0 3 0 439 6 1 17 66098 0.0 3 64817 1248 31 2 18 167 0.0 3 3 152 6 6 19 50 0.0 3 0 0 43 7 20 118 0.0 3 43 3 0 72 23 1 0.0 3 0 1 24 2 0.0 3 0 1 0 1 25 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 1 47 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 6 0.0 3 0 0 1 5 68 5 0.0 3 0 0 0 5 69 5 0.0 3 0 0 0 5 70 2 0.0 3 0 0 0 2 71 3 0.0 3 0 0 1 2 73 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 5 0.0 3 0 0 0 5 77 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 9 0.0 3 0 0 0 9 130 2 0.0 3 0 0 0 2 132 4 0.0 3 0 0 0 4 133 25 0.0 3 0 0 0 25 136 11 0.0 3 0 0 0 11 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 1 141 28 0.0 3 0 0 0 28 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 164 4 0.0 3 0 0 0 4 167 4 0.0 3 1 0 0 3 168 10 0.0 3 0 0 0 10 169 24 0.0 3 0 0 1 23 170 1 0.0 3 0 0 0 1 171 19 0.0 3 0 0 1 18 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 15 0.0 3 0 0 1 14 183 5 0.0 3 0 0 0 5 186 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 0 3 195 2 0.0 3 0 0 0 2 196 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 198 3 0.0 3 0 0 0 3 199 9 0.0 3 0 0 0 9 200 1 0.0 3 0 0 0 1 201 3 0.0 3 0 0 0 3 214 3 0.0 3 0 0 1 2 218 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 221 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 1 247 6 0.0 3 0 0 0 6 248 2 0.0 3 0 1 0 1 254 1 0.0 3 0 0 0 1 255 10 0.0 3 0 0 0 10 256 31 0.0 3 0 0 0 31 257 26 0.0 3 0 0 0 26 258 46 0.0 3 0 0 1 45 259 32 0.0 3 0 0 0 32 260 7 0.0 3 0 0 0 7 261 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 265 5 0.0 3 0 0 0 5 266 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.41 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 67,111 Reads with adapters: 2,888 (4.3%) Reads written (passing filters): 67,111 (100.0%) Total basepairs processed: 18,426,822 bp Total written (filtered): 18,418,064 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2888 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.6% C: 6.6% G: 19.0% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2803 1048.6 0 2803 4 78 262.2 0 78 5 5 65.5 0 5 6 2 16.4 0 2