This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.48 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 176,279 (6.6%) Reads that were too short: 30,078 (1.1%) Reads written (passing filters): 146,201 (5.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 34,143,304 bp (4.3%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 176279 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1890 649.0 0 1890 7 544 649.0 0 544 8 21113 649.0 0 21113 9 18916 649.0 0 18916 10 52 649.0 0 52 11 30 649.0 0 30 12 30 649.0 0 30 13 23 649.0 0 23 14 39 649.0 0 39 15 31 649.0 0 31 16 36 649.0 0 36 17 28 649.0 0 28 18 41 649.0 0 41 19 41 649.0 0 41 20 26 649.0 0 26 21 18 649.0 0 18 22 27 649.0 0 27 23 45 649.0 0 45 24 63 649.0 0 63 25 39 649.0 0 39 26 41 649.0 0 41 27 28 649.0 0 28 28 16 649.0 0 16 29 27 649.0 0 27 30 38 649.0 0 38 31 35 649.0 0 35 32 38 649.0 0 38 33 32 649.0 0 32 34 38 649.0 0 38 35 46 649.0 0 46 36 33 649.0 0 33 37 40 649.0 0 40 38 26 649.0 0 26 39 53 649.0 0 53 40 31 649.0 0 31 41 37 649.0 0 37 42 95 649.0 0 95 43 57 649.0 0 57 44 39 649.0 0 39 45 35 649.0 0 35 46 33 649.0 0 33 47 36 649.0 0 36 48 37 649.0 0 37 49 28 649.0 0 28 50 45 649.0 0 45 51 82 649.0 0 82 52 191 649.0 0 191 53 87 649.0 0 87 54 2405 649.0 0 2405 55 96 649.0 0 96 56 64 649.0 0 64 57 113 649.0 0 113 58 70 649.0 0 70 59 71 649.0 0 71 60 81 649.0 0 81 61 65 649.0 0 65 62 131 649.0 0 131 63 168 649.0 0 168 64 238 649.0 0 238 65 495 649.0 0 495 66 515 649.0 0 515 67 995 649.0 0 995 68 1534 649.0 0 1534 69 20135 649.0 0 20135 70 29841 649.0 0 29841 71 12634 649.0 0 12634 72 80 649.0 0 80 73 34 649.0 0 34 74 36 649.0 0 36 75 91 649.0 0 91 76 99 649.0 0 99 77 211 649.0 0 211 78 60 649.0 0 60 79 71 649.0 0 71 80 333 649.0 0 333 81 281 649.0 0 281 82 188 649.0 0 188 83 75 649.0 0 75 84 61 649.0 0 61 85 33 649.0 0 33 86 61 649.0 0 61 87 85 649.0 0 85 88 61 649.0 0 61 89 74 649.0 0 74 90 160 649.0 0 160 91 96 649.0 0 96 92 75 649.0 0 75 93 111 649.0 0 111 94 69 649.0 0 69 95 94 649.0 0 94 96 345 649.0 0 345 97 151 649.0 0 151 98 88 649.0 0 88 99 138 649.0 0 138 100 736 649.0 0 736 101 777 649.0 0 777 102 2221 649.0 0 2221 103 202 649.0 0 202 104 114 649.0 0 114 105 79 649.0 0 79 106 67 649.0 0 67 107 67 649.0 0 67 108 102 649.0 0 102 109 55 649.0 0 55 110 91 649.0 0 91 111 88 649.0 0 88 112 77 649.0 0 77 113 114 649.0 0 114 114 87 649.0 0 87 115 138 649.0 0 138 116 103 649.0 0 103 117 133 649.0 0 133 118 176 649.0 0 176 119 138 649.0 0 138 120 102 649.0 0 102 121 124 649.0 0 124 122 148 649.0 0 148 123 180 649.0 0 180 124 130 649.0 0 130 125 125 649.0 0 125 126 159 649.0 0 159 127 134 649.0 0 134 128 135 649.0 0 135 129 133 649.0 0 133 130 200 649.0 0 200 131 285 649.0 0 285 132 1538 649.0 0 1538 133 584 649.0 0 584 134 997 649.0 0 997 135 138 649.0 0 138 136 573 649.0 0 573 137 467 649.0 0 467 138 513 649.0 0 513 139 186 649.0 0 186 140 163 649.0 0 163 141 365 649.0 0 365 142 99 649.0 0 99 143 144 649.0 0 144 144 139 649.0 0 139 145 123 649.0 0 123 146 125 649.0 0 125 147 153 649.0 0 153 148 154 649.0 0 154 149 131 649.0 0 131 150 144 649.0 0 144 151 127 649.0 0 127 152 370 649.0 0 370 153 177 649.0 0 177 154 256 649.0 0 256 155 138 649.0 0 138 156 145 649.0 0 145 157 252 649.0 0 252 158 237 649.0 0 237 159 233 649.0 0 233 160 188 649.0 0 188 161 163 649.0 0 163 162 163 649.0 0 163 163 221 649.0 0 221 164 180 649.0 0 180 165 202 649.0 0 202 166 172 649.0 0 172 167 717 649.0 0 717 168 444 649.0 0 444 169 186 649.0 0 186 170 215 649.0 0 215 171 167 649.0 0 167 172 214 649.0 0 214 173 136 649.0 0 136 174 155 649.0 0 155 175 163 649.0 0 163 176 326 649.0 0 326 177 936 649.0 0 936 178 660 649.0 0 660 179 1012 649.0 0 1012 180 399 649.0 0 399 181 252 649.0 0 252 182 529 649.0 0 529 183 494 649.0 0 494 184 702 649.0 0 702 185 331 649.0 0 331 186 282 649.0 0 282 187 230 649.0 0 230 188 172 649.0 0 172 189 188 649.0 0 188 190 215 649.0 0 215 191 171 649.0 0 171 192 291 649.0 0 291 193 132 649.0 0 132 194 177 649.0 0 177 195 175 649.0 0 175 196 171 649.0 0 171 197 395 649.0 0 395 198 282 649.0 0 282 199 294 649.0 0 294 200 161 649.0 0 161 201 179 649.0 0 179 202 709 649.0 0 709 203 755 649.0 0 755 204 822 649.0 0 822 205 641 649.0 0 641 206 151 649.0 0 151 207 133 649.0 0 133 208 131 649.0 0 131 209 174 649.0 0 174 210 199 649.0 0 199 211 256 649.0 0 256 212 238 649.0 0 238 213 241 649.0 0 241 214 162 649.0 0 162 215 157 649.0 0 157 216 195 649.0 0 195 217 276 649.0 0 276 218 205 649.0 0 205 219 217 649.0 0 217 220 147 649.0 0 147 221 147 649.0 0 147 222 358 649.0 0 358 223 571 649.0 0 571 224 595 649.0 0 595 225 446 649.0 0 446 226 198 649.0 0 198 227 197 649.0 0 197 228 309 649.0 0 309 229 230 649.0 0 230 230 149 649.0 0 149 231 116 649.0 0 116 232 129 649.0 0 129 233 168 649.0 0 168 234 154 649.0 0 154 235 119 649.0 0 119 236 138 649.0 0 138 237 176 649.0 0 176 238 231 649.0 0 231 239 232 649.0 0 232 240 179 649.0 0 179 241 199 649.0 0 199 242 481 649.0 0 481 243 579 649.0 0 579 244 707 649.0 0 707 245 432 649.0 0 432 246 179 649.0 0 179 247 155 649.0 0 155 248 136 649.0 0 136 249 142 649.0 0 142 250 203 649.0 0 203 251 865 649.0 0 865 252 366 649.0 0 366 253 664 649.0 0 664 254 179 649.0 0 179 255 343 649.0 0 343 256 334 649.0 0 334 257 303 649.0 0 303 258 227 649.0 0 227 259 256 649.0 0 256 260 225 649.0 0 225 261 196 649.0 0 196 262 202 649.0 0 202 263 249 649.0 0 249 264 263 649.0 0 263 265 466 649.0 0 466 266 328 649.0 0 328 267 253 649.0 0 253 268 176 649.0 0 176 269 295 649.0 0 295 270 325 649.0 0 325 271 553 649.0 0 553 272 394 649.0 0 394 273 404 649.0 0 404 274 347 649.0 0 347 275 289 649.0 0 289 276 408 649.0 0 408 277 459 649.0 0 459 278 483 649.0 0 483 279 340 649.0 0 340 280 194 649.0 0 194 281 201 649.0 0 201 282 181 649.0 0 181 283 253 649.0 0 253 284 357 649.0 0 357 285 320 649.0 0 320 286 273 649.0 0 273 287 236 649.0 0 236 288 205 649.0 0 205 289 161 649.0 0 161 290 198 649.0 0 198 291 377 649.0 0 377 292 558 649.0 0 558 293 344 649.0 0 344 294 414 649.0 0 414 295 192 649.0 0 192 296 177 649.0 0 177 297 198 649.0 0 198 298 222 649.0 0 222 299 286 649.0 0 286 300 300 649.0 0 300 301 375 649.0 0 375 Finished in 6.80 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 146,201 Reads with adapters: 19,994 (13.7%) Reads that were too short: 72 (0.0%) Reads written (passing filters): 19,922 (13.6%) Total basepairs processed: 34,143,304 bp Total written (filtered): 5,392,418 bp (15.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19994 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 7 0.0 3 0 0 5 1 1 19 364 0.0 3 0 335 15 10 4 20 19150 0.0 4 18120 836 139 38 17 21 58 0.0 4 10 39 4 3 2 22 10 0.0 4 2 1 3 1 3 23 71 0.0 4 0 2 0 55 14 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 30 1 0.0 4 0 0 0 0 1 34 4 0.0 4 1 0 0 0 3 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 4 0.0 4 0 0 0 0 4 39 3 0.0 4 0 0 0 0 3 40 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 0 2 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 2 0.0 4 0 0 0 0 2 65 2 0.0 4 0 0 0 0 2 67 2 0.0 4 1 1 68 2 0.0 4 1 0 0 0 1 69 1 0.0 4 1 70 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 76 2 0.0 4 0 0 0 0 2 78 1 0.0 4 1 83 6 0.0 4 0 0 0 0 6 84 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 1 93 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 1 107 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 3 0.0 4 1 1 0 0 1 125 3 0.0 4 0 0 0 0 3 126 3 0.0 4 0 0 0 0 3 127 201 0.0 4 0 0 0 0 201 128 2 0.0 4 0 0 0 1 1 129 1 0.0 4 0 0 0 0 1 130 19 0.0 4 0 0 0 0 19 136 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 1 171 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 194 1 0.0 4 1 195 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 1 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.74 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 19,922 Reads with adapters: 4,709 (23.6%) Reads written (passing filters): 19,922 (100.0%) Total basepairs processed: 5,392,418 bp Total written (filtered): 5,268,739 bp (97.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4709 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 14.3% G: 80.1% T: 3.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 311.3 0 13 4 2 77.8 0 2 5 3 19.5 0 3 6 1 4.9 0 1 8 1 0.3 0 1 9 1105 0.1 0 95 1010 10 2055 0.0 1 157 1898 11 1116 0.0 1 55 1061 12 11 0.0 1 0 11 13 8 0.0 1 0 8 14 1 0.0 1 0 1 18 18 0.0 1 16 2 19 8 0.0 1 5 3 20 2 0.0 1 2 22 1 0.0 1 0 1 58 1 0.0 1 0 1 72 1 0.0 1 1 80 1 0.0 1 0 1 104 1 0.0 1 1 138 4 0.0 1 3 1 150 2 0.0 1 1 1 169 1 0.0 1 1 208 3 0.0 1 1 2 222 1 0.0 1 0 1 223 330 0.0 1 299 31 224 14 0.0 1 13 1 225 2 0.0 1 1 1 226 2 0.0 1 1 1 235 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.