This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.61 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 168,139 (6.3%) Reads that were too short: 19,599 (0.7%) Reads written (passing filters): 148,540 (5.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 35,387,976 bp (4.4%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 168139 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1788 649.0 0 1788 7 448 649.0 0 448 8 20518 649.0 0 20518 9 20903 649.0 0 20903 10 42 649.0 0 42 11 38 649.0 0 38 12 42 649.0 0 42 13 44 649.0 0 44 14 40 649.0 0 40 15 44 649.0 0 44 16 36 649.0 0 36 17 31 649.0 0 31 18 47 649.0 0 47 19 36 649.0 0 36 20 35 649.0 0 35 21 38 649.0 0 38 22 47 649.0 0 47 23 36 649.0 0 36 24 26 649.0 0 26 25 39 649.0 0 39 26 37 649.0 0 37 27 36 649.0 0 36 28 20 649.0 0 20 29 45 649.0 0 45 30 54 649.0 0 54 31 50 649.0 0 50 32 59 649.0 0 59 33 1565 649.0 0 1565 34 114 649.0 0 114 35 57 649.0 0 57 36 51 649.0 0 51 37 44 649.0 0 44 38 34 649.0 0 34 39 60 649.0 0 60 40 26 649.0 0 26 41 52 649.0 0 52 42 125 649.0 0 125 43 49 649.0 0 49 44 44 649.0 0 44 45 49 649.0 0 49 46 51 649.0 0 51 47 33 649.0 0 33 48 33 649.0 0 33 49 39 649.0 0 39 50 63 649.0 0 63 51 83 649.0 0 83 52 164 649.0 0 164 53 91 649.0 0 91 54 152 649.0 0 152 55 56 649.0 0 56 56 85 649.0 0 85 57 110 649.0 0 110 58 98 649.0 0 98 59 74 649.0 0 74 60 73 649.0 0 73 61 86 649.0 0 86 62 141 649.0 0 141 63 165 649.0 0 165 64 240 649.0 0 240 65 472 649.0 0 472 66 530 649.0 0 530 67 1020 649.0 0 1020 68 1254 649.0 0 1254 69 22408 649.0 0 22408 70 34735 649.0 0 34735 71 15510 649.0 0 15510 72 94 649.0 0 94 73 41 649.0 0 41 74 40 649.0 0 40 75 46 649.0 0 46 76 61 649.0 0 61 77 34 649.0 0 34 78 41 649.0 0 41 79 55 649.0 0 55 80 237 649.0 0 237 81 183 649.0 0 183 82 214 649.0 0 214 83 89 649.0 0 89 84 103 649.0 0 103 85 73 649.0 0 73 86 73 649.0 0 73 87 93 649.0 0 93 88 94 649.0 0 94 89 81 649.0 0 81 90 92 649.0 0 92 91 86 649.0 0 86 92 86 649.0 0 86 93 103 649.0 0 103 94 116 649.0 0 116 95 121 649.0 0 121 96 89 649.0 0 89 97 105 649.0 0 105 98 86 649.0 0 86 99 93 649.0 0 93 100 289 649.0 0 289 101 379 649.0 0 379 102 782 649.0 0 782 103 122 649.0 0 122 104 119 649.0 0 119 105 93 649.0 0 93 106 94 649.0 0 94 107 87 649.0 0 87 108 116 649.0 0 116 109 88 649.0 0 88 110 115 649.0 0 115 111 143 649.0 0 143 112 105 649.0 0 105 113 115 649.0 0 115 114 100 649.0 0 100 115 119 649.0 0 119 116 90 649.0 0 90 117 114 649.0 0 114 118 164 649.0 0 164 119 121 649.0 0 121 120 130 649.0 0 130 121 113 649.0 0 113 122 135 649.0 0 135 123 164 649.0 0 164 124 147 649.0 0 147 125 104 649.0 0 104 126 112 649.0 0 112 127 93 649.0 0 93 128 99 649.0 0 99 129 110 649.0 0 110 130 183 649.0 0 183 131 246 649.0 0 246 132 1021 649.0 0 1021 133 476 649.0 0 476 134 765 649.0 0 765 135 138 649.0 0 138 136 339 649.0 0 339 137 322 649.0 0 322 138 405 649.0 0 405 139 161 649.0 0 161 140 159 649.0 0 159 141 381 649.0 0 381 142 117 649.0 0 117 143 119 649.0 0 119 144 140 649.0 0 140 145 125 649.0 0 125 146 91 649.0 0 91 147 99 649.0 0 99 148 114 649.0 0 114 149 131 649.0 0 131 150 131 649.0 0 131 151 117 649.0 0 117 152 297 649.0 0 297 153 164 649.0 0 164 154 216 649.0 0 216 155 86 649.0 0 86 156 117 649.0 0 117 157 189 649.0 0 189 158 192 649.0 0 192 159 180 649.0 0 180 160 165 649.0 0 165 161 173 649.0 0 173 162 114 649.0 0 114 163 193 649.0 0 193 164 179 649.0 0 179 165 213 649.0 0 213 166 172 649.0 0 172 167 790 649.0 0 790 168 475 649.0 0 475 169 171 649.0 0 171 170 138 649.0 0 138 171 139 649.0 0 139 172 161 649.0 0 161 173 124 649.0 0 124 174 135 649.0 0 135 175 135 649.0 0 135 176 259 649.0 0 259 177 538 649.0 0 538 178 460 649.0 0 460 179 549 649.0 0 549 180 327 649.0 0 327 181 171 649.0 0 171 182 255 649.0 0 255 183 211 649.0 0 211 184 399 649.0 0 399 185 223 649.0 0 223 186 208 649.0 0 208 187 192 649.0 0 192 188 157 649.0 0 157 189 183 649.0 0 183 190 166 649.0 0 166 191 150 649.0 0 150 192 255 649.0 0 255 193 112 649.0 0 112 194 138 649.0 0 138 195 133 649.0 0 133 196 144 649.0 0 144 197 219 649.0 0 219 198 149 649.0 0 149 199 198 649.0 0 198 200 126 649.0 0 126 201 114 649.0 0 114 202 283 649.0 0 283 203 315 649.0 0 315 204 362 649.0 0 362 205 237 649.0 0 237 206 129 649.0 0 129 207 133 649.0 0 133 208 108 649.0 0 108 209 115 649.0 0 115 210 139 649.0 0 139 211 187 649.0 0 187 212 170 649.0 0 170 213 159 649.0 0 159 214 126 649.0 0 126 215 156 649.0 0 156 216 142 649.0 0 142 217 182 649.0 0 182 218 147 649.0 0 147 219 158 649.0 0 158 220 102 649.0 0 102 221 107 649.0 0 107 222 163 649.0 0 163 223 304 649.0 0 304 224 360 649.0 0 360 225 302 649.0 0 302 226 194 649.0 0 194 227 204 649.0 0 204 228 266 649.0 0 266 229 211 649.0 0 211 230 123 649.0 0 123 231 155 649.0 0 155 232 142 649.0 0 142 233 143 649.0 0 143 234 175 649.0 0 175 235 133 649.0 0 133 236 116 649.0 0 116 237 136 649.0 0 136 238 129 649.0 0 129 239 147 649.0 0 147 240 135 649.0 0 135 241 147 649.0 0 147 242 226 649.0 0 226 243 260 649.0 0 260 244 360 649.0 0 360 245 234 649.0 0 234 246 161 649.0 0 161 247 135 649.0 0 135 248 121 649.0 0 121 249 141 649.0 0 141 250 183 649.0 0 183 251 411 649.0 0 411 252 230 649.0 0 230 253 297 649.0 0 297 254 146 649.0 0 146 255 154 649.0 0 154 256 227 649.0 0 227 257 181 649.0 0 181 258 145 649.0 0 145 259 170 649.0 0 170 260 200 649.0 0 200 261 141 649.0 0 141 262 161 649.0 0 161 263 203 649.0 0 203 264 206 649.0 0 206 265 281 649.0 0 281 266 216 649.0 0 216 267 158 649.0 0 158 268 136 649.0 0 136 269 200 649.0 0 200 270 249 649.0 0 249 271 364 649.0 0 364 272 208 649.0 0 208 273 233 649.0 0 233 274 234 649.0 0 234 275 185 649.0 0 185 276 238 649.0 0 238 277 254 649.0 0 254 278 229 649.0 0 229 279 226 649.0 0 226 280 124 649.0 0 124 281 173 649.0 0 173 282 165 649.0 0 165 283 185 649.0 0 185 284 210 649.0 0 210 285 190 649.0 0 190 286 224 649.0 0 224 287 173 649.0 0 173 288 170 649.0 0 170 289 137 649.0 0 137 290 161 649.0 0 161 291 212 649.0 0 212 292 334 649.0 0 334 293 236 649.0 0 236 294 270 649.0 0 270 295 165 649.0 0 165 296 149 649.0 0 149 297 156 649.0 0 156 298 191 649.0 0 191 299 245 649.0 0 245 300 265 649.0 0 265 301 218 649.0 0 218 Finished in 6.26 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 148,540 Reads with adapters: 21,988 (14.8%) Reads that were too short: 56 (0.0%) Reads written (passing filters): 21,932 (14.8%) Total basepairs processed: 35,387,976 bp Total written (filtered): 6,030,716 bp (17.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21988 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 27 0.0 3 0 0 3 24 16 161 0.0 3 0 160 1 17 20037 0.0 3 19620 394 22 1 18 61 0.0 3 15 43 3 19 28 0.0 3 6 7 12 3 20 1506 0.0 3 1455 21 1 29 21 4 0.0 3 0 4 24 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 5 0.0 3 0 0 1 4 34 4 0.0 3 0 0 0 4 37 8 0.0 3 0 0 0 8 40 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 52 3 0.0 3 0 0 0 3 55 16 0.0 3 0 0 1 15 56 1 0.0 3 1 57 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 1 1 60 18 0.0 3 0 0 0 18 65 2 0.0 3 1 0 0 1 66 1 0.0 3 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 17 0.0 3 0 0 1 16 92 2 0.0 3 0 0 0 2 94 3 0.0 3 0 0 0 3 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 124 7 0.0 3 0 0 0 7 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 1 150 5 0.0 3 0 0 0 5 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 182 3 0.0 3 0 0 0 3 190 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 7 0.0 3 0 0 0 7 220 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.78 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 21,932 Reads with adapters: 331 (1.5%) Reads written (passing filters): 21,932 (100.0%) Total basepairs processed: 6,030,716 bp Total written (filtered): 6,029,651 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 331 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.0% C: 7.9% G: 33.8% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 262 342.7 0 262 4 67 85.7 0 67 5 1 21.4 0 1 6 1 5.4 0 1