This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.61 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 169,456 (6.4%) Reads that were too short: 11,918 (0.4%) Reads written (passing filters): 157,538 (5.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 43,596,884 bp (5.4%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 169456 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2084 649.0 0 2084 7 61073 649.0 0 61073 8 2677 649.0 0 2677 9 65282 649.0 0 65282 10 174 649.0 0 174 11 56 649.0 0 56 12 54 649.0 0 54 13 63 649.0 0 63 14 46 649.0 0 46 15 47 649.0 0 47 16 35 649.0 0 35 17 56 649.0 0 56 18 62 649.0 0 62 19 48 649.0 0 48 20 69 649.0 0 69 21 70 649.0 0 70 22 204 649.0 0 204 23 646 649.0 0 646 24 391 649.0 0 391 25 384 649.0 0 384 26 55 649.0 0 55 27 45 649.0 0 45 28 42 649.0 0 42 29 58 649.0 0 58 30 55 649.0 0 55 31 55 649.0 0 55 32 44 649.0 0 44 33 65 649.0 0 65 34 101 649.0 0 101 35 81 649.0 0 81 36 75 649.0 0 75 37 72 649.0 0 72 38 48 649.0 0 48 39 52 649.0 0 52 40 58 649.0 0 58 41 60 649.0 0 60 42 48 649.0 0 48 43 36 649.0 0 36 44 61 649.0 0 61 45 43 649.0 0 43 46 55 649.0 0 55 47 44 649.0 0 44 48 42 649.0 0 42 49 42 649.0 0 42 50 63 649.0 0 63 51 54 649.0 0 54 52 57 649.0 0 57 53 46 649.0 0 46 54 47 649.0 0 47 55 53 649.0 0 53 56 54 649.0 0 54 57 48 649.0 0 48 58 44 649.0 0 44 59 51 649.0 0 51 60 41 649.0 0 41 61 47 649.0 0 47 62 39 649.0 0 39 63 34 649.0 0 34 64 62 649.0 0 62 65 57 649.0 0 57 66 60 649.0 0 60 67 46 649.0 0 46 68 88 649.0 0 88 69 74 649.0 0 74 70 96 649.0 0 96 71 82 649.0 0 82 72 84 649.0 0 84 73 379 649.0 0 379 74 451 649.0 0 451 75 81 649.0 0 81 76 42 649.0 0 42 77 60 649.0 0 60 78 52 649.0 0 52 79 46 649.0 0 46 80 152 649.0 0 152 81 129 649.0 0 129 82 145 649.0 0 145 83 104 649.0 0 104 84 86 649.0 0 86 85 108 649.0 0 108 86 187 649.0 0 187 87 131 649.0 0 131 88 576 649.0 0 576 89 356 649.0 0 356 90 1172 649.0 0 1172 91 46 649.0 0 46 92 145 649.0 0 145 93 42 649.0 0 42 94 60 649.0 0 60 95 70 649.0 0 70 96 240 649.0 0 240 97 201 649.0 0 201 98 119 649.0 0 119 99 104 649.0 0 104 100 201 649.0 0 201 101 1253 649.0 0 1253 102 857 649.0 0 857 103 652 649.0 0 652 104 95 649.0 0 95 105 50 649.0 0 50 106 80 649.0 0 80 107 40 649.0 0 40 108 43 649.0 0 43 109 115 649.0 0 115 110 80 649.0 0 80 111 91 649.0 0 91 112 72 649.0 0 72 113 157 649.0 0 157 114 184 649.0 0 184 115 62 649.0 0 62 116 67 649.0 0 67 117 94 649.0 0 94 118 74 649.0 0 74 119 71 649.0 0 71 120 164 649.0 0 164 121 67 649.0 0 67 122 79 649.0 0 79 123 56 649.0 0 56 124 102 649.0 0 102 125 129 649.0 0 129 126 387 649.0 0 387 127 4211 649.0 0 4211 128 527 649.0 0 527 129 418 649.0 0 418 130 108 649.0 0 108 131 86 649.0 0 86 132 58 649.0 0 58 133 74 649.0 0 74 134 45 649.0 0 45 135 57 649.0 0 57 136 65 649.0 0 65 137 42 649.0 0 42 138 47 649.0 0 47 139 56 649.0 0 56 140 75 649.0 0 75 141 189 649.0 0 189 142 92 649.0 0 92 143 122 649.0 0 122 144 906 649.0 0 906 145 227 649.0 0 227 146 100 649.0 0 100 147 60 649.0 0 60 148 54 649.0 0 54 149 54 649.0 0 54 150 55 649.0 0 55 151 74 649.0 0 74 152 57 649.0 0 57 153 57 649.0 0 57 154 45 649.0 0 45 155 35 649.0 0 35 156 48 649.0 0 48 157 53 649.0 0 53 158 54 649.0 0 54 159 36 649.0 0 36 160 52 649.0 0 52 161 56 649.0 0 56 162 38 649.0 0 38 163 45 649.0 0 45 164 31 649.0 0 31 165 35 649.0 0 35 166 35 649.0 0 35 167 48 649.0 0 48 168 54 649.0 0 54 169 56 649.0 0 56 170 49 649.0 0 49 171 45 649.0 0 45 172 49 649.0 0 49 173 49 649.0 0 49 174 55 649.0 0 55 175 65 649.0 0 65 176 138 649.0 0 138 177 138 649.0 0 138 178 83 649.0 0 83 179 44 649.0 0 44 180 45 649.0 0 45 181 31 649.0 0 31 182 54 649.0 0 54 183 61 649.0 0 61 184 27 649.0 0 27 185 37 649.0 0 37 186 36 649.0 0 36 187 71 649.0 0 71 188 47 649.0 0 47 189 52 649.0 0 52 190 46 649.0 0 46 191 47 649.0 0 47 192 62 649.0 0 62 193 147 649.0 0 147 194 71 649.0 0 71 195 88 649.0 0 88 196 103 649.0 0 103 197 53 649.0 0 53 198 46 649.0 0 46 199 51 649.0 0 51 200 68 649.0 0 68 201 152 649.0 0 152 202 135 649.0 0 135 203 92 649.0 0 92 204 71 649.0 0 71 205 49 649.0 0 49 206 59 649.0 0 59 207 67 649.0 0 67 208 104 649.0 0 104 209 84 649.0 0 84 210 64 649.0 0 64 211 47 649.0 0 47 212 51 649.0 0 51 213 57 649.0 0 57 214 82 649.0 0 82 215 55 649.0 0 55 216 75 649.0 0 75 217 56 649.0 0 56 218 88 649.0 0 88 219 169 649.0 0 169 220 54 649.0 0 54 221 94 649.0 0 94 222 70 649.0 0 70 223 204 649.0 0 204 224 90 649.0 0 90 225 103 649.0 0 103 226 73 649.0 0 73 227 61 649.0 0 61 228 37 649.0 0 37 229 64 649.0 0 64 230 53 649.0 0 53 231 79 649.0 0 79 232 68 649.0 0 68 233 62 649.0 0 62 234 79 649.0 0 79 235 90 649.0 0 90 236 97 649.0 0 97 237 50 649.0 0 50 238 84 649.0 0 84 239 145 649.0 0 145 240 146 649.0 0 146 241 145 649.0 0 145 242 91 649.0 0 91 243 110 649.0 0 110 244 69 649.0 0 69 245 73 649.0 0 73 246 63 649.0 0 63 247 72 649.0 0 72 248 57 649.0 0 57 249 81 649.0 0 81 250 55 649.0 0 55 251 47 649.0 0 47 252 62 649.0 0 62 253 68 649.0 0 68 254 88 649.0 0 88 255 95 649.0 0 95 256 114 649.0 0 114 257 106 649.0 0 106 258 126 649.0 0 126 259 979 649.0 0 979 260 1277 649.0 0 1277 261 1674 649.0 0 1674 262 354 649.0 0 354 263 118 649.0 0 118 264 44 649.0 0 44 265 39 649.0 0 39 266 36 649.0 0 36 267 53 649.0 0 53 268 75 649.0 0 75 269 50 649.0 0 50 270 49 649.0 0 49 271 29 649.0 0 29 272 40 649.0 0 40 273 50 649.0 0 50 274 58 649.0 0 58 275 48 649.0 0 48 276 45 649.0 0 45 277 41 649.0 0 41 278 69 649.0 0 69 279 253 649.0 0 253 280 136 649.0 0 136 281 311 649.0 0 311 282 55 649.0 0 55 283 99 649.0 0 99 284 41 649.0 0 41 285 29 649.0 0 29 286 138 649.0 0 138 287 51 649.0 0 51 288 63 649.0 0 63 289 43 649.0 0 43 290 56 649.0 0 56 291 118 649.0 0 118 292 83 649.0 0 83 293 80 649.0 0 80 294 70 649.0 0 70 295 91 649.0 0 91 296 70 649.0 0 70 297 64 649.0 0 64 298 48 649.0 0 48 299 52 649.0 0 52 300 52 649.0 0 52 301 57 649.0 0 57 Finished in 8.10 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 157,538 Reads with adapters: 62,587 (39.7%) Reads that were too short: 63 (0.0%) Reads written (passing filters): 62,524 (39.7%) Total basepairs processed: 43,596,884 bp Total written (filtered): 17,069,196 bp (39.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 62587 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 21 0.0 3 0 0 16 3 2 19 640 0.0 3 0 565 48 22 5 20 60457 0.0 4 57330 2575 413 108 31 21 145 0.0 4 17 115 8 3 2 22 30 0.0 4 7 5 15 2 1 23 647 0.0 4 512 26 4 79 26 24 9 0.0 4 0 3 0 2 4 26 5 0.0 4 0 0 0 4 1 27 1 0.0 4 0 0 0 1 32 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 39 2 0.0 4 2 41 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 77 3 0.0 4 0 0 0 0 3 86 1 0.0 4 0 0 0 0 1 92 3 0.0 4 0 0 0 0 3 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 1 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 537 0.0 4 0 0 0 0 537 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 141 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 1 178 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 1 198 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 2 0.0 4 0 0 0 0 2 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 216 1 0.0 4 1 222 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 15 0.0 4 0 0 0 1 14 229 11 0.0 4 0 0 0 1 10 230 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 238 2 0.0 4 0 0 0 0 2 257 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.17 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 62,524 Reads with adapters: 3,966 (6.3%) Reads written (passing filters): 62,524 (100.0%) Total basepairs processed: 17,069,196 bp Total written (filtered): 17,017,428 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3966 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.5% C: 13.2% G: 77.8% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 233 976.9 0 233 4 23 244.2 0 23 5 7 61.1 0 7 7 14 3.8 0 14 9 34 0.2 0 2 32 10 131 0.1 1 6 125 11 2852 0.0 1 131 2721 12 534 0.0 1 10 524 13 80 0.0 1 2 78 15 4 0.0 1 0 4 16 2 0.0 1 0 2 77 1 0.0 1 1 152 7 0.0 1 7 180 1 0.0 1 1 191 2 0.0 1 0 2 204 1 0.0 1 1 208 2 0.0 1 1 1 209 1 0.0 1 1 210 34 0.0 1 29 5 211 2 0.0 1 2 222 1 0.0 1 1