This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.47 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 101,043 (3.8%) Reads that were too short: 14,517 (0.5%) Reads written (passing filters): 86,526 (3.3%) Total basepairs processed: 800,111,879 bp Total written (filtered): 23,405,657 bp (2.9%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 101043 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 507 649.0 0 507 7 1514 649.0 0 1514 8 50796 649.0 0 50796 9 15044 649.0 0 15044 10 58 649.0 0 58 11 92 649.0 0 92 12 7 649.0 0 7 13 24 649.0 0 24 14 16 649.0 0 16 15 41 649.0 0 41 16 28 649.0 0 28 17 43 649.0 0 43 18 51 649.0 0 51 19 56 649.0 0 56 20 26 649.0 0 26 21 17 649.0 0 17 22 18 649.0 0 18 23 18 649.0 0 18 24 22 649.0 0 22 25 28 649.0 0 28 26 22 649.0 0 22 27 25 649.0 0 25 28 162 649.0 0 162 29 146 649.0 0 146 30 151 649.0 0 151 31 28 649.0 0 28 32 21 649.0 0 21 33 22 649.0 0 22 34 21 649.0 0 21 35 21 649.0 0 21 36 28 649.0 0 28 37 26 649.0 0 26 38 22 649.0 0 22 39 19 649.0 0 19 40 28 649.0 0 28 41 22 649.0 0 22 42 35 649.0 0 35 43 55 649.0 0 55 44 105 649.0 0 105 45 590 649.0 0 590 46 162 649.0 0 162 47 268 649.0 0 268 48 14 649.0 0 14 49 19 649.0 0 19 50 26 649.0 0 26 51 21 649.0 0 21 52 15 649.0 0 15 53 17 649.0 0 17 54 19 649.0 0 19 55 12 649.0 0 12 56 19 649.0 0 19 57 18 649.0 0 18 58 13 649.0 0 13 59 12 649.0 0 12 60 15 649.0 0 15 61 20 649.0 0 20 62 18 649.0 0 18 63 21 649.0 0 21 64 19 649.0 0 19 65 16 649.0 0 16 66 22 649.0 0 22 67 17 649.0 0 17 68 20 649.0 0 20 69 39 649.0 0 39 70 69 649.0 0 69 71 152 649.0 0 152 72 106 649.0 0 106 73 160 649.0 0 160 74 98 649.0 0 98 75 51 649.0 0 51 76 143 649.0 0 143 77 35 649.0 0 35 78 54 649.0 0 54 79 71 649.0 0 71 80 90 649.0 0 90 81 90 649.0 0 90 82 451 649.0 0 451 83 172 649.0 0 172 84 189 649.0 0 189 85 292 649.0 0 292 86 165 649.0 0 165 87 32 649.0 0 32 88 59 649.0 0 59 89 68 649.0 0 68 90 36 649.0 0 36 91 57 649.0 0 57 92 66 649.0 0 66 93 90 649.0 0 90 94 1099 649.0 0 1099 95 584 649.0 0 584 96 1103 649.0 0 1103 97 259 649.0 0 259 98 37 649.0 0 37 99 20 649.0 0 20 100 36 649.0 0 36 101 43 649.0 0 43 102 18 649.0 0 18 103 36 649.0 0 36 104 38 649.0 0 38 105 24 649.0 0 24 106 41 649.0 0 41 107 35 649.0 0 35 108 28 649.0 0 28 109 35 649.0 0 35 110 31 649.0 0 31 111 52 649.0 0 52 112 62 649.0 0 62 113 385 649.0 0 385 114 535 649.0 0 535 115 433 649.0 0 433 116 270 649.0 0 270 117 60 649.0 0 60 118 47 649.0 0 47 119 48 649.0 0 48 120 49 649.0 0 49 121 46 649.0 0 46 122 71 649.0 0 71 123 65 649.0 0 65 124 60 649.0 0 60 125 61 649.0 0 61 126 44 649.0 0 44 127 52 649.0 0 52 128 75 649.0 0 75 129 71 649.0 0 71 130 65 649.0 0 65 131 69 649.0 0 69 132 84 649.0 0 84 133 114 649.0 0 114 134 136 649.0 0 136 135 411 649.0 0 411 136 478 649.0 0 478 137 265 649.0 0 265 138 360 649.0 0 360 139 227 649.0 0 227 140 159 649.0 0 159 141 89 649.0 0 89 142 84 649.0 0 84 143 75 649.0 0 75 144 79 649.0 0 79 145 57 649.0 0 57 146 54 649.0 0 54 147 40 649.0 0 40 148 47 649.0 0 47 149 55 649.0 0 55 150 43 649.0 0 43 151 37 649.0 0 37 152 59 649.0 0 59 153 42 649.0 0 42 154 49 649.0 0 49 155 72 649.0 0 72 156 50 649.0 0 50 157 80 649.0 0 80 158 45 649.0 0 45 159 42 649.0 0 42 160 20 649.0 0 20 161 31 649.0 0 31 162 102 649.0 0 102 163 110 649.0 0 110 164 106 649.0 0 106 165 73 649.0 0 73 166 55 649.0 0 55 167 140 649.0 0 140 168 171 649.0 0 171 169 136 649.0 0 136 170 108 649.0 0 108 171 48 649.0 0 48 172 62 649.0 0 62 173 47 649.0 0 47 174 38 649.0 0 38 175 55 649.0 0 55 176 36 649.0 0 36 177 40 649.0 0 40 178 66 649.0 0 66 179 135 649.0 0 135 180 67 649.0 0 67 181 142 649.0 0 142 182 41 649.0 0 41 183 67 649.0 0 67 184 182 649.0 0 182 185 240 649.0 0 240 186 176 649.0 0 176 187 124 649.0 0 124 188 75 649.0 0 75 189 57 649.0 0 57 190 47 649.0 0 47 191 44 649.0 0 44 192 47 649.0 0 47 193 43 649.0 0 43 194 45 649.0 0 45 195 43 649.0 0 43 196 35 649.0 0 35 197 40 649.0 0 40 198 66 649.0 0 66 199 158 649.0 0 158 200 88 649.0 0 88 201 107 649.0 0 107 202 140 649.0 0 140 203 105 649.0 0 105 204 270 649.0 0 270 205 229 649.0 0 229 206 283 649.0 0 283 207 188 649.0 0 188 208 73 649.0 0 73 209 111 649.0 0 111 210 101 649.0 0 101 211 94 649.0 0 94 212 77 649.0 0 77 213 59 649.0 0 59 214 47 649.0 0 47 215 71 649.0 0 71 216 86 649.0 0 86 217 64 649.0 0 64 218 57 649.0 0 57 219 60 649.0 0 60 220 86 649.0 0 86 221 83 649.0 0 83 222 133 649.0 0 133 223 148 649.0 0 148 224 1043 649.0 0 1043 225 1753 649.0 0 1753 226 1934 649.0 0 1934 227 1269 649.0 0 1269 228 478 649.0 0 478 229 58 649.0 0 58 230 39 649.0 0 39 231 64 649.0 0 64 232 58 649.0 0 58 233 51 649.0 0 51 234 114 649.0 0 114 235 96 649.0 0 96 236 121 649.0 0 121 237 75 649.0 0 75 238 59 649.0 0 59 239 207 649.0 0 207 240 103 649.0 0 103 241 199 649.0 0 199 242 45 649.0 0 45 243 51 649.0 0 51 244 44 649.0 0 44 245 59 649.0 0 59 246 43 649.0 0 43 247 36 649.0 0 36 248 67 649.0 0 67 249 40 649.0 0 40 250 33 649.0 0 33 251 18 649.0 0 18 252 31 649.0 0 31 253 81 649.0 0 81 254 78 649.0 0 78 255 100 649.0 0 100 256 29 649.0 0 29 257 99 649.0 0 99 258 67 649.0 0 67 259 184 649.0 0 184 260 67 649.0 0 67 261 98 649.0 0 98 262 51 649.0 0 51 263 70 649.0 0 70 264 134 649.0 0 134 265 117 649.0 0 117 266 189 649.0 0 189 267 35 649.0 0 35 268 48 649.0 0 48 269 62 649.0 0 62 270 49 649.0 0 49 271 51 649.0 0 51 272 58 649.0 0 58 273 59 649.0 0 59 274 84 649.0 0 84 275 72 649.0 0 72 276 44 649.0 0 44 277 32 649.0 0 32 278 47 649.0 0 47 279 49 649.0 0 49 280 138 649.0 0 138 281 67 649.0 0 67 282 110 649.0 0 110 283 35 649.0 0 35 284 23 649.0 0 23 285 37 649.0 0 37 286 61 649.0 0 61 287 40 649.0 0 40 288 35 649.0 0 35 289 49 649.0 0 49 290 44 649.0 0 44 291 53 649.0 0 53 292 64 649.0 0 64 293 63 649.0 0 63 294 84 649.0 0 84 295 113 649.0 0 113 296 82 649.0 0 82 297 103 649.0 0 103 298 82 649.0 0 82 299 141 649.0 0 141 300 56 649.0 0 56 301 230 649.0 0 230 Finished in 4.52 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 86,526 Reads with adapters: 16,616 (19.2%) Reads that were too short: 89 (0.1%) Reads written (passing filters): 16,527 (19.1%) Total basepairs processed: 23,405,657 bp Total written (filtered): 4,476,945 bp (19.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16616 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 2 18 11 0.0 3 0 0 6 2 3 19 181 0.0 3 0 164 6 6 5 20 16034 0.0 4 15255 632 107 25 15 21 42 0.0 4 16 23 1 0 2 22 9 0.0 4 2 0 5 1 1 23 45 0.0 4 0 0 1 31 13 26 1 0.0 4 1 27 24 0.0 4 0 0 0 0 24 36 2 0.0 4 0 0 0 0 2 37 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 44 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 89 19 0.0 4 0 0 0 0 19 90 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 3 0.0 4 1 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 86 0.0 4 0 0 0 0 86 129 1 0.0 4 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 1 148 4 0.0 4 0 0 0 1 3 149 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 1 155 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 1 162 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 2 0.0 4 0 0 0 0 2 172 27 0.0 4 0 0 0 0 27 176 1 0.0 4 0 1 178 1 0.0 4 0 0 0 0 1 180 3 0.0 4 0 0 0 0 3 181 1 0.0 4 0 1 183 2 0.0 4 1 0 0 0 1 186 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 1 197 1 0.0 4 0 0 0 1 198 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 3 0.0 4 0 0 0 1 2 210 1 0.0 4 0 1 211 1 0.0 4 0 0 1 219 7 0.0 4 3 2 1 0 1 220 4 0.0 4 2 0 1 0 1 221 3 0.0 4 0 1 0 1 1 225 2 0.0 4 1 0 0 0 1 226 3 0.0 4 1 1 0 0 1 227 1 0.0 4 1 228 5 0.0 4 1 0 0 0 4 233 2 0.0 4 0 1 0 0 1 234 2 0.0 4 0 0 0 1 1 235 3 0.0 4 0 0 0 0 3 243 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 250 3 0.0 4 0 1 0 0 2 256 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 0 3 265 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 1 277 1 0.0 4 0 0 1 280 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 3 0.0 4 0 0 0 0 3 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 16,527 Reads with adapters: 3,841 (23.2%) Reads written (passing filters): 16,527 (100.0%) Total basepairs processed: 4,476,945 bp Total written (filtered): 4,433,112 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3841 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 8.7% G: 86.7% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 258.2 0 3 4 2 64.6 0 2 7 1 1.0 0 1 8 3 0.3 0 3 9 617 0.1 0 50 567 10 2742 0.0 1 207 2535 11 380 0.0 1 15 365 12 16 0.0 1 0 16 13 21 0.0 1 5 16 14 1 0.0 1 0 1 18 6 0.0 1 5 1 19 2 0.0 1 1 1 21 1 0.0 1 1 22 1 0.0 1 1 33 1 0.0 1 1 60 1 0.0 1 0 1 71 1 0.0 1 1 80 7 0.0 1 3 4 90 2 0.0 1 1 1 114 4 0.0 1 4 115 1 0.0 1 1 121 1 0.0 1 0 1 122 9 0.0 1 6 3 123 1 0.0 1 1 145 3 0.0 1 3 150 3 0.0 1 2 1 151 1 0.0 1 0 1 156 1 0.0 1 1 189 1 0.0 1 0 1 203 1 0.0 1 0 1 208 3 0.0 1 2 1 223 3 0.0 1 2 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.