This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.62 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 94,158 (3.5%) Reads that were too short: 10,477 (0.4%) Reads written (passing filters): 83,681 (3.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 22,983,782 bp (2.9%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 94158 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 329 649.0 0 329 7 865 649.0 0 865 8 50789 649.0 0 50789 9 17502 649.0 0 17502 10 29 649.0 0 29 11 15 649.0 0 15 12 10 649.0 0 10 13 12 649.0 0 12 14 10 649.0 0 10 15 14 649.0 0 14 16 15 649.0 0 15 17 18 649.0 0 18 18 27 649.0 0 27 19 23 649.0 0 23 20 19 649.0 0 19 21 9 649.0 0 9 22 7 649.0 0 7 23 15 649.0 0 15 24 17 649.0 0 17 25 5 649.0 0 5 26 9 649.0 0 9 27 8 649.0 0 8 28 18 649.0 0 18 29 32 649.0 0 32 30 50 649.0 0 50 31 17 649.0 0 17 32 16 649.0 0 16 33 12 649.0 0 12 34 15 649.0 0 15 35 22 649.0 0 22 36 14 649.0 0 14 37 24 649.0 0 24 38 11 649.0 0 11 39 17 649.0 0 17 40 5 649.0 0 5 41 12 649.0 0 12 42 28 649.0 0 28 43 48 649.0 0 48 44 92 649.0 0 92 45 312 649.0 0 312 46 141 649.0 0 141 47 225 649.0 0 225 48 14 649.0 0 14 49 8 649.0 0 8 50 17 649.0 0 17 51 13 649.0 0 13 52 11 649.0 0 11 53 10 649.0 0 10 54 14 649.0 0 14 55 12 649.0 0 12 56 13 649.0 0 13 57 10 649.0 0 10 58 19 649.0 0 19 59 12 649.0 0 12 60 15 649.0 0 15 61 14 649.0 0 14 62 13 649.0 0 13 63 11 649.0 0 11 64 9 649.0 0 9 65 11 649.0 0 11 66 10 649.0 0 10 67 14 649.0 0 14 68 11 649.0 0 11 69 20 649.0 0 20 70 39 649.0 0 39 71 102 649.0 0 102 72 48 649.0 0 48 73 92 649.0 0 92 74 11 649.0 0 11 75 16 649.0 0 16 76 13 649.0 0 13 77 27 649.0 0 27 78 37 649.0 0 37 79 75 649.0 0 75 80 42 649.0 0 42 81 33 649.0 0 33 82 29 649.0 0 29 83 56 649.0 0 56 84 70 649.0 0 70 85 55 649.0 0 55 86 71 649.0 0 71 87 36 649.0 0 36 88 55 649.0 0 55 89 61 649.0 0 61 90 81 649.0 0 81 91 71 649.0 0 71 92 92 649.0 0 92 93 79 649.0 0 79 94 1112 649.0 0 1112 95 690 649.0 0 690 96 1301 649.0 0 1301 97 327 649.0 0 327 98 49 649.0 0 49 99 49 649.0 0 49 100 31 649.0 0 31 101 54 649.0 0 54 102 28 649.0 0 28 103 28 649.0 0 28 104 25 649.0 0 25 105 29 649.0 0 29 106 62 649.0 0 62 107 35 649.0 0 35 108 32 649.0 0 32 109 22 649.0 0 22 110 24 649.0 0 24 111 36 649.0 0 36 112 50 649.0 0 50 113 222 649.0 0 222 114 417 649.0 0 417 115 280 649.0 0 280 116 240 649.0 0 240 117 59 649.0 0 59 118 32 649.0 0 32 119 48 649.0 0 48 120 53 649.0 0 53 121 26 649.0 0 26 122 51 649.0 0 51 123 46 649.0 0 46 124 38 649.0 0 38 125 37 649.0 0 37 126 41 649.0 0 41 127 39 649.0 0 39 128 82 649.0 0 82 129 63 649.0 0 63 130 51 649.0 0 51 131 61 649.0 0 61 132 61 649.0 0 61 133 109 649.0 0 109 134 107 649.0 0 107 135 417 649.0 0 417 136 464 649.0 0 464 137 350 649.0 0 350 138 301 649.0 0 301 139 172 649.0 0 172 140 139 649.0 0 139 141 65 649.0 0 65 142 76 649.0 0 76 143 73 649.0 0 73 144 65 649.0 0 65 145 46 649.0 0 46 146 37 649.0 0 37 147 28 649.0 0 28 148 37 649.0 0 37 149 36 649.0 0 36 150 38 649.0 0 38 151 46 649.0 0 46 152 33 649.0 0 33 153 35 649.0 0 35 154 33 649.0 0 33 155 57 649.0 0 57 156 53 649.0 0 53 157 48 649.0 0 48 158 40 649.0 0 40 159 23 649.0 0 23 160 27 649.0 0 27 161 22 649.0 0 22 162 66 649.0 0 66 163 69 649.0 0 69 164 81 649.0 0 81 165 50 649.0 0 50 166 39 649.0 0 39 167 99 649.0 0 99 168 135 649.0 0 135 169 87 649.0 0 87 170 75 649.0 0 75 171 33 649.0 0 33 172 40 649.0 0 40 173 30 649.0 0 30 174 41 649.0 0 41 175 41 649.0 0 41 176 24 649.0 0 24 177 25 649.0 0 25 178 53 649.0 0 53 179 83 649.0 0 83 180 54 649.0 0 54 181 83 649.0 0 83 182 27 649.0 0 27 183 42 649.0 0 42 184 94 649.0 0 94 185 117 649.0 0 117 186 84 649.0 0 84 187 74 649.0 0 74 188 52 649.0 0 52 189 56 649.0 0 56 190 50 649.0 0 50 191 49 649.0 0 49 192 50 649.0 0 50 193 33 649.0 0 33 194 55 649.0 0 55 195 39 649.0 0 39 196 37 649.0 0 37 197 48 649.0 0 48 198 42 649.0 0 42 199 69 649.0 0 69 200 46 649.0 0 46 201 71 649.0 0 71 202 92 649.0 0 92 203 60 649.0 0 60 204 150 649.0 0 150 205 151 649.0 0 151 206 145 649.0 0 145 207 119 649.0 0 119 208 56 649.0 0 56 209 50 649.0 0 50 210 75 649.0 0 75 211 57 649.0 0 57 212 48 649.0 0 48 213 32 649.0 0 32 214 42 649.0 0 42 215 69 649.0 0 69 216 66 649.0 0 66 217 40 649.0 0 40 218 53 649.0 0 53 219 39 649.0 0 39 220 54 649.0 0 54 221 58 649.0 0 58 222 101 649.0 0 101 223 97 649.0 0 97 224 1147 649.0 0 1147 225 1352 649.0 0 1352 226 1679 649.0 0 1679 227 1017 649.0 0 1017 228 371 649.0 0 371 229 45 649.0 0 45 230 39 649.0 0 39 231 50 649.0 0 50 232 50 649.0 0 50 233 44 649.0 0 44 234 49 649.0 0 49 235 54 649.0 0 54 236 83 649.0 0 83 237 68 649.0 0 68 238 29 649.0 0 29 239 101 649.0 0 101 240 42 649.0 0 42 241 66 649.0 0 66 242 27 649.0 0 27 243 24 649.0 0 24 244 40 649.0 0 40 245 36 649.0 0 36 246 46 649.0 0 46 247 27 649.0 0 27 248 42 649.0 0 42 249 24 649.0 0 24 250 33 649.0 0 33 251 28 649.0 0 28 252 27 649.0 0 27 253 64 649.0 0 64 254 53 649.0 0 53 255 60 649.0 0 60 256 24 649.0 0 24 257 52 649.0 0 52 258 42 649.0 0 42 259 84 649.0 0 84 260 61 649.0 0 61 261 47 649.0 0 47 262 32 649.0 0 32 263 36 649.0 0 36 264 68 649.0 0 68 265 37 649.0 0 37 266 95 649.0 0 95 267 34 649.0 0 34 268 49 649.0 0 49 269 53 649.0 0 53 270 26 649.0 0 26 271 15 649.0 0 15 272 38 649.0 0 38 273 32 649.0 0 32 274 31 649.0 0 31 275 39 649.0 0 39 276 30 649.0 0 30 277 25 649.0 0 25 278 30 649.0 0 30 279 33 649.0 0 33 280 74 649.0 0 74 281 40 649.0 0 40 282 58 649.0 0 58 283 31 649.0 0 31 284 22 649.0 0 22 285 28 649.0 0 28 286 30 649.0 0 30 287 31 649.0 0 31 288 28 649.0 0 28 289 34 649.0 0 34 290 37 649.0 0 37 291 23 649.0 0 23 292 32 649.0 0 32 293 45 649.0 0 45 294 56 649.0 0 56 295 68 649.0 0 68 296 42 649.0 0 42 297 45 649.0 0 45 298 46 649.0 0 46 299 60 649.0 0 60 300 38 649.0 0 38 301 125 649.0 0 125 Finished in 3.97 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 83,681 Reads with adapters: 50,785 (60.7%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 50,759 (60.7%) Total basepairs processed: 22,983,782 bp Total written (filtered): 13,994,939 bp (60.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50785 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 7 0.0 3 0 0 7 16 259 0.0 3 0 250 9 17 50200 0.0 3 49102 1060 36 2 18 128 0.0 3 22 102 4 19 28 0.0 3 3 1 22 2 20 36 0.0 3 0 0 0 36 21 1 0.0 3 0 0 0 1 24 4 0.0 3 0 0 0 4 27 2 0.0 3 0 0 0 2 30 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 1 35 12 0.0 3 0 0 1 11 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 52 4 0.0 3 0 0 0 4 53 1 0.0 3 0 0 0 1 54 4 0.0 3 0 0 0 4 56 4 0.0 3 0 0 0 4 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 65 2 0.0 3 0 0 0 2 68 8 0.0 3 0 0 0 8 76 1 0.0 3 0 0 0 1 78 9 0.0 3 0 0 0 9 79 5 0.0 3 0 0 0 5 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 1 98 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 11 0.0 3 0 0 2 9 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 1 149 2 0.0 3 0 1 0 1 150 1 0.0 3 0 0 1 151 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.80 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 50,759 Reads with adapters: 730 (1.4%) Reads written (passing filters): 50,759 (100.0%) Total basepairs processed: 13,994,939 bp Total written (filtered): 13,992,558 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 730 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.8% C: 23.7% G: 14.7% T: 9.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 599 793.1 0 599 4 89 198.3 0 89 5 29 49.6 0 29 6 10 12.4 0 10 7 2 3.1 0 2 9 1 0.2 0 1