This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.62 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 480,476 (18.1%) Reads that were too short: 25,106 (0.9%) Reads written (passing filters): 455,370 (17.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 85,853,896 bp (10.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 480476 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1463 649.0 0 1463 7 41749 649.0 0 41749 8 35280 649.0 0 35280 9 147 649.0 0 147 10 66 649.0 0 66 11 102 649.0 0 102 12 75 649.0 0 75 13 56 649.0 0 56 14 63 649.0 0 63 15 66 649.0 0 66 16 64 649.0 0 64 17 54 649.0 0 54 18 57 649.0 0 57 19 63 649.0 0 63 20 51 649.0 0 51 21 68 649.0 0 68 22 67 649.0 0 67 23 82 649.0 0 82 24 80 649.0 0 80 25 222 649.0 0 222 26 166 649.0 0 166 27 316 649.0 0 316 28 230 649.0 0 230 29 135 649.0 0 135 30 120 649.0 0 120 31 65 649.0 0 65 32 65 649.0 0 65 33 75 649.0 0 75 34 84 649.0 0 84 35 111 649.0 0 111 36 182 649.0 0 182 37 267 649.0 0 267 38 100 649.0 0 100 39 71 649.0 0 71 40 131 649.0 0 131 41 87 649.0 0 87 42 109 649.0 0 109 43 131 649.0 0 131 44 72 649.0 0 72 45 79 649.0 0 79 46 117 649.0 0 117 47 66 649.0 0 66 48 62 649.0 0 62 49 75 649.0 0 75 50 74 649.0 0 74 51 90 649.0 0 90 52 93 649.0 0 93 53 103 649.0 0 103 54 94 649.0 0 94 55 60 649.0 0 60 56 77 649.0 0 77 57 53 649.0 0 53 58 71 649.0 0 71 59 79 649.0 0 79 60 89 649.0 0 89 61 113 649.0 0 113 62 187 649.0 0 187 63 223 649.0 0 223 64 101 649.0 0 101 65 83 649.0 0 83 66 97 649.0 0 97 67 115 649.0 0 115 68 153 649.0 0 153 69 160 649.0 0 160 70 169 649.0 0 169 71 183 649.0 0 183 72 846 649.0 0 846 73 403 649.0 0 403 74 1260 649.0 0 1260 75 2224 649.0 0 2224 76 1768 649.0 0 1768 77 604 649.0 0 604 78 333 649.0 0 333 79 1093 649.0 0 1093 80 491 649.0 0 491 81 1676 649.0 0 1676 82 971 649.0 0 971 83 1848 649.0 0 1848 84 441 649.0 0 441 85 394 649.0 0 394 86 467 649.0 0 467 87 127 649.0 0 127 88 138 649.0 0 138 89 293 649.0 0 293 90 142 649.0 0 142 91 124 649.0 0 124 92 401 649.0 0 401 93 167 649.0 0 167 94 161 649.0 0 161 95 304 649.0 0 304 96 234 649.0 0 234 97 175 649.0 0 175 98 222 649.0 0 222 99 223 649.0 0 223 100 172 649.0 0 172 101 233 649.0 0 233 102 289 649.0 0 289 103 341 649.0 0 341 104 422 649.0 0 422 105 668 649.0 0 668 106 368 649.0 0 368 107 360 649.0 0 360 108 656 649.0 0 656 109 374 649.0 0 374 110 455 649.0 0 455 111 627 649.0 0 627 112 686 649.0 0 686 113 920 649.0 0 920 114 725 649.0 0 725 115 1001 649.0 0 1001 116 1259 649.0 0 1259 117 1861 649.0 0 1861 118 26085 649.0 0 26085 119 17435 649.0 0 17435 120 36365 649.0 0 36365 121 2486 649.0 0 2486 122 1842 649.0 0 1842 123 4655 649.0 0 4655 124 472 649.0 0 472 125 423 649.0 0 423 126 733 649.0 0 733 127 502 649.0 0 502 128 593 649.0 0 593 129 573 649.0 0 573 130 488 649.0 0 488 131 685 649.0 0 685 132 1097 649.0 0 1097 133 815 649.0 0 815 134 883 649.0 0 883 135 1077 649.0 0 1077 136 1182 649.0 0 1182 137 1343 649.0 0 1343 138 2279 649.0 0 2279 139 31824 649.0 0 31824 140 13877 649.0 0 13877 141 30670 649.0 0 30670 142 8109 649.0 0 8109 143 2854 649.0 0 2854 144 2974 649.0 0 2974 145 32902 649.0 0 32902 146 26150 649.0 0 26150 147 34368 649.0 0 34368 148 8388 649.0 0 8388 149 6155 649.0 0 6155 150 1082 649.0 0 1082 151 918 649.0 0 918 152 531 649.0 0 531 153 340 649.0 0 340 154 475 649.0 0 475 155 798 649.0 0 798 156 461 649.0 0 461 157 1770 649.0 0 1770 158 1563 649.0 0 1563 159 764 649.0 0 764 160 426 649.0 0 426 161 419 649.0 0 419 162 471 649.0 0 471 163 625 649.0 0 625 164 1245 649.0 0 1245 165 9519 649.0 0 9519 166 6720 649.0 0 6720 167 4305 649.0 0 4305 168 559 649.0 0 559 169 292 649.0 0 292 170 238 649.0 0 238 171 226 649.0 0 226 172 261 649.0 0 261 173 219 649.0 0 219 174 778 649.0 0 778 175 444 649.0 0 444 176 219 649.0 0 219 177 152 649.0 0 152 178 146 649.0 0 146 179 141 649.0 0 141 180 168 649.0 0 168 181 182 649.0 0 182 182 201 649.0 0 201 183 162 649.0 0 162 184 152 649.0 0 152 185 161 649.0 0 161 186 140 649.0 0 140 187 172 649.0 0 172 188 163 649.0 0 163 189 170 649.0 0 170 190 170 649.0 0 170 191 168 649.0 0 168 192 304 649.0 0 304 193 291 649.0 0 291 194 330 649.0 0 330 195 237 649.0 0 237 196 184 649.0 0 184 197 157 649.0 0 157 198 142 649.0 0 142 199 482 649.0 0 482 200 398 649.0 0 398 201 240 649.0 0 240 202 181 649.0 0 181 203 142 649.0 0 142 204 152 649.0 0 152 205 195 649.0 0 195 206 184 649.0 0 184 207 163 649.0 0 163 208 178 649.0 0 178 209 214 649.0 0 214 210 219 649.0 0 219 211 192 649.0 0 192 212 137 649.0 0 137 213 165 649.0 0 165 214 136 649.0 0 136 215 176 649.0 0 176 216 324 649.0 0 324 217 843 649.0 0 843 218 749 649.0 0 749 219 400 649.0 0 400 220 160 649.0 0 160 221 129 649.0 0 129 222 97 649.0 0 97 223 149 649.0 0 149 224 126 649.0 0 126 225 175 649.0 0 175 226 196 649.0 0 196 227 268 649.0 0 268 228 195 649.0 0 195 229 165 649.0 0 165 230 145 649.0 0 145 231 136 649.0 0 136 232 138 649.0 0 138 233 132 649.0 0 132 234 126 649.0 0 126 235 123 649.0 0 123 236 127 649.0 0 127 237 165 649.0 0 165 238 142 649.0 0 142 239 171 649.0 0 171 240 142 649.0 0 142 241 144 649.0 0 144 242 140 649.0 0 140 243 155 649.0 0 155 244 133 649.0 0 133 245 152 649.0 0 152 246 296 649.0 0 296 247 216 649.0 0 216 248 317 649.0 0 317 249 179 649.0 0 179 250 107 649.0 0 107 251 137 649.0 0 137 252 125 649.0 0 125 253 119 649.0 0 119 254 114 649.0 0 114 255 158 649.0 0 158 256 156 649.0 0 156 257 168 649.0 0 168 258 122 649.0 0 122 259 142 649.0 0 142 260 173 649.0 0 173 261 219 649.0 0 219 262 329 649.0 0 329 263 217 649.0 0 217 264 306 649.0 0 306 265 215 649.0 0 215 266 173 649.0 0 173 267 190 649.0 0 190 268 166 649.0 0 166 269 128 649.0 0 128 270 114 649.0 0 114 271 143 649.0 0 143 272 167 649.0 0 167 273 154 649.0 0 154 274 181 649.0 0 181 275 180 649.0 0 180 276 155 649.0 0 155 277 165 649.0 0 165 278 199 649.0 0 199 279 199 649.0 0 199 280 215 649.0 0 215 281 165 649.0 0 165 282 158 649.0 0 158 283 143 649.0 0 143 284 149 649.0 0 149 285 140 649.0 0 140 286 127 649.0 0 127 287 193 649.0 0 193 288 231 649.0 0 231 289 190 649.0 0 190 290 195 649.0 0 195 291 180 649.0 0 180 292 160 649.0 0 160 293 188 649.0 0 188 294 172 649.0 0 172 295 184 649.0 0 184 296 240 649.0 0 240 297 265 649.0 0 265 298 303 649.0 0 303 299 392 649.0 0 392 300 483 649.0 0 483 301 5823 649.0 0 5823 Finished in 18.02 s (40 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 455,370 Reads with adapters: 37,313 (8.2%) Reads that were too short: 232 (0.1%) Reads written (passing filters): 37,081 (8.1%) Total basepairs processed: 85,853,896 bp Total written (filtered): 10,076,372 bp (11.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37313 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 3 18 16 0.0 3 0 0 13 2 1 19 406 0.0 3 0 345 30 19 12 20 35465 0.0 4 33760 1420 223 45 17 21 122 0.0 4 25 81 8 4 4 22 56 0.0 4 20 14 14 5 3 23 578 0.0 4 448 23 4 74 29 24 7 0.0 4 1 3 0 1 2 25 2 0.0 4 0 0 0 1 1 26 5 0.0 4 0 0 0 3 2 28 2 0.0 4 0 0 0 0 2 32 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 42 6 0.0 4 0 0 0 0 6 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 1 49 4 0.0 4 0 0 0 1 3 50 15 0.0 4 0 0 0 0 15 53 21 0.0 4 0 0 0 0 21 54 2 0.0 4 0 0 0 0 2 55 2 0.0 4 0 0 0 0 2 56 2 0.0 4 0 0 0 1 1 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 6 0.0 4 0 0 0 0 6 76 6 0.0 4 0 0 0 0 6 77 8 0.0 4 0 0 1 0 7 78 9 0.0 4 0 0 0 0 9 79 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 1 84 1 0.0 4 0 0 1 85 6 0.0 4 0 0 0 1 5 86 5 0.0 4 0 0 0 0 5 87 3 0.0 4 0 0 0 0 3 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 7 0.0 4 0 0 0 1 6 97 18 0.0 4 0 0 0 0 18 98 7 0.0 4 0 0 0 0 7 99 2 0.0 4 0 0 0 0 2 100 10 0.0 4 0 0 0 0 10 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 3 0.0 4 0 0 0 0 3 108 5 0.0 4 0 0 0 1 4 109 4 0.0 4 0 0 0 0 4 110 1 0.0 4 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 3 0.0 4 0 0 0 1 2 118 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 9 0.0 4 0 0 0 0 9 125 5 0.0 4 0 0 0 1 4 126 13 0.0 4 0 0 0 0 13 127 312 0.0 4 0 0 0 0 312 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 1 137 1 0.0 4 0 1 138 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 143 29 0.0 4 0 0 0 0 29 145 3 0.0 4 0 0 0 0 3 146 1 0.0 4 0 0 0 0 1 147 4 0.0 4 0 0 0 0 4 148 3 0.0 4 0 0 0 0 3 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 5 0.0 4 3 1 0 0 1 153 7 0.0 4 1 0 0 0 6 154 5 0.0 4 0 1 0 1 3 155 8 0.0 4 0 1 0 0 7 156 1 0.0 4 0 0 0 0 1 157 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 169 2 0.0 4 0 0 0 0 2 176 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 0 0 0 2 180 1 0.0 4 1 186 2 0.0 4 0 0 0 0 2 187 2 0.0 4 0 0 0 0 2 194 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 288 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.32 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 37,081 Reads with adapters: 6,186 (16.7%) Reads written (passing filters): 37,081 (100.0%) Total basepairs processed: 10,076,372 bp Total written (filtered): 10,002,986 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6186 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 8.3% G: 86.3% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 54 579.4 0 54 4 12 144.8 0 12 5 2 36.2 0 2 6 1 9.1 0 1 9 138 0.1 0 9 129 10 783 0.0 1 63 720 11 5090 0.0 1 191 4899 12 43 0.0 1 1 42 13 9 0.0 1 0 9 14 10 0.0 1 0 10 15 1 0.0 1 0 1 18 2 0.0 1 2 22 2 0.0 1 1 1 35 1 0.0 1 1 146 1 0.0 1 1 151 12 0.0 1 11 1 170 1 0.0 1 1 190 1 0.0 1 0 1 209 20 0.0 1 11 9 210 1 0.0 1 1 229 1 0.0 1 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.