This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.14 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 478,339 (18.0%) Reads that were too short: 24,901 (0.9%) Reads written (passing filters): 453,438 (17.1%) Total basepairs processed: 800,111,879 bp Total written (filtered): 85,246,367 bp (10.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 478339 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1155 649.0 0 1155 7 37604 649.0 0 37604 8 42056 649.0 0 42056 9 111 649.0 0 111 10 66 649.0 0 66 11 74 649.0 0 74 12 87 649.0 0 87 13 70 649.0 0 70 14 68 649.0 0 68 15 79 649.0 0 79 16 67 649.0 0 67 17 54 649.0 0 54 18 76 649.0 0 76 19 42 649.0 0 42 20 46 649.0 0 46 21 50 649.0 0 50 22 68 649.0 0 68 23 60 649.0 0 60 24 60 649.0 0 60 25 102 649.0 0 102 26 135 649.0 0 135 27 98 649.0 0 98 28 270 649.0 0 270 29 209 649.0 0 209 30 168 649.0 0 168 31 73 649.0 0 73 32 67 649.0 0 67 33 64 649.0 0 64 34 83 649.0 0 83 35 120 649.0 0 120 36 192 649.0 0 192 37 285 649.0 0 285 38 94 649.0 0 94 39 96 649.0 0 96 40 149 649.0 0 149 41 110 649.0 0 110 42 143 649.0 0 143 43 154 649.0 0 154 44 84 649.0 0 84 45 90 649.0 0 90 46 102 649.0 0 102 47 56 649.0 0 56 48 93 649.0 0 93 49 82 649.0 0 82 50 89 649.0 0 89 51 136 649.0 0 136 52 80 649.0 0 80 53 110 649.0 0 110 54 118 649.0 0 118 55 87 649.0 0 87 56 76 649.0 0 76 57 66 649.0 0 66 58 93 649.0 0 93 59 82 649.0 0 82 60 102 649.0 0 102 61 144 649.0 0 144 62 137 649.0 0 137 63 212 649.0 0 212 64 103 649.0 0 103 65 73 649.0 0 73 66 93 649.0 0 93 67 93 649.0 0 93 68 124 649.0 0 124 69 112 649.0 0 112 70 171 649.0 0 171 71 104 649.0 0 104 72 118 649.0 0 118 73 156 649.0 0 156 74 191 649.0 0 191 75 2211 649.0 0 2211 76 220 649.0 0 220 77 263 649.0 0 263 78 150 649.0 0 150 79 343 649.0 0 343 80 414 649.0 0 414 81 1394 649.0 0 1394 82 659 649.0 0 659 83 1437 649.0 0 1437 84 478 649.0 0 478 85 365 649.0 0 365 86 472 649.0 0 472 87 292 649.0 0 292 88 222 649.0 0 222 89 261 649.0 0 261 90 285 649.0 0 285 91 199 649.0 0 199 92 270 649.0 0 270 93 260 649.0 0 260 94 274 649.0 0 274 95 256 649.0 0 256 96 240 649.0 0 240 97 255 649.0 0 255 98 217 649.0 0 217 99 239 649.0 0 239 100 245 649.0 0 245 101 352 649.0 0 352 102 290 649.0 0 290 103 392 649.0 0 392 104 471 649.0 0 471 105 745 649.0 0 745 106 338 649.0 0 338 107 361 649.0 0 361 108 653 649.0 0 653 109 382 649.0 0 382 110 391 649.0 0 391 111 622 649.0 0 622 112 680 649.0 0 680 113 767 649.0 0 767 114 591 649.0 0 591 115 932 649.0 0 932 116 977 649.0 0 977 117 1529 649.0 0 1529 118 22606 649.0 0 22606 119 16065 649.0 0 16065 120 36397 649.0 0 36397 121 2250 649.0 0 2250 122 1780 649.0 0 1780 123 4617 649.0 0 4617 124 471 649.0 0 471 125 425 649.0 0 425 126 763 649.0 0 763 127 568 649.0 0 568 128 563 649.0 0 563 129 680 649.0 0 680 130 588 649.0 0 588 131 666 649.0 0 666 132 1114 649.0 0 1114 133 771 649.0 0 771 134 818 649.0 0 818 135 1049 649.0 0 1049 136 1218 649.0 0 1218 137 1218 649.0 0 1218 138 1935 649.0 0 1935 139 32955 649.0 0 32955 140 14446 649.0 0 14446 141 33636 649.0 0 33636 142 8185 649.0 0 8185 143 2932 649.0 0 2932 144 2681 649.0 0 2681 145 32642 649.0 0 32642 146 26754 649.0 0 26754 147 33554 649.0 0 33554 148 7860 649.0 0 7860 149 5840 649.0 0 5840 150 1022 649.0 0 1022 151 979 649.0 0 979 152 527 649.0 0 527 153 398 649.0 0 398 154 569 649.0 0 569 155 1085 649.0 0 1085 156 604 649.0 0 604 157 1976 649.0 0 1976 158 1824 649.0 0 1824 159 895 649.0 0 895 160 472 649.0 0 472 161 420 649.0 0 420 162 470 649.0 0 470 163 541 649.0 0 541 164 1177 649.0 0 1177 165 10377 649.0 0 10377 166 7921 649.0 0 7921 167 4739 649.0 0 4739 168 419 649.0 0 419 169 251 649.0 0 251 170 185 649.0 0 185 171 239 649.0 0 239 172 249 649.0 0 249 173 201 649.0 0 201 174 914 649.0 0 914 175 507 649.0 0 507 176 263 649.0 0 263 177 161 649.0 0 161 178 171 649.0 0 171 179 179 649.0 0 179 180 149 649.0 0 149 181 193 649.0 0 193 182 202 649.0 0 202 183 190 649.0 0 190 184 197 649.0 0 197 185 187 649.0 0 187 186 187 649.0 0 187 187 185 649.0 0 185 188 190 649.0 0 190 189 213 649.0 0 213 190 189 649.0 0 189 191 195 649.0 0 195 192 263 649.0 0 263 193 257 649.0 0 257 194 352 649.0 0 352 195 248 649.0 0 248 196 247 649.0 0 247 197 202 649.0 0 202 198 189 649.0 0 189 199 492 649.0 0 492 200 521 649.0 0 521 201 332 649.0 0 332 202 182 649.0 0 182 203 177 649.0 0 177 204 184 649.0 0 184 205 160 649.0 0 160 206 194 649.0 0 194 207 191 649.0 0 191 208 190 649.0 0 190 209 208 649.0 0 208 210 222 649.0 0 222 211 241 649.0 0 241 212 151 649.0 0 151 213 177 649.0 0 177 214 190 649.0 0 190 215 166 649.0 0 166 216 365 649.0 0 365 217 1096 649.0 0 1096 218 903 649.0 0 903 219 551 649.0 0 551 220 177 649.0 0 177 221 159 649.0 0 159 222 146 649.0 0 146 223 157 649.0 0 157 224 132 649.0 0 132 225 140 649.0 0 140 226 187 649.0 0 187 227 216 649.0 0 216 228 218 649.0 0 218 229 128 649.0 0 128 230 139 649.0 0 139 231 142 649.0 0 142 232 129 649.0 0 129 233 132 649.0 0 132 234 135 649.0 0 135 235 130 649.0 0 130 236 132 649.0 0 132 237 164 649.0 0 164 238 165 649.0 0 165 239 168 649.0 0 168 240 122 649.0 0 122 241 170 649.0 0 170 242 143 649.0 0 143 243 139 649.0 0 139 244 145 649.0 0 145 245 123 649.0 0 123 246 198 649.0 0 198 247 197 649.0 0 197 248 242 649.0 0 242 249 163 649.0 0 163 250 135 649.0 0 135 251 119 649.0 0 119 252 143 649.0 0 143 253 125 649.0 0 125 254 132 649.0 0 132 255 172 649.0 0 172 256 158 649.0 0 158 257 179 649.0 0 179 258 165 649.0 0 165 259 150 649.0 0 150 260 168 649.0 0 168 261 176 649.0 0 176 262 192 649.0 0 192 263 148 649.0 0 148 264 212 649.0 0 212 265 156 649.0 0 156 266 143 649.0 0 143 267 187 649.0 0 187 268 145 649.0 0 145 269 124 649.0 0 124 270 169 649.0 0 169 271 157 649.0 0 157 272 137 649.0 0 137 273 153 649.0 0 153 274 136 649.0 0 136 275 144 649.0 0 144 276 121 649.0 0 121 277 143 649.0 0 143 278 185 649.0 0 185 279 155 649.0 0 155 280 145 649.0 0 145 281 150 649.0 0 150 282 147 649.0 0 147 283 146 649.0 0 146 284 147 649.0 0 147 285 143 649.0 0 143 286 135 649.0 0 135 287 203 649.0 0 203 288 241 649.0 0 241 289 175 649.0 0 175 290 195 649.0 0 195 291 212 649.0 0 212 292 143 649.0 0 143 293 174 649.0 0 174 294 213 649.0 0 213 295 192 649.0 0 192 296 238 649.0 0 238 297 237 649.0 0 237 298 305 649.0 0 305 299 359 649.0 0 359 300 422 649.0 0 422 301 5664 649.0 0 5664 Finished in 15.87 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 453,438 Reads with adapters: 50,182 (11.1%) Reads that were too short: 371 (0.1%) Reads written (passing filters): 49,811 (11.0%) Total basepairs processed: 85,246,367 bp Total written (filtered): 11,712,322 bp (13.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50182 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 8 16 193 0.0 3 0 188 2 3 17 36918 0.0 3 36171 717 24 6 18 84 0.0 3 17 63 2 2 19 21 0.0 3 2 0 15 4 20 19 0.0 3 1 1 0 17 21 2 0.0 3 0 0 0 2 23 11 0.0 3 0 0 0 11 24 3 0.0 3 0 0 0 3 25 1 0.0 3 0 0 0 1 26 1853 0.0 3 0 0 4 1849 27 6 0.0 3 0 0 0 6 28 2 0.0 3 0 0 0 2 29 100 0.0 3 0 0 2 98 30 4 0.0 3 0 0 0 4 31 1 0.0 3 1 32 2 0.0 3 0 0 0 2 33 3 0.0 3 0 0 0 3 39 1 0.0 3 0 0 0 1 42 5 0.0 3 0 0 0 5 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 0 3 48 2 0.0 3 0 0 0 2 49 3 0.0 3 0 0 0 3 50 30 0.0 3 0 0 0 30 51 3 0.0 3 0 0 0 3 52 155 0.0 3 0 0 0 155 53 454 0.0 3 0 0 1 453 54 6 0.0 3 0 0 0 6 55 5 0.0 3 0 0 0 5 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 65 4 0.0 3 0 0 0 4 67 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 70 4 0.0 3 0 0 0 4 71 16 0.0 3 0 0 0 16 72 9326 0.0 3 0 0 68 9258 73 109 0.0 3 0 0 2 107 74 387 0.0 3 0 0 2 385 76 11 0.0 3 0 0 1 10 77 44 0.0 3 0 0 0 44 78 4 0.0 3 1 0 0 3 79 3 0.0 3 0 1 0 2 80 2 0.0 3 0 1 0 1 81 2 0.0 3 0 0 1 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 5 0.0 3 0 0 0 5 86 6 0.0 3 0 0 0 6 87 3 0.0 3 0 0 0 3 88 3 0.0 3 0 0 0 3 89 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 106 6 0.0 3 0 0 0 6 107 2 0.0 3 0 0 0 2 109 8 0.0 3 0 0 0 8 110 9 0.0 3 0 0 0 9 111 10 0.0 3 0 0 0 10 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 3 0.0 3 1 0 0 2 127 3 0.0 3 1 0 0 2 128 21 0.0 3 0 0 1 20 129 8 0.0 3 0 0 1 7 131 1 0.0 3 0 0 0 1 132 4 0.0 3 0 0 0 4 133 23 0.0 3 0 0 0 23 134 4 0.0 3 0 0 0 4 135 3 0.0 3 0 0 0 3 137 2 0.0 3 0 0 0 2 138 50 0.0 3 0 0 1 49 139 14 0.0 3 0 0 0 14 140 5 0.0 3 0 0 0 5 141 53 0.0 3 0 0 0 53 142 5 0.0 3 0 0 1 4 143 1 0.0 3 0 0 0 1 145 5 0.0 3 0 0 0 5 146 2 0.0 3 0 0 0 2 148 2 0.0 3 1 0 0 1 150 1 0.0 3 0 0 0 1 151 4 0.0 3 2 0 0 2 152 1 0.0 3 0 0 0 1 156 14 0.0 3 0 1 0 13 162 1 0.0 3 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 3 0.0 3 0 0 0 3 166 4 0.0 3 0 0 0 4 168 2 0.0 3 0 0 0 2 169 1 0.0 3 1 171 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 177 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 1 1 188 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 1 1 192 4 0.0 3 0 0 0 4 194 1 0.0 3 0 0 0 1 206 2 0.0 3 0 1 0 1 208 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 220 1 0.0 3 0 0 0 1 230 6 0.0 3 0 0 0 6 231 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 235 1 0.0 3 0 1 270 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 3 0.0 3 0 0 0 3 282 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.61 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 49,811 Reads with adapters: 362 (0.7%) Reads written (passing filters): 49,811 (100.0%) Total basepairs processed: 11,712,322 bp Total written (filtered): 11,711,102 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 362 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.5% C: 14.1% G: 61.9% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 233 778.3 0 233 4 127 194.6 0 127 5 1 48.6 0 1 8 1 0.8 0 1