This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.12 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 70,103 (2.6%) Reads that were too short: 18,845 (0.7%) Reads written (passing filters): 51,258 (1.9%) Total basepairs processed: 800,111,879 bp Total written (filtered): 9,852,673 bp (1.2%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 70103 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 309 649.0 0 309 7 5319 649.0 0 5319 8 510 649.0 0 510 9 5978 649.0 0 5978 10 31 649.0 0 31 11 32 649.0 0 32 12 30 649.0 0 30 13 24 649.0 0 24 14 34 649.0 0 34 15 28 649.0 0 28 16 24 649.0 0 24 17 19 649.0 0 19 18 25 649.0 0 25 19 25 649.0 0 25 20 32 649.0 0 32 21 28 649.0 0 28 22 27 649.0 0 27 23 34 649.0 0 34 24 29 649.0 0 29 25 29 649.0 0 29 26 31 649.0 0 31 27 24 649.0 0 24 28 39 649.0 0 39 29 32 649.0 0 32 30 49 649.0 0 49 31 39 649.0 0 39 32 61 649.0 0 61 33 631 649.0 0 631 34 376 649.0 0 376 35 310 649.0 0 310 36 33 649.0 0 33 37 26 649.0 0 26 38 38 649.0 0 38 39 32 649.0 0 32 40 29 649.0 0 29 41 32 649.0 0 32 42 29 649.0 0 29 43 34 649.0 0 34 44 40 649.0 0 40 45 88 649.0 0 88 46 87 649.0 0 87 47 70 649.0 0 70 48 52 649.0 0 52 49 67 649.0 0 67 50 52 649.0 0 52 51 47 649.0 0 47 52 43 649.0 0 43 53 37 649.0 0 37 54 41 649.0 0 41 55 48 649.0 0 48 56 82 649.0 0 82 57 367 649.0 0 367 58 149 649.0 0 149 59 114 649.0 0 114 60 46 649.0 0 46 61 41 649.0 0 41 62 43 649.0 0 43 63 42 649.0 0 42 64 73 649.0 0 73 65 150 649.0 0 150 66 37 649.0 0 37 67 46 649.0 0 46 68 57 649.0 0 57 69 45 649.0 0 45 70 45 649.0 0 45 71 44 649.0 0 44 72 241 649.0 0 241 73 106 649.0 0 106 74 114 649.0 0 114 75 38 649.0 0 38 76 41 649.0 0 41 77 54 649.0 0 54 78 65 649.0 0 65 79 52 649.0 0 52 80 44 649.0 0 44 81 39 649.0 0 39 82 57 649.0 0 57 83 211 649.0 0 211 84 122 649.0 0 122 85 207 649.0 0 207 86 751 649.0 0 751 87 374 649.0 0 374 88 81 649.0 0 81 89 63 649.0 0 63 90 96 649.0 0 96 91 68 649.0 0 68 92 51 649.0 0 51 93 55 649.0 0 55 94 84 649.0 0 84 95 67 649.0 0 67 96 100 649.0 0 100 97 64 649.0 0 64 98 49 649.0 0 49 99 64 649.0 0 64 100 48 649.0 0 48 101 48 649.0 0 48 102 45 649.0 0 45 103 56 649.0 0 56 104 104 649.0 0 104 105 140 649.0 0 140 106 156 649.0 0 156 107 1242 649.0 0 1242 108 975 649.0 0 975 109 1810 649.0 0 1810 110 60 649.0 0 60 111 38 649.0 0 38 112 39 649.0 0 39 113 154 649.0 0 154 114 38 649.0 0 38 115 34 649.0 0 34 116 44 649.0 0 44 117 28 649.0 0 28 118 30 649.0 0 30 119 72 649.0 0 72 120 46 649.0 0 46 121 68 649.0 0 68 122 85 649.0 0 85 123 171 649.0 0 171 124 64 649.0 0 64 125 42 649.0 0 42 126 70 649.0 0 70 127 510 649.0 0 510 128 433 649.0 0 433 129 707 649.0 0 707 130 349 649.0 0 349 131 60 649.0 0 60 132 112 649.0 0 112 133 50 649.0 0 50 134 67 649.0 0 67 135 103 649.0 0 103 136 138 649.0 0 138 137 124 649.0 0 124 138 94 649.0 0 94 139 46 649.0 0 46 140 135 649.0 0 135 141 186 649.0 0 186 142 186 649.0 0 186 143 667 649.0 0 667 144 334 649.0 0 334 145 696 649.0 0 696 146 110 649.0 0 110 147 284 649.0 0 284 148 150 649.0 0 150 149 157 649.0 0 157 150 86 649.0 0 86 151 98 649.0 0 98 152 347 649.0 0 347 153 810 649.0 0 810 154 498 649.0 0 498 155 512 649.0 0 512 156 461 649.0 0 461 157 115 649.0 0 115 158 97 649.0 0 97 159 159 649.0 0 159 160 139 649.0 0 139 161 121 649.0 0 121 162 106 649.0 0 106 163 110 649.0 0 110 164 60 649.0 0 60 165 88 649.0 0 88 166 88 649.0 0 88 167 87 649.0 0 87 168 86 649.0 0 86 169 222 649.0 0 222 170 108 649.0 0 108 171 110 649.0 0 110 172 86 649.0 0 86 173 108 649.0 0 108 174 184 649.0 0 184 175 177 649.0 0 177 176 120 649.0 0 120 177 94 649.0 0 94 178 61 649.0 0 61 179 92 649.0 0 92 180 120 649.0 0 120 181 153 649.0 0 153 182 1134 649.0 0 1134 183 321 649.0 0 321 184 323 649.0 0 323 185 377 649.0 0 377 186 3683 649.0 0 3683 187 5305 649.0 0 5305 188 2066 649.0 0 2066 189 509 649.0 0 509 190 47 649.0 0 47 191 42 649.0 0 42 192 51 649.0 0 51 193 45 649.0 0 45 194 66 649.0 0 66 195 52 649.0 0 52 196 51 649.0 0 51 197 57 649.0 0 57 198 37 649.0 0 37 199 49 649.0 0 49 200 55 649.0 0 55 201 57 649.0 0 57 202 83 649.0 0 83 203 89 649.0 0 89 204 127 649.0 0 127 205 80 649.0 0 80 206 80 649.0 0 80 207 73 649.0 0 73 208 135 649.0 0 135 209 112 649.0 0 112 210 83 649.0 0 83 211 54 649.0 0 54 212 59 649.0 0 59 213 113 649.0 0 113 214 49 649.0 0 49 215 77 649.0 0 77 216 104 649.0 0 104 217 347 649.0 0 347 218 618 649.0 0 618 219 393 649.0 0 393 220 70 649.0 0 70 221 90 649.0 0 90 222 205 649.0 0 205 223 151 649.0 0 151 224 82 649.0 0 82 225 73 649.0 0 73 226 94 649.0 0 94 227 140 649.0 0 140 228 63 649.0 0 63 229 77 649.0 0 77 230 59 649.0 0 59 231 68 649.0 0 68 232 91 649.0 0 91 233 102 649.0 0 102 234 94 649.0 0 94 235 100 649.0 0 100 236 97 649.0 0 97 237 57 649.0 0 57 238 63 649.0 0 63 239 81 649.0 0 81 240 69 649.0 0 69 241 132 649.0 0 132 242 164 649.0 0 164 243 253 649.0 0 253 244 131 649.0 0 131 245 182 649.0 0 182 246 99 649.0 0 99 247 81 649.0 0 81 248 94 649.0 0 94 249 77 649.0 0 77 250 464 649.0 0 464 251 225 649.0 0 225 252 541 649.0 0 541 253 171 649.0 0 171 254 252 649.0 0 252 255 108 649.0 0 108 256 103 649.0 0 103 257 84 649.0 0 84 258 79 649.0 0 79 259 102 649.0 0 102 260 150 649.0 0 150 261 459 649.0 0 459 262 350 649.0 0 350 263 155 649.0 0 155 264 203 649.0 0 203 265 73 649.0 0 73 266 86 649.0 0 86 267 106 649.0 0 106 268 93 649.0 0 93 269 133 649.0 0 133 270 181 649.0 0 181 271 228 649.0 0 228 272 316 649.0 0 316 273 207 649.0 0 207 274 96 649.0 0 96 275 82 649.0 0 82 276 95 649.0 0 95 277 106 649.0 0 106 278 208 649.0 0 208 279 215 649.0 0 215 280 594 649.0 0 594 281 554 649.0 0 554 282 584 649.0 0 584 283 142 649.0 0 142 284 86 649.0 0 86 285 128 649.0 0 128 286 132 649.0 0 132 287 97 649.0 0 97 288 179 649.0 0 179 289 215 649.0 0 215 290 225 649.0 0 225 291 334 649.0 0 334 292 116 649.0 0 116 293 132 649.0 0 132 294 220 649.0 0 220 295 258 649.0 0 258 296 589 649.0 0 589 297 1159 649.0 0 1159 298 682 649.0 0 682 299 610 649.0 0 610 300 193 649.0 0 193 301 130 649.0 0 130 Finished in 2.21 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 51,258 Reads with adapters: 5,677 (11.1%) Reads that were too short: 36 (0.1%) Reads written (passing filters): 5,641 (11.0%) Total basepairs processed: 9,852,673 bp Total written (filtered): 1,522,058 bp (15.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5677 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 1 0 1 19 77 0.0 3 0 72 4 0 1 20 5326 0.0 4 5090 202 28 5 1 21 12 0.0 4 2 9 1 22 4 0.0 4 3 0 1 23 3 0.0 4 0 0 0 2 1 25 4 0.0 4 0 0 0 0 4 31 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 1 60 1 0.0 4 0 0 0 0 1 62 4 0.0 4 0 0 0 0 4 63 1 0.0 4 0 0 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 1 71 1 0.0 4 0 0 0 0 1 81 1 0.0 4 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 195 0.0 4 0 0 0 0 195 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 147 1 0.0 4 1 149 1 0.0 4 1 157 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 238 13 0.0 4 0 0 0 1 12 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 5,641 Reads with adapters: 339 (6.0%) Reads written (passing filters): 5,641 (100.0%) Total basepairs processed: 1,522,058 bp Total written (filtered): 1,518,060 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 339 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.4% C: 10.9% G: 81.7% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 24 88.1 0 24 4 2 22.0 0 2 9 2 0.0 0 0 2 10 3 0.0 1 0 3 11 240 0.0 1 13 227 12 56 0.0 1 1 55 13 6 0.0 1 0 6 14 2 0.0 1 0 2 15 1 0.0 1 0 1 74 1 0.0 1 0 1 153 1 0.0 1 1 210 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.