This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.91 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 76,865 (2.9%) Reads that were too short: 10,695 (0.4%) Reads written (passing filters): 66,170 (2.5%) Total basepairs processed: 800,111,879 bp Total written (filtered): 16,588,106 bp (2.1%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 76865 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 324 649.0 0 324 7 1140 649.0 0 1140 8 21981 649.0 0 21981 9 18248 649.0 0 18248 10 53 649.0 0 53 11 13 649.0 0 13 12 22 649.0 0 22 13 17 649.0 0 17 14 13 649.0 0 13 15 18 649.0 0 18 16 12 649.0 0 12 17 11 649.0 0 11 18 12 649.0 0 12 19 24 649.0 0 24 20 9 649.0 0 9 21 7 649.0 0 7 22 16 649.0 0 16 23 5 649.0 0 5 24 6 649.0 0 6 25 16 649.0 0 16 26 13 649.0 0 13 27 10 649.0 0 10 28 11 649.0 0 11 29 13 649.0 0 13 30 13 649.0 0 13 31 10 649.0 0 10 32 14 649.0 0 14 33 8 649.0 0 8 34 10 649.0 0 10 35 8 649.0 0 8 36 12 649.0 0 12 37 27 649.0 0 27 38 14 649.0 0 14 39 8 649.0 0 8 40 25 649.0 0 25 41 15 649.0 0 15 42 34 649.0 0 34 43 6 649.0 0 6 44 23 649.0 0 23 45 32 649.0 0 32 46 31 649.0 0 31 47 86 649.0 0 86 48 18 649.0 0 18 49 227 649.0 0 227 50 42 649.0 0 42 51 12 649.0 0 12 52 12 649.0 0 12 53 18 649.0 0 18 54 18 649.0 0 18 55 19 649.0 0 19 56 17 649.0 0 17 57 10 649.0 0 10 58 38 649.0 0 38 59 32 649.0 0 32 60 46 649.0 0 46 61 40 649.0 0 40 62 27 649.0 0 27 63 14 649.0 0 14 64 13 649.0 0 13 65 10 649.0 0 10 66 268 649.0 0 268 67 83 649.0 0 83 68 158 649.0 0 158 69 11 649.0 0 11 70 17 649.0 0 17 71 29 649.0 0 29 72 12 649.0 0 12 73 25 649.0 0 25 74 12 649.0 0 12 75 8 649.0 0 8 76 12 649.0 0 12 77 14 649.0 0 14 78 11 649.0 0 11 79 15 649.0 0 15 80 16 649.0 0 16 81 16 649.0 0 16 82 35 649.0 0 35 83 46 649.0 0 46 84 91 649.0 0 91 85 75 649.0 0 75 86 33 649.0 0 33 87 21 649.0 0 21 88 47 649.0 0 47 89 24 649.0 0 24 90 21 649.0 0 21 91 373 649.0 0 373 92 71 649.0 0 71 93 60 649.0 0 60 94 59 649.0 0 59 95 27 649.0 0 27 96 12 649.0 0 12 97 29 649.0 0 29 98 20 649.0 0 20 99 18 649.0 0 18 100 31 649.0 0 31 101 20 649.0 0 20 102 36 649.0 0 36 103 73 649.0 0 73 104 88 649.0 0 88 105 44 649.0 0 44 106 37 649.0 0 37 107 55 649.0 0 55 108 36 649.0 0 36 109 49 649.0 0 49 110 65 649.0 0 65 111 150 649.0 0 150 112 122 649.0 0 122 113 287 649.0 0 287 114 118 649.0 0 118 115 407 649.0 0 407 116 694 649.0 0 694 117 5243 649.0 0 5243 118 3682 649.0 0 3682 119 3743 649.0 0 3743 120 40 649.0 0 40 121 25 649.0 0 25 122 28 649.0 0 28 123 15 649.0 0 15 124 31 649.0 0 31 125 45 649.0 0 45 126 46 649.0 0 46 127 47 649.0 0 47 128 33 649.0 0 33 129 37 649.0 0 37 130 47 649.0 0 47 131 68 649.0 0 68 132 65 649.0 0 65 133 93 649.0 0 93 134 37 649.0 0 37 135 33 649.0 0 33 136 28 649.0 0 28 137 40 649.0 0 40 138 72 649.0 0 72 139 46 649.0 0 46 140 66 649.0 0 66 141 33 649.0 0 33 142 33 649.0 0 33 143 22 649.0 0 22 144 43 649.0 0 43 145 67 649.0 0 67 146 99 649.0 0 99 147 58 649.0 0 58 148 64 649.0 0 64 149 64 649.0 0 64 150 62 649.0 0 62 151 643 649.0 0 643 152 692 649.0 0 692 153 586 649.0 0 586 154 234 649.0 0 234 155 64 649.0 0 64 156 47 649.0 0 47 157 29 649.0 0 29 158 28 649.0 0 28 159 39 649.0 0 39 160 45 649.0 0 45 161 53 649.0 0 53 162 160 649.0 0 160 163 106 649.0 0 106 164 99 649.0 0 99 165 37 649.0 0 37 166 51 649.0 0 51 167 35 649.0 0 35 168 24 649.0 0 24 169 43 649.0 0 43 170 61 649.0 0 61 171 56 649.0 0 56 172 39 649.0 0 39 173 44 649.0 0 44 174 29 649.0 0 29 175 29 649.0 0 29 176 26 649.0 0 26 177 39 649.0 0 39 178 42 649.0 0 42 179 104 649.0 0 104 180 132 649.0 0 132 181 107 649.0 0 107 182 126 649.0 0 126 183 135 649.0 0 135 184 93 649.0 0 93 185 214 649.0 0 214 186 25 649.0 0 25 187 24 649.0 0 24 188 24 649.0 0 24 189 45 649.0 0 45 190 61 649.0 0 61 191 79 649.0 0 79 192 58 649.0 0 58 193 31 649.0 0 31 194 24 649.0 0 24 195 35 649.0 0 35 196 31 649.0 0 31 197 49 649.0 0 49 198 48 649.0 0 48 199 50 649.0 0 50 200 34 649.0 0 34 201 42 649.0 0 42 202 37 649.0 0 37 203 48 649.0 0 48 204 48 649.0 0 48 205 41 649.0 0 41 206 35 649.0 0 35 207 35 649.0 0 35 208 43 649.0 0 43 209 31 649.0 0 31 210 62 649.0 0 62 211 30 649.0 0 30 212 84 649.0 0 84 213 55 649.0 0 55 214 71 649.0 0 71 215 56 649.0 0 56 216 76 649.0 0 76 217 247 649.0 0 247 218 266 649.0 0 266 219 361 649.0 0 361 220 256 649.0 0 256 221 90 649.0 0 90 222 40 649.0 0 40 223 101 649.0 0 101 224 76 649.0 0 76 225 99 649.0 0 99 226 232 649.0 0 232 227 86 649.0 0 86 228 209 649.0 0 209 229 131 649.0 0 131 230 53 649.0 0 53 231 280 649.0 0 280 232 157 649.0 0 157 233 252 649.0 0 252 234 229 649.0 0 229 235 130 649.0 0 130 236 109 649.0 0 109 237 91 649.0 0 91 238 102 649.0 0 102 239 100 649.0 0 100 240 69 649.0 0 69 241 27 649.0 0 27 242 64 649.0 0 64 243 58 649.0 0 58 244 79 649.0 0 79 245 208 649.0 0 208 246 197 649.0 0 197 247 286 649.0 0 286 248 145 649.0 0 145 249 75 649.0 0 75 250 136 649.0 0 136 251 114 649.0 0 114 252 124 649.0 0 124 253 170 649.0 0 170 254 148 649.0 0 148 255 177 649.0 0 177 256 82 649.0 0 82 257 96 649.0 0 96 258 50 649.0 0 50 259 67 649.0 0 67 260 42 649.0 0 42 261 56 649.0 0 56 262 27 649.0 0 27 263 57 649.0 0 57 264 64 649.0 0 64 265 78 649.0 0 78 266 101 649.0 0 101 267 77 649.0 0 77 268 102 649.0 0 102 269 38 649.0 0 38 270 76 649.0 0 76 271 84 649.0 0 84 272 93 649.0 0 93 273 109 649.0 0 109 274 68 649.0 0 68 275 57 649.0 0 57 276 47 649.0 0 47 277 77 649.0 0 77 278 136 649.0 0 136 279 157 649.0 0 157 280 151 649.0 0 151 281 95 649.0 0 95 282 103 649.0 0 103 283 96 649.0 0 96 284 108 649.0 0 108 285 112 649.0 0 112 286 115 649.0 0 115 287 105 649.0 0 105 288 105 649.0 0 105 289 79 649.0 0 79 290 53 649.0 0 53 291 138 649.0 0 138 292 108 649.0 0 108 293 198 649.0 0 198 294 107 649.0 0 107 295 76 649.0 0 76 296 95 649.0 0 95 297 78 649.0 0 78 298 101 649.0 0 101 299 121 649.0 0 121 300 120 649.0 0 120 301 64 649.0 0 64 Finished in 3.34 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 66,170 Reads with adapters: 19,687 (29.8%) Reads that were too short: 49 (0.1%) Reads written (passing filters): 19,638 (29.7%) Total basepairs processed: 16,588,106 bp Total written (filtered): 5,307,075 bp (32.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19687 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 10 3 19 218 0.0 3 0 201 9 6 2 20 18965 0.0 4 17962 807 148 35 13 21 42 0.0 4 7 32 2 1 22 3 0.0 4 0 0 3 23 59 0.0 4 0 0 0 51 8 24 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 37 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 90 3 0.0 4 0 0 0 0 3 91 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 319 0.0 4 0 0 0 0 319 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 154 6 0.0 4 0 0 0 0 6 158 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 183 2 0.0 4 0 0 0 0 2 198 1 0.0 4 0 0 0 0 1 202 3 0.0 4 0 0 0 0 3 208 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 7 0.0 4 0 0 0 0 7 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.71 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 19,638 Reads with adapters: 4,497 (22.9%) Reads written (passing filters): 19,638 (100.0%) Total basepairs processed: 5,307,075 bp Total written (filtered): 5,258,149 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4497 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 8.7% G: 87.1% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 42 306.8 0 42 4 8 76.7 0 8 6 1 4.8 0 1 7 1 1.2 0 1 8 1 0.3 0 1 9 935 0.1 0 84 851 10 2732 0.0 1 194 2538 11 730 0.0 1 44 686 12 12 0.0 1 0 12 13 8 0.0 1 0 8 14 1 0.0 1 0 1 18 1 0.0 1 0 1 150 9 0.0 1 8 1 152 1 0.0 1 0 1 189 1 0.0 1 0 1 208 8 0.0 1 6 2 209 1 0.0 1 0 1 210 1 0.0 1 1 223 1 0.0 1 1 235 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.