This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.27 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 434,838 (16.4%) Reads that were too short: 46,269 (1.7%) Reads written (passing filters): 388,569 (14.6%) Total basepairs processed: 800,111,879 bp Total written (filtered): 100,425,837 bp (12.6%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 434838 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1265 649.0 0 1265 7 40373 649.0 0 40373 8 32142 649.0 0 32142 9 260 649.0 0 260 10 269 649.0 0 269 11 156 649.0 0 156 12 147 649.0 0 147 13 334 649.0 0 334 14 448 649.0 0 448 15 442 649.0 0 442 16 389 649.0 0 389 17 679 649.0 0 679 18 889 649.0 0 889 19 1502 649.0 0 1502 20 29417 649.0 0 29417 21 18435 649.0 0 18435 22 32470 649.0 0 32470 23 254 649.0 0 254 24 161 649.0 0 161 25 167 649.0 0 167 26 86 649.0 0 86 27 119 649.0 0 119 28 91 649.0 0 91 29 104 649.0 0 104 30 78 649.0 0 78 31 114 649.0 0 114 32 174 649.0 0 174 33 277 649.0 0 277 34 297 649.0 0 297 35 644 649.0 0 644 36 1293 649.0 0 1293 37 318 649.0 0 318 38 403 649.0 0 403 39 799 649.0 0 799 40 378 649.0 0 378 41 374 649.0 0 374 42 668 649.0 0 668 43 725 649.0 0 725 44 986 649.0 0 986 45 853 649.0 0 853 46 1478 649.0 0 1478 47 1970 649.0 0 1970 48 4085 649.0 0 4085 49 71840 649.0 0 71840 50 48025 649.0 0 48025 51 63532 649.0 0 63532 52 277 649.0 0 277 53 60 649.0 0 60 54 46 649.0 0 46 55 43 649.0 0 43 56 69 649.0 0 69 57 68 649.0 0 68 58 80 649.0 0 80 59 36 649.0 0 36 60 90 649.0 0 90 61 76 649.0 0 76 62 138 649.0 0 138 63 1216 649.0 0 1216 64 866 649.0 0 866 65 1573 649.0 0 1573 66 46 649.0 0 46 67 49 649.0 0 49 68 53 649.0 0 53 69 35 649.0 0 35 70 44 649.0 0 44 71 43 649.0 0 43 72 37 649.0 0 37 73 37 649.0 0 37 74 53 649.0 0 53 75 39 649.0 0 39 76 49 649.0 0 49 77 47 649.0 0 47 78 29 649.0 0 29 79 39 649.0 0 39 80 39 649.0 0 39 81 37 649.0 0 37 82 27 649.0 0 27 83 41 649.0 0 41 84 49 649.0 0 49 85 35 649.0 0 35 86 44 649.0 0 44 87 36 649.0 0 36 88 57 649.0 0 57 89 55 649.0 0 55 90 183 649.0 0 183 91 54 649.0 0 54 92 56 649.0 0 56 93 162 649.0 0 162 94 41 649.0 0 41 95 35 649.0 0 35 96 65 649.0 0 65 97 38 649.0 0 38 98 46 649.0 0 46 99 34 649.0 0 34 100 25 649.0 0 25 101 35 649.0 0 35 102 40 649.0 0 40 103 19 649.0 0 19 104 23 649.0 0 23 105 25 649.0 0 25 106 53 649.0 0 53 107 46 649.0 0 46 108 32 649.0 0 32 109 30 649.0 0 30 110 39 649.0 0 39 111 34 649.0 0 34 112 45 649.0 0 45 113 33 649.0 0 33 114 32 649.0 0 32 115 63 649.0 0 63 116 70 649.0 0 70 117 207 649.0 0 207 118 268 649.0 0 268 119 385 649.0 0 385 120 95 649.0 0 95 121 58 649.0 0 58 122 46 649.0 0 46 123 68 649.0 0 68 124 490 649.0 0 490 125 143 649.0 0 143 126 231 649.0 0 231 127 74 649.0 0 74 128 44 649.0 0 44 129 40 649.0 0 40 130 46 649.0 0 46 131 39 649.0 0 39 132 50 649.0 0 50 133 34 649.0 0 34 134 28 649.0 0 28 135 49 649.0 0 49 136 124 649.0 0 124 137 43 649.0 0 43 138 51 649.0 0 51 139 45 649.0 0 45 140 55 649.0 0 55 141 66 649.0 0 66 142 66 649.0 0 66 143 59 649.0 0 59 144 84 649.0 0 84 145 67 649.0 0 67 146 65 649.0 0 65 147 51 649.0 0 51 148 46 649.0 0 46 149 40 649.0 0 40 150 55 649.0 0 55 151 41 649.0 0 41 152 85 649.0 0 85 153 89 649.0 0 89 154 82 649.0 0 82 155 58 649.0 0 58 156 45 649.0 0 45 157 93 649.0 0 93 158 100 649.0 0 100 159 109 649.0 0 109 160 94 649.0 0 94 161 62 649.0 0 62 162 44 649.0 0 44 163 46 649.0 0 46 164 43 649.0 0 43 165 48 649.0 0 48 166 41 649.0 0 41 167 50 649.0 0 50 168 51 649.0 0 51 169 47 649.0 0 47 170 63 649.0 0 63 171 67 649.0 0 67 172 75 649.0 0 75 173 73 649.0 0 73 174 55 649.0 0 55 175 53 649.0 0 53 176 80 649.0 0 80 177 124 649.0 0 124 178 144 649.0 0 144 179 146 649.0 0 146 180 237 649.0 0 237 181 338 649.0 0 338 182 4788 649.0 0 4788 183 420 649.0 0 420 184 2199 649.0 0 2199 185 74 649.0 0 74 186 71 649.0 0 71 187 72 649.0 0 72 188 146 649.0 0 146 189 145 649.0 0 145 190 362 649.0 0 362 191 157 649.0 0 157 192 160 649.0 0 160 193 1301 649.0 0 1301 194 2245 649.0 0 2245 195 2097 649.0 0 2097 196 786 649.0 0 786 197 240 649.0 0 240 198 128 649.0 0 128 199 65 649.0 0 65 200 83 649.0 0 83 201 109 649.0 0 109 202 73 649.0 0 73 203 43 649.0 0 43 204 52 649.0 0 52 205 221 649.0 0 221 206 115 649.0 0 115 207 80 649.0 0 80 208 93 649.0 0 93 209 86 649.0 0 86 210 74 649.0 0 74 211 97 649.0 0 97 212 88 649.0 0 88 213 515 649.0 0 515 214 1670 649.0 0 1670 215 1037 649.0 0 1037 216 1312 649.0 0 1312 217 211 649.0 0 211 218 203 649.0 0 203 219 559 649.0 0 559 220 521 649.0 0 521 221 476 649.0 0 476 222 149 649.0 0 149 223 151 649.0 0 151 224 68 649.0 0 68 225 114 649.0 0 114 226 113 649.0 0 113 227 136 649.0 0 136 228 90 649.0 0 90 229 91 649.0 0 91 230 96 649.0 0 96 231 160 649.0 0 160 232 104 649.0 0 104 233 190 649.0 0 190 234 202 649.0 0 202 235 97 649.0 0 97 236 195 649.0 0 195 237 159 649.0 0 159 238 251 649.0 0 251 239 1097 649.0 0 1097 240 1360 649.0 0 1360 241 1072 649.0 0 1072 242 619 649.0 0 619 243 55 649.0 0 55 244 93 649.0 0 93 245 101 649.0 0 101 246 216 649.0 0 216 247 117 649.0 0 117 248 141 649.0 0 141 249 208 649.0 0 208 250 730 649.0 0 730 251 912 649.0 0 912 252 1197 649.0 0 1197 253 1294 649.0 0 1294 254 149 649.0 0 149 255 91 649.0 0 91 256 171 649.0 0 171 257 341 649.0 0 341 258 215 649.0 0 215 259 421 649.0 0 421 260 253 649.0 0 253 261 284 649.0 0 284 262 288 649.0 0 288 263 2109 649.0 0 2109 264 956 649.0 0 956 265 2531 649.0 0 2531 266 358 649.0 0 358 267 334 649.0 0 334 268 295 649.0 0 295 269 218 649.0 0 218 270 298 649.0 0 298 271 135 649.0 0 135 272 143 649.0 0 143 273 86 649.0 0 86 274 62 649.0 0 62 275 174 649.0 0 174 276 129 649.0 0 129 277 127 649.0 0 127 278 123 649.0 0 123 279 82 649.0 0 82 280 108 649.0 0 108 281 159 649.0 0 159 282 189 649.0 0 189 283 437 649.0 0 437 284 307 649.0 0 307 285 423 649.0 0 423 286 278 649.0 0 278 287 346 649.0 0 346 288 4565 649.0 0 4565 289 2964 649.0 0 2964 290 3566 649.0 0 3566 291 1035 649.0 0 1035 292 182 649.0 0 182 293 284 649.0 0 284 294 317 649.0 0 317 295 241 649.0 0 241 296 94 649.0 0 94 297 258 649.0 0 258 298 202 649.0 0 202 299 271 649.0 0 271 300 321 649.0 0 321 301 245 649.0 0 245 Finished in 18.95 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 388,569 Reads with adapters: 44,057 (11.3%) Reads that were too short: 300 (0.1%) Reads written (passing filters): 43,757 (11.3%) Total basepairs processed: 100,425,837 bp Total written (filtered): 10,907,515 bp (10.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 44057 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 4 18 20 0.0 3 0 0 13 7 19 484 0.0 3 0 425 36 17 6 20 33256 0.0 4 31784 1222 187 43 20 21 71 0.0 4 14 46 3 6 2 22 6 0.0 4 0 1 3 0 2 23 109 0.0 4 4 0 1 77 27 26 1 0.0 4 1 27 5 0.0 4 0 0 0 0 5 28 1 0.0 4 0 0 0 0 1 38 2 0.0 4 0 0 0 0 2 50 5 0.0 4 0 0 0 0 5 52 2 0.0 4 0 0 0 0 2 53 1 0.0 4 0 1 54 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 9 0.0 4 0 0 0 0 9 84 9 0.0 4 0 0 0 0 9 85 7663 0.0 4 0 0 0 0 7663 86 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 88 10 0.0 4 0 0 0 0 10 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 1 96 1 0.0 4 0 0 0 0 1 97 3 0.0 4 0 0 0 0 3 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 108 1 0.0 4 1 110 2 0.0 4 0 0 0 0 2 111 4 0.0 4 0 0 0 0 4 112 9 0.0 4 1 0 0 0 8 113 1 0.0 4 0 0 0 0 1 114 1144 0.0 4 0 0 0 0 1144 116 1 0.0 4 0 0 0 0 1 117 6 0.0 4 0 0 0 0 6 122 5 0.0 4 0 0 0 0 5 124 1 0.0 4 1 125 1 0.0 4 0 0 0 0 1 126 7 0.0 4 0 0 0 0 7 127 873 0.0 4 0 0 0 0 873 128 4 0.0 4 0 0 0 0 4 130 5 0.0 4 0 0 0 0 5 137 1 0.0 4 1 140 6 0.0 4 0 0 0 0 6 145 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 1 1 151 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 156 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 159 2 0.0 4 0 0 0 0 2 160 10 0.0 4 0 0 0 0 10 161 2 0.0 4 0 0 0 0 2 164 2 0.0 4 0 0 0 0 2 165 5 0.0 4 0 0 0 1 4 166 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 0 1 172 3 0.0 4 0 0 0 0 3 173 3 0.0 4 0 0 0 0 3 174 3 0.0 4 0 0 0 0 3 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 2 0.0 4 0 0 0 0 2 179 2 0.0 4 0 0 0 0 2 180 1 0.0 4 0 1 182 1 0.0 4 0 0 0 0 1 183 4 0.0 4 0 0 0 0 4 184 1 0.0 4 0 0 0 0 1 186 65 0.0 4 0 0 0 4 61 187 11 0.0 4 0 0 0 0 11 188 4 0.0 4 0 0 0 0 4 189 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 192 5 0.0 4 0 0 0 0 5 193 3 0.0 4 0 0 0 0 3 194 2 0.0 4 0 0 0 0 2 195 2 0.0 4 0 0 0 0 2 196 3 0.0 4 0 0 0 0 3 197 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 201 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 0 0 1 203 2 0.0 4 1 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 1 207 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 215 15 0.0 4 1 0 0 2 12 216 6 0.0 4 0 0 0 2 4 217 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 219 2 0.0 4 0 0 0 0 2 220 4 0.0 4 0 0 0 0 4 221 2 0.0 4 0 0 0 0 2 222 10 0.0 4 0 0 0 0 10 223 15 0.0 4 0 0 0 0 15 224 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 22 0.0 4 0 0 0 1 21 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 238 2 0.0 4 0 0 0 0 2 239 1 0.0 4 0 0 0 0 1 240 7 0.0 4 0 0 0 0 7 241 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 246 8 0.0 4 0 0 0 0 8 248 2 0.0 4 0 0 0 0 2 249 2 0.0 4 0 0 0 0 2 250 4 0.0 4 0 0 0 0 4 251 2 0.0 4 0 0 0 1 1 252 3 0.0 4 0 0 0 0 3 253 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 269 2 0.0 4 0 0 0 0 2 274 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.51 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 43,757 Reads with adapters: 6,530 (14.9%) Reads written (passing filters): 43,757 (100.0%) Total basepairs processed: 10,907,515 bp Total written (filtered): 10,814,239 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6530 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.0% C: 8.5% G: 77.7% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 83 683.7 0 83 4 54 170.9 0 54 5 11 42.7 0 11 8 1 0.7 0 1 9 17 0.2 0 0 17 10 710 0.0 1 64 646 11 4918 0.0 1 166 4752 12 72 0.0 1 1 71 13 2 0.0 1 0 2 14 13 0.0 1 2 11 16 1 0.0 1 0 1 18 3 0.0 1 2 1 19 348 0.0 1 229 119 20 92 0.0 1 64 28 21 12 0.0 1 9 3 22 13 0.0 1 7 6 23 68 0.0 1 53 15 24 7 0.0 1 5 2 69 1 0.0 1 1 81 4 0.0 1 2 2 94 2 0.0 1 2 106 1 0.0 1 0 1 108 1 0.0 1 0 1 114 1 0.0 1 1 115 1 0.0 1 0 1 121 7 0.0 1 6 1 122 2 0.0 1 1 1 147 1 0.0 1 1 151 2 0.0 1 0 2 152 1 0.0 1 1 175 21 0.0 1 19 2 176 1 0.0 1 1 182 1 0.0 1 1 193 3 0.0 1 2 1 209 5 0.0 1 3 2 210 1 0.0 1 1 211 2 0.0 1 1 1 223 1 0.0 1 1 224 45 0.0 1 38 7 225 1 0.0 1 1